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T Tsuchida

Publications and source records attributed to T Tsuchida.

At least 271 records · Page 15Linked to original sources
PubMed1989

Familial occurrence of impaired interleukin-2 activity and increased peripheral blood B cells actively secreting immunoglobulins in systemic lupus erythematosus.

PURPOSE: We tested the hypothesis that some abnormalities of immune functions are genetically controlled in patients with systemic lupus erythematosus (SLE). SUBJECTS AND METHODS: We used a phytohemagglutinin-induced interleukin-2 (IL-2) activity assay and a spontaneous plaque-forming cell assay to evaluate T-cell and B-cell function, respectively, in 34 clinically healthy family members of six SLE probands. RESULTS: Impaired IL-2 activity was found in 15 of the 29 consanguineous relatives. There was no relation between the household relatives and the nonhousehold relatives; none of the five nonconsanguineous household persons had abnormal results. Results for the B-cell assay were abnormal in 22 of the 29 consanguineous relatives. The B-cell abnormalities were more commonly observed in the consanguineous household relatives; four of the five nonconsanguineous household relatives also had abnormal assay results. CONCLUSION: The findings indicate that the impaired IL-2 activity in relatives appears to strongly correlate with a genetic relationship. Although the evidence favors a genetic basis for the B-cell abnormalities, environmental effects may also contribute to the familial occurrence of these abnormalities.

Adolescent↗
PubMed1989

[Retrograde apicoectomy. Development of a new technique and its clinical application].

We developed a new technique instead of usual retrograde apicoectomy for cases in which apical lesions appear after prosthetic treatment. The technique is as follows. After treatment of the apical area by raising a flap, part of the labial bone is removed and the labial surface of the root is exposed. Then the labial part of the root is removed and the root canal is opened. After that, the residual carious dentin is removed completely. At the final stage, the open root canal is filled with tooth adhesive and low viscosity composite resin (Clearfil-SC). This technique is indicated for cases in which the crown restoration is not removed for some reasons and treatment from apical side is not possible. This technique is clearly the last choice, but many teeth will remain.

Apicoectomy↗
PubMed1989

Fluctuation of the mucosal hydroxyproline content in compound 48/80-induced gastric lesions in rats.

Extensive gastric mucosal lesions were observed after i.p. administration of compound 48/80 (0.75 mg/kg) for 3 days to rats. The mucosal hydroxyproline content was significantly increased at 4 h after the last injection of compound 48/80 and subsequently decreased as the lesions were healed. Treatment with cimetidine (50 mg/kg, i.p.) did not prevent compound 48/80-induced gastric lesions. The increase in the mucosal hydroxyproline content was significantly reduced by treatment with cimetidine. This fact suggested that cimetidine might have an inhibitory effect on the regeneration of connective tissue.

Animals↗
PubMed1989

The role of prostaglandin D2 in the genesis of indomethacin-induced gastric lesions in rats.

Four kinds of prostaglandins (PGs), 6-keto-PGF1 alpha, PGF2 alpha, PGE2 and PGD2, in rat gastric mucosa were decreased at 1 or 6 h after oral administration of indomethacin (2 or 12 mg/kg). With 2 mg/kg of indomethacin, the PG levels had slightly recovered 6 h later. Gastric lesions were observed 6 h after administration of indomethacin (12 mg/kg), but not with 2 mg/kg. Omeprazole (20 mg/kg, i.p.) prevented ulcer formation caused by indomethacin, without improvement of the reduced gastric mucosal PG levels. PGD2 (0.5 mg/kg, p.o.) reduced indomethacin-induced gastric lesions and considerable amounts of PGD2 existed in the gastric mucosa. We conclude that H+ is a determining factor in the genesis of indomethacin-induced gastric lesions and persistent decreases in tissue PG levels also participate in ulcer formation.

Animals↗
PubMed1989

[Two cases of infantile subungual pigmented nevi with Hutchinson's sign].

Two cases of subungual pigmented nevi with periungual melanotic freckles, namely Hutchinson's sign, are presented. They are a 4-year-old female and a 11-month-old male; in neither case did the mother notice the lesion at birth. Histologically, both cases were characterized by the presence of well-circumscribed small nests of nevus cells in the periungual melanotic areas; diagnoses of junctional nevus and compound nevus were made, respectively. However, atypical proliferations of melanocytes at the dermo-epidermal junction of the nail matrix and nail bed were observed in some areas. The measurement of the DNA content of melanocytes by means of microfluorometry revealed normal diploid pattern in the former case, but the latter case contained a few aneuploid cells, suggesting that the proliferating melanocytes might have malignant potentials. Infantile subungual pigmented nevi with Hutchinson's sign must be examined thoroughly and watched carefully.

Aneuploidy↗
PubMed1989

[Morphological study on open bite patients].

The purpose of the present study is to investigate the morphological features of open bite patients who have also disharmony of anteroposterior relations between maxilla and mandible. Lateral head films of 40 adult females with open bite were used. The samples were classified into three groups by ANB angle. Three groups were as follows. 1. Type I open bite (12 subjects, 1 degree less than or equal to ANB less than or equal to 4.5 degrees). 2. Type II open bite (13 subjects, ANB less than 4.5 degrees). 3. Type III open bite (15 subjects, ANB less than 1 degree) The normal samples was the longitudinal data of females in our department. Skeletal and dental variables were calculated and compared norm versus type I open bite, type I open bite versus type II open bite and type I open bite versus type III open bite, respectively. The following results were obtained. 1. Disharmony of the vertical dimensions in type I open bite was mainly due to long anterior lower facial height which was accompanied by large gonial angle as compared with norm. 2. There were no difference in anteroposterior position of the maxilla and nasal floor in all three open bite groups. 3. There were no difference in anterior and posterior facial height between type I and type II open bite. However, type II open bite showed the backward rotation of the mandible accompanied by over eruption of upper first molars. 4. Type III open bite showed longer anterior lower facial height which was caused by excessive mandibular growth than type I open bite. 5. Upper incisors showed labial inclination as compared with norm in all three open bite groups. However, lower incisors showed labial inclination in type II open bite and lingual inclination in type III open bite as compared with type I open bite. As a result, the inter-incisal angle was small in type II open bite and large in type III open bite as compared with type I open bite. These results suggested that the dentoalveolar factors were large in type II open bite and the skeletal factors were large in type III open bite as compared with type I open bite.

Cephalometry↗
PubMed1988

Intracellular activation signal requirements for the induction of IL-2 responsiveness in resting T cell subsets in humans.

Activation signal requirements for the induction of the IL-2 responsiveness in purified subsets of human resting T cells, T4+ or T8+, have been investigated under the monocyte-depleted conditions. Substantial levels of IL-2 responsiveness were induced in T8+ cells by lectin, Con A, mAb directed against the CD3 Ag, OKT3, Ca2+ ionophore, ionomycin or phorbol ester, PMA. In contrast, none of these stimuli was by itself sufficient for the induction of IL-2 responsiveness in the T4+ subset. The latter cells could, however, be induced to respond to IL-2 by combinations of PMA plus either of Con A, OKT3, or ionomycin (but not any combination of Con A, ionomycin, and OKT3). These data indicate that induction of IL-2 responsiveness in the resting T4+ subset is more complex, possibly requiring two intracellular activation signals, increase in the concentration of intracellular Ca2+ and activation of protein kinase C, whereas either signal may directly trigger IL-2 responsiveness in the resting T8+ cells. The data further suggest that under optimal conditions, growth of both resting T4+ and T8+ subsets may be independent of monocytes.

Antibodies, Monoclonal↗
PubMed1988

B cell hyperactivity and its relation to distinct clinical features and the degree of disease activity in patients with systemic lupus erythematosus.

Peripheral blood B cells that were actively proliferating, those actively secreting immunoglobulin, and those expressing an early activation marker, Ba antigens, on the surfaces were quantitated in 25 patients with systemic lupus erythematosus (SLE). B cell hyperactivity was found in almost all of the SLE patients, as demonstrated by any one of these measures of B cell activity. Moreover, we observed a strong positive correlation between the degree of disease activity and the amount of spontaneous incorporation of 3H-thymidine by B cells; the magnitude of the increase in frequency of spontaneous Ig-secreting cells in peripheral blood correlated strikingly with certain clinical features in these patients. Our findings suggest that there is heterogeneity of B cell hyperactivity in individual patients with SLE and, thus, that clinical subsets of SLE can be identified on the basis of B cell hyperactivity.

Adolescent↗
PubMed1988

[Periodontal surgical approach to the vertical fracture of the root. The application of composite resin to the fractured root surface].

For a patient who had a deep periodontal pocket without an attached gingiva on labial central area of left upper canine, a free gingival graft from the palate was done. After that, a flap was reflected and the cause of the lesion was determined. It became clear that there was a vertical fracture of the root and a composite resin (Clearfil-SC) was used to fill the fractured area. It has been 2 1/2 years since the therapy and the prognosis is good.

Gingiva↗
PubMed1987

Gangliosides of human melanoma: altered expression in vivo and in vitro.

The ganglioside composition of human melanoma was analyzed in five sets of tumor specimens obtained directly from surgery, from the autologous tissue culture cell lines, and from the autologous cell lines grown in athymic nude mice. Total gangliosides of these 15 melanoma specimens were isolated and purified, and the amount of each component ganglioside was analyzed by thin-layer chromatography and a thin-layer chromatography scanner. The ganglioside composition of the five surgical melanoma specimens clearly exhibited different patterns from each other. Moreover, none of the autologous cultured melanomas possessed the same ganglioside composition as their original biopsied tumors. However, when these melanoma cell lines were transplanted into nude mice, the ganglioside composition was converted back to the same ganglioside pattern as in the original surgical specimens. The results support the view that changes in the ganglioside composition of melanoma during in vitro growth are caused by the culture environment rather than by selection of melanoma cells with a particular genotype. Reestablishment of the original ganglioside patterns after passage in nude mice provides clear evidence that in vivo expression of gangliosides is a conserved and stable function specified by the human melanoma cells.

Animals↗
PubMed1987

Ultrastructural alterations of neuronal cells in a brain stem ganglioglioma.

A brain stem ganglioglioma in a 9-year-old female was examined ultrastructurally. The constituent neuronal (ganglion) cells displayed various ultrastructural features of neuronal degeneration including Hirano, Lafora and zebra bodies, inclusion-like aggregates of neurofilaments and large dilatations of rough endoplasmic reticulum. Although similar observations have been reported in peripheral neuronal tumors, this is the first reported occurrence in ganglioglioma, an uncommon tumor in the central nervous system. The coincidence of these alterations in the present tumor appeared to be of great interest, however, their exact etiology remained uncertain.

Brain Neoplasms↗
PubMed1987

Gangliosides of human melanoma.

The ganglioside composition of human malignant melanoma was studied with the use of 80 melanoma specimens, including 52 surgical specimens and 28 cultured cell lines. A ganglioside fraction was isolated and purified from each of these tissues, and the amount of each component ganglioside was assessed by thin-layer chromatography (TLC) and a TLC scanner. Five gangliosides (GM3, GD3, GM2, GD2, and alkali-labile ganglioside) were most commonly expressed by these melanomas. However, the total ganglioside amount (ranging from 33 to 302 micrograms/g wet wt of tissue) as well as the distribution of each ganglioside were widely heterogeneous in both biopsied and cultured melanomas. When the ganglioside expressions of cultured and biopsied melanomas were compared, GM2 and GD2 were minor components of biopsied melanomas but often became major components of cultured melanoma cells. Conversely, alkali-labile ganglioside was expressed more strongly on biopsied melanomas. This heterogeneity suggests that it will be necessary to analyze the ganglioside composition of biopsied melanomas before using monoclonal antibodies to melanoma-associated gangliosides for melanoma diagnosis or therapy.

Biopsy↗