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Biomedical subjects

T Tamura

Publications and source records attributed to T Tamura.

At least 1,225 records · Page 68Linked to original sources
PubMed1984

Heterophile antibodies in sera of patients with Chediak-Higashi syndrome.

Sera of 6 patients with Chediak-Higashi syndrome and sera of their mothers were studied for heterophile antibodies. Sera of 5 patients as well as 5 sera of their mothers contained antibodies against trypsinized bovine erythrocytes, tissue sediments of guinea pig kidney or high molecular weight glycoprotein (HMWGP) of bovine erythrocyte stromata. The antibodies combining with HMWGP in enzyme immunoassay belonged to IgM and IgG classes. Although none of the sera had significant titer of agglutinins against sheep erythrocytes, on the basis of absorption and inhibition studies, these antibodies seemed to belong to the Hanganutziu-Deicher group of antibodies.

Animals↗
PubMed1984

High level of plasma inactive renin in Bartter's syndrome.

In 10 cases of Bartter's syndrome, plasma active and inactive renin (AR and IR) were measured by two different methods. First, plasma renin activity (PRA), and total renin activity (TRA) after activating IR with trypsin, were measured by radioimmunoassay (RIA) of angiotensin I (AI) generated from endogenous substrate. And secondly, plasma active renin concentration (ARC) and total renin concentration (TRC) were measured by RIA of AI generated in the presence of an excess of exogenous substrate. The difference between TRA and PRA, and between TRC and ARC were designated as inactive renin activity (IRA) and inactive renin concentration (IRC), respectively. Small amounts of IRA were found only in 2 cases and no IRA in 8 cases. However, the existence of large amounts of IR in Bartter's syndrome was revealed by the IRC determination. This suggests that the shortage of endogenous renin substrate, consumed by the markedly increased AR, may have interfered with the detection of IRA in Bartter's syndrome, though the IR is markedly increased as well. The molecular weights of AR and IR were determined by Sephadex G-100 gel filtration in 3 cases. Both AR and IR seemed to be smaller than those of normal subjects.

Adolescent↗
PubMed1984

Nationwide survey of cases of choledochal cyst. Analysis of coexistent anomalies, complications and surgical treatment in 645 cases.

A nationwide survey of cases of choledochal cyst was conducted to obtain the data on cases of choledochal cyst in Japan between 1972 and 1982. A total of 645 cases in 73 institutions, including 52 cases from this hospital, were surveyed. Choledochal cyst was associated with an anomalous arrangement of the pancreaticobiliary ductal system in 90.2% of the cases which were examined extensively. The serum amylase level was elevated in 26.6% of the cases. Calculus disease of the biliary duct was recognized in 28.5% of the cases. Biliary carcinoma developed in 54 (8.4%) of 645 cases. The incidences of biliary carcinoma were 0.3% (1/306) in cases in children and 15.6% (53/339) in adult cases; carcinoma was found in the wall of the choledochus in 30, and in the gallbladder in 16 of these 54 cases. The nationwide survey showed that an anomalous arrangement of the pancreaticobiliary ductal system was closely associated with choledochal cyst and that cases of biliary carcinoma developed with the advance of age. Therefore, early resection of the cyst wall and reconstruction of the biliary duct is proposed as suitable surgical treatment for stopping reflux of pancreatic juice into the biliary duct.

Adolescent↗
PubMed1984

Studies on Paul-Bunnell (P-B) antigen-antibody system. V. Immunoglobulin classes of P-B antibodies.

By means of enzyme immunoassay (EIA) with purified Paul-Bunnell (P-B) antigen of bovine erythrocytes and sera of infectious mononucleosis, P-B antibodies of IgG (92%), IgM (94%), IgA (77%), and IgE (64%) classes were demonstrated. Absorption and inhibition studies ascertained the P-B specificity of the antibodies of all four classes. Absorption of selected IM sera with sheep erythrocytes revealed the presence of both BS and B antibodies of P-B specificity in these sera. In three IM patients whose sequential serum samples were studied, P-B antibodies of all four classes appeared and reached their peaks within a few weeks after the onset of the disease, persisted for a month, and sharply declined thereafter.

Antibodies↗
PubMed1984

Immunological studies on Kawasaki disease. I. Appearance of Hanganutziu-Deicher antibodies.

Sera of patients with Kawasaki disease were studied for heterophile antibodies by means of enzyme immunoassay (EIA) with enzyme conjugated antisera to human IgM, IgG, IgA and IgE. Antibodies of IgM (43%), IgG (3%), IgA (11%) and IgE (49%) classes were demonstrated that combined with high molecular weight glycoprotein (HMWGP) of bovine red blood cells (BRBC) one of the antigenic preparations of the Hanganutziu-Deicher (H-D) heterophile system. Studies on sequential sera of the patients revealed that HMWGP antibodies of IgM and IgE classes began to appear in the second week, reached their peaks in the third week of the disease and declined gradually thereafter. Absorption studies on the positive sera showed that the HMWGP antibody activities were abolished by BRBC, sheep red blood cells and guinea-pig kidney tissues, confirming H-D specificity of these antibodies. EIA inhibition studies showed that the antibody activity was inhibited by HMWGP and partially by asialo-HMWGP and NGNA ganglioside rich preparation of BRBC but not by purified Paul-Bunnell or Forssman antigens. These results indicate that the H-D antibodies under investigation consist of antibodies of two different specificities; one directed against asialo-HMWGP and the other NGNA ganglioside of BRBC. Circulating immune complexes (IC) were demonstrated in 23% of the patients by means of anti-antibody inhibition test. Evidence was presented that IC in the sera of five patients were composed of H-D (HMWGP) antigen and its corresponding antibodies.

Antibodies, Heterophile↗
PubMed1984

Mixed agglutination with guinea pig tissue sediments for detection of heterophile antibodies.

Mixed agglutination (MA) test with sediments of guinea pig kidney (GPK) homogenates and indicator red blood cells of bovine (BRBC) or sheep (SRBC) origin was established for detection of human heterophile antibodies. By means of MA test with BRBC indicator cells, heterophile antibodies of Hanganutziu-Deicher (H-D) specificity were demonstrated in sera of patients with syphilis (20%), lepromatous leprosy (57%), infectious mononucleosis (45%), Chediak-Higashi syndrome (73%), Kawasaki disease (58%), multiple sclerosis (58%), and leukemias (13%), as well as in sera of subjects who received injections of foreign species sera (20%). Some but not all BRBC-positive sera gave positive MA tests when SRBC were employed as indicator cells. None of 13 multiple myeloma sera tested gave positive results. The incidence of positive reactions in normal human sera was 3%. Neutralization of H-D antibodies in representative pathologic sera by purified heterophile antigens showed that the antibodies under investigation were mostly directed against antigen(s) of high molecular weight glycoprotein, but not N-glycolyl-neuraminic acid (NGNA) ganglioside fraction of BRBC.

Agglutination Tests↗
PubMed1984

Paul-Bunnell antigen in normal human tissues.

The BS antigen, the component of the Paul-Bunnell (P-B) antigen complex that is shared by bovine (BRBC) and sheep red blood cells (SRBC), was demonstrated in chloroform-methanol extracts from peripheral blood leukocytes of 4 of 26 healthy young individuals. One of the extracts formed a precipitation line with infectious mononucleosis (IM) sera, which merged into a reaction of identity with the corresponding line formed by the purified P-B antigen of BRBC. The BS antigen of the P-B complex was also demonstrated in tissue sediments of an apparently normal human kidney by means of mixed agglutination test with IM sera and trypsinized BRBC as the indicator. Results of this study suggest that the BS antigen is expressed in normal tissues of some individuals as an intracellular antigen, whereas in various pathologic conditions it is expressed as cell-surface antigen and/or appears in a soluble form in the patient's circulation.

Adult↗