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Biomedical subjects

T Shida

Publications and source records attributed to T Shida.

At least 253 records · Page 14Linked to original sources
PubMed1982

Synthesis and properties of CpG analogues containing an 8-bromoguanosine residue. Evidence for Z-RNA duplex formation.

Three dinucleoside monophosphates containing 8-bromoguanosine (br8G), (2'-5')C-br8G, (3'-5')C-br8G, and dC-br8G, were synthesized and characterized by UV absorption, CD, and 1H NMR spectroscopy. The 1H NMR data show that all the br8G residues in these dimers take a syn glycosidic conformation. At low dimer strand concentration (5 X 10(-5) M), the UV hypochromicity data suggest that the degree of base stacking decreases in the following order, (2'-5')C-br8G greater than C-G approximately equal to dC-br8G greater than (3'-5')C-br8G. The CD data also suggest little stacking in (3'-5')C-br8G. At high dimer strand concentration (5 X 10(-3) M), only (3'-5')C-br8G shows duplex formation in 0.1 M NaCl. The duplex is assumed to take a left-handed helical structure similar to that of Z-DNA. The Tm of this duplex is surprisingly high for a dimer (about 35 and 45 degrees C at 5 X 10(-3) and 10(-2) M dimer strand concentration, respectively). The above results and the similarity between the CD spectra of (3'-5')C-br8G and poly(G-C) suggest the possible existence of Z-form structure in ribooligo- and ribopolynucleotides with alternating purine-pyrimidine sequences.

Circular Dichroism↗
PubMed1982

Isolation and chemical properties of a haptenic substance from buckwheat dialysate.

A haptenic substance was isolated from the dialysate of the aqueous extract of buckwheat. This substance, BWD II 22-3, which was composed of Asp(1), Thr(1), Ser(1), Gly(1), Gly(4), Ala(1), Val(1), Leu(1), Orn(2), Lys(1), Arg(1), Cys(1) and glucose was demonstrated to be homogeneous by gel filtration and paper electrophoretic analyses. The molecular weight was estimated to be 1600 by gel filtration on Sephadex G-50. The haptenic substance apparently has-SH group determinant caused about 50% inhibition at a concentration of 100 micrograms/disc in the RAST procedure using human serum sensitive to buckwheat. An active component, BWD II 22-3, might prove effective in the hyposensitization therapy of buckwheat-sensitive patients.

Desensitization, Immunologic↗
PubMed1982

Pulmonary varices associated with mitral valve disease: a case report and survey of the literature.

A case of pulmonary varices in a 24-year-old man with mitral regurgitation is reported. A brief summary of the literature on pulmonary varices associated with mitral valve disease is presented, with special reference to its etiology and surgical treatment. It is strongly suggested that production of varicosities of the pulmonary vein requires not only increased pulmonary venous pressure but also other local factors. If the patient has symptoms due to mitral regurgitation, operation for mitral valve disease is indicated. Direct operative intervention for the varices should not be performed.

Adult↗
PubMed1982

Effect of Bordetella pertussis on alpha 1 and beta-adrenergic and cholinergic muscarinic receptors in guinea pig lung membranes.

After intraperitoneal injection of Bordetella pertussis vaccine to guinea pigs, the alpha 1- and beta-adrenergic and cholinergic muscarinic receptors of the whole lung were measured by binding assays with the radioisotope-labeled antagonists, 3H-prazosin, 1-(3)H-dihydroalprenolol and 1-(3)H-quinuclidinyl benzilate, respectively. Injection of guinea pigs with pertussis vaccine resulted in an increase in the maximum binding (Bmax) of 3H-prazosin, while there was about a 30% reduction in 1-(3)H-dihydroalprenolol binding to beta-adrenergic receptors. No difference was observed in the dissociation constant (KD) for binding of each ligand to alpha 1- and beta-adrenergic receptors. 1-(3)H-quinuclidinyl benzilate binding indicated that the Bmax and KD for cholinergic muscarinic receptor in pertussis-sensitized guinea pigs were not significantly different from those normal control animals.

Animals↗
PubMed1982

Deoxyoligonucleotide chain cleavage reactions by phenanthroline and bleomycin.

To elucidate the mechanisms of double-stranded DNA chain cleavage by 1,10-phenanthroline-Cu(I) and bleomycin-Fe(II) complexes in oxygen-dependent reactions, self-complementary deoxyoligonucleotides were treated with the complexes and the degradation products were characterized. The degradation products of d(C-G) by the phenanthroline complex were fully identified by UV, proton and carbon-13 NMR and paper electrophoresis. It is assumed that the DNA chain cleavage and concomitant base release are caused by oxidation of Cl' of the deoxyribose moiety. A duplex of d(ATACGCGTAT) was cleaved by both complexes. Bleomycin shows some sequence specificity but phenanthroline does not.

Base Sequence↗
PubMed1982

The effect of local blood flow on the healing of experimental intestinal anastomoses.

Using dogs, we experimentally produced devascularized areas in the small intestine and colon and took quantitative measurements of the submucosal local blood flow using the hydrogen clearance method.. In the area slightly toward the ischemic side of the devascularized margin, local blood flow is less on the mesenteric side in both the ileum and the colon, and microcirculation is better maintained in the colon that in the ileum. When the anastomosis was performed in the area in which the local blood flow on the mesenteric side was 60 per cent of the control value in the ileum or 70 per cent in the colon, recovery of the control value was achieved by the seventh postoperative day. When anastomosis was performed in the area in which the local blood flow on the mesenteric side was 15 per cent of the control value in the ileum and 22 per cent of the control value in the colon, the flow on the mesenteric side did not recover to the level of the control value by the seventh postoperative day, and anastomotic leakage occurred in some dogs. All anastomotic leakage occurred on the mesenteric side. The local blood flow rate at the point at which the last signal was detected by Doppler ultrasound flowmeter after moving Doppler probe from the devascularized margin toward the ischemic side was 57 per cent of the control value in the ileum and 54 per cent of that in the colon. Such levels of blood flow are considered sufficient for successful healing of tissue after an anastomosis.

Animals↗
PubMed1981

Left handed double helices: effect of sequence on the spatial configuration of high anti nucleic acids.

The conformational properties of purine-pyrimidine and pyrimidine-purine dinucleoside monophosphates in which the glycosidic torsion is fixed to congruent to 120 degree by the formation of a covalent link between the base and the sugar ring are explored by 1H NMR spectroscopy in order to obtain information about the spatial configuration of high anti nucleic acids. The intramolecular stack of the high anti dimers were found to be left handed, in contrast to that (right handed) for natural oligomers, which are low anti. Even though both the high anti pyrimidine-purine and purine-pyrimidine dimers have similar backbone torsion angles, they display widely different relative geometry between the bases; thus in the former there is extensive base-base overlap in the stack, and in the latter there is negligible intramolecular base-base overlap. In addition it was found that purine-pyrimidine systems form miniature double helices in which there is substantial interstrand purine-purine interaction; on the other hand the pyridine-purine high anti dinucleosides have no proclivity to form such base-paired complexes in solution. Mathematical polymerization of the conformation of the high anti purine-pyrimidine dinucleoside monophosphates generates a left handed helix for high anti polynucleotides. This also means that the double helix for high anti-nucleic acids containing purine-pyrimidine repeated units may also be left handed, as had been suggested [Sundaralingam, M., & Yathindra, N. (1977) Int. J. Quantum Chem., Quantum Biol. Symp. 4, 285]. It is suggested that the plasticity in the structure of genomic DNA is such that, if under certain conditions of interactions the sugar-base torsion of certain domains assume high anti values, that domain will become left handed, and this in turn can be a mechanism for the control of expression by genomic DNA.

Dinucleoside Phosphates↗
PubMed1981

Relationship between the results of skin, conjunctival and bronchial tests and RAST with Candida albicans in patients with asthma.

The results of the skin, conjunctival and inhalation tests and RAST with Candida antigen were compared with each other in patients with bronchial asthma. The correlation coefficient between the skin test and RAST was 0.50 (P less than 0.01). The correlation coefficient between the skin test, RAST and the inhalation tests was 0.36 (P less than 0.01) and 0.57 (P less than 0.01). The positive agreement of the inhalation test to the skin test, RAST and the conjunctival test was 77, 86 and 93% respectively. In this study the conjunctival test was thought to be the best method in place of the inhalation test. There was no significant relation between the serum IgE concentration and the endpoint of the skin titration testing, and a low correlation was noticed between the serum IgE concentration and RAST counts (r = 0.36).

Adolescent↗
PubMed1981

Anti-mite IgE antibody production in vitro by peripheral blood lymphocytes from mite-sensitive atopic patients.

Anti-mite IgE antibody production in vitro was investigated using peripheral blood lymphocytes (PBL) from mite-sensitive patients with bronchial asthma. Preculture of PBL with the mite antigen, which induced the remarkable cell proliferation, resulted in a marked decrease of the IgE antibody production directed to mite but not to unrelated antigens. Anti-mite IgE antibody formation was not inhibited by autologous or allogeneic T cells stimulated with the mite antigen. The spontaneous production of IgE antibody was observed when fractionated B cells were cultured alone or with pokeweed mitogen (PWM), but was suppressed when they were precultured with the antigen. Coculture of autologous T cells with B cells showed a considerable augmentation of anti-mite IgE antibody formation. The enhancing activity of autologous T cells was not observed when B cells were irradiated at 1,000 rad. The spontaneous IgE antibody production by B cells alone was radioresistant. Both mitotic inhibitors and 1,000 to 2,000 rad of gama-irradiation did not significantly suppress the spontaneous anti-mite IgE antibody production by B cells.

Antigens↗
PubMed1981

[Immunopharmacological actions of neurotropin. (1). Immunostimulating actions of neurotropin (author's transl)].

Neurotropin (NSP) is an extract isolated from vaccinia virus-inoculated and inflamed skin of rabbits. The present study was undertaken to examine the immunostimulating actions of NSP. NSP alone did not induce a proliferation of spleen cells from mice or human peripheral blood lymphocytes (PBL). However, NSP clearly enhanced concanavalin A (Con A)-induced proliferation of both spleen cells and human PBL, but slightly inhibited lipopolysaccharide (LPS)-induced proliferation of spleen cells. NSP reversed the decreases of Con A-induced proliferation of spleen cells pretreated with mitomycin C (MMC) in a dose of 5 microgram/ml or irradiated at 100 to 500 R, while NSP did not reverse the decreases of LPS-induced proliferation of spleen cells pretreated with MMC, in a dose of 5 to 50 microgram/ml, or irradiated at 100 to 1000 R. NSP enhanced in a dose-dependent fashion one-way mixed lymphocytes culture (MLC) reaction using MMC-treated spleen cells from BDF1 mice as stimulator cells and those from C57BL/6 mice as responder cells, respectively. NSP reversed the considerable decrease of MLC-mediated proliferation of responder cells pretreated with MMC, in a dose of 5 to 10 microgram/ml, or irradiated at 100 to 250 R. These results indicate that NSP activates T cell function participating in cell-mediated immunity.

Adjuvants, Immunologic↗
PubMed1981

[Immunopharmacological actions of neurotropin (3): Anti-allergic actions of neurotropin (author's transl)].

Neurotropin (NSP) is an extract isolated from vaccinia virus-inoculated and inflamed skin of rabbits. The anti-allergic actions of NSP were studied in the present paper. Death from anaphylactic shock in BALB/c mice mediated by active systemic anaphylaxis (ASA) or heterologous passive systemic anaphylaxis (PSA) was completely prevented in one of five cases by NSP given 5000 micrograms/animal i.v.. However in mice, NSP at a dose of 10 to 1000 micrograms/animal i.v. did not have any preventive effect on death from anaphylactic shock induced by ASA, homologous PSA, or heterologous PSA. NSP slightly inhibited histamine release from rat mast cells mediated by mouse IgE antibody, calcium ionophore A23187, or compound 48/80, but this inhibition was not in a dose-dependent fashion. NSP did not behave synergistically with isoproterenol- or theophylline- induced inhibition of mouse IgE antibody-mediated histamine release from rat mast cells. NSP did not inhibit D2O-enhanced histamine release from sensitized rat mast cells. NSP clearly inhibited antigen-induced histamine release from leukocytes of mite-sensitive patients with or without asthmatic attack. Potent inhibition was observed when leukocytes taken from patients with asthmatic attack were used. These results suggest that the anti-allergic action of NSP is not responsible for elevation of cyclic AMP levels or disruption of microtubules in target cells.

Anaphylaxis↗