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T Takeda

Publications and source records attributed to T Takeda.

At least 523 records · Page 29Linked to original sources
PubMed1995

[The efficacy of early 123I-MIBG scintigraphy for pheochromocytoma diagnosis].

Early scintigraphy, that obtained 6 hours after intravenous administration of a low dose of 123I-metaiodobenzylguanidine (111 MBq) was evaluated for diagnostic efficacy. The accumulation of 123I-MIBG in the tumor masses of nine patients with pheochromocytoma and one patient with cortical adenoma were noted. The smallest pheochromocytoma visualized was 3 cm in diameter. The hepatic uptake of this radiolabeled compound was helpful to understand the anatomical position of the abnormal uptake. Three of five postoperative cases of pheochromocytoma showed uptake in the metastasis in the skeleton or recurrences in the neck or contralateral adrenal gland. No ectopic pheochromocytoma was found in the pelvic cavity. Faint accumulation in the normal medulla was noted in 45% of the cases. The present results demonstrate that superior images can be obtained in scintigrams takes 6 hours after administration of a lower dose of 123I-MIBG.

3-Iodobenzylguanidine↗
PubMed1995

[Correlation between acute rejection and the role of donor specific cytotoxic T lymphocyte in canine lung allotransplantation].

We investigated the correlation between the donor specific CTL activities and pathological findings of the grafted lungs by using mononuclear cells obtained from BAL fluids (BAL), peripheral blood (PBL), spleen (SpL) and grafted lungs (GIL) in canine lung allotransplantation. Twenty eight mongrel dogs underwent left lung allotransplantation. Fifteen dogs were not given immunosuppressive drugs. Thirteen dogs were given cyclosporine a 20 mg/kg/day per os for ten days postoperatively. Dogs were monitored by chest roentgenograms and cytotoxic activity tests of which targets were donor's fibroblasts. Pathological findings were classified by Prop's classification. The results were as follows. In latent and vascular phases no elevations of cytotoxic activities were demonstrated in PBL, BAL and GIL. But in early alveolar phase the cytotoxic activity was elevated to 19.83 +/- 14.54% in BAL. In late alveolar phase the highest cytotoxicities of 30.83 +/- 15.28% in PBL, 72.63 +/- 7.36% in BAL and 70.07 +/- 6.34% in GIL at E:T = 100:1 were demonstrated. In pneumonia cytotoxicity did not elevate at all in any effectors. As a result of them, it clearly shows that BAL is a good tool for the diagnosis of lung rejection.

Animals↗
PubMed1995

[Study on color misnaming among the congenital color vision anomalous--Part 1. The tendency in color misnomer].

I report the hue and the color misnomers of 16 subjects with protanopia (color misnomers: 500) and 66 subjects of deutanopia (color misnomers: 2,056), and the color misnomers used by over 10 subjects each and their numbers. Green was the most frequent misnomer, followed by grey, yellow-green, purple, and brown. The deutanopia patients frequently used the Munsell color notation RP for grey. Many of the subjects who misnamed 11 times or more failed the Panel D-15 test. They were diagnosed as having strong color anomaly in the Ohkuma isochromatic plates and in the Tokyo Medical College isochromatic plates. The misnomers were most frequent among the neighboring hues. The severer the anomaly, the further the separation from the test color, and then the misnomers crossed the achromatic confusion line. Judging from the misnomer variation, the color sense of color anomaly does not necessarily seem to be constant. Moreover, liaison was noticed among red, brown, green, or occasionally purple in terms of misnaming pattern. Grey and pink were also linked in misnaming. Lightness was considered to play a strong role in these confusions.

Adolescent↗
PubMed1995

[Study on color misnaming among the congenital color vision anomalous--Part 2. Lightness and saturation of color misnomers].

I analysed the misnamed colors reported previously by plotting them on the value and chroma diagram. The study included at least 3 subjects for protanopia and at least 5 subjects for deutanopia on the same misnomer applied to one test color. The test colors were expressed by the Munsell color notation system. Deutanopia: High lightness value group (value 5 or more); Gr and lightly saturated G-->pink (test color-->misnomer), intermediately saturated YR and Y-->yellowish green, GY-->skin color, highly saturated GY-->orange, RP-->grey in wide range, lightly or intermediately saturated BG-->grey in many cases. Low lightness value group (value 4 or less); Gr-->green, lightly saturated Br-->green, intermediately saturated YR, GY, G and RP-->red, highly saturated R-->brown, YR-->green, intermediately saturated BG and B-->purple, intermediately saturated PB and P-->blue. Protanopia: RP-->red, PB-->purple, P-->pink, low lightness value lightly saturated BG-->black (this pattern was different from deutanopia), GY-->skin color (this pattern was shifted to the lightly saturated area), YR, Y-->green (more frequent in protanopia). YR, Y-->yellowgreen (more frequent in deutanopia). On the warm color side low sensitivity was anticipated not only for R but also for YR, Y, and GY. This anticipation will be substantiated by increasing the test subjects.

Color↗
PubMed1995

[Study on color misnaming among the congenital color vision anomalous--Part 3. Color misnomers and collation].

The test colors misnamed were grouped into two in their collation, i.e., R-G and BG-RP. Isochromatic collation was found in 75% of the subjects with normal color vision, 38.3% of those with protanopia, and 33% of those with deutanopia. In the hue collation isochromatic collation was seen most frequently, followed by neighborhood color collation. The more the misnomers, the further separated the hue collation, then the misnaming territory enlarged crossing over the achromatic confusion line, and the collation territory enlarged. The lightness collation was about equal between subjects with abnormal color vision and normal subjects. The former were inferior to the latter by 2% (deutan) and 4% (protan) in isosaturation collation. When the misnomer crossed the isochromatic line or collated near the white point, the date were analysed according to the color cunfusion theory. The subjects with abnormal color vision showed hue collation inferior to that of normal subjects, but they responded with twice the accuracy in the isochromatic collation than the misnomer collation, and the collation territory was narrow and simple.

Adult↗
PubMed1995

Resting asynchronous left ventricular contraction abnormality analyzed by a phase method in spastic angina pectoris.

UNLABELLED: Quantitative phase analysis of equilibrium ventriculography was performed to study the character of left ventricular (LV) wall motion abnormalities in patients with spastic angina pectoris, who may have clinically and electrocardiographically silent ischemia combined with myocardial stunning, during rest and hyperventilation stress testing. METHODS: Phase analysis of the left ventricle at rest was performed by equilibrium radionuclide ventriculography in 13 control subjects and 36 patients with spastic angina pectoris. First-pass methodology along with hyperventilation stress testing was performed to assess spasm occurrences. Phase analysis of equilibrium multigated blood-pool scintigrams was performed to evaluate LV asynchrony at rest. RESULTS: The mean s.d. of LV phase distribution in the patients with variant and vasospastic angina was greater than that in the healthy control subjects (11.28 +/- 1.79 and 10.02 +/- 1.57 degrees versus 6.16 +/- 1.07 degrees). In addition, the mean s.d. of LV phase distribution in the variant angina group was greater than that in the vasospastic angina group. Furthermore, a linear correlation was found between the s.d. of LV phase distribution at rest and the percent decrease in ejection fraction during hyperventilation stress. CONCLUSION: Asynchronous LV contraction without significant hypokinesis was detected at rest in spastic angina pectoris. The severity of this asynchronous contraction corresponded well with decreases in ejection fraction during hyperventilation stress testing. Thus, analysis of the s.d. of LV phase distribution at rest is expected to provide useful information regarding LV asynchrony in spastic angina pectoris.

Adult↗
PubMed1995

Apolipoprotein A-II gene and development of amyloidosis and senescence in a congenic strain of mice carrying amyloidogenic ApoA-II.

BACKGROUND: Apolipoprotein A-II (ApoA-II), a major apoprotein of serum high density lipoprotein (HDL) deposits as an amyloid fibril (AApoAII) in murine senile amyloidosis. Type C ApoA-II gene (Apoa2c) in the SAMP1 strain of mice, a murine model of severe senile amyloidosis and accelerated senescence was transferred on the genetic background of the SAMR1 strain, in which senile amyloidosis is rare and has a normal aging process, and a congenic strain of mouse (R1.P1-Apoa2c) was developed (Higuchi K, Kitado H, Kitagawa K, Kogishi K, Naiki H, Takeda T. FEBS Lett 1993;317:207-10). EXPERIMENTAL DESIGN: We identified AApoAII amyloid deposits in the 14-month-old congenic R1.P1-Apoa2c strain and compared these findings with the deposits in the progenitor SAMP1 and SAMR1 strains. The progression of senescence was estimated using a grading system and the age-associated changes in the metabolism of ApoA-II and HDL were investigated in the three strains of mice. RESULTS: At 14 months of age, severe amyloid deposition as compared with the donor SAMP1 strain was present in the congenic R1.P1-Apoa2c strain, but AApoAII was not evident in the progenitor SAMR1 strain which has type B ApoA-II. No obvious differences in the progression of senescence were observed between the R1.P1-Apoa2c and SAMR1 strains. In the R1.P1-Apoa2c strain, the serum HDL-cholesterol concentrations decreased in parallel with ApoA-II levels with advancing age and the decrease was much accelerated compared with the decrease seen in SAMR1 mice. CONCLUSIONS: Based on these results, we propose that the genetic type of ApoA-II plays an important role in the development of senile amyloidosis and age-associated changes in HDL metabolism. However, it has a minor role in the accelerated senescence in the mouse strains we used.

Aging↗
PubMed1995

Clinical usefulness of evaluation of portal circulation by per rectal portal scintigraphy with technetium-99m pertechnetate.

OBJECTIVE: Portal circulation, in particular the contribution of the inferior mesenteric vein, can be evaluated in a relatively noninvasive way by per rectal portal scintigraphy (J Nucl Med 1988; 29:460-5). The clinical usefulness of the method was evaluated. METHODS: A solution containing technitium-99m pertechnetate was instilled into the rectum, and serial scintigrams were taken while radioactivity curves for the liver and heart were recorded sequentially. By analyses of the curves, the per rectal portal shunt index (SI) was calculated. RESULTS: The SI was higher for disorders that were more severe, increasing in the order of chronic persistent hepatitis, chronic aggressive hepatitis, and cirrhosis, and the SI was higher in cirrhotic patients than in patients with chronic hepatitis or in healthy subjects. The SI was significantly higher when a complication (varices, ascites, or encephalopathy) was present. Correlation between the SI and classic indicators for functional reserve was significant. The SI was significantly related to survival according to results of regression analysis by Cox's proportional hazards model. On the basis of the SI when patients were first examined, the patients with cirrhosis were divided into three groups of roughly equal size: group A, SI under 30%; group B, SI between 30 and 70%; and group C, SI over 70%. The survival rate was lower in group B than in A, lower in group C than in A, and lower in group C than in B. CONCLUSIONS: This method is clinically useful, especially in establishing the prognosis.

Humans↗
PubMed1995

[A fundamental study on accumulation of [125I]IBZM in the rat striatum and on effect of non-labeled ligand].

Iodo-125-labeled iodobenzamide ([125I]IBZM) is used as a specific binding radioligand to dopamine D2 receptors with high affinity and selectivity. The radioligand was homogenously distributed in the whole brain initially after administration, and rapidly washed out from the dopamine receptor-poor area followed by persistent retention in the striatum. Regression curve generated from striatum/cortex PSL ratio indicated the constant washout rate from striatum and cortex respectively. In the pretreated rat by cold benzamide (2 mg/kg), the accumulation of the radioligand was significantly suppressed in the striatum (48.8%). Iodine-125-labeled iodo-benzamide has the promise for investigation of dopamine D2 receptors in the living brain.

Animals↗
PubMed1995

[Influence of warm ischemia and reperfusion on no-derived cyclic GMP release in the perfused rabbit lung].

To elucidate the vascular endothelial function at pulmonary ischemia and reperfusion, cyclic GMP (cGMP) release in lung perfusate at reperfusion after ischemia was examined as a marker of nitric oxide (NO) release from the endothelium in an in situ perfused rabbit lung. The lung was perfused from the pulmonary artery with Krebs-Henselite buffer and cGMP in the lung effluent from the left ventricle was assayed in a time-dependent manner. The inhibition of cGMP release by NG-nitro-L-arginine methyl ester and the reversal of the effect by L-arginine indicated that cGMP released into the perfusate was elicited by NO from the endothelium. There was a significant decrease in cGMP release during reperfusion after warm ischemia (30 min and 60 min) compared with the immediately perfusion without intervening ischemia. These data suggest that NO release is impaired by the endothelial dysfunction at reperfusion after warm ischemia in the perfused rabbit lung and the biochemical studies of the perfusate is useful in assessing the vascular endothelial function.

Animals↗
PubMed1995

Imidapril hydrochloride in essential hypertension: a double-blind comparative study using enalapril maleate as a control.

OBJECTIVE: To assess the value of using imidapril hydrochloride (ACE/TA-6366), a long-acting angiotensin converting enzyme (ACE) inhibitor developed in Japan, to treat patients with essential hypertension. PATIENTS AND METHODS: A double-blind, comparative, phase III study was carried out using enalapril maleate as a control, with a 4-week observation period and a 12-week treatment period. Both drugs were started at a dose of 5 mg once a day, increasing to 10 mg in patients whose antihypertensive response was insufficient after 4 weeks. The study included 231 outpatients aged 30-74 years; of these, 108 in the imidapril group and 115 in the enalapril group were assessed. There were no differences in background factors between groups. RESULTS: An adequate antihypertensive effect was observed in 71.3% (77/108) in the imidapril group in in 66.1% (76/115) in the enalapril group, with no significant difference between groups. The pulse rate was unchanged in both groups. The drug had no adverse effects in 86.1% (93/108) of the imidapril group and 79.1% (91/115) of the enalapril group, with no significant difference between groups. Adverse drug effects were observed din 5.6% (6/108) of the imidapril group and 12.2% (14/115) of the enalapril group. Cough was the most frequent side effect, reported in 0.9% (1/108) of the imidapril group and 7.0% (8/115) of the enalapril group. Other side effects were reported in 4.6% (5/108) of the imidapril group and 5.2% (6/115) of the enalapril group. Abnormal laboratory values were observed in 3.7% (4/108) of the imidapril group and 0.9% (1/115) of the enalapril group. CONCLUSIONS: Imidapril showed excellent clinical efficacy and safety compared to enalapril. The low incidence of cough is of particular interest.

Adult↗
PubMed1995

Promotion of nitric oxide formation by heparin in cultured aortic endothelial cells from spontaneously hypertensive rats.

1. The present study examined the effect of heparin on nitric oxide (NO) formation and cyclic guanosine 3', 5'-monophosphate (cGMP) levels in cultured aortic endothelial cells (EC) from spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) rats. 2. Bradykinin (BK), adenosine diphosphate (ADP), Ca2+ ionophore A23187 (Io) and endothelin-3 (ET-3) stimulated the production of NO and cGMP. No significant difference was observed in both NO and cGMP production in EC between WKY and SHR. 3. Heparin enhanced BK-, ADP-, Io- and ET-3-stimulated NO and cGMP production. These enhancements by heparin in EC were significantly greater in SHR than in WKY. 4. Both NO formation and cGMP production stimulated by the agonists and/or heparin were blocked in the presence of NG-monomethyl-L-arginine (LNMMA, 10(-5) mol/L). 5. Increased sulphur level was observed on heparin-treated SHR EC surface compared with that on control SHR EC or on heparin-treated WKY EC surface. 6. These results suggest that heparin promotes agonist-induced NO-cGMP response in cultured EC from SHR.

Animals↗
PubMed1995

Promotion of nitric oxide formation by heparin in cultured aortic endothelial cells from spontaneously hypertensive rats.

1. The present study examined the effect of heparin on nitric oxide (NO) formation and cyclic guanosine 3', 5'-monophosphate (cGMP) levels in cultured aortic endothelial cells (EC) from spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) rats. 2. Bradykinin (BK), adenosine diphosphate (ADP), Ca2+ ionophore A23187 (Io) and endothelin-3 (ET-3) stimulated the production of NO and cGMP. No significant difference was observed in both NO and cGMP production in EC between WKY and SHR. 3. Heparin enhanced BK-, ADP-, Io- and ET-3-stimulated NO and cGMP production. These enhancements by heparin in EC were significantly greater in SHR than in WKY. 4. Both NO formation and cGMP production stimulated by the agonists and/or heparin were blocked in the presence of NG-monomethyl-L-arginine (LNMMA, 10(-5) mol/L). 5. Increased sulphur level was observed on heparin-treated SHR EC surface compared with that on control SHR EC or on heparin-treated WKY EC surface. 6. These results suggest that heparin promotes agonist-induced NO-cGMP response in cultured EC from SHR.

Animals↗
PubMed1994

Purification and characterization of a novel superantigen produced by a clinical isolate of Yersinia pseudotuberculosis.

A superantigen designated as Yersinia pseudotuberculosis-derived mitogen (YPM) was purified in an equal manner from both the culture supernatant and cell lysate of a clinical isolate (KUR-1) of Y. pseudotuberculosis serotype 4b. A significant proliferative response of human peripheral blood mononuclear cells to purified YPM was detectable even at a concentration of 1 pg/ml. The N-terminal sequence of YPM which included 23 amino acid residues was determined, by automated Edman degradation, as Thr-Asp-Tyr-Asp-Asn-Thr-Leu-Asn-Ser-Ile-Pro-Ser-Leu-Arg-Ile-Pro-Asn-Il e-Ala-Thr-Tyr-Thr-Gly-. This sequence differed from not only all the, hitherto, reported superantigens but also known proteins. While molecular weights of known bacterial superantigens are more than 22,000, electrospray ionization mass spectrometry showed that the molecular weight of YPM was 14524.4. These results indicate that YPM comprises a novel superantigen with substantial structural differences from other bacterial superantigens produced by Gram-positive cocci.

Amino Acid Sequence↗
PubMed1994

Adrenomedullin, a newly discovered hypotensive peptide, is a potent bronchodilator.

The present study was designed to examine the effect of adrenomedullin (AM), a novel vasorelaxant peptide originally isolated from human pheochromocytoma, on histamine- or acetylcholine-induced bronchoconstriction in anesthetized guinea pigs in vivo. AM significantly inhibited acetylcholine-induced bronchoconstriction in a dose-dependent fashion. AM also significantly inhibited histamine-induced bronchoconstriction, but 10(-10) M AM had no significant inhibitory effect on this response. We also found that AM induced a long-lasting bronchodilator response, while isoproterenol induced transient bronchodilation. These preliminary findings suggest that AM may play important roles in airway function.

Acetylcholine↗