[Sjögren's syndrome associated with a benign thymic tumor: report of a case with wide spectrum of clinical feature (author's transl)].
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Biomedical subjects
Publications and source records attributed to T Uchida.
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The purpose of this paper was to investigate whether cardiovascular afferent impulses could be recorded on a human scalp as computer-averaged cortical potentials time-locked to EKG-R waves. The results obtained were as follows. 1) Cortical potentials time-locked to EKG-R waves (EKG-EP) could be recorded on scalps of eleven healthy volunteers and within its pattern, triple negative large peaks could be seen during both wakefulness and slow wave sleep (stage 2). 2) Within triple negative large peaks, the 2nd negative peak were most stable and its averaged latencies were 224.1 +/- 32.4 msec. during wakefulness and 257.1 +/- 76.7 msec. during slow wave sleep (stage 2) and the 3rd negative peak had been found more frequently in the slow wave sleep than in the wakefulness. 3) EKG-EPs were analogous with those of auditory evoked potentials time-locked to EKG-R waves (EKG-AEP) if both were recorded in the same concious level. 4) Both latencies of the 2nd negative peak of the EKG-EPs and that of the largest negative peak of the EKG-AEPs were consistent each other with high ratio resulting r = 0.81 (P less than 0.01) in the wakefulness and r = 0.966 (P less than 0.001) in the slow wave sleep (stage 2) respectively.
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For this study, spontaneous K-complex (spo-K) during slow wave sleep (stage 2) were observed on eleven normal subjects ranged for 17 to 37 years of age. And the cardiac and respiratory cycles of two physiological rhythms were simultaneously recorded with EEG to investigate the occurrences of spo-K. 1) The spo-K occurrences time-locked to EKG-R waves during both inspiration and expiration were found to have triple peak latencies of distribution such as 200--300, 400--600 and 800--900 msec. respectively (analytic time: 1000 msec.). 2) As above mentioned, these triple peak latencies of spo-K were corresponded respectively with those of computer-averaged cortical potentials time-locked to EKG-R waves such as Nw, Nx and Ny which were simultaneously recorded. 3) The types of spo-K were divided into three groups such as A, B and C types. A and B of spo-K were attributed to interaction of cardiovascular and respiratory afferent impulses respectively. Otherwise, C type of spo-K was attributed to other unknown visceral afferent impulses or unknown brain mechanism. 4) Spo-K appeared during sleep corresponding with both hiccup and EKG in a 28-year-old epileptic patient with hiccup. In our studies, we made special attention of cardiorespiratory nervous system aimed at the genesis of spo-K occurrences.
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The body sway in upright standing was decreased by periodic saccades. The decrease was also observed during voluntary rapid eye movements in complete darkness, indicating that the visual information was not concerned with the decrease of the sway. Continuous eye rotations did not affect the body sway, showing that the repetitive activation of eye muscle proprioceptors was not the cause of the sway reduction. These results suggest that some information related to the execution of saccades may affect the spinal motor system and cause the stabilization of the standing posture.
A mixture of HVJ (Sendai virus) spike proteins, the nontoxic fragment A of diphtheria toxin, lecithin, and cholesterol was solubilized in sucrose solution containing a nonionic neutral detergent. The liposomal vesicles which formed on removal of the detergent by dialysis were purified by gel filtration and centrifugation on a sucrose gradient. The resulting purified vesicles had hemagglutinating activity, hemolytic activity and, after solubilization, the enzymic activity of fragment A. The vesicles had no cell fusion activity. Electron microscopy showed that both the outside and inside of membranes of the vesicles were associated with the spikes. When the vesicles were freeze-fractured, no large aggregates of particles were seen on either face. Such fragment A-containing lipid vesicles (liposomes) with HVJ spikes bound to mamalian cell membrane and released their fragment A into the cytoplasm causing cell death. Neither fragment A-containing liposomes without spikes nor empty liposomes with spikes were toxic.
A new cultured cell line (HuOS) was established from tumor cells obtained from the pulmonary metastatic foci of a patient diagnosed as chondroblastic osteosarcoma. The tumor cell line was maintained for over 19 months, and morphological and biological characteristics were studied. These cells retained their malignant properties and produced nodules when transferred intramuscularly to nude mice. Morphologically, these nodules revealed a chondromatous pattern.
A nonlysogenic, non-toxinogenic strain was isolated from the PW8 strain of C. diphtheriae by two-step ultraviolet induction. This strain was lysed by phages derived from the PW8 strain but not by beta phages from the C7(beta) strain. When this cured strain was lysogenized with phages from the PW8 strain, toxin production by the resulting lysogens was about a half or a quarter of that of the parent strain PW8.
In order to detect the precise location and pathways of calcium in the ameloblastic layer with EM and EDX, frozen ultrathin-sections, potassium pyroantimonate (PPA) method and 45Ca-autoradiography (A.R) were employed. A population of dense granules containing Ca and P was mainly observed within the nuclei and mitochondria of the frozen-sectioned ameloblasts, whereas PPA-reaction was seen not only within them but in ER, Golgi vesicles and intercellular spaces. No granules were found at the enamel matrix by both methods, but peaks for Ca and P were distinct. Silver grains of 45Ca-A.R were located in nuclei, mitochondria. Golgi complex and intercellular spaces. These results indicate that calcium can transport across the ameloblastic layer through two main pathways, extracellular and transcellular.
A platelet kinetic study in 2 patients with acute promyelocytic leukemia (APL) associated with disseminated intravascular coagulation (DIC) demonstrated that the platelet survival was characteristically shortened. These findings suggest increased platelet consumption during the process of DIC in APL.
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