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Biomedical subjects

T Sakurami

Publications and source records attributed to T Sakurami.

At least 55 records · Page 3Linked to original sources
PubMed1978

[In vitro stimulation of peripheral blood lymphocytes with allogeneic lymphocytes (mixed lymphocyte culture, MLC) or phytomitogens in patients with Hashimoto's thyroiditis (author's transl)].

To investigate the role of peripheral blood lymphocytes in thyroid auto-immunity, the in vitro responsiveness to allogeneic lymphocytes (MLC) or phytomitogens was examined in the lymphocytes from patients with Hashimoto's thyroiditis. The MLC responses of 16 patients were compared with those of age- and sex-matched normal controls. All possible combinations between lymphocytes from the patients and controls were mixed in both one-way and two-way MLC. From the results of one-way MLC, the stimulatory capacity and responding capacity of lymphocytes from the patients and controls were calculated respectively as follows: : formula: (see text) To the same allogeneic stimulation of Nm (mitomycin-treated stimulating lymphocytes from controls), lymphocytes from the patients (H) exhibited a lower response (P greater than 0.05) than did those from the controls (compare (1) with (3)). Phytomitogen-response was simultaneously studied for residual cells of MLC. Lymphocytes from the patients showed a significantly decreased (P less than 0.01) response to phytohemagglutinin-p and concanavalin A in comparison with the response of normal lymphocytes. From these results, it was suggested that cell-mediated immunity in vivo was impaired in Hishimoto's thyroiditis.

Adolescent↗
PubMed1978

[Subpopulations of peripheral lymphocytes in insulin-dependent diabetics (author's transl)].

There is an increasing evidence that autoimmune mechanisms may have a role in the pathogenesis in insulin-dependent diabetics. The numerical and functional study of peripheral blood lymphocytes in diabetes mellitus might indirectly contribute to the understanding of its pathogenesis. In this study, detection of peripheral blood T lymphocytes was measured by rosettes with sheep red blood cells (SRBC), and B lymphocytes were measured by immunofluorescence with specific antiserum to immunoglobulins. The mean (+/- SD) percentage of SRBC was 67.6 +/- 7.2 in 21 normal subjects, 71.5 +/- 7.0 in 15 insulin-dependent diabetics, and 68.6 +/- 6.7 in 30 insulin-independent diabetics. There was no difference in the absolute T-lymphocyte number per mm3 in these three groups. Insulin-dependent diabetics showed a normal percentage and absolute number of B lymphocytes when compared with normal subjects.

Adult↗
PubMed1977

[Leucocyte migration inhibition test in diabetes mellitus (author's transl)].

The leucocyte migration inhibition test (LMT) using the agarose plate method introduced by Clausen is simple and highly reproducible. Using human pancreas extract and beef insulin as antigen, LMT was performed on ten patients with insulin dependent diabetes, twenty patients with insulin independent diabetes, and twelve healthy controls. The migration index was expressed as a percentage of migration calculated from the following formula. Migration index (MI) = average areas of migration in test suspension/average areas of migration in control suspension. Using human pancreas extract, the mean migration index for the insulin dependent diabetics (87.6 +/- 11.1) was significantly lower than in the normal subjects (99.3 +/- 6.3) (p less than 0.05). Using beef insulin as antigen for the insulin dependent diabetics and insulin independent diabetics, the mean migration indices (+/- SD) were 95.8 +/- 14.9 and 98.7 +/- 12.3 respectively. The corresponding values for the control group were 98.9 +/- 7.8. Cellular hypersensitivity to human pancreas extract was shown in the leucocyte migration inhibition test with insulin dependent diabetics, but a negative result was obtained with beef insulin.

Adult↗
PubMed1977

[Leucocyte migration inhibition test in diabetes mellitus (author's transl)].

The leucocyte migration inhibition test (LMT) using the agarose plate method introduced by Clausen is simple and highly reproducible. Using thyroglobulin, mitochondria and thyroid microsomal fractions as antigens, LMT was performed on ten patients with insulin dependent diabetics, twenty patients with insulin independent diabetics, eleven patients with Graves' diseases, ten patients with chronic thyroiditis and ten healthy controls. The migration index was expressed as a percentage of migration and calculated from the following formula; (formula: see text). Using a thyroid microsomal fraction, the mean migration index for the insulin dependent diabetics (93.2 +/- 11.6) was significantly lower than in the normal subjects (104.3 +/- 10.9) (0.025 less than p less than 0.05). Using thyroglobulin as an antigen for the insulin dependent diabetics and insulin independent diabetics, the mean migration indices (+/-SD) were 93.7+/-10.8 and 102.8+/-10.6 respectively. The corresponding values for the control group were 100.6+/-7.3. Using a mitochondria, MI values for insulin dependent and independent diabetics were 97.7+/-5.5 and 99.6+/-12.6 respectively, while MI value for the control group was 103.9+/-13.6. The mean migration indices obtained with the mitochondria and thyroglobulin were not significantly depressed when the diabetic groups were compared with the normal subjects.

Cell Migration Inhibition↗
PubMed1976

[Rheumatoid factor in diabetes mellitus (author's transl)].

This study was undertaken to try to determine the incidence of rheumatoid factor in patients with diabetes mellitus by the Hyland RA slide test and the RAHA test (the sensitized sheep red cell agglutination test). Sera from 507 patients with diabetes mellitus and 2073 controls were investigated for the presence of rheumatiod factors. RA test was positive in 8.7 per cent and RAHA test in 6.1 per cent in sera from patients with diabetes mellitus compared to 4.1 percent and 3.6 per cent respectively in healthy subjects.

Adolescent↗
PubMed1976

[Thyroglobulin and microsomal antibodies in diabetes mellitus in childhood (author's transl)].

The purpose of our study was to determine the incidence of thyroid antibodies in diabetes mellitus in childhood and to discuss the correlation between thyroid antibodies and insulin antibodies. The sera of 50 children with diabetes mellitus and 437 children as disease controls were tested by thyroglobulin and microsome-coated tanned red cell hemagglutination test (Fuji Zoki Co. Tokyo). One of the 50 children with diabetes mellitus (2%) was positive with antithyroglobulin antibodies and eleven (22%) were positive with antimicrosomal antibodies compared to 0.4% and 1.1% respectively in 437 disease controls. To clarify the association of insulin antibodies and thyroid antibodies in diabetes mellitus in childhood, insulin antibodies were demonstrated by using a modified method described by Wright. One of the 33 children with negative insulin antibody (2.8%) was positive with antithyroglobulin antibodies and eight (24%) were positive antimicrosomal antibodies. No evident correlation was observed between antithyroid antibodies and insulin antibodies.

Adolescent↗
PubMed1975

[The clinical and immunological association of insulin antibodies and thyroid antibodies in diabetics (author's transl)].

We have already reported a high rate of occurrence of antimicrosomal antibodies in diabetes mellitus. Thirteen of 507 diabetics (2.5%) were positive with antithyroglobulin antibodies and thirty-one (6.1%) were positive with antimicrosomal antibodies compared to 2.3% and 2.5% respectively in normal controls. Two of 34 insulin dependent diabetics (5.9%) were positive with antithyroglobulin antibodies and ten (29.4%) were positive with antimicrosomal antibodies compared to 2.3% and 4.4% respectively in 473 insulin independent diabetics. To clarify the association of insulin antibodies and thyroid antibodies in diabetics, antithyroid antibodies in 507 diabetics were tested by tanned red cell hemagglutination test and insulin antibodies were demonstrated by using a method descrived by Wright in a modified form. Twelve of 482 diabetics negative-insulin antibody (2.5%) were positive with antithyroglobulin antibodies and thirty(6.2%)were positive with antimicrosomal antibodies. Only one of 25 positive-insulin antibody (4%) was positive with antithyroid antibodies respectively. No evident correlation was observed between antithyroid antibodies and insulin antibodies.

Adolescent↗
PubMed1975

[Incidence of thyroglobulin and microsomal antibodies in normal subjects (author's transl)].

In the course of studies on the occurrence of antithyroid antibodies in various thyroid disorders, serum antibodies to microsome of thyroid epithelial cells, as well as circulating antibodies to thyroglobulin, are demonstrated by tanned red cell hemaggulutination. These thyroglobulin and microsome-coated tanned red blood cells can be efficiently demonstrated with a commercially prepared reagent. (Fuji-Zoki Co.) The sera of 2,350 normal subjects were tested by these thryoid autoantibodies tests. Fifty-one (2.3 per cent) of the sera of 2,350 normal subjects showed a positive reaction for thyroglobulin antibodies, and fifty-nine (2.5 per cent) persons showed a positive reaction for microsomal antibodies. The incidence of thyroglobulin and microsomal antibodies in males and females were progressively greater with age, particularly between ages 60 to 69.

Adolescent↗