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Biomedical subjects

T Sano

Publications and source records attributed to T Sano.

At least 757 records · Page 42Linked to original sources
PubMed1982

Immunohistochemical observation of intracytoplasmic lysozyme in proliferative and neoplastic fibrohistiocytic lesions.

Distribution of intracytoplasmic lysozyme in proliferative and neoplastic fibrohistiocytic lesions and non fibrohistiocytic tumors was studied by immunoperoxidase technique on formalin fixed, paraffin-embedded sections. The cases examined were 161 fibrohistiocytic lesions and 86 non-fibrohistiocytic tumors. Contrary to our expectation, the lysozyme positive cells were found only in the minority of cases with fibrohistiocytic lesions. Cells positive for lysozyme were found only in 13 out of 100 cases of dermatofibroma, one out of 4 cases of xanthogranuloma and 8 out of 33 cases of malignant fibrous histiocytoma. Dermatofibrosarcoma protuberans and non-fibrohistiocytic tumors were negative for lysozyme. It is suggested that in proliferative fibrohistiocytic lesions, induction of lysozyme synthesis is weak or absent. Some malignant fibrous histiocytomas showed scattered lysozyme positive neoplastic cells, indicating their probable histiocytic origin or differentiation. On the other hand, evidence of histiocytic differentiation of dermatofibrosarcoma protuberans was not obtained using lysozyme immunohistiochemistry.

Aged↗
PubMed1982

Mixed connective tissue disease with fatal pulmonary hypertension.

An autopsy case of mixed connective tissue disease (MCTD) with pulmonary hypertension is presented. A 34-year-old woman suffering from arthralgia, Raynaud's phenomenon, and dyspnea of 6-years duration was diagnosed as having MCTD on the basis of a high titer (1:160,000) of serum antibody to the ribonuclease-sensitive component of extractable nuclear antigen. Examination of cardiac function revealed the complication of pulmonary hypertension. Autopsy revealed concentric intimal cellular proliferation of the small arteries and arterioles of both lungs. Typical plexiform lesions of these vessels were also observed. These findings coincide with those of plexogenic pulmonary angiopathy of primary pulmonary hypertension (PPH). This is the second autopsy case of MCTD with fatal pulmonary hypertension reported and our observations suggest that some cases with PPH who had immunological abnormalities but could not be classified as cases of classical collagen disease, may have been induced by MCTD.

Adult↗
PubMed1982

Electron microscopical study on impaired phagocytic function of granulocytes in diabetics.

Phagocytic ability of granulocytes obtained from 22 diabetic patients was studied by electron microscopy in order to explain their susceptibility to infection and was compared with that of granulocytes from 13 healthy controls. The main features of the procedure employed in this study were as follows; (1) morphologically quantitative assessment of phagocytosis, (2) no separate assay of engulfment and killing of bacteria, (3) granulocytes were mixed with 10 times their number of bacteria, (4) antibiotics were not used to kill extracellular bacteria, (5) use of a conic tube lined with collodion film for cell pellet formation, (6) each conic tube contained less than 1 X 10(6) granulocytes, (7) sections examined were the vertically cut surfaces of the thin buffy coat. Control subjects showed that most granulocytes did not contain intact intracellular bacteria. Assay of aliquots sampled after 30 minutes and 2 hours in controls showed no significant difference. However, in diabetics, eight cases showed similar results with controls and were thought to have normal function, and fourteen cases showed the increased number of granulocytes containing intact intracellular bacteria was much more marked in aliquots sampled after 2 hours and were considered as showing various degrees of impaired bactericidal ability.

Adult↗
PubMed1982

Demonstration of two distinct antigenic determinants on hepatitis B e antigen by monoclonal antibodies.

Mice were immunized against hepatitis B e antigen (HBeAg) isolated from sera of asymptomatic carriers of hepatitis B virus. Their spleen cells were fused with mouse myeloma (NS-1) cells, and 5 clones of hybridoma cells secreting antibody against HBeAg (anti-HBe) were isolated. For the production of anti-HBe in large scale, cells were cultivated both in vitro and in the peritoneal cavity of ascitic mice. Although monoclonal antibodies produced by these clones showed a strong reactivity of anti-HBe in hemagglutination tests, individual monoclonal anti-HBe did not reveal any precipitin line in immunodiffusion. When 2 of the 5 monoclonal antibodies were mixed together, however, some combinations showed a precipitin line against HBeAg, whereas others did not. Utilizing solid-phase radioimmunoassay involving a number of combinations of monoclonal antibodies used for solid-phase and radiolabeling, the 5 antibodies were classified into 2 groups. Three of the anti-HBe antibodies were found to be directed to 1 determinant of HBeAg (determinant a); the remaining 2 to the other determinant (determinant b). Determinants a and b were detected on HBeAg in the serum, as well as on the polypeptide of 19,000 daltons (P19) derived from the nucleocapsid of hepatitis B virus. Monoclonal anti-HBe antibodies with different specificities may provide useful tools in delineating the antigenic structure of HBeAg and also in evaluating immune responses of the host directed to its subdeterminants.

Animals↗
PubMed1982

Potentiation of natural killer cell activity of human lymphocytes in vitro: the participation of interferon in stimulation with Staphylococcus aureus Cowan I bacteria but not with protein A.

In the previous paper we reported that human natural killer (NK) cell activity was augmented greatly by preincubation with Staphylococcus aureus Cowan I bacteria (SpA CoI) or its Protein A. We examined here whether the augmentation with these stimulants is ascribable to the direct activation of NK cells or mediated by some soluble factors produced by the stimulants. It was found that a significant amount of interferon (IFN) was produced by the SpA CoI-stimulation but not by the Protein A-stimulation, although the latter usually induced augmentation of NK-cell activity not less than SpA CoI-stimulation. IFN produced by SpA CoI was considered to belong to alpha-type IFN, because it was stable at pH 2.0 and could be neutralized effectively by anti-IFN alpha antibody. Kinetics of NK-cell activation by SpA CoI (but not by Protein A) were very similar to those by IFN alpha. Furthermore, augmentation of NK-cell activity with SpA CoI-stimulated supernatant was inhibited almost completely by diluted anti-IFN alpha antibody, whereas augmentation with Protein A-stimulated supernatant could not be abolished by the same treatment. It was, therefore, suggested that augmentation of NK-cell activity with SpA CoI might be ascribable in most part to the IFN induced, whereas Protein A can stimulated NK or T cells directly or soluble factors other than IFN might work as well.

Antibodies, Viral↗
PubMed1982

[Clinical diagnosis of well differentiated cervical adenocarcinoma with plentiful mucous secretion].

Six cases of cervical adenocarcinoma with plentiful mucous secretion were recently experienced. They were clearly distinguishable from the majority of adenocarcinoma without plentiful mucous secretion from the standpoint of clinical and colposcopic findings. As common features deduced from clinical and colposcopic findings, it could be pointed out that portio vaginalis become swollen as a whole showed resiliency as of a rubber ball, and that erosion widespread over nearly entire surface of portio vaginalis is accompanied with a plentiful mucus. On the surface of erosion was found neither localization of ulcer, nor necrosis and nor bleeding trend even in cases under progressing. Colposcopic findings were distinguished with features of papillea-, net-and transformation zone-like, and mixed type. On the other hand, all the cases were evaluated cytologically to be positive with difficulty. And they were histologically well differentiated adenocarcinoma with plentiful mucus. The distinctive feature in the clinical and colposcopic findings identified for the cases of well differentiated adenocarcinoma with a plentiful mucus was evaluated to be useful means to assist the diagnosis of cervical adenocarcinoma.

Adenocarcinoma, Mucinous↗
PubMed1982

[Evaluation of measuring serum trypsin by radioimmunoassay and clinical application for the study on the exocrine function in diabetics (author's transl)].

Measurement of serum concentration of trypsin by RIA-Gnost Trypsin kit (Hoechst-Japan) was evaluated. The clinical usefulness of measuring serum trypsin level in diabetic patients was assessed. The measurement of trypsin using the radioimmunoassay (RIA) kit revealed good precision and reproducibility with intraassay error ranging from 3.6 to 5.5% in C.V. corresponding to mean trypsin concentration of 236.5-838.7 ng/ml and interassay error ranging from 8.1 to 11.1%. Tests for recovery and dilution were satisfactory for clinical use. Clinical materials included 35 normal subjects, 88 diabetics, 22 patients with liver diseases, 3 with acute pancreatitis, 7 with chronic pancreatitis and 3 with chronic renal failure. Serum trypsin concentration in normal controls was 157.6 +/- 59.9 ng/ml (m + 1 S.D.). Diabetic patients treated with diet therapy alone revealed serum trypsin level of 203.6 +/- 74.8 ng/ml (n = 50). In diabetics treated with sulfonil urea serum trypsin was 171.3 +/- 83.0 ng/ml (n = 25). In patients receiving insulin serum trypsin level was 90.5 +/- 49.0 ng/ml (n = 13). In patients with liver diseases, acute pancreatitis, chronic pancreatitis and chronic renal failure serum trypsin concentration were 236.9 +/- 88.0, 520.1 +/- 80.0, 113.0 +/- 75.6, and 2557 +/- 2771 respectively. Our results may indicate impaired pancreatic exocrine function in patients with severe diabetes mellitus. Increased serum trypsin level in diabetics treated with diet therapy may be due to stimulated excretion of trypsin resulted from restricted food intake. However, further study in larger number of patients is needed.

Diabetes Mellitus↗
PubMed1982

Synthesis of carbon-bridged pyrimidine cyclonucleosides.

6,5'-Cyclo-5'-deoxyuridine 2',3'-cyclic phosphate and its derivatives were prepared as the conformationally fixed substrates of pancreatic ribonuclease. 6,6'-Cyclouridine, 6,3'-methylene-cyclo-2', 3'-dideoxyuridine, and 6,2'-ethylene-cyclo-2'-dexoyuridine were prepared as the new types of C-cyclopyrimidine nucleosides fixed in the anti conformation. The relationship between the sign and magnitude of CD Cotton bands and glycosylic torsion angles is discussed.

Animals↗
PubMed1981

Purification of human lymphoblastoid interferon by a simple procedure with high yields.

Human Namalwa cell interferon, induced by Sendai virus and composed of a single species with molecular weight of 17,000, was purified to 4.5 X 10(8) international reference units/mg of protein by a combination of salt precipitation, ion exchange chromatography, metal chelate chromatography, hydrophobic chromatography, and polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. By immunization of a rabbit with this purified interferon and by extensive absorption with Namalwa cells and an impurity column, highly specific antibody was obtained. Namalwa cells, treated with 5-bromo-2'-deoxyuridine, produced 10-fold more interferon upon induction by Sendai virus. Interferon in this case consisted of heterogeneous species with molecular weight ranging from 15,000 to 24,000. These heterogeneous interferon molecules were purified to 7.6 X 10(8) international reference units/mg of protein by successive chromatography using immobilized highly specific rabbit anti-interferon antibody, Blue Sepharose, and immobilized goat anti-rabbit IgG antibody. The overall recovery of interferon activity was 72%, and the purity of the final preparation was ascertained by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate.

Cell Line↗
PubMed1981

Tubular forms of hepatitis B surface antigen bind with the nucleus of 22-nm spherical HBsAg particles.

Spherical hepatitis B surface antigen particles (HbsAg) of 22-nm diameter were treated with sodium dodecylsulphate in the absence of reducing agents, and their nuclei were exposed. Factors that interact with the nucleus of HbsAg were detected in the serum of HBsAg carriers who were seropositive for hepatitis B e antigen and identified as tubular forms of HBsAg. The other categories of hepatitis B antigen, Dane particles, and 22-nm spherical HBsAg did not bind with the nucleus of HBsAg. When tubular forms of HBsAg had been treated with a proteolytic enzyme, they lost the reactivity to bind with the nucleus of HBsAg. On the basis of the results obtained, tubular forms of HBsAg bear the receptor of protein nature for the nucleus of 22-nm spherical HBsAg. The receptor allows rapid determination of tubular forms by a haemagglutination method for the evaluation of their clinical and epidemiological implications.

Carrier State↗
PubMed1981

Electrophysiological effects of lactates in mammalian ventricular tissues.

Effects of excessive lactates on electrophysiological parameters of Purkinje and ventricular muscle fibers, excised from dog hearts, were studied by the microelectrode technique. The application of 60mM-lactic acid produced the following changes in membrane potentials in both tissues: slight depolarization of resting potentials of 7 to 8 mV; decrease in the max.dV/dt of action potentials by an average of 10% from the control value; and shortening of action potential durations as well as of effective refractory periods by 17%. These effects appeared earlier in the Purkinje fibers than in the ventricular muscle fibers. The voltage dependence, max.dV/dt, was not changed after the lactate perfusion. This pointed to the depolarization of the resting potential as a cause of the decrease in the max.dV/dt of action potentials. In the Purkinje fibers, slow diastolic depolarization became manifest after the lactate application. Excessive lactates moderately depressed the slow response activity in both tissues. The perfusion of 60 mM-Na lactate had essentially similar effects in both tissues. In the ventricular muscle fibers, the voltage clamp experiments revealed that excessive lactate decreased the slow inward current and barely affected the steady-state K current. The results indicate that excessive lactates promote conditions likely to lead to a re-entry into the ventricle and, at the same time, to enhance the ectopic impulse formations in ventricular conducting tissues.

Action Potentials↗