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Biomedical subjects

T Takeda

Publications and source records attributed to T Takeda.

At least 1,171 records · Page 65Linked to original sources
PubMed1985

Chronic food restriction modulates the advance of senescence in the senescence accelerated mouse (SAM).

The effects of chronic food restriction on grading scores of senescence, deposition of senile amyloid (ASSAM), mean life span and 10th decile were investigated by using animal models for accelerated senescence (SAM-P/1) and for normal aging (SAM-R/1). The experimental groups consisted of control (ad libitum fed), 80% (fed 80% of control intake), and 60% (fed 60% of control intake) groups. The grading score of SAM-P/1 mice was significantly improved in the 60% group, but not in the 80% group, compared to the control group. The grading score of SAM-R/1 mice, however, was significantly less than that in the control group in both the 60 and 80% groups. In SAM-P/1 mice liver, skin and testis, the severity of senile amyloid deposition was significantly less with 40% food restriction (60% group) than in the control group. A restriction of 20% (80% group) had no influence on amyloid deposition. A definite tendency to prolong mean life span (24.3%) and 10th decile (65.9%, mean life span of the last 10th of survivors of a group) was observed in the 60% group of SAM-P/1 mice, but the changes were not statistically significant. In the 80% group of SAM-P/1 mice and also in either restriction group of SAM-R/1 mice, however, such a tendency was not evident. These results indicate that 40% food restriction modulates the advance of senescence in these mice.

Aging↗
PubMed1985

A competitive immunosorbent assay for the detection of heat-stable enterotoxin of Escherichia coli.

A competitive ELISA procedure for the detection of Escherichia coli heat stable enterotoxin (ST) with monoclonal antibody has been developed. This test is 10 times more sensitive than the suckling-mouse bioassay and it is specific, simple and cheap. A set of 882 strains of E. coli isolated from man were tested both by ST-ELISA and suckling-mouse bioassay, the latter serving as the reference method. Positive results in both tests were obtained with 152 strains. The remaining strains gave negative results in both tests, with the exception of two strains, known to be ST producers, that gave negative results in the suckling-mouse assay, but gave positive results by the ELISA method.

Animals↗
PubMed1985

Uptake of intravenously administered soluble immune complexes by type I alveolar cells and macrophages in mice.

Mice (C57BL/6, n = 5) were given a single intravenous injection of soluble bovine serum albumin (BSA)-rabbit anti-BSA immune-complexes (IC) prepared at five-fold antigen excess. The mice were sacrificed 15 and 30 min, and 1, 2, 4 and 12 h after injection. Granular immunofluorescence for BSA and rabbit IgG, in a pattern consistent with IC aggregation, was observed in the alveolar walls and in the cytoplasm of alveolar cells. Moderate immunofluorescence was observed 15 min after IC injection and maximal immunofluorescence was observed at 30-60 min which decreased rapidly 2 h after IC administration. Only a trace amount of immunofluorescence was observed at 4 h and none was seen at 12 h. Light microscopic immunoperoxidase staining showed localization of IC in the interstitium of the alveolar septa and in type I alveolar cells and alveolar macrophages. By immunoelectronmicroscopy, the uptake of IC in the cytoplasm of type I alveolar cells and alveolar macrophages was confirmed.

Animals↗
PubMed1985

Pseudo-coarctation of the abdominal aorta associated with renovascular hypertension.

A case of pseudo-coarctation of the abdominal aorta associated with renovascular hypertension has been reported. The abdominal aorta of the patient was kinked at the level of L1-L2 without a pressure gradient, which was consistent with pseudo-coarctation of aorta. Both renal arteries arose from the aorta at the level of lower T12 and had severe multiple stenoses. We have discussed the possible etiology of the developmental abnormalities of the arterial system in this patient.

Adult↗
PubMed1985

Significance of the measurement of urinary alanine aminopeptidase and N-acetyl-beta-D-glucosaminidase activity in evaluating patients with essential hypertension.

To examine the relationship between the urinary levels of alanine aminopeptidase (AAP) or N-acetyl-beta-D-glucosaminidase (NAG) and the advance of essential hypertension, we measured the urinary levels of these enzymes in 20 normotensive controls, 8 subjects with borderline hypertension and 40 subjects with WHO stage I and stage II essential hypertension. The urinary level of NAG in stage II hypertensives was higher than that in the normotensives, and borderline or stage I hypertensives (p less than 0.01). Systolic blood pressure and the urinary level of NAG was positively correlated in hypertensives (rs = 0.43, p less than 0.01). The urinary level of NAG was correlated inversely with renal blood flow (rs = -0.61, p less than 0.01). The urinary level of AAP in stage II hypertensives was also higher than that in the normotensives (p less than 0.01) or stage I hypertension (p less than 0.01), but the urinary AAP level was not significantly correlated with systolic blood pressure or renal blood flow in hypertension.

Acetylglucosaminidase↗
PubMed1985

[Cardiovascular hemodynamics in patients with essential hypertension during multi-stage exercise].

We investigated cardiovascular hemodynamics in patients with essential hypertension at rest and during exercise. Eight patients with borderline hypertension (Group B), 18 patients with sustained hypertension, and 10 normotensive subjects (Group N) were studied. Patients with sustained hypertension were categorized in two groups; 10 patients without cardiac hypertrophy (Group NH), and eight patients with cardiac hypertrophy (Group HH). To assess the cardiovascular hemodynamics, M-mode echocardiograms, electrocardiograms and carotid pulses were recorded during multistage exercise using a supine bicycle ergometer. Blood pressure was measured simultaneously by the cuff method. At rest, isovolumic relaxation times (IRT) in Groups NH and HH were significantly prolonged as compared with that of Group N. The IRT of Group HH was significantly prolonged as compared with that of Group NH (Group N: 0.06 +/- 0.01 sec, Group B: 0.07 +/- 0.01 sec, Group NH: 0.08 +/- 0.02 sec, Group HH: 0.09 +/- 0.01 sec, respectively). Only the atrial filling fraction of Group HH was significantly increased over that of Group N (Group N: 25.6 +/- 12.1%, Group B; 30.0 +/- 8.1%, Group NH: 27.9 +/- 11.3%, and Group HH: 41.4 +/- 13.1%, respectively). The total peripheral vascular resistance (TPR) in Groups NH and HH was significantly increased over that of Group N (Group N: 1600 +/- 224 dyne/sec/cm-5, Group B: 1704 +/- 465 dyne/sec/cm-5, Group NH: 2370 +/- 608 dyne/sec/cm-5, and Group HH: 3290 +/- 1598 dyne/sec/cm-5, respectively). There was no significant difference in the cardiac index, ejection fraction or heart rate among all groups at rest and during exercise. However, the difference between TPR at rest and TPR at a load of 75 watt (delta TPR) was increased in Groups NH and Group HH over Group N (Group N: 558 +/- 166 dyne/sec/cm-5, Group B: 614 +/- 336 dyne/sec/cm-5, Group NH: 883 +/- 415 dyne/sec/cm-5, and Group HH: 1306 +/- 774 dyne/sec/cm-5, respectively). From these findings, we concluded that there is early diastolic left ventricular dysfunction in Groups NH and HH, and that the degree is more distinct in Group HH. It seems that systolic left ventricular function of both groups is normal during moderate exercise loads, and marked reduction of the TPR in both groups during exercise may be caused by marked peripheral vascular dilatation.

Adult↗
PubMed1985

[Left ventricular function in essential hypertension during isoproterenol infusion].

The left ventricular function of 23 patients with essential hypertension was investigated during infusion of isoproterenol (ISP). These patients consisted of 13 without cardiac hypertrophy (Group NH) and 10 with cardiac hypertrophy (Group HH). Ten normotensive subjects served as normal controls. To assess left ventricular functions, M-mode echocardiograms were recorded at rest and after ISP infusion for 5 minutes (0.005 and 0.01 microgram/kg/min). There were no significant differences in peak negative dD/dt in all groups at rest. But peak negative dD/dt of Group HH significantly decreased after an infusion of 0.005 microgram/kg/min ISP (Group N: 3.43 +/- 0.69, Group NH: 3.15 +/- 0.61, and Group HH: 2.49 +/- 0.48 cm/sec, respectively). The peak negative dD/dt of Group HH was also significantly decreased after a dose of 0.01 microgram/kg/min. Among all patients with hypertension, peak negative dD/dt correlated inversely with left ventricular mass (LVM) after the infusion of ISP (0.005 microgram/kg/min: r = -0.64, p less than 0.001, 0.01 microgram/kg/min: r = -0.68, p less than 0.001). The peak positive dD/dt of Group HH was significantly decreased only when compared with that of Group N at rest (Group N: 3.15 +/- 0.75, Group NH: 3.02 +/- 0.86, and Group HH: 1.92 +/- 0.68 cm/sec, respectively). The difference between the peak positive dD/dt of Group HH and that of Group N was more prominent after the infusion of ISP than at rest. Among all patients with hypertension, the peak positive dD/dt was inversely related to LVM at rest (r = -0.64, p less than 0.002). There was a similar relation between the two indexes after the infusion of ISP. Peak positive dD/dt was related to peak negative dD/dt after a dose of 0.01 microgram/kg/min in Group HH (r = 0.67, p less than 0.05). There was no significant difference in heart rate, change in blood pressure, or total peripheral vascular resistance after the infusion of ISP. It is concluded that diastolic left ventricular dysfunction and latent systolic left ventricular dysfunction are related to increased LVM in Group HH. It seems that after the infusion of ISP severe diastolic left ventricular dysfunction is related to latent systolic left ventricular dysfunction.

Adult↗
PubMed1985

The p40x of human T-cell leukemia virus type I is a trans-acting activator of viral gene transcription.

Human T-cell leukemia virus type I has a unique sequence pX and the product p40x was proposed to be a specific trans-acting transcriptional activator of expression of the viral gene. Recently, a second pX protein p27x-III in addition to p40x was identified; these two proteins are encoded by overlapping frames III and IV (x-lor). For determination of which product is the trans-acting activator, site-directed mutations were introduced into the pX sequence which was placed under the metallothionein promoter. On cotransfection with pLTR-CAT (a plasmid containing the LTR of HTLV-I and chloramphenicol acetyltransferase gene), only the mutations that affected p40x expression inactivated the transcriptional activation from the LTR.

Amino Acid Sequence↗