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Biomedical subjects

T Sueda

Publications and source records attributed to T Sueda.

At least 109 records · Page 6Linked to original sources
PubMed1997

Culture and characterization of human junctional epithelial cells.

This study was undertaken to establish a culture of junctional epithelial cells derived from gingival tissue attached to the tooth surface and to characterize these cells immunocytochemically and ultrastructurally. Primary cultures of cells were obtained from the junctional tissue explanted on type I collagen-coated dishes and immersed in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum (FBS). Cells were subcultured with conditioned serum-free keratinocyte medium (keratinocyte-SFM + 5% FBS) on dishes coated with solubilized extract of the basement membrane. After 24 hours, the medium was changed to keratinocyte-SFM (0.09 mM Ca2+). The cell-doubling time was 40.5 hours. As a control, cells from gingival tissue were cultured by the same method. Cells from junctional tissue and gingival tissue were compared immunocytochemically using monoclonal antibodies to keratin, vimentin, and desmoplakins I and II and using Dolichos biflorus agglutinin (DBA). The keratin AE1 and AE3 was expressed by all of culture cells. The vimentin (specific for the intermediate filament of mesenchymal cells) was also expressed by all cells. The expression pattern of keratin 19 was observed not only by cells from junctional tissue but also by cells from gingival tissue. All keratin peptides were expressed in both cells. However, DBA reacted only with cells from the junctional tissue. Anti-desmoplakin I and II reacted with both cells, however, the staining patterns differed. DBA-positive cultured epithelial cells from the junctional tissue showed poor tonofilament bundles and were rich in cytoplasmic organelles. These findings suggest that junctional epithelial cells can be isolated from junctional tissue and cultured under improved conditions.

Adolescent↗
PubMed1997

Postprandial atrioventricular block in a patient with diabetes mellitus.

Postprandial atrioventricular (AV) block and hypotension occurred in an 80-year-old man with a 13-year history of diabetes mellitus. Two weeks before admission, he experienced syncope on two occasions after lunch. Master's two-step test induced second degree AV block (2:1 block). Four days after admission, syncope recurred while walking 1 hour after eating; electrocardiogram (ECG) showed complete AV block following 2:1 block. PP interval analysis suggested phase 3 block as the mechanism of AV block. Electrophysiologic study revealed 2:1 HV block. Postprandial syncope ceased after permanent pacemaker implantation. Postprandial AV block and hypotension merit close attention in diabetics.

Aged↗
PubMed1997

[Left atrial isolation for chronic atrial fibrillation associated with valvular disease and coronary artery disease in an 82-year-old man].

An 82-year-old man suffering from chronic atrial fibrillation associated with valvular and coronary artery diseases was admitted to our hospital. Preoperative examination revealed that mitral and aortic valve replacement with tricuspid annuloplasty and coronary artery bypass were required, in addition to surgery for chronic atrial fibrillation. In order to shorten the aortic cross-clamp time and operative time, we performed left atrial isolation, which is a simpler procedure than maze procedure and the left sided maze procedure, because it requires incision of the posterior left atrium only. Since aortic stenosis was severe and the aortic annulus was small, we used a St. Jude Medical Hemodynamic Plus valve as the aortic valve prosthesis, which spares the surgical stress of the aortic annulus. Careful investigation of the patient disorder and selection of optimal surgical procedure makes possible an extended operation of complicated valvular disease even in a high elderly patient over 80 years old.

Age Factors↗
PubMed1997

Protective effect of beraprost sodium, a stable prostacyclin analogue, on cardiac allograft vasculopathy in rats.

Intimal thickening and luminal narrowing of the coronary arteries are insidious complications of cardiac allograft. However, the pathogenesis of cardiac allograft vasculopathy (CAV) remains to be clarified. In this study, the protective effect of a prostacyclin analogue (beraprost sodium; BPS) on CAV was evaluated after heterotopic cardiac transplantation in rat. All recipients were treated with cyclosporine A (10 mg/kg/day intramuscularly). Eight rats received oral therapy with BPS of 50 micrograms/kg/day (BPS group) and another 8 rats received vehicle only (control group). All surviving cardiac grafts were removed on the 60th postoperative day and were examined to determine the severities of cellular rejection and CAV (> 50 microns in diameter). Additionally, 6-keto-prostagrandin F1 alpha and thromboxane B2 were compared between the two groups. There was no significant difference in the grading score for cellular rejection based on the ISHLT grading system (2.31 +/- 0.75 vs 2.47 +/- 0.65, p = 0.81). Although the endothelial cells were preserved in both groups, a deposition of fibrin-like dense materials was recognized in the subendothelial layers of the control group, but not in the BPS group. Intimal thickening was inhibited significantly in the BPS group. The intimal ratio (intimal area/sectional area of artery) was significantly lower in the BPS group than in the control group (0.134 +/- 0.03 vs 0.205 +/- 0.047; p < 0.01), without any difference in the medial ratio (medial area/sectional area of artery). alpha-actin positive smooth muscle cells (SMC) in intima were fewer in number in the BPS group than in the control group. The plasma thromboxane B2 level was significantly lower in the BPS group than in the control group (270 +/- 116 pg/ml vs 585 +/- 258 pg/mg; p < 0.01). It was concluded that BPS suppressed CAV development after heterotopic allogenic cardiac transplantation in rats.

Animals↗
PubMed1997

[Monitoring for spinal cord ischemia by the use of the motor evoked potentials, the evoked spinal cord potential and the segmental evoked spinal cord potential during thoracoabdominal aortic surgery--a case report].

A 55-year-old woman, who was detected an enlargement of the aorta with body CT, was admitted to our hospital. Preoperative examination revealed that the aneurysm was expanded from ascending aorta to the abdominal aorta above the celiac artery. She underwent total arch replacement using elephant trunk technique. Forty-four days after operation, she underwent thoracoabdominal aortic replacement with monitoring for spinal cord ischemia by the use of the motor evoked potentials (MEP), the evoked spinal cord potential (ESCP) and the segmental evoked spinal cord potential (S-ESCP). Under the partial cardiopulmonary bypass with femoral venous drainage and femoral arterial cannulation on normothermia, the aneurysmal part of the aorta was segmentally cross-clamped and the three lumbar arteries and the celiac artery were reconstructed one by one. During the operation, the monitor showed no remarkable change, even while the aorta was cross clamped. Her postoperative course was uneventful without any spinal cord damage. Combined use of MEP, ESCP, and S-ESCP was useful for monitoring spinal cord injury during thoracoabdominal aneurysmal repair.

Aorta, Thoracic↗
PubMed1997

[Repair of aortic arch aneurysm protruding to the retrobronchial space--a case report].

A case of pseudoaneurysm of the aortic arch which protruded to the retrobronchial space is reported. A 73-year-old female complaining of severe chest pain was transferred to our hospital, CT showed an abnormal mass which occupied the retrobronchial space and displaced the esophagus toward righ, associated with left pleural effusion. A pseudoaneurysm of the aortic arch was suspected. Angiography revealed an aortic arch aneurysm protruding to the retrobronchial space. Emergent total arch replacement was performed. We diagnosed it as an impending rupture of the aortic arch aneurysm with a specific shape.

Aged↗
PubMed1997

Beneficial effects of perfluorotributylamine/pluronic F-68 stem-emulsion (FC43se) on discordant lung xenografts.

The aim of this study was to assess the effectiveness of Perfluorotributylamine/Pluronic F-68 Stem-Emulsion (FC43se) against hyperacute rejection in rabbit-pig xenodiscordant transplantation in an ex-vivo lung model. Rabbits were divided into two groups. In Group 1 (control), after extraction, the lungs were connected directly to the experimental circuit as soon as possible. The lungs were then perfused with 100 ml of pig blood only. In Group 2 (FC(+) Group), after extraction, the lungs were connected to the same circuit as soon as possible. The lungs were then perfused with 10 ml of FC43se plus 90 ml of pig blood. The duration of perfusion was defined from when the perfusion started until the PAP reached 50 mmHg. Significant differences in survival were seen between Group 1 and Group 2: in Group 1, the survival time was 51.9 +/- 16.8 min, whereas in Group 2 the survival time was 76.9 +/- 12.4 min (p < 0.01). The wet-to-dry weight ratio after 30 min of perfusion in Group 2 was significantly lower than that of Group 1 and the mean pulmonary artery pressure of Group 2 at 35, 40 and 45 min after perfusion was significantly lower than that of Group 1. Histological examination revealed that FC43se suppressed the adhesion of leukocytes to the surfaces of endothelial cells, and also attenuated the intimal edematous changes accompanying leukocyte infiltration. Therefore, FC43se has a beneficial effect on suppressing hyperacute rejection in xenogeneic discordant lung transplantation.

Animals↗
PubMed1997

Abdominal aorta and visceral arteries visualized by transgastric echocardiography: technical considerations.

Despite the necessity of information regarding the abdominal aorta and visceral arteries during cardiovascular surgery, there has been no intraoperative modality available. We examined the feasibility and limitations of transesophageal echocardiography (TEE) for this purpose. In 21 consecutive patients, the celiac artery (CEA), superior mesenteric artery (SMA), and left and right renal arteries (LRA, RRA) were examined with TEE, and could be visualized in 21 cases (100%), 20 cases (95.2%), 14 cases (66.7%) and 14 cases (66.7%), respectively. Several attempts were needed for successfully visualizing the LRA and the RRA in 2 and 1 case(s), respectively. Three specific manipulations of the probe were helpful for visualizing these vessels: 1) an appropriate counterclockwise rotation and an upward flexion of the probe when the transducer entered the stomach; 2) a stiffening of the flexible portion of the probe at the position of upward flexion by fixing the handle of the TEE probe when the transducer was advanced; and 3) a lateral flexion of the probe to provide a rotation of the image in either the clockwise or counterclockwise direction and to optimize the assessment of the blood flow velocity in the branch artery. Inferior visualization of the renal arteries was a limitation of this method. Two solutions for this problem were 1) repeated attempts at visualization and 2) an examination of the blood flow in the renal parenchyma with color Doppler imaging. Because of possible damage to the gastric wall, it is recommended that this maneuver be conducted by an experienced sonographer.

Aged↗
PubMed1997

Ammonia determination as an early indicator in experimental superior mesenteric artery occlusion.

Superior mesenteric artery occlusion (SMAO) is often fatal. An indicator which enables the early diagnosis of SMAO is needed. As we think putrefaction products must appear and increase in the blood and ascites in SMAO, changes in the concentrations of ammonia, one of the putrefaction products, were measured in this study. Thirteen adult mongrel dogs were used for the in vitro experiment. The jejunum, ileum, and ascending colon were resected and incubated in saline. Changes in ammonia concentrations in the saline were examined at various incubation times. In the in vivo experiment, 11 mongrel dogs comprised the SMAO group and another 10 mongrel dogs comprised the control group. Changes in ammonia concentrations in the blood and ascites were examined in both groups. In the in vitro experiment, ammonia concentrations in the saline bath increased in all samples. It was highest in the sample from around the ascending colon, and lowest from around the jejunum. However, at the end of experiment, this difference became insignificant. In the in vivo experiment, ammonia concentrations in samples of the blood increased early and significantly in the SMAO group, compared with the control group. Ammonia concentrations in samples of the ascites also increased significantly. The in vitro experiment showed that ammonia leaked from the ischemic intestines, and secondarily, a large amount of ammonia was produced from intestinal putrefaction. The in vivo experiment revealed that the ammonia level in the blood could be used as a good early indicator of acute mesenteric ischemia.

Ammonia↗
PubMed1996

A modified maze procedure performed only on the left atrium for chronic atrial fibrillation associated with mitral valve disease: report of a case.

We describe herein the successful treatment of a patient with chronic atrial fibrillation (AF) associated with mitral valve stenosis and regurgitation, achieved by performing a modified maze procedure on the left atrium alone. The patient was a 51-year-old man who had suffered from intractable AF for 17 years, causing multiple cerebral emboli and palpitations. He had undergone open mitral commissurotomy and balloon commissurotomy 15 and 7 years ago, respectively. On admission, an echocardiogram revealed mitral valve restenosis and thrombosis in the left atrial appendage. Prosthetic valvular replacement was performed following isolation of all pulmonary veins with cryoablation to the posterior wall of the left atrium and excision of the left atrial appendage. Postoperatively, the AF disappeared and echocardiogram demonstrated a left atrial kick in the mitral valvular inflow without any evidence of thrombosis in the left atrium. Thus, we believe that our modified "left side only" maze procedure is a simple and efficient method for the treatment of chronic AF with mitral valve disease.

Atrial Fibrillation↗
PubMed1996

Pooled air in open heart operations examined by transesophageal echocardiography.

BACKGROUND: The clinical significance of pooled air detected by transesophageal echocardiography during open heart operations is not clear. METHODS: Thirty-eight consecutive patients undergoing an open heart operation or an operation on the ascending aorta were divided into two groups on the basis of the absence (group 1, n = 14) or presence (group 2, n = 24) of pooled air. They were examined for intramyocardial echo contrast, ST segment elevation, conduction disturbances, and regional wall motion abnormalities. RESULTS: Echo contrast was found in no patient in group 1 and 66.7% of group 2 patients (p < 0.001). New regional wall motion abnormalities were detected in no patient in group 1 versus 33.3% of group 2 patients (p < 0.05), and ST segment elevation was seen in 33.3% of group 2 patients versus no group 1 patients (p < 0.05). Intramyocardial cardial echo contrast was newly detected after the appearance of pooled air more frequently in patients with ST segment elevation (p < 0.001). Atrioventricular block and sinus arrest appeared in 3 patients and 2 patients, respectively. Postoperative regional wall motion abnormalities were found in 25.0% of patients and were not closely related to intraoperative echo contrast findings. CONCLUSIONS: Pooled air, which is often detected in open heart operation by means of transesophageal echocardiography, is related to several cardiac events, including ST segment elevation, conduction disturbances, and regional wall motion abnormalities, although most of these are transient.

Adolescent↗
PubMed1996

Simple left atrial procedure for chronic atrial fibrillation associated with mitral valve disease.

BACKGROUND: A computerized 48-channel mapping system was used to investigate the characteristics of an atrial epicardial electrogram during chronic atrial fibrillation (AF) in patients with solitary mitral valve disease. We have devised a simple left atrial procedure to eliminate the chronic AF during a mitral valve operation. METHODS: Using this mapping system, we performed intraoperative atrial mapping in 11 patients with chronic AF associated with mitral valve disease. The AF duration ranged from 0.4 to 15 years (mean, 8.0 +/- 4.5 years). A simple surgical ablation of the AF on the left atrium only was performed during the mitral valve operations. RESULTS: The mean AF cycle length of the atria ranged from 129 to 169 milliseconds in the right atrium and from 114 to 139 milliseconds in the left atrium. The mean AF cycle length of the left atrium was shorter than that of the right atrium. Regular and repetitive activation was found in the left atria of 7 of 11 patients. The AF disappeared in all patients immediately after the operation, and 10 of these patients continued to have a sinus rhythm postoperatively (AF-free rate, 91%). CONCLUSIONS: Computerized intraoperative mapping revealed a shorter mean AF cycle length in the left atrium. A simple left atrial procedure was effective in eliminating chronic AF associated with solitary mitral valve disease.

Adult↗
PubMed1996

Lipopolysaccharide increases cell surface-associated fibronectin in fibroblasts in vitro.

This study assessed the fibronectin expression by fibroblasts derived from noninflamed and inflamed gingiva by measuring the amount of cell surface-associated fibronectin and fibronectin released into the medium. The effects of added lipopolysaccharide from Porphyromonas gingivalis, Prevotella nigrescens and Escherichia coli on both types of fibroblasts were also studied. In the absence of lipopolysaccharide, the amounts of the two types of fibronectin were significantly larger in the fibroblasts from inflamed than from noninflamed gingiva. The specific lipopolysaccharide had no effect on the amount of fibronectin released into the medium by either fibroblast type. The amount of cell surface-associated fibronectin increased significantly when lipopolysaccharide (0.1 and 1 microgram/ml) was added to the cells from the noninflamed gingiva (the effect was evident in the order: P. gingivalis>P.nigrescens>E. coli). Lipopolysaccharide from P. gingivalis significantly increased the cell surface-associated fibronectin even at a low concentration of lipopolysaccharide (0.01 microgram/ml). In fibroblasts from the inflamed gingiva, only the lipopolysaccharide from P. gingivalis was effective in increasing the amount of cell surface-associated fibronectin. Our findings showed that the fibronectin expression was increased in the fibroblasts from inflamed gingiva and that lipopolysaccharide from P. gingivalis increased the cell surface-associated fibronectin.

Analysis of Variance↗
PubMed1996

Past, present, and future of total artificial heart development at research institute of replacement medicine, Hiroshima University School of Medicine.

The history and recent progress in total artificial heart (TAH) development were reviewed and divided into three stages. The first stage was between 1966 and 1972, when a trial developing an artificial heart (AH) driver was begun using poppet valves. Fluid amplifiers and air operated valves were then employed as a controller. The second stage was between 1973 and 1983. Several models of pneumatic AH were manufactured and implanted into animals, and it was confirmed that animals could survive with their circulation supported by these mechanical hearts. The third stage was from 1984 to the present, and now a trial to construct a totally implantable type of AH is under way. On the basis of the experience of developing TAHs, the following points are discussed as future problems to be solved: first, the production of small yet powerful actuators; second, the establishment of safe and reliable controls and an energy supply method; third, the development of a durable blood chamber containing valves; and fourth, the acquisition of large research funds for TAH from governments and other granting agencies.

Animals↗
PubMed1996

Effects of perfluorochemical-based artificial blood compounds in discordant xenotransplantation.

Fluosol-DA and FC43 emulsion were developed as red blood cell substitutes. However, these perfluorochemical (PFC)-based compounds are not very promising because of their immunosuppressive effects. Therefore we used these compounds as immunosuppressive agents in guinea pig-to-rat discordant heart xenotransplantations. In rats treated with these compounds (5 microliters/g body weight), rhythmic beating of the hearts was maintained for 265.7 +/- 50.3 min (Fluosol-DA-treated group) and 424.3 +/- 40.1 min (FC43 emulsion-treated group), respectively whereas the untreated heartbeat lasted for only 15.5 +/- 6.6 min. In the xenografted heart treated with PFC compounds, no pathologic changes, such as multiple coronary thromboses due to hyperacute rejection, had occurred by 3-4 h after reperfusion, and the endothelial cells were well preserved. We conclude that these PFC compounds can prolong the survival time of guinea pig heart xenografts by inhibiting thrombus formation in the xenografted heart.

Analysis of Variance↗
PubMed1996

Coronary artery fistula to bronchial artery on contralateral side of coronary atherosclerosis and myocardial insufficiency. A case report.

Among coronary vessels anomalies is the rare coronary artery fistula. Myocardial insufficiency resulting from fistula steal phenomenon can not be clinically distinguished from that of coronary atherosclerosis, if these conditions coexists in the same patient. The authors present a case of extracardiac left coronary artery fistula connecting the circumflex branch and left bronchial artery, with inferior wall myocardial insufficiency attributable to the right coronary artery atherosclerosis.

Aged↗
PubMed1996

Partially-hydrophobized polymer particles derived from N,N-dimethylaminopropylacrylamide for endotoxin removal from acidic protein solution.

Novel copolymeric adsorbents for the selective removal of endotoxin from an acidic protein solution were prepared. The adsorbents comprise spherical copolymers derived from N,N-dimethylaminopropylacrylamide (DMAPAA) and divinylbenzene (DVB). When the molar ratio of DMAPAA to DVB was 80/20 (amino-group content: 5.1 meq/g) and the pore size (molecular mass exclusion of polysaccharide, Mlim) was 4000 to 10000, DMAPAA/DVB showed high endotoxin-adsorbing activity at pH 5.0 to 9.0 and ionic strengths of mu = 0.05 to 0.4. The capacity of the adsorbent (Mlim: 4000) was 390 micrograms of endotoxin (lipopolysaccharide purified from E. coli O111:B4) per ml of the adsorbent using the batchwise method. The apparent dissociation constant between endotoxin and the adsorbent was 2.2 x 10(-12) M. On the other hand, the adsorption of bovine serum albumin, an acidic protein, by the adsorbent increased with an increase in Mlim from 4000 to 10000, but decreased with an increase in ionic strength (mu) from 0.05 to 0.2. As a result, DMAPAA/DVB (80/20) (Mlim: 4000) selectively removed endotoxin from various acidic protein solutions at pH 7.0 and mu = 0.05. The residual concentration of endotoxin in the protein solution always decreased to a concentration lower than 0.1 ng/ml, and recovery of the protein was more than 97%.

Acrylamides↗