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Biomedical subjects

T Tsuji

Publications and source records attributed to T Tsuji.

At least 955 records · Page 53Linked to original sources
PubMed1987

A unique amino acid sequence of the B subunit of a heat-labile enterotoxin isolated from a human enterotoxigenic Escherichia coli.

The purified B subunit of heat-labile enterotoxin produced from a human strain, 240-3, of enterotoxigenic Escherichia coli (LTh(240-3] was carboxymethylated, succinylated, digested with chymotrypsin and subjected to high performance liquid chromatography (HPLC), and the amino acid compositions of the peptide peaks from the column were analyzed and compared with the data reported by Yamamoto and Yokota (J. Bacteriol. 155, 728.1983), who deduced the amino acid sequence of LTh(H10407) from the DNA sequence of a human strain H10407. Only one fraction differed in amino acid composition from that reported by them. This fraction was found to consist of peptides with the sequences Arg-Asn-Thr-Gln-Ile-Tyr and Arg-Ile-Ala-Tyr. Yamamoto and Yokota reported the sequence of the latter peptide as Arg-Ile-Thr*-Tyr, which corresponds to the peptide from 73rd to 76th from amino (N-) terminus. Thus amino acid residue 75 from the N-terminus of LTh-B(240-3) is alanine, not threonine. The B subunit of cholera toxin also has alanine at position 75. LTh(240-3) appeared similar to LTh(H10407) in an Ouchterlony test, vascular permeability test and GMI ganglioside ELISA. These data show that substitution of threonine for alanine at position 75 from the N-terminus does not affect the immunological and biological characteristics of LTh.

Amino Acid Sequence↗
PubMed1987

The carbohydrate moiety of human platelet glycocalicin: the structures of the major Asn-linked sugar chains.

Glycocalicin, a predominant glycoprotein on the human platelet surface, has been purified from a platelet suspension by means of sonication, ammonium sulfate precipitation and acid treatment followed by chromatography on columns of wheat germ agglutinin-Sepharose and Mono Q. Asparagine-linked (N-linked) oligosaccharides were released by hydrazinolysis, and then N-acetylated and reduced with NaBH4 or NaB3H4. The released carbohydrate chains were found to be of the complex-type from their interaction with immobilized lectin columns. The structures of the two major oligosaccharide-alditols separated by ion-exchange chromatography on a Mono Q column were investigated by means of methylation analysis, glycosidase digestion, and Smith periodate degradation, and they were assigned as typical di- and trisialylated complex-type oligosaccharide-alditols with two and three peripheral chains consisting of Gal-GlcNAc sequences, respectively.

Amino Acids↗
PubMed1987

A micromethod for the isolation of large and small microvessels from frozen autopsied human brain.

Microvessels were isolated from autopsied human brain using a simple procedure involving disruption, sieving, and centrifugation on a sucrose density gradient. The present procedure is characterized by isolation, from frozen autopsied brain, of materials either from the cerebral cortex or white matter, and subsequent separation of the capillary fraction from the large vessel fraction. The preparation appears highly purified under phase-contrast microscopic examination. The purity was also established by the enrichment of gamma-glutamyl transpeptidase activity and by the nearly negligible cerebroside content in the vessel fractions as compared to the brain homogenate.

Brain↗
PubMed1987

Elastic fibers in scar tissue: scanning and transmission electron microscopic studies.

Cutaneous scar tissue (atrophic and hypertrophic) and normal dermis were examined using scanning (SEM) and transmission electron microscopy (TEM) as well as light microscopy (LM). Although LM revealed only a few Weigert stain-positive elastic fibers in scar tissue, SEM and TEM disclosed the presence of a large number of fine elastic fibers which was not present in normal dermis. Atrophic scar tissue contained more Weigert stain-positive fibers and fewer fine elastic fibers than hypertrophic scar tissue. In addition, finding suggestive of newly synthesized elastic fibers were seen in some hypertrophic scar tissue. These results indicate that there is elastogenesis in scar tissue.

Adolescent↗
PubMed1987

Ultrastructure of deep wrinkles in the elderly.

Deep wrinkles of 6 elderly people were investigated using electron microscopy. There were ultrastructural differences in the elastotic changes between the area of the wrinkle and the surrounding areas. Elastic fibers containing electron-dense inclusions were mostly seen in the upper dermis of the wrinkled area and were considered to be an early event of the elastotic changes. The elastotic changes showing net-like structures were seen in the areas surrounding the wrinkle and were considered to be a more advanced event. Another form of elastotic change was the presence of thickened elastic fibers with many holes containing round bodies, which were scattered in the deep dermis of the wrinkled area, and increased and packed in the middle and deep dermis of the surrounding areas. In one case subepidermal amyloid deposits were seen in the areas surrounding the wrinkle, suggesting a close relationship between amyloid and sun-light exposure.

Aged↗
PubMed1987

Different effects of elastase on dermal elastic fibers with age. Ultrastructural studies with the conventional and tannic acid stain.

The effect of elastase on dermal elastic fibers from three age groups (children, young and old adults) has been investigated using ultrathin sections embedded in Epon. There were significant differences in the effect of elastase among the age groups; younger elastin was more rapidly digested by elastase than older elastin. In children and young adults, digested areas by elastase were first recognized as tiny round holes scattered in the amorphous material of the elastic fiber, extending to the large part of the fiber except for microfibrils. In old adults, digested areas were limited to the only small part of the elastic fiber.

Actin Cytoskeleton↗
PubMed1987

Mechanism of vascular responsiveness to barbiturates in isolated and perfused canine basilar arteries.

We inserted stainless steel cannulae to compare vascular responses to five kinds of barbiturates and to investigate the mechanism of vascular responsiveness to thiopental in isolated and perfused canine basilar arteries. Intraluminal thiopental (10 to 3000 micrograms) usually caused a distinctly biphasic vascular response, i.e., initial vasoconstriction followed by vasodilatation. The vasoconstriction was always greater than the vasodilatation in percentage of change. Thiamylal (10 to 3000 micrograms) evoked similar vascular responses. Pentobarbital, phenobarbital, and amobarbital (100 to 3000 micrograms) induced slight monophasic vasodilatation in lower doses and a weak biphasic response in higher doses. Pretreatment with phentolamine and ketanserin, an alpha-adrenoceptor antagonist and a 5-hydroxytryptamine2 inhibitor, did not alter vasoconstriction to thiopental. Increasing doses of pretreatment with diltiazem, a potent calcium antagonist, depressed the vasoconstriction induced by potassium chloride in a dose-related manner, but thiopental-induced constriction was only slightly suppressed by diltiazem treatment. Endothelial removal produced by intraluminal saponin treatment significantly enhanced vasoconstriction to potassium chloride, but thiopental-induced constriction was not enhanced significantly after saponin treatment. These findings suggest that thiopental-induced vasoconstriction does not involve adrenergic and serotonergic mechanisms and is due to intracellular movement of calcium ions in canine basilar arteries.

Animals↗
PubMed1987

Fractionation of human milk oligosaccharides by high-performance liquid chromatography.

An ion-exchange chromatographic system was used to isolate several human milk oligosaccharides, the elution being carried out with a linear gradient of a sodium borate buffer. Lacto-N-tetraose, lacto-N-neotetraose, lacto-N-fucopentaose I, II and III, lacto-N-difucohexaose I and 2'-alpha-fucosyllactose can be separated by this method.

Chromatography, DEAE-Cellulose↗
PubMed1987

The effect of timing of clot removal on chronic vasospasm in a primate model.

The effect of complete clot removal at times from 48 to 96 hours after subarachnoid hemorrhage (SAH) on the development of chronic cerebral vasospasm was evaluated to determine whether there is a critical point after which clot removal is ineffective in preventing vasospasm. Thirty cynomolgus monkeys were randomized to one of five groups: sham-operated group, clot removal at 48 hours after SAH (48-hour group), clot removal at 72 hours after SAH (72-hour group), clot removal at 96 hours after SAH (96-hour group), and clot placement only (clot group). Standard microsurgical techniques were used to dissect bilaterally the major cerebral arteries free of arachnoid. An autologous blood clot averaging 4.2 gm was placed around the vessels in the subarachnoid space of the monkeys in the 48-hour, 72-hour, 96-hour, and clot groups. Physiological saline was instilled into the subarachnoid space of the sham-operated animals. Animals in the clot-removal groups underwent surgical clot removal at the determined times for each group. Two animals in each of the sham-operated and clot groups were subjected to reoperation at each of 48, 72, and 96 hours after SAH. The incisions were reopened and then simply reclosed. Neurological status, angiographic cerebral vessel caliber, and physiological status were evaluated before and 7 days after SAH induction. There were no significant neurological deficits in the sham-operated, 48-hour, or 72-hour groups. Two animals in each of the 96-hour and clot groups showed deterioration in level of consciousness developing on Day 4 or 5 after SAH induction. All the major cerebral arteries of the animals in the clot and 96-hour groups showed significant vasospasm (p less than 0.01) on Day 7. Animals in the 72-hour group had significant vasospasm (p less than 0.05) of the internal carotid and middle cerebral arteries but not the anterior cerebral arteries. There was no significant vasospasm (p greater than 0.05) in any of the cerebral arteries in the 48-hour group. Severity of vasospasm paralleled the duration of contact between the blood clot and the cerebral vessels. Evacuation of the subarachnoid hematoma later than 48 hours after SAH resulted in no significant reduction in the degree of chronic cerebral vasospasm. It is suggested that clot removal at early operation is likely to be useful only if it is performed within 48 hours of SAH.

Animals↗
PubMed1987

Acquired cutis laxa concomitant with nephrotic syndrome.

A 41-year-old woman developed laxity and wrinkling of the skin. This process spread slowly to involve the skin of almost the entire body, without any previous inflammatory skin disorder. The skin of her face, neck, and trunk hung in loose folds, leading to a prematurely aged appearance. One year later she noticed edema on her face and legs. Laboratory studies disclosed low C3 and CH50 serum levels and proteinuria. Skin biopsy specimens revealed extensive loss and fragmentation of dermal elastic fibers. A renal biopsy specimen showed membranoproliferative glomerulonephritis (type 2), and an immunofluorescent study disclosed C3 and IgG deposition in the mesangial matrix and along the glomerular basement membrane. We propose that, in this case, cutis laxa may be related to an abnormal immune response.

Adult↗
PubMed1987

[Observation of the superior mesenteric artery by ultrasonography: for prediction of the superior mesenteric artery syndrome].

Superior mesenteric artery (SMA) syndrome is a major complication after scoliosis surgery. The author reports an ultrasonic observation of the SMA in normal and scoliotic individuals. There are three patterns of the SMA anatomy: Type I in which the SMA runs very close and parallel to the aorta, Type II in which the SMA branches off the aorta at a certain angle and Type III in which the SMA runs parallel to the aorta. Dynamic observation revealed that passage of food displaces the SMA anteriorly and to the left and that turning to the left from a supine position displaces the SMA from just anterior to the left of the aorta in normal individuals and from the right to the left side of the aorta in scoliosis cases. AMA itself has no significance in the occurrence of SMA syndrome because the SMA moves rather freely with the passage of food or position change. When this natural movement of the SMA is disturbed, one should carefully look for the possibility of SMA syndrome.

Adolescent↗
PubMed1987

[An expression of an SCC antigen in oral cancer].

An expression of a squamous cell carcinoma-related antigen (SCC antigen) in the blood and cancer tissue has been examined in oral cancer patients by radioimmunoassay and immunocytochemistry respectively. The antigen levels in the serum proved to be high in the cases in an advanced stage and/or showing a poorly differentiated type while, immunocytochemically, carcinomas of a well differentiated type stained well with a monoclonal anti-SCC antibody. This suggests that, as there is discrepancy between the levels of serum and tissue, an expression of the antigen seems to depend on the biological characteristics of cancer cells themselves.

Adult↗
PubMed1987

[A complete response in squamous cell carcinoma of the lung to combination chemotherapy by cisplatin and vindesine].

A 75-year-old male was admitted to our hospital for further investigation of a large mass shadow in the left hilum that was seen on an X-ray film of his chest. Cytological examination of his sputum revealed keratinized squamous cell carcinoma and combination chemotherapy using cisplatin and vindesine was administered. Later, both a chest X-ray and a CT scan showed a remarkable reduction in the size of the lung cancer. Complete response was obtained on the 90th hospital day, and was maintained with UFT administration. No sign of recurrence has been observed for over 9 months.

Aged↗