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Biomedical subjects

T Sudo

Publications and source records attributed to T Sudo.

At least 217 records · Page 12Linked to original sources
PubMed1986

Heterophil Forssman glycoprotein and adenovirus 12: transformed rat cell lines.

Immunobiological significance of Forssman (F) glycoprotein expressed on rat tumor cells derived from transfection of a fibroblast line with whole DNA (WY-3); EcoRI-C fragment, 0-16.5 map units (CY-1); and Accl-H fragment, 0-4.7 map units (HY-1) of adenovirus 12 was investigated. Culturing of F-positive WY-3 and CY-1 cells, but not F-negative HY-1 cells, with monoclonal rat F-antibody resulted in the blocking of their cell-cell adhesion and attachment to plastic surface and inhibition of their growth. Immunization of WY-3 or CY-1 tumor-bearing F344 rats with sheep red blood cells or purified F-antigen in adjuvants brought regression of the tumor in 13 of 19 rats. No such antitumor effect was observed in F-negative HY-1 tumor-bearing rats upon immunization with the F-antigens. Results of this study indicated that the membrane glycoprotein with F-epitope may play a role in lodgement of the tumor cells in vitro and serve as an in vivo target of specific immune effectors.

Animals↗
PubMed1986

Plasma gastrin and secretin changes after esophagectomy.

Plasma gastrin levels were determined in 14 patients with esophageal cancer submitted to an esophagogastrectomy. Six patients were also given 0.1 N hydrochloric acid preoperatively and 1 month after operation to clarify the secretion of plasma secretin. In addition, the correlation between the G cell population in the pyloric part of the postoperative intrathoracic stomach and plasma gastrin was investigated, and the following results were obtained. Hypergastrinemia developed 1 month after surgery, but 3 months or more after surgery, the plasma gastrin level was close to normal. Plasma secretin levels tended to fall postoperatively, but no statistical differences in plasma secretin levels could be found. Staining by the enzyme antibody method revealed hyperplasia of G cells in patients with hypergastrinemia without pyloric antral atrophy.

Aged↗
PubMed1985

A murine cell line (2E10.4.13) produces five hemopoietic stimulators, and interleukin-2 and interleukin-3.

An interleukin-2 (IL-2)-independent murine lymphocyte clone (2E10.4.13) with the Thy1+Lyt1+2-T200+ phenotype was separated from the original IL-2-dependent natural killer (NK) cell line (PEC-1). Erythroid burst-promoting activity (BPA), erythropoietin (Ep), granulocyte/macrophage, megakaryocyte and eosinophil colony-stimulating factors (GM-, MK- and Eo-CSF), IL-2 and Interleukin-3 (IL-3) were produced when these cells were stimulated with phorbol myristate acetate (PMA). When the conditioned medium was run through ion-exchange high-performance liquid chromatography, BPA, Ep, GM-CSF, MK-CSF and Eo-CSF were eluted in the same region as IL-3. In contrast, MK-CSF, much of the GM-CSF and half of the Eo-CSF were eluted in a distinct region where no IL-3 was detected. Chemical analyses of the hemopoietic factors derived from a single T inducer clone indicated that all the hemopoietic activities were associated with IL-3 activity. Some CSF activities (GM-, MK- and Eo-CSF) also could be mediated by the distinct molecules from IL-3, evidence that heterogeneous molecules are responsible for CSF activity.

Animals↗
PubMed1985

[Changes in pancreatic hormones in portal blood and morphological changes of islet cells of Langerhans after hepatectomy].

Hybrid adult dogs weighing 12 to 15 kg were laparotomized before, one week, and one month after partial hepatectomy: Pancreatic tissues were taken and examined by the PAP staining. Also, the animals were intravenously injected via peripheral veins with 50 percent glucose 0.5 g/kg before, one week, and one month after surgery, and their portal blood was taken before, 5, 15, 30, 45 and 60 minutes after injection, and submitted to examination of insulin, glucagon, somatostatin and blood sugar levels. We obtained these results as follows. sigma IRI which represents an increment of insulin secretion after glucose loads, showed a decrease at one week after surgery (p less than 0.05) and an increase at one month after surgery (p less than 0.05). Somatostatin levels of portal blood showed marked increase after surgery (p less than 0.05). Observation on the area of Langerhans islets revealed approximately 4.6 fold hypertrophy from the size of 486 +/- 23 microns m2 before surgery to 2236 +/- 98 microns m2 one month after surgery (p less than 0.05). Insulin secreting B cells were found to contain increased secreting granules. Somatostatin secreting D cells increased 2.8-fold in number one month after surgery, showing mitotic figures.

Animals↗
PubMed1984

Purification and characterization of a tuberculin-active substance from Mycobacterium bovis BCG.

A new tuberculin-active substance, designated TAS-1D3, has been purified from the extract of Mycobacterium bovis BCG by precipitation at pH 4.2, ethanol fractionation, and column chromatography involving CM-cellulose, QAE-Sephadex A-25, Sephadex G-100, and Sephadex G-75. TAS-1D3 was homogeneous in polyacrylamide gel electrophoresis and positive in both Coomassie brilliant blue and periodic acid-Shiff staining, suggesting that TAS-1D3 is a glycoprotein. The molecular weight of TAS-1D3 was estimated to be 26,000 by gel filtration. In amino acid analysis, TAS-1D3 was distinctive in having proline as a dominant amino acid, and in that it lacked basic amino acids, sulfur-containing amino acids and aromatic amino acids. Moreover, TAS-1D3 was almost devoid of absorption at around 280 nm. In guinea pigs sensitized with BCG vaccine, the tuberculin activity of TAS-1D3 was about forty times more potent than that of purified protein derivative (PPD).

Amino Acids↗
PubMed1984

Properties of TAS-1D3, a tuberculin-active substance from BCG, in regard to delayed hypersensitivity.

The properties of TAS-1D3, a tuberculin-active substance purified from the cell extract of Mycobacterium bovis BCG, were studied in vivo and in vitro. In the delayed hypersensitivity skin reaction, TAS-1D3 showed far more potent activity than tuberculin purified protein derivative (PPD) in guinea pigs sensitized with BCG vaccine. This was consistently observed from 6 to 24 weeks after sensitization. The histological findings of the skin reaction to TAS-1D3 were similar to those of the reaction to PPD. Moreover, TAS-1D3 induced well both thymidine incorporation and the production of migration inhibitory factor (MIF) by the spleen cells from guinea pigs sensitized with BCG vaccine. In contrast, TAS-1D3 showed weaker activity than PPD in guinea pigs sensitized with either heat-killed M. tuberculosis Aoyama B or heat-killed M. tuberculosis H37Ra, and it weakly stimulated the spleen cells from animals sensitized with M. tuberculosis Aoyama B to incorporate thymidine and to produce MIF.

Animals↗
PubMed1984

[Teratogenicity study on bromperidol in rats].

A teratogenicity study on bromperidol, a neuroleptic, was carried out in Wistar-Imamichi rats. Bromperidol was administered orally at the dose levels of 0.2, 1.5 and 10.0 mg/kg/day for 11 days from day 7 to day 17 of gestation. About two-thirds of pregnant females in each group were sacrificed on day 21 of gestation, and their fetuses were examined. The remaining dams were allowed to litter naturally, and the post-natal development of the offspring was observed. Treatment of bromperidol caused sedation of pregnant females in 1.5 and 10.0 mg/kg groups with concomitant decrease in body weight and in food and water intake in dose-dependent manner. Effect of bromperidol treatment on F1 generation was observed mainly in animals of 10.0 mg/kg group, suggesting overall growth retardation such as decrease in live fetal weight, retarded ossification of metacarpals and metatarsals, a slight decrease in day 1 viability index, a marginal delay in eyelid opening, reduction in growth rate and decrease in organ weight. No treatment-related external and internal abnormalities were observed in fetuses (F1), and no apparent effect on behavior, learning ability and reproductive function of offspring (F1) was found.

Abnormalities, Drug-Induced↗
PubMed1984

Antitumor activity of deoxyribonucleic acid fraction from Mycobacterium bovis BCG. I. Isolation, physicochemical characterization, and antitumor activity.

A fraction extracted from Mycobacterium bovis strain BCG, which was composed of 70.0% DNA, 28.0% RNA, 1.3% protein, 0.20% glucose, and 0.1% lipid and of no detectable amounts of cell wall components such as alpha, epsilon-diaminopimelic acid and hexosamine, was found to possess strong antitumor activity. Repeated intralesional injection of this fraction, designated MY-1, without attachment to oil or a single intralesional injection of MY-1 emulsified in mineral oil caused the IMC carcinoma of CDF1 mice and line 10 tumor of strain 2 guinea pigs to regress and/or prevented metastasis very effectively. MY-1 after digestion with RNase, which contained 97.0% single-stranded DNA with a guanine-cytosine content of 69.8%, was more effective than undigested MY-1 against IMC and line 10 tumor, while MY-1 digested with DNase, which contained 97.0% RNA, had reduced activity, suggesting that the DNA from BCG possessed strong antitumor activity under certain conditions. Details of the extraction procedures and physicochemical characterization of MY-1 were also described.

Animals↗
PubMed1984

Expression of heterophil Forssman antigen as glycoprotein on transformed rat cell lines: shedding of the antigen from the cells.

Monoclonal Forssman (F) antibodies of the IgM class were obtained by hybridization of spleen cells from an immunized F344 rat with murine myeloma cells. By means of the indirect immunofluorescence test with monoclonal F-antibodies, F-antigen was demonstrated on rat cell lines derived from a normal cell line that was transformed by transfection with whole adenovirus type 12 DNA or a fragment (E1a + E1b) of adenovirus type 12 DNA. These transformed cells were shown to shed the F-antigen into the culture supernatant, depending on their degree of malignancy. The F-antigen was demonstrated in a glycoprotein, but not in a glycolipid fraction of the supernatants. The glycoprotein purified by affinity chromatography was subjected to gel electrophoresis and subsequent Western blotting. The F-active molecules were identified as three distinct bands of approximately 130,000, 60,000, and 27,000.

Adenoviridae↗
PubMed1984

[Plasma gastrin and secretin levels in patients with carcinoma of the esophagus before and after operation].

Twenty-two patients with esophageal cancer (8: preoperative, 8: one-month after surgery, and 6: more than 3 months after surgery) were orally loaded with 50 g of glucose, to determine the plasma gastrin levels. They were also loaded with oral 0.1N hydrochloride preoperatively and also one month after operation to examine the secretion kinetics of plasma secretin. In addition, the correlation between the G-cell (gastrin secretory cell) population in the pyloric region of the postoperative intrathoracic stomach and the plasma gastrin was investigated. The following results were obtained. Hypergastrinemia developed one month after surgery, but after 3 months or later, plasma gastrin levels almost returned to normal. Plasma secretin levels tended to decrease postoperatively; however, no statistically significant differences were observed. G-cell stain by the enzyme antibody method revealed hyperplasia of G-cells in some of the hypergastrinemic cases without accompanying atrophy of the pyloric mucosa and/or the intestinal epithelial metaplasia, although there were some hypergastrinemic cases with a decrease of G-cells due to the atrophy of the pyloric mucosa and/or the intestinal epithelial metaplasia. In the latter cases, gastrin was thought to be secreted probably from the duodenal mucosa.

Aged↗
PubMed1984

Changes in plasma gastrin and secretin levels after pancreaticoduodenectomy.

The kinetics of secretion of gastrin and secretin before and after pancreaticoduodenectomy were studied. Prior to pancreaticoduodenectomy, gastrin secretory function was within the normal range; it was significantly reduced postoperatively. Preoperatively, patients had elevated basal levels of secretin with depressed reactivity. During pancreaticoduodenectomy, the upper part of the jejunum should be preserved as far as possible. Ulcers occurring after pancreaticoduodenectomy are more probably due to vagus nerve activity and other factors rather than to an imbalance between gastrin and secretin.

Adult↗