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T Terashima

Publications and source records attributed to T Terashima.

At least 163 records · Page 9Linked to original sources
PubMed1989

Assessment of clinical application of test-dose concept for theophylline in patients with respiratory failure.

The possibility of clinical application of test-dose concept for theophylline was assessed in 11 patients with serious underlying disease who required theophylline. Based on the pharmacokinetic parameters calculated from the single intravenous aminophylline administration, they received a continuous infusion of aminophylline in order to maintain about 10 micrograms/ml plasma which is considered to be the lowest therapeutic level. Plasma levels during a constant rate infusion were assayed at 6:00, noon, 18:00 and midnight on the 3rd or 4th day after the infusion had started. There were no significant differences among plasma levels at each sampling time, but plasma levels varied ranging from 5.1 to 24.8 micrograms/ml (12.1 +/- 5.5 micrograms/ml: mean +/- S.D.), which values were in disagreement with the predicted value in some cases. The correlations of the theophylline clearance ratio to dose, pH, arterial partial pressures of oxygen (Pao2) and carbon dioxide (Paco2) ratios, which were calculated by dividing the value during continuous infusion by the value at the test dose, were investigated to evaluate which factor largely contributed to the failure of this dosing method. Although the clearance ratio did not correlate to pH and Pao2 ratios, significant negative relationships were observed between the clearance ratio and the dose (p less than 0.05) or Paco2 ratios (p less than 0.02). In other words, the alternation of dose or Paco2 resulted in the change of theophylline clearance. These findings suggest that the test-dose concept should not be used in the seriously ill patients whose theophylline clearance can change easily in relation to dose and/or Paco2 change.

Aged↗
PubMed1989

[Malignant pericardial effusion after esophageal cancer surgery successfully treated by intrapericardial instillation of CDDP].

The patient is a 63-year-old male with pericardial effusion who fell into cardiac tamponade one year and eight months following radical operation for esophageal cancer (stage IV). Because the cytological diagnosis for the punctured specimen of pericardial effusion indicated class V, we recognized it as pericardial recurrence and instilled CDDP 20 mg and 25 mg into the pericardial cavity. The pericardial effusion disappeared completely. There are many reports of CDDP instillation into the pleural cavity or the peritoneal cavity, but few into the pericardial cavity. For esophageal cancer, this is the first report in Japan. The patient is still alive at present after fourteen months have passed. It is considered that this method is effective for malignant pericardial effusion due to advanced esophageal cancer.

Carcinoma, Squamous Cell↗
PubMed1988

Immunohistochemical detection of GTP-binding regulatory protein (Go) in the autonomic nervous system including the enteric nervous system, superior cervical ganglion and adrenal medulla.

The localization of a GTP-binding regulatory protein, Go, in the autonomic nervous system including the enteric nervous system, superior cervical ganglion, and adrenal medulla, has been immunohistochemically examined by use of affinity-purified antibody against the alpha-subunit of Go. In the small intestine, dense Go-immunoreactive products were localized on the enteric nervous system, i.e. the myenteric plexus of Auerbach and the submucosal plexus of Meissner. In the superior cervical ganglion, presynaptic terminals were strongly immunoreactive to the Go antibody. The adrenal medulla was stained with this antibody, but the adrenal cortex was not immunoreactive to this antibody. Thus, the present study strongly suggests that Go is localized in the autonomic nervous system and plays its role in transmembrane signal transmission in this system.

Adrenal Medulla↗
PubMed1988

Immunohistochemical analysis of the localization of guanine nucleotide-binding protein in the mouse brain.

A guanine nucleotide-binding protein, G0, is a heterotrimer with the alpha- and beta gamma-subunits (referred to here as alpha 0 and beta gamma, respectively). We examined the distribution pattern of the anti-alpha 0 and anti-beta gamma immunoreactive products in the hippocampus, and cerebral and cerebellar cortices of the mouse brain. In the hippocampus, alpha 0- and beta gamma-immunoreactivities were localized in the neuropil of the stratum oriens, stratum radiatum and stratum lacunosum-moleculare, but were absent from the cell bodies of the pyramidal cells and their apical dendritic shafts. In the cerebral cortex, alpha 0- and beta gamma-immunoreactivities were seen in the neuropil of all 6 layers of the cerebral cortex, especially in the uppermost molecular layer (layer I), and were absent from cell bodies of neurons and their apical dendritic shafts. In the cerebellar cortex, the molecular layer was heavily stained with anti-alpha 0 and beta gamma-antibodies. The present study revealed that the distribution pattern of beta gamma-immunoreactivities in these structures of the mouse brain was strikingly similar to that of the alpha 0-immunoreactivities.

Animals↗
PubMed1988

Cortical axons branch to multiple subcortical targets by interstitial axon budding: implications for target recognition and "waiting periods".

We are studying how axons branch in vivo. Individual cortical neurons send axons to both the spinal cord and the basilar pons. Here we show that the corticopontine projection develops by an interstitial budding of collaterals from parent axons rather than a reported mechanism of axon branching, growth cone bifurcation. This mechanism is used regardless of whether the parent axon's postpontine segment, which forms the corticospinal projection, is permanent (motor cortex) or transient (visual cortex). Budding occurs days after the parent axons grow spinally past the pons, accounting for the "waiting period" reported in this system in contrast to an alternative explanation that the growth cones pause outside of their target. Timing and location of pontine collateral budding vary with cortical origin of the parent axon and are correlated with the temporal ordering of axon arrival.

Animals↗
PubMed1987

Light microscopy of GTP-binding protein (Go) immunoreactivity within the retina of different vertebrates.

To examine species differences in the distribution pattern of guanosine triphosphate (GTP)-binding protein (Go) within the vertebrate retina, paraffin-embedded retinae from a number of vertebrate species, including the goldfish, frog, turtle, chicken, monkey, and human, were immunohistochemically stained with affinity-purified antibody against the alpha-subunit of Go. Go-immunoreactive products were found to be located in the neuropil, but not in the cell bodies of neurons, in the retina of all these species. However, some species differences were observed. In the frog, monkey and human, the inner plexiform layer (IPL) was homogeneously stained with this antibody, but in the goldfish, turtle and chicken, the IPL was heterogeneously stained. In the frog, chicken, turtle and human, the outer plexiform layer (OPL) was densely stained with this antibody, but in the goldfish and monkey, the OPL was rather faintly immunoreactive to the antibody. In the goldfish, monkey and human, the outer nuclear layer (ONL) was not immunoreactive to the Go-antibody, whereas in the frog, turtle and chicken, the ONL was immunoreactive to it. The implications of these species differences in Go localization in the vertebrate retina are discussed.

Animals↗
PubMed1987

Endocrine cells in pancreatic islets of Langerhans are immunoreactive to antibody against guanine nucleotide-binding protein (Go) purified from rat brain.

Guanine nucleotide-binding proteins (G proteins) are usually classified into four subclasses (Gs, Gi, Go and Gt or transducin). We localized the anti-Go immunoreactivities in islets of Langerhans of the rat pancreas by using affinity-purified antibody against the alpha-subunit of Go purified from rat brain. Endocrine cells of the islets of Langerhans were stained with this Go antibody, but the acinar cells in the exocrine portion of the pancreas were immunonegative to this antibody. These findings strongly suggest that Go protein functions as intermediaries in the transmembrane signalling pathway in the endocrine cells of the islets of Langerhans.

Adenylyl Cyclases↗
PubMed1987

Immunohistochemical localization of guanine nucleotide-binding protein in rat retina.

The localization of a guanine nucleotide-binding protein, Go, in rat retina has been immunohistochemically examined by use of affinity-purified antibody against the alpha-subunit of Go. Dense Go-immunoreactive products are localized in the inner and outer plexiform layers which are considered as the region of synaptic interplay between neuronal components of the retina. Weak Go-immunoreactivity is also found in the neuropil of the ganglion cell layer and the inner nuclear layer. The selective enrichment of Go in synaptic zones of the retina suggests its major role in neuronal transmission within the retina.

Animals↗
PubMed1987

Thalamic connectivity of the primary motor cortex of normal and reeler mutant mice.

Reeler, an autosomal recessive mutation in mice, causes cytoarchitectonic abnormalities of the cerebral cortex, which are characterized by malposition of neurons. Retrograde and anterograde transport of horseradish peroxidase (HRP) was employed to examine the reciprocal connectivity between the hindlimb area of the primary motor cortex (MI) and thalamus of normal and reeler mutant mice. In the normal mouse, most of the cells labelled after HRP injection into the hindlimb area of MI were located in the ventrolateral nucleus, the lateral division of the ventrobasal nucleus, the central lateral, paracentral and central intralaminar nuclei, and the medial division of the posterior complex. HRP reaction product anterogradely transported was also observed in the same nuclei and in the thalamic reticular nucleus. In the reeler mutant mouse, retrogradely labelled neurons and anterogradely labelled terminals were again found in the nuclei referred to above, and the distribution pattern and morphology of HRP-filled neurons were also similar to those of normal controls. The present results suggest therefore that the normal reciprocal connectivity between MI (hindlimb representation) and thalamus is preserved in the reeler mouse. That is to say, dislocated cortical neurons appropriately project to their target nuclei of the thalamus, and conversely, thalamic neurons send their axons precisely to their target cortical areas of the radially disorganized cortex.

Animals↗
PubMed1987

The pathways responsible for the characteristic head posture produced by lesions of the interstitial nucleus of Cajal in the cat.

(1) Experiments were performed in cats to examine effects of lesion of the interstitial nucleus of Cajal (INC) on head posture and the responsible pathway. Unilateral INC lesions resulted in lateral tilt of the head to the opposite side, and bilateral INC lesions resulted in dorsiflexion of the head as reported earlier. Such characteristic head posture was produced by successful kainic acid injections as well as by electrolytic lesions, suggesting that it was not due to damage of nerve fibers passing through the INC, but was produced most probably by damage of nerve cells in the INC. Electromyographic (EMG) recordings in unilateral INC-lesioned cats showed that activity was higher in the ipsilateral than in the contralateral major dorsal neck muscles (biventer, splenius, complexus, and rectus), and also higher in the contralateral than in the ipsilateral obliquus capitis caudalis muscle. The pattern of EMG activity was basically similar either when the cats presented typical head tilt or when their head was fixed to the frame at the stereotaxic plane. Characteristic head posture resulting from INC lesions seems consistent with the head posture produced by activation of these muscles. (2) Interruption of the medial and lateral vestibulospinal tracts did not significantly influence head tilt that had been produced by INC lesions. Characteristic head tilt was produced by INC lesions after cats had received bilateral labyrinthectomies, bilateral lesions of most of the vestibular nuclei, and bilateral aspiration of the cerebellar vermis and most of the lateral vestibular nuclei, indicating that typical head tilt can be produced without the vestibular nuclei and cerebellar vermis. (3) The medial longitudinal fasciculus (MLF) was interrupted at different levels to cut the major descending fibers from the INC. MLF interruption at the caudal midbrain produced typical head tilt, although MLF cut at the caudal pons and medulla was ineffective. Bilateral parasagittal cuts lateral to the MLF in the pons produced severe dorsiflexion of the head, and a subsequent unilateral INC lesion produced no further head tilt. These results suggest that fibers originating in the INC, removal of which is responsible for the typical head tilt, run through the MLF in the midbrain, and leave it in the pontine level.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗