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Biomedical subjects

T Sun

Publications and source records attributed to T Sun.

At least 235 records · Page 13Linked to original sources
PubMed1980

The diagnosis of giardiasis.

Giardiasis is the most prevalent intestinal parasitic disease in the United States. Most cases can be diagnosed by a single stool examination. However, in periodic cyst excretors, cysts may not be detected unless repeated stool examinations are performed. In clinically highly suspected cases, duodenal fluid should be studies after three negative stool examinations. Scanning electron microscopy is probably superior to light microscopy in screening duodenal fluid if the parasite is scanty or degenerated. A small intestinal biopsy is a last resort for the diagnosis; a mucosal impression smear should be routinely performed on such specimens. The latter is the most sensitive and reliable technique in making the diagnosis.

Biopsy↗
PubMed1980

Clonorchiasis: a report of four cases and discussion of unusual manifestations.

Clonorchiasis is still a problem for Asian immigrants to North America, and is occasionally seen among American residents who contract the disease during long-term or short-term visits to endemic areas. The incidence of clonorchiasis is especially high among Chinese immigrants from Hong Kong. The acute symptoms most frequently encountered in this group of patients are those of recurrent pyogenic cholangitis and acute pancreatitis. Two American cases are reported together with a brief discussion of the pathology and immunology of this disease. Two unusual lesions in Hong Kong patients, an egg-granuloma of the liver and an adenoma of the bile duct, are also discussed.

Adult↗
PubMed1980

Total serum cholesterol by isotope dilution/mass spectrometry: a candidate definitive method.

We describe a highly accurate and precise method for determination of total cholesterol in serum by isotope dilution/mass spectrometry. The method was developed for a Study Group of the Committee on Standards of the American Association for Clinical Chemistry, for use in establishing the accuracy of a candidate reference method for total cholesterol, and fulfills their criteria for a definitive method. Cholesterol-d7 is added to serum, with the weight ratio of cholesterol-d7 to total serum cholesterol kept near to 1:1. The esters are hydrolyzed and the cholesterol is separated and converted into the trimethylsilyl ether derivative for measurement by combined gas chromatography/mass spectrometry. The intensity ratio of the molecular ions at m/z 465 and 458 is measured for each sample and for two calibration mixtures, according to a prescribed bracketing protocol. A weight ratio for the sample is obtained by linear interpolation of the ion-intensity ratios, and the total cholesterol is then calculated. The method was applied four times over several weeks to each of five serum pools. Statistical analysis involving consideration of both replication error and variability between weeks gave a coefficient of variation for a single measurement of 0.36%. The absence of interferences in the method was demonstrated by measurements at several other masses.

Carbon Radioisotopes↗
PubMed1979

Study of gammopathies with immunofixation electrophoresis.

Immunofixation electrophoresis and immunoelectrophoresis were compared in 60 samples (51 sera and 9 urines) containing apparently homogenous bands, detected by electrophoresis. To determine the correlation with clinical findings, the patients were divided into three groups: (1) symptomatic with monoclonal immunoelectrophoretic patterns; (2) asymptomatic with monoclonal immunoelectrophoretic patterns; (3) asymptomatic with polyclonal immunoelectrophoretic patterns. The results from immunoelectrophoresis and immunofixation electrophoresis were consistent with each other in all cases of Groups I and III in terms of clonality (i.e., whether monoclonal or polyclonal) and immunoglobulin class; whereas in Group II, which was composed of asymptomatic patients, two sera and three urines were identified to have monoclonal changes by immunoelectrophoresis but polyclonal changes by immunofixation electrophoresis. It is recommended that immunoelectrophoresis still be used for routine clinical service, but supplemented by immunofixation electrophoresis in equivocal cases, namely: (1) light-chain changes masked by an umbrella effect of immunoglobulin G (IgG); (2) abnormal bands located in atypical areas or overlapped with a normal serum protein; (3) a normal-looking "free" light chain present in the urine, mimicking Bence Jones protein; (4) controversial cases of biclonal gammopathy; (5) mini-monoclonal or oligoclonal protein bands; (6) immune complexes.

Bence Jones Protein↗
PubMed1977

Evaluation of a high-resolution electrophoresis system.

A high-resolution electrophoresis system (Panagel) is evaluated by comparing the results with Microzone electrophoresis and with quantitation of individual protein fractions by the radial immunodiffusion technic and by the SMA system. The high-resolution electrophoresis showed a higher percentage of gamma globulin and a lower percentage of albumin than the Microzone system, with the alpha-1, alpha-2, and beta globulins being similar in quantity. The eight protein fractions studied: albumin, alpha-1 antitrypsin, alpha-2 macroglobulin, haptoglobin, beta-lipoprotein, C3 complement, and gamma globulin, correlated very well with the corresponding components in the various methods used. The normal values of the protein fractions were determined.

Adult↗