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Biomedical subjects

T Shida

Publications and source records attributed to T Shida.

At least 271 records · Page 15Linked to original sources
PubMed1981

[Immunopharmacological actions of neurotropin. (2). Effect of neurotropin on graft-versus-host reaction (author's transl)].

It was reported that Neurotropin (NSP) is a new immunostimulating agent which reverses the decrease of proliferation of irradiated spleen cells from mice caused by concanavalin A or specific antigen. The present study was undertaken to examine the possibility that NSP prevents graft-versus-host reaction (GVHR) through host-versus-graft reaction (HVGR) in mice. Allogeneic (C3H/He) spleen cells were performed intravenously into 900 R-irradiated recipient mice (BALB/c). Systemic GVHR was clearly prevented by NSP as well as by cyclophosphamide or prednisolone administered intraperitoneally into irradiated recipient mice. In recipient mice irradiated with 1000 R, a more remarkably systemic GVHR was induced as compared to recipients irradiated at 250 to 500 R. NSP suppressed the proliferation of donor cells at the induction phase of GVHR, whereas NSP did not inhibit both systemic and local GVHRs induced by cell transfer at the effector phase. A decrease in hematopoietic function following irradiation or dosing with immunosuppressive drugs was also reversed by NSP. However, NSP had no effect on increases in hematopoietic function mediated by syngeneic bone marrow transplantation into 900 R-irradiated mice. These results strongly suggest that the inhibition of allogeneic GVHR by NSP is responsible for the suppression of donor cell proliferation by adequately restoring immunologic function in irradiated recipient mice, namely HVGR.

Adjuvants, Immunologic↗
PubMed1980

Simultaneous determination of histamine and N tau-methylhistamine in human plasma and urine by gas chromatography--mass spectrometry.

A new and sensitive method is described for the determination of histamine and N tau-methylhistamine in human plasma and urine by gas chromatography--mass spectrometry. 15N2-Labeled histamine and N tau-[methyl-d3]methylhistamine were used as internal standards. Histamine and N tau-methylhistamine were converted to the derivatives N alpha-heptafluorobutyryl-N tau-ethoxycarbonylhistamine and N alpha-heptafluorobutyryl-N tau-methylhistamine, respectively. After these derivatives had been purified on a small column packed with CPG-10, the molecular ions were monitored during selected ion monitoring. Linear standard curves were obtained in the range of 0.5-10 ng/ml for both compounds. The reliability of the histamine analysis was demonstrated by using two different ion pairs, while a comparison with results from two different derivatizations on the same urine sample also established the specificity of the N tau-methylhistamine analysis. An increase of 1 ng of histamine in the plasma could be precisely determined by the present method. The histamine content of plasma from five normal subjects was determined as 0.83 +/- 0.37 (S.D.) ng/ml and the N tau-methylhistamine content in most subjects was below the limits of this measurement. High excretion of histamine was noted in the urine collected in the early morning from a patient with nephritis.

Gas Chromatography-Mass Spectrometry↗
PubMed1980

Clinical evaluation of a new orally active anti-anaphylactic compound: ketotifen (HC 20-511) in Japanese adult asthmatics.

The protective effect of ketotifen was examined by an open assessment trial in 261 asthmatics of variable types, a double blind trial comparing ketotifen and inert placebo in 189 asthmatics of extrinsic type including pure atopic asthma and asthma with atopic and infectious components (mixed type or combined type), and a double blind trial comparing ketotifen and disodium cromoglycate (DSCG) by double placebo technique in 132 asthmatics of a pure atopic type. The open clinical assessment trial suggested that ketotifen was most effective in atopic asthmatics having moderate and severe as well as mild asthmatic episodes. Efficacy increased with prolonged administration; 1 mg twice daily was better than a 1 mg daily dose. In the placebo-controlled double blind trial, the efficacy of ketotifen was demonstrated by the reduction of concomitant anti-asthmatic therapy. The results of the double blind trial comparing ketotifen and DSCG are not fully reported. However, preliminary data showed that the efficacy of both drugs was equivalent or that ketotifen was slightly superior to DSCG. Safety of ketotifen was confirmed by the three trials, transient day-time sedation being the major side effect of this agent. In conclusion, ketotifen is a promising drug for the prophylaxis of asthma.

Adult↗
PubMed1980

Quantitative analysis of histamine in biological samples by gas chromatography--mass spectrometry.

A mass fragmentographic method for the quantitative analysis of histamine in the supernatant from antigen-challenged leukocytes, whole blood, and urine is described. Histamine labeled with two 15N atoms was synthesized and added to the sample as an internal standard. N alpha-Heptafluorobutyryl-N tau-ethoxycarbonylhistamine was prepared for mass fragmentographic analysis and the molecular ions at m/z 379 and 381 were used for monitoring histamine and 15N2-labeled histamine, respectively. The quantitation limit of histamine was 2 ng by this method. The experimental error of the method was less than 7% at the level of 5 ng in the supernatant from antigen-challenged leukocytes. The value obtained by this method correlated well with that from radioisotopic enzymatic assay (r=0.990).

Gas Chromatography-Mass Spectrometry↗
PubMed1980

Interactions between oligonucleotides having a left-handed helical structure and ethidium bromide.

Oligonucleotides containing a 8,2'-S-cycloadenosine(As), 8,2'-S-cycloinosine(Is), 6,2'-O-cyclouridine(Uo) and 6,2'-O-cyclocytidine(Co) residues, which have a glycosidic torsion angle(chi) of about 120 degrees, were synthesized. Among these oligomers, AspUo, AspIs and (pCo)4 + (pIs)4 formed a complex with ethidium bromide, which was assumed to be intercalated between the adjacent base-pairs of the left-handed double helix.

Chemical Phenomena↗
PubMed1978

Gold salt in the treatment of bronchial asthma--a double-blind study.

The efficacy of gold salt on bronchial asthma was investigated by double-blind design in 79 patients. The rate of improvement was significantly higher in the gold-treated group (20/28) than in the controls (16/36). This was reflected by greater reduction of requirement for symptomatic medications. Only mild side effects were observed in 14 patients out of 38 gold-treated patients.

Asthma↗
PubMed1976

Surgical treatment of tricuspid insufficiency in patients undergoing mitral valve replacement.

During the past eight years, 46 of the 106 patients who underwent mitral valve replacement were associated with tricuspid insufficiency. No surgical correction was performed (14 cases) in cases of slight tricuspid insufficiency. Tricuspid annuloplasty (11 cases) or valve replacement (21 cases) was employed according to the severity of insufficiency. In the non-repair group, the mortality rate was fairly low (21 per cent), but the postoperative status was the least satisfactory by the NYHA classification. Tricuspid insufficiency was significantly reduced only in two of these 14 cases after the mitral valve replacement. In the tricuspid annuloplasty group, although the technique of tricuspid annuloplasty did not always correct insufficiency completely, only one patient died of residual insufficiency. The cardiac output measured with Minnesota Impedance Cardiograph increased postoperatively in proportion to stress in this group. In the tricuspid valve replacement group, cardiac catheterization studies revealed hemodynamic improvement at rest in all, but cardiac output during exercise remained unchanged or decreased in some cases. Now we consider that tricuspid insufficiency with advanced mitral valve disease, even of a slight degree, should be surgically treated and that annuloplasty has more obvious hemodynamic benefits than valve replacement.

Adult↗