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Biomedical subjects

T Takeuchi

Publications and source records attributed to T Takeuchi.

At least 1,405 records · Page 78Linked to original sources
PubMed1988

Immunocytochemical and ultrastructural characterization of human T-lymphotropic virus type I (HTLV-I)-producing rabbit lymphoid cell lines.

Fine structural and immunocytochemical characterization of rabbit lymphoid cell lines transformed by human T-lymphotropic virus type I (HTLV-I) was carried out. All nine cell lines tested were reactive with anti-HTLV-I-positive human, monkey, and rabbit sera and monoclonal antibody to HTLV-Ip 19, but not with anti-HTLV-I-negative sera and monoclonal antibodies to human Ia and pan-T antigens. All cell lines were strongly positive for monoclonal antibodies to rabbit Ia and pan-T antigens. Ultrastructurally, each cell line contained C-type virus particles in varying numbers in the extracellular space. These particles showed replication patterns similar to those in HTLV-I or simian T-lymphotropic virus type I (STLV-I)-producing human or monkey cells. In addition, anti-HTLV-I-positive rabbit serum gave positive immunoreactivity to HTLV-I or STLV-I by indirect immunoferritin method. These results indicate that the ultramorphology and replication patterns of HTLV-I in rabbit cell lines are indistinguishable from those of HTLV-I in human and monkey cell lines, HTLV-I in rabbit cells shares the common surface antigenic determinants with HTLV-I or STLV-I in human or monkey cells, and that these cells are definitely rabbit T cells bearing their own Ia antigens.

Animals↗
PubMed1988

Measurement of portal blood flow by an ultrasonic "duplex" system composed of a pulsed Doppler flowmeter and a linear-type real time B mode electroscanner.

The portal vein hemodynamics of patients with various liver diseases were investigated by means of a duplex system consisting of a linear electroscanner and a pulsed Doppler flowmeter. In cases of chronic liver disease, the cross-sectional area of the portal vein trunk became greater as the liver injury proceeded, while the maximum velocity tended to decrease. However, blood flow volume was no different between the controls and the chronic liver disease group. On the other hand, the splenic venous flow volume tended to increase as the liver injury advanced, suggesting that the increase in splenic venous flow volume is closely related to the formation of esophageal varices. This method permits non-invasive observation of changes during the course of various types of liver disease.

Blood Flow Velocity↗
PubMed1988

CD11 molecule defines two types of suppressor cells within the T8+ population.

It has been shown that T8+ cells are comprised of functionally heterogeneous subpopulations such as suppressor, cytotoxic, and NK cells. In this report, we attempted to delineate the functional heterogeneity of T8 cells defined by anti-CD11 antibody (anti-Mol). Although allospecific cytotoxic activity was restricted to the T8+Mol- subset, suppression of PWM IgG synthesis could be elicited in both the T8+Mol+ and the T8+Mol- subset of cells. However, the mechanism of suppression was different in these two subsets. Suppression by the T8+Mol- subset of cells required the interaction with the T4+2H4+ suppressor inducer cells, whereas the T8+Mol+ subset of cells could suppress in the absence of the suppressor inducer cells. Moreover, recombinant interleukin-2 alone could augment this suppression by the T8+Mol+ subset, but did not induce suppression by the T8+Mol- subset. In contrast, NK and LAK activity was exclusively found in the T8+Mol+ subset of cells but not in the T8+Mol- subset of cells. These results suggest that the CD11 molecule is useful for distinguishing novel subsets of T8 cells.

Antibodies, Monoclonal↗
PubMed1988

Studies on the nature of thiamine pyrophosphate binding and dependency on divalent cations of transketolase from human erythrocytes.

1. The binding kinetics for [35S]thiamine pyrophosphate to transketolase and the dependency of transketolase on divalent cations for activity were investigated. 2. With Scatchard analysis, dissociation constant (Kd) and n value were calculated to be 0.2 x 10(-6) M and 0.66 respectively. 3. The activity of the reconstituted enzyme increased in the order of Co2+ less than Mn2+ less than Ca2+ less than Mg2+. The native transketolase contained Mg2+ in its molecular structure.

Binding Sites↗
PubMed1988

Primary pulmonary hypertension in pregnancy.

Primary pulmonary hypertension (PPH) is an uncommon but serious disease. Most patients with PPH are young women and the disease is more serious and eventful in pregnant women. We have experienced a patient with PPH in pregnancy, who was delivered successfully but died suddenly on the 7th day after the delivery. We report the obstetric course and the clinical management for the delivery of the patient with PPH.

Adult↗
PubMed1988

The expression of melanosomal matrix protein in the transdifferentiation of pigmented epithelial cells into lens cells.

A monoclonal antibody (MC/1) was constructed against melanosomes purified from the chicken pigmented epithelial cells (PECs) in order to characterize the differentiative phenotypes of PEC in the process of transdifferentiation into lens cells. Immunofluorescent studies revealed that MC/1 antibody specifically stains both retinal PECs in the eye and melanocytes in the skin, of chicken embryos. Immunoelectron microscopy showed that the antigen molecules are located on the peripheral region of the melanosomal matrix. A single protein band with an apparent molecular weight of 115,000 was labelled by MC/1 in Western blotting. The 115 kDa polypeptide identified by MC/1 is considered to be a member of the melanosomal matrix proteins. The maintenance of specificity of pigment cell nature is followed in the system of transdifferentiation of PEC into lens in vitro, utilizing 115 kDa protein as a marker. In the dedifferentiated PECs, this protein was undetectable.

Animals↗
PubMed1988

Trisomy 4 in a case of acute nonlymphocytic leukemia (M2).

Trisomy 4 in a Japanese patient with type M2 acute nonlymphocytic leukemia is reported. Karyotypic analysis was unsuccessful at diagnosis and complete remission lasted 4 years. The cytogenetic change was detected in the first and second relapses as the only chromosome abnormality. Unusual nuclear irregularities were found in many leukemic cells.

Acute Disease↗
PubMed1988

Somatomedin-C in the kidney of streptozotocin diabetic rats.

To evaluate the possible role of somatomedin-C, insulin-like growth factor I, in renal hypertrophy in early diabetes, kidney tissue SmC concentrations were measured in streptozotocin-induced (80 mg/kg ip) diabetic rats. Body weight, liver weight, plasma SmC concentration, and SmC concentration in the liver of diabetic rats were significantly lower than those of controls. Seven days after induction of diabetes, the kidney weight (898 +/- 95 mg) in diabetic rats was significantly greater than that in controls (755 +/- 69 mg), while SmC concentration in the kidney of diabetic rats (1.7 +/- 0.3 U/g kidney) was significantly lower than that of control rats (5.4 +/- 0.6 U/g kidney). These results suggest that renal SmC may not have an important role in renal hypertrophy in early stages of diabetes and that renal production of SmC may be impaired by insulin deficiency in rats.

Animals↗
PubMed1988

The effect of maturation on insulin secretion after starvation in isolated perfused rat pancreas.

To determine the effect of maturation on glucose-induced insulin release after starvation, we investigated the insulin release from perfused pancreas of young adult rats after fasting for 18-20 hr. In the rats weighing 300-400 g (12-14 w) and 200-250 g (7-8 w), the glucose-induced insulin secretion from perfused pancreas of starved rats was similar to that of fed rats. While, in the rats weighing 100-150 g (4-5 w), both the first and the second phase of glucose-induced insulin secretion from perfused pancreas of starved rats was markedly decreased compared with that of fed rats. These results suggest that insulin response to glucose is not matured in young below 150 g (5 w).

Aging↗
PubMed1988

Evaluation of the cytotoxicity of sodium valproate on primary cultured rat hepatocytes.

Primary cultured rat hepatocytes were used to study the cytotoxicity of sodium valproate (VPA). Cytotoxicity was monitored by measurement of leakage of intracellular enzymes into the culture medium: lactate dehydrogenase (LDH), glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT). The effects of D,L-carnitine and albumin administration on the cytotoxicity were evaluated. LDH leakage rose with an increasing dose of VPA. Administrations of D,L-carnitine and albumin reduced VPA hepatotoxicity. Our data suggest that VPA-induced hepatotoxicity is dose-related and may be modulated by serum carnitine and albumin levels.

Alanine Transaminase↗
PubMed1988

IgA deposition in alcoholic liver disease. An immunoelectron microscopic study.

The ultrastructural localization of IgA, IgA1, and IgA2 deposited on liver tissues from 13 patients with alcoholic liver disease and 9 patients with nonalcoholic liver diseases was investigated by the immunoperoxidase method. A continuous pattern of IgA deposition along the perisinusoidal area was observed not only in the alcoholic patients but also in the nonalcoholic patients. Ultrastructurally, IgA deposits were seen on the plasma membranes of Kupffer cells, endothelial cells, and hepatocytes and in the endocytotic vesicles and phagosomes of Kupffer cells and endothelial cells. In the alcoholic group, deposits of IgA on collagen fibers were observed at sites where pericellular fibrosis developed but, on the other hand, IgA deposits were diminished or absent on the plasma membranes of hepatocytes. IgA1 showed the same deposit pattern as IgA. IgA2 was observed on and in Kupffer cells and endothelial cells but was not detected on the plasma membranes of hepatocytes. From these studies, the authors conclude that IgA deposition in the liver is not specific for alcoholic liver disease but may reflect the reduced metabolism of damaged liver. An additional finding was that, of the IgA subclasses, only IgA1 combined with the plasma membranes of hepatocytes.

Hepatitis↗
PubMed1988

Experimental and clinical studies on epidermal growth factor for gastric mucosal protection and healing of gastric ulcers.

In experimental studies, 0.6 N HCl-induced gastric mucosal injury was significantly severe in submandibularectomized rats (SMR rats) than that in either SMR rats receiving exogenous mouse EGF (SMR + EGF rats) or controls. This was also true in gastric injury induced by 0.4 N HCl under pretreatment with indomethacin to reduce gastric mucosal prostaglandins (PGs). Somatostatin (SLI), PGE2, and PAS-stained mucus in the corpus were significantly reduced in SMR rats in comparison to SMR + EGF and control rats. In clinical studies, salivary EGF secretion was much higher in peptic ulcer patients than healthy controls. beta-Urogastrone was effective in the treatment of gastric ulcers. On the basis of experimental studies, we conclude that the protective effect of EGF on the gastric mucosa is, in part, mediated indirectly by increases in SLI, PGE2, and mucus production. However, endogenous, as well as exogenous, EGF has an important direct, cytoprotective effect on the gastric mucosa. From the clinical studies, we also conclude that salivary EGF secretion in ulcer patients increases in a homeostatic response to the presence of an ulcer, facilitating ulcer healing. Furthermore, we believe that beta-urogastrone, human EGF, might prove to be an effective drug in the clinical treatment of gastric ulcers.

Animals↗
PubMed1988

Neonatal hemophilia B with intracranial hemorrhage. Case report.

It is uncommon for infants with hemophilia to have excessive bleeding during the neonatal period. Even if bleeding occurs, it rarely becomes life-threatening, such as in intracranial hemorrhage (ICH). We here report a case of a 4-day-old boy who had intracranial hemorrhage as the first complication of hemophilia B. Computerized axial tomography (CT scan) and ultrasonography were very useful for early diagnosis. Only a few cases of neonatal hemophilia with intracranial hemorrhage have been reported, but the occurrence of this complication is probably more frequent. We reviewed seven cases (including our case) with intracranial hemorrhage as the first manifestation of neonatal hemophilia. Although these infants showed good prognosis as to survival, permanent residual neurological deficits remained in all of them. It is emphasized that intracranial hemorrhage due to hemophilia may occur in neonates even without a family history. Urgent neuroimaging and coagulation studies are necessary for an early and adequate diagnosis.

Cerebral Hemorrhage↗
PubMed1988

A monoclonal antibody that recognizes a common antigen in myeloid and human T-cell leukemia virus type I-carrying cells.

A novel monoclonal antibody, designated D-213, was generated to human myeloid cell line PL-21. D-213 defined a 47-kD antigen which was specifically expressed in normal and leukemic cells of the myeloid lineage and in T-cell lines carrying human T-cell leukemia virus type I. This antibody was also reactive with adult T-cell leukemia/lymphoma (ATLL) cells in formalin-fixed, paraffin-embedded tissues. D-213 would be useful for making an immunohistochemical diagnosis of ATLL in cases where serological or virological information is not available.

Animals↗
PubMed1988

Suppression of in vitro production of anti-U1-ribonucleoprotein antibody by monoclonal anti-idiotypic antibody to anti-U1-ribonucleoprotein antibody.

We produced monoclonal anti-idiotypic antibody against immunoaffinity purified anti-U1-ribonucleoprotein (RNP) antibody from a patient (K.T.), by the cell fusion procedure. The specificity of monoclonal anti-idiotypic antibody (IgG1, kappa) was determined by inhibition studies. With the monoclonal anti-idiotypic antibody, cross-reactive idiotypes on anti-U1-RNP antibodies from unrelated patients with anti-U1-RNP antibody was detected in 57% of the samples. The anti-idiotypic antibody specifically suppressed the in vitro production of anti-U1-RNP antibody by lymphocytes from the patient K.T., and unrelated patients with a cross-reactive idiotype, in whom idiotype-reactive T cells were demonstrated. The results indicate that anti-idiotypic antibody may modulate the regulation of in vitro anti-U1-RNP antibody production.

Animals↗