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Biomedical subjects

T Sudo

Publications and source records attributed to T Sudo.

At least 199 records · Page 11Linked to original sources
PubMed1988

[Clinical studies on various therapy for the intractable trauma of toes and fingers in cases of diabetes mellitus and peripheral ischemic diseases].

In cases of ischemic extremities and diabetes mellitus, the trauma on finger and toe is very intractable. For such injuries amputation of extremity is indicated very often because of severe necrosis. The number of such cases has been increasing recently because many cases of these patients have arteriosclerotic arterial occlusion and diabetes mellitus, and these are correlated with the changes of aging. The number of cases of Buerger's disease has been also increasing and it is another etiology of intractable trauma in ischemic extremity. The repeated hyperbaric oxygenation, sympathetic block, warfarin therapy and insulin bath with bubbling of hyperbaric oxygen, were applied to has been of such necrosis. By these procedures, the rate of amputation of extremity decreasing. It was concluded that the surgical reconstruction of artery for ischemic extremity has never any meaning as the therapy of such intractable injuries, if blood flow in the peripheral tissue is not kept physiologically, before vascular reconstruction. In order to increase peripheral tissue circulation, the hyperbaric oxygenation, sympathetic block and warfarin therapy wer performed in many cases and these methods were very effective for intractable injuries with severe necrosis.

Adult↗
PubMed1987

Generation of continuous large granular lymphocyte lines by interleukin 2 from the spleen cells of mice infected with Moloney leukemia virus. Involvement of interleukin 3.

Continuous cell lines could be reproducibly established by culturing spleen cells from adult mice injected with MLV-producer cells or directly infected with Mo-MLV with rIL-2, whereas the culture of normal splenic cells with rIL-2 induced only transient and limited proliferation resulting in no such lines. All of the lines showed morphological characteristics as LGL with Thy-1+,Lyt-1-,L3T4-,Lyt-2-,AsGM1+,FcR gamma+ phenotype without exception, and most of them exhibited typical NK-patterned cytotoxicity. Analysis of reverse transcriptase activity of the culture supernatants as well as Southern hybridization of the DNA from the lines using an Mo-MLV-specific cDNA probe indicated no evidence of retroviral replication or proviral integration, suggesting that the generation of cell lines reflected a reactive process and viral infection was not directly responsible. It was subsequently revealed that Thy-1+,Lyt-1+,Lyt-2- spleen cells from mice infected with Mo-MLV in vivo spontaneously produced surprising amounts of IL-3 in vitro, leading to the possibility that IL-3 was responsible for the generation of lines. The possibility was directly supported by the observation that continuous lines with identical characteristics could be generated completely in vitro by sequential stimulation with rIL-3 and rIL-2 from normal spleen cells without any involvement of Mo-MLV. The C beta gene of TCR was shown to be rearranged in all the lines examined, indicating the LGL lines were all genetically committed to T cell lineage. Unlike the situation in normal splenic populations expanded by rIL-2, where the expression of IL-2-R was progressively lost, constitutive expression of high-affinity-IL-2-R was observed in all the lines and thus, this was considered to explain the unlimited proliferation of them in response to rIL-2 alone. These results suggested the probable role of IL-3 in the regulation of growth and differentiation of a set of LGL committed to T cell lineage. The possible implications of the phenomenon in the regulation of hematopoiesis as well as in the control of Mo-MLV-induced leukemogenesis were discussed.

Animals↗
PubMed1987

Study of calcium phosphate crystalluria.

When the frequency of heavy calcium phosphate crystalluria was compared among postprandial urine specimens collected from 152 healthy persons (group 1) and 108 patients with calcium urolithiasis (group 2), there was no significant difference between these groups. However, the frequency of crystalluria was significantly greater in 49 patients in group 2 who required or had required surgical intervention for calcium stone disease than in group 1 (p less than 0.05). Calcium phosphate precipitates collected from 7 urine specimens by repeated centrifugation were studied by infrared, X-ray, thermal and electron probe analyses. The principal component of the precipitates was poorly crystallized apatite associated with some organic material. The precipitates from the 7 samples appeared as aggregates of fine spherules less than 1 micron. in diameter, and the calcium-to-phosphate molar ratio of a spherule ranged from 1.02 to 1.19. We conclude that not only calcium oxalate crystalluria but calcium phosphate crystalluria may have an important role in the formation of urinary stones.

Adult↗
PubMed1987

Inhibitory effects of tunicamycin on the mixed lymphocyte reaction and mitogen-induced lymphocyte blastogenesis.

The role of asparagine(Asn)-linked saccharides on the surface of lymphocytes in cellular interactions was examined by performing studies on the effects of tunicamycin (TM), which inhibits the glycosylation of proteins N-glycosylated at asparagine residues, on the mixed lymphocyte reaction (MLR) and mitogen-induced lymphocyte blastogenesis of human lymphocytes by measuring 3H-thymidine (TdR) incorporation. Responses were expressed as percentages of that of the control (MLR without TM). The lymphocyte blastogenesis in the one- and two-way MLR were, respectively, 43.1% and 48.0% of the control at 0.1 microgram/ml of TM, and 5.5% and 7.2% at 1 microgram/ml of TM. The inhibitory effect of TM on the one-way MLR was shown using TM-pretreated stimulator cells, TM-pretreated responder cells or both. TM blocked lymphocyte blastogenesis in the secondary as well as in the primary MLR. The inhibitory effect of TM on the two-way MLR was observed when TM was added on day 0 to day 2, but not on day 4 of incubation. TM blocked mitogen-induced lymphocyte blastogenesis by phytohemagglutinin, concanavalin A or by pokeweed mitogen. As TM had no cytotoxic effect on cultured cells, these inhibitory effects of TM were thought to be due to the loss of Asn-linked saccharides from glycoprotein of the surface of lymphocytes. These findings indicated that Asn-linked saccharides of glycoprotein on the surface of lymphocytes were important in cellular interactions that are necessary for the cellular immune response.

Antigens, Surface↗
PubMed1987

Nude mice bearing human CSF-producing tumor: analysis of hemopoietic factor(s) acting on primitive stem cells.

Previously, we serially transplanted tumors that produced colony-stimulating factor (CSF) into nude mice, who developed marked granulocytosis along with tumor growth; their leukocyte counts reaching approximately one million per cu mm. The numbers of CFU-GM, CFU-E, CFU-Meg, CFU-S and BFU-E were increased in nude mice bearing CSF-producing tumor. We here report that tumor-conditioned medium (TCM) derived from the CSF-producing tumors had colony-stimulating activity (CSA) and burst-promoting activity (BPA) when normal murine spleen cells as well as normal human bone marrow cells were the target cells. The activity of TCM supported multilineage colony formation in 5-fluorouracil (5-FU)-treated mouse spleen cells, in which only the primitive population of stem cells was reserved. No interleukin-3 (IL-3) activity was detected in TCM when assayed using the IL-3 dependent cell lines. We conclude that the factor in TCM acts on pluripotent stem cells and on the early progenitor stage of various cell lineages. It is distinct from IL-3.

Animals↗
PubMed1986

A recombinant murine granulocyte/macrophage (GM) colony-stimulating factor derived from an inducer T cell line (IH5.5). Functional restriction to GM progenitor cells.

The cDNA for the murine granulocyte/macrophage colony-stimulating factor (GM-CSF) was cloned from a cDNA library obtained from a murine T cell line, IH5.5, by using two synthetic probes that encoded two parts of the GM-CSF from murine lung. The cDNA inserted into the plasmid vector pcDV1 was transfected into monkey COS-1 cells and the conditioned medium was used to investigate the hemopoietic activities of the resultant product, recombinant GM-CSF (rGM-CSF), by means of various colony assays. rGM-CSF stimulated only neutrophil/macrophage colonies in the cultures of murine normal bone marrow and fetal liver cells. No other colony stimulating activities (CSA) were seen in the preparation including burst-promoting activity, eosinophil-CSA, megakaryocyte-CSA and mast cell-CSA. rGM-CSF could not support colony formation of 5-fluorouracil-treated mouse spleen cells, in which only the primitive population of stem cells survived. However, after culture of these cells with PWM-spleen cell-conditioned medium (PWM-SCM), the colonies consisting of blast cells were formed. These blast cells could now be induced to form neutrophil/macrophage colonies in the presence of rGM-CSF. Pure neutrophil colonies, pure macrophage colonies, as well as mixed neutrophil/macrophage colonies, were formed from these single blast cells in the presence of rGM-CSF by micromanipulation. rGM-CSF did not act on pluripotent hemopoietic stem cells, but did act directly and selectively on neutrophil/macrophage progenitors. Moreover, striking heterogeneities were noted in the size of the colonies and the proportion of components. GM-CSF is, therefore, considered to play a noninstructive role in the differentiation of the GM pathway.

Animals↗
PubMed1986

[Structural and hormonal alterations in the pancreas after hepatectona in dogs].

Humoral factors in the portal blood have often been suggested to play an important role in hepatic regeneration after hepatectomy. However, the correlation among insulin, glucagon and somatostatin, and islet cells of Langerhans has not been studied. Forty percent hepatectomy was carried out in dogs and intravenous glucose administration (0.5 g/kg) was the function of the pancreas to release one week after and one month after surgery to estimate administered intravenously before surgery, the plasma insulin, glucagon and somatostatin. In addition, islet cells of Langerhans were investigated with PAP technique. The following results were obtained. Alterations of carbohydrate metabolism developed as a result of portal plasma insulin deficiency one week after surgery, but granules in B cells were demonstrated as much as preoperatively. It was suggested that hypersomatostatinemia suppressed insulin release from B cells. Alterations of carbohydrate metabolism was restored to normal and portal plasma insulin response was increased one month after surgery. These indicate that hepatic regeneration after hepatectomy may contribute to these changes. Islets cell of Langerhans were hypertrophic and particularly, D cells were increased after surgery.

Animals↗
PubMed1986

Immunohistochemical changes of the islet cells of Langerhans after partial hepatectomy in dogs.

Morphological changes were studied in the islet cells of Langerhans after hepatic resection in dogs. The animals were laparotomized before and 1 wk and 1 month after partial hepatectomy. Pancreatic tissue was taken and immediately immersed in neutral formalin and then stained with PAP. One hundred visual fields were examined microscopically. One month postoperatively there was 4- to 5-fold hypertrophy of the islets of Langerhans (486 +/- 23 micrometers 2 before to 2236 +/- 98 micrometers 2 1 month after operation) (p less than 0.01). A, B, and D cells were also found to be increased in number. D cells, especially, showed mitotic division and proliferation, and the ratio of the number of D cells pre-, 1 wk post-, and 1 month postoperation was 1:1.37:2.80, showing a gradual increase after operation.

Animals↗