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Biomedical subjects

T Tsuchida

Publications and source records attributed to T Tsuchida.

At least 343 records · Page 19Linked to original sources
PubMed1981

[Studies on toxicity of hydrocortisone 17-butyrate 21-propionate -3. Subacute toxicity in rats by percutaneous administration (author's transl)].

Subacute toxicity of hydrocortisone 17-butyrate 21-propionate (HBP) was studied in rats. HBP was percutaneously given to rats with 0.1% and 0.5% creame (0.1% HBP-C, 0.5% HBP-C) and ointment (0.1% HBP-O, 0.5% HBP-O) at the daily dose level of 150 mg per 100 g body weight for 1 month. Rats receiving HBP-C and HBP-O showed some dose-dependent symptoms such as the suppression of body weight gain, emaciation, decrease in the number of white blood cells, hemoglobin, hematocrit, and serum total cholesterol level, regressive changes in adrenals, skin, lymphatic and hematopoietic tissues, which are known as toxic effects of synthetic corticosteroids. These symptoms were comparatively high toxic in male rats and in cream groups, and almost disappeared in rats elapsed recovery time of 1 month after withdrawal of HBP.

Administration, Topical↗
PubMed1981

[Studies on toxicity of hydrocortisone 17-butyrate 21-propionate -4. Chronic toxicity in rats by subcutaneous administration (author's transl)].

Chronic toxicity of hydrocortisone 17-butyrate 21-propionate (HBP), a new synthetic corticosteroid, was studied in rats. HBP was subcutaneously injected to rats as the daily doses of 0.001, 0.01, 0.01, 1.0 and 3.0 mg/kg for 6 months, and the following recovery test was carried out for 4 weeks. Hydrocortisone 17-butyrate (HB) and betamethasone 17-valerate (BV) were used as the reference drugs at the doses of 0.1, 1.0 and 3.0 mg/kg. The suppression of body weight gain by the administration of HBP was observed at the doses more than 1.0 mg/kg in male and more than 0.1 mg/kg in female, and the dead animals were sent at the highest dose of HB and BV. Mainly at the doses more than 0.1 mg/kg HBP induced the dose-dependent symptoms such as decrease in the number of white blood cells and total protein level in serum, and increase in total cholesterol, GOT and GPT level in serum, and atrophic changes of adrenals, lymphatic tissues, skin and subsexual organs. No usual abnormality was recognized at the doses less than 0.01 mg/kg of HBP. These symptoms were more toxic in male, and the strength of toxicity was in the order of BV greater than HB greater than HBP. Many of these findings have known as common effects of corticosteroids. The changes observed in this study were almost recovered after withdrawal of HBP at the doses less than 0.1 mg/kg. As the result, it was suggested that the maximum non-toxic dose of HBP was 0.001 mg/kg.

Administration, Topical↗
PubMed1981

[Studies of toxicity of hydrocortisone 17-butyrate 21-propionate -5. Chronic toxicity in rats by percutaneous administration (author's transl)].

Chronic toxicity of a new synthetic corticosteroid, hydrocortisone 17-butyrate 21-propionate (HBP), was investigated in rats of both sexes. HBP was percutaneously given to rats with 0.1%, 0.5% cream and ointment at the daily dose level of 150 mg per 100 g body weight for 6 months. For the comparison, the percutaneous toxicity with 0.12% betamethasone 17-valerate (BV) cream and ointment, and 0.1% hydrocortisone 17-butyrate (HB) cream and ointment at the daily dose level of 150 mg per 100 g body weight were studied. Rats receiving HBP showed the dose-dependent changes such as the suppression of body weight gain and food intake, emaciation, decrease in the number of white blood cells and lymphocytes, total protein, increase in the number of red cells, hematocrit, hemoglobin, blood sugar and total cholesterol, regressive changes in adrenal cortex, lymphatic and hematopoietic tissues and skin, and gastric erosion, which have been well known as toxic effects of synthetic corticosteroids. These findings were comparatively high toxic in male, and almost disappeared in rats elapsed recovery time of month after withdrawal of HBP. The toxicities of HBP, BV and HB were qualitatively same. However, the grade of effects of HBP toxicity was similar to that of HB, weaker than of BV.

Administration, Topical↗
PubMed1981

The mechanism of specific suppression in effector T cell clones against tumor-associated transplantation antigens.

The present study investigates the fate of effector T cell population against tumor-associated transplantation antigens (TATA) of X5563 plasmacytoma in syngeneic mice rendered specifically unresponsive to the TATA. In this tumor system T cell-mediated tumor-specific immunity was induced by intradermal inoculation of viable tumor cells followed by the surgical resection of the tumor (immunization procedure). The intravenous (iv) presensitization of syngeneic hosts with X-irradiated tumor cells abolished the capability of those hosts to develop the tumor-specific immunity after the above appropriate immunization procedure. Spleen cells from the pretreated mice which subsequently received the immunization procedure could not regain the tumor-neutralizing activity after enzymatic treatment with papain. Moreover, lymphoid cells from the pretreated mice could not be stimulated by the immunization procedure even after proteolytic treatment with papain or trypsin, followed by transfer into other recipient mice free of the serum suppressive factor(s). On the other hand, such enzyme treatment was capable of preventing the tolerance induction of dinitrophenyl (DNP)-primed B cells after in vitro pulsing with DNP-D-GL (copolymer of D-glutamic acid and D-lysine) for 2 hr, suggesting that the enzymatic treatment used here was adequate to remove the blocked receptor and any tolerogen. These results suggest that in X5563 plasmacytoma system, the above specific unresponsiveness induced by the iv presensitization with TATA is due to the irreversible inhibition or deletion of effector T cell clones rather than mere effector cell blockade.

Animals↗
PubMed1981

[The prognosis of encephaloceles (author's transl)].

Encephaloceles were diagnosed in 39 patients over a 40-year interval from 1940. Thirty-three encephaloceles were located in the occipital region, 3 in parietal, 1 in glabellar, 1 in sphenomaxillar and 1 in intracranial. Nineteen patients were males and 20 were females. Eleven of 39 patients were dead. In 33 patients the encephalocele was excised and two died within a month after operation. Four patients expired within a year and other four died before the age of 4 years. Eleven of dead cases were meningoencephaloceles or meningoencephalocystoceles except one. In 6 of them the size of encephalocele was larger than 5 cm in diameter. Twenty-six patients survived 6 months fo 34 years. Six of them found to be severely retarded psychosomatically. Twenty others spend normal lives. Eldest patient whose occipital meningoencephalocele sized 4.5 cm in diameter was excised in 1945 works in a automobile dealer's shop as a car operator after graduation of a junior high school. He has two children, as well. Two patients are graduates of a high school, one a college and one a junior high school. Other patients of school age attend ordinary classes of each school with or without minimal handicaps such as paresis of one leg, unilateral visual loss, slight cerebellar ataxia, large head size and so on. Two of these 20 patients who are found to be in good state had encephaloceles larger than 5 cm in diameter, but other 18 smaller than 5 cm. Eleven of them were meningoceles. Four of 6 patients who had anterior or parietal encephalocele were found to have no neurological sequelae. Significant adverse prognostic factors were the presence of brain tissue within the sac of lesion and the size of it. However, hydrocephalus did not effect the quality of survival of our patients.

Adolescent↗
PubMed1981

[A case of deep sylvian meningioma with intracerebral hematoma (author's transl)].

A case of deep sylvian meningioma is reported which is unusual in the aspect that the initial symptoms were headache and vomiting due to intracerebral hemorrhage from meningioma. After evacuation of hematoma in the right temporal lobe and biopsy of the tumor, it was found to be meningothelial meningioma with psammoma bodies. Cerebral angiography showed characteristic findings of intrasylvian vascular tumor. Complete removal of this tumor was performed under microscopy, dissecting dense adhesion between the tumor and main branches of the middle cerebral artery. Consequently, the patient showed only slight impairment of fine movement of his left fingers. Deep sylvian meningioma designated by Cushing and Eisenhart is very rare. This case is the only one of deep sylvian meningioma in our whole series of 181 intracranial meningiomas and probably the twentieth case reported so far in the literature.

Cerebral Angiography↗
PubMed1981

Inhibitory effect of Propionibacterium acnes-activated macrophages on the tumor metastasis enhanced by tumor-specific immunosuppression.

The present study deals with the mechanism by which Propionibacterium acnes (P. acnes) prevents metastasis formation in C3H/He mice which had been rendered tolerant to tumor-associated transplantation antigen (TATA) of syngeneic X5563 plasmacytoma by intravenous (iv) inoculation of 10(6) X-irradiated tumor cells 3 times at 4-day intervals. In these TATA-tolerant mice, systemic metastasis formation was enhanced after intradermal (id) implantation of viable tumor cells, even if the tumor was resected surgically 7 days thereafter. Administration of P. acnes to these TATA-tolerance mice induced appreciable prevention of the metastasis formation. When we passively transferred P. acnes-activated peritoneal exudate cells (PECs) instead of P. acnes into TATA-tolerant mice, successful prevention to metastasis was also observed, irrespective of whether they were derived from TATA-tolerant mice or from normal naive mice. In both in vivo and in vitro assay systems, the P. acnes-activated PECs from TATA-tolerant mice as well as from normal naive mice showed potent anti-tumor activity. In the in vitro assay, anti-tumor effector cells in P. acnes-activated PECs were found to reside in a plastic adherent population resistant to treatment with anti-Thy-1 plus complement. These results are consistent with the view that effector cells for the prevention of metastasis are activated macrophages. The significance of these findings is discussed in relation to the possibility of the future application of an immunomodulator which activates macrophages to prevent metastasis.

Animals↗
PubMed1980

Preventive effect of Propionibacterium acnes on metastasis in mice rendered tolerant to tumor-associated transplantation antigens.

The present study was carried out to investigate the preventive effect of Propionibacterium acnes (P. acnes) on metastasis formation of C3H/He mice which has been rendered tolerant to tumor-associated transplantation antigen (TATA) of syngeneic X5563 plasmacytoma. C3H/He mice inoculated intravenously (iv) with 10(6) 7000 R X-irradiated X5563 tumor cells 3 times at 4-day intervals. These pretreated mice could not develop immune resistance against X5563 tumor even after the appropriate immunization procedure. In these TATA-tolerant mice, enhanced metastasis associated with the development of visually apparent metastatic nodules in the spleens was observed after intradermal (id) implantation with viable tumor cells, even if the tumor was resected surgically 7 days thereafter. Administration of P. acnes to these TATA-tolerant mice appreciably reduced the metastasis formation. Although the growth of the id primary tumor was not affected by injection with P. acnes, the combined treatment of surgical resection of the primary tumor and P. acnes administration resulted in complete protection against metastasis in TATA-tolerant mice. These results unequivocally demonstrated that the administration of P. acnes is effective for preventing the outbreak of metastasized tumors, which are readily inducible in the TATA-tolerant state.

Animals↗
PubMed1979

Further studies on the electrophoretic pattern of albumin in diabetic sera.

Human sera from normal subjects and diabetic patients were electrophoresed in an urea-containing gel; polyacrylamide gel and cellulose acetate membrane. In polyacrylamide gel, normal serum exhibited the fast migrating Band 2 and the slower migrating albumin Bands 4 and 5 after overnight fasting and also after glucose administration. In diabetic serum Bands 4 and 5 did not appear before or after glucose administration in low mercaptoethanol gel which abnormality resembled the pattern in C57BL/KsJ-db/db mice. In high mercaptoethanol gel, about half of the diabetic sera exhibit a delay in appearance of Bands 4 and 5, i.e., Bands 4 and 5 were not observed 30--60 min after glucose administration, which seems to resemble the pattern in C57BL/6J-ob/ob mice. Conditions of electrophoresis in urea-submerged cellulose acetate membrane (species of buffer systems, pH, ion concentration, mercaptoethanol, ethylene-diaminetetraacetic acid, Ca2+ ion, urea concentration, etc.) were observed in relation to albumin sub-separation. At pH 8.6 with barbital buffer, albumin separated into two bands, and at pH 10.6 with glycine buffer, albumin separated into four bands. Almost all diabetic sera (ca. 80%) exhibited different electrophoretic patterns from that of normal serum.

Animals↗