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Biomedical subjects

T Sakurami

Publications and source records attributed to T Sakurami.

At least 37 records · Page 2Linked to original sources
PubMed1981

HLA-DRW4 antigen linkage in patients with hypertrophic obstructive cardiomyopathy.

To determine the association of histocompatibility (HLA) genes in patients with hypertrophic cardiomyopathy, we determined HLA-A, HLA-B, HLA-C, and HLA-DR specificities in 33 Japanese patients (15 with the obstructive type off hypertrophic cardiomyopathy, and 18 with the nonobstructive type). HLA-DRW4 was found in 73% of patients with hypertrophic obstructive cardiomyopathy, as compared to 33% of 144 normal controls (p < 0.005). HLA-DRW4 occurred in 33% of those with hypertrophic nonobstructive cardiomyopathy, and there was no significant difference as compared with controls. Thus, hypertrophic obstructive cardiomyopathy is associated with genes in the HLA-DR region and immunogenetic factors linked to HLA appear to play a role in the pathogenesis. This work is the first attempt at demonstration of HLA-DR antigen in hypertrophic cardiomyopathy.

Adult↗
PubMed1981

Absence of HLA-DRW2 in Japanese pemphigus vulgaris.

17 Japanese patients with pemphigus vulgaris were examined by HLA typing. HLA-DRW2 was found in none of them as compared to 56 (39%) of 144 Japanese controls (p = 0.02). The association of HLA-DRW4 with pemphigus vulgaris was not recognized in our study. HLA-A, B and C typings were also performed, showing relatively high frequencies of the B15, BW35 and CW4. However, the occurrence of these specificities was not statistically significant. It is suggested that DRW2 plays an important role in the susceptibility to pemphigus vulgaris.

Adult↗
PubMed1981

Cytochemical demonstration of transferrin in the mitochondria of immature human erythroid cells.

The direct immunoperoxidase technique was employed to show the localization of transferrin in immature human erythroid cells. The present method is interesting in that use was made of a small marker (HRP-Fab' complex) and in that the endogenous peroxidase was blocked under a controlled condition- this ensured good structural preservation allowing electron-microscopic examination. Cytoplasmic transferrin was found not only in the micropinocytotic vesicles but also within the mitochondrial membrane. Similar findings were made in all the immature erythroid cells examined, each of which was in a different erythropoietic condition. Thus, it may be concluded that the present study provides morphological evidence for the endocytotic iron transport to the mitochondria.

Endocytosis↗
PubMed1980

[Thyroglobulin-binding and anti-thyroglobulin antibody-secreting lymphocytes in human peripheral blood (author's transl)].

Human peripheral blood lymphocytes were studied with the use of fluorescinated human thyroglobulin (FITC-Tg) for the presence of cells which bind human FITC-Tg (Tg-BL) and which secrete a thyroglobulin antibody (Tg-SL) by culturing them for 5 days with pokeweed mitogen. In seven out of fifteen (47%) patients with Hashimoto's thyroiditis, an average of 0.18% of lymphocytes were able to bind FITC-Tg, while only one out of six (17%) patients with Graves' disease was able to bind them. No Tg-BLs were detected in five patients with miscellaneous autoimmune diseases and five normal subjects. Tg-BLs were identified as B lymphocytes by their inability to make rosettes with sheep red blood cells. Tg-SLs were detected in six out of eight (75%) patients with Hashimoto's thyroiditis and two out of five (40%) patients with Graves' disease. The positive rates of Tg-BL and Tg-SL were fairly well correlated with the thyroglobulin antibody titers.

Antibodies↗
PubMed1980

Islet-cell antibodies and HLA type in Japanese insulin-dependent diabetics.

Pancreatic islet-cell antibodies (I.C.Ab.) were detected in the sera of 6 of 123 patients with insulin-dependent diabetes mellitus of recent onset, compared with only 2 positive results among the 434 control sera. The prevalence of humoral I.C.Ab. was strongly dependent on the duration of the diabetes, being 4 of 6 I.C.Ab. positive patients during the first year from diagnosis and falling to 2 of 6 I.C.Ab. positive patients at one to three years. Over four years from the time of diagnosis, there was no I.C.Ab. in the sera of the diabetics. Six I.C.Ab. positive in our group of 123 diabetic patients showed no association with any particular B locus antigen or DYT.

Adult↗
PubMed1979

[In vitro stimulation of peripheral blood lymphocytes with allogeneic lymphocytes or phytomitogens in patients with insulin-dependent diabetes mellitus (author's transl)].

The lymphocyte transformation response to the allogeneic lymphocytes (mixed lymphocyte culture, MLC) was determined in nineteen well-controlled insulin-dependent diabetics (IDD) and nineteen matched normal subjects. All possible combinations between lymphocytes from the patients and controls were mixed in both one-way and two-way MLC. From the results of one-way MLC, the stimulatory capacity (SC) and responding capacity (RC) of IDD lymphocytes were compared with those of normal lymphocytes as follows: (1) Nm leads to N: 10,538 +/- 3,937 N; normal lymphocytes (2) Nm leads to D: 8;466 +/- 5,387 D; IDD lymphocytes (3) Dm leads to N: 7,562 +/- 3,088 m; mitomycin-treated stimulating lymphocytes (4) Dm leads to D: 7,102 +/- 4,873 (leads to; stimulatory direction, results; M +/- SD cpm) IDD lymphocytes showed a marked depressive function as stimulators (SC, (1) -- (3)), but the RC of IDD lymphocytes was unchanged ((1) -- (2)). Phytomitogen-response was studied simultaneously for the same responding lymphocytes (N, D) of MLC. IDD lymphocytes exhibited significantly decreased responses to phytohemagglutinin P, pokeweed mitogen and concanavalin A.

Adult↗
PubMed1979

The effect of thoracic duct drainage on lymphocyte dynamics and clinical symptoms in patients with rheumatoid arthritis.

Thoracic duct drainage (TDD) was performed in 4 patients with severe rheumatoid arthritis. Clinical effects were apparent in all during drainage, but the term of TDD and the cumulative number of lymphocytes drained had no direct relation to the improvement of clinical symptoms. The number of lymphocytes in the peripheral blood increased despite discharge of lymphocytes from the thoracic duct in the very early stage of drainage, suggesting that lymph drainage from thoracic duct accelerates migration of lymphocytes from lymphocyte pools to the blood stream. Biopsy specimens of synovial membranes obtained post-TDD showed marked decrease of mononuclear cell infiltration as compared to the specimens obtained preoperatively. These findings suggest that clinical effectiveness may be due not only to systemic immunosuppression induced by lymphocyte depletion but also to accelerated migration of inflammatory cells from the synovial tissues to the blood stream occurring with dynamic change of lymph flow during TDD.

Adult↗
PubMed1979

HL-A and hypertrophic cardiomyopathy.

HL-A antigens were determined in 26 unrelated Japanese patients with hypertrophic cardiomyopathy. Several antigens were more common in patients compared with controls, but statistically significant differences were not evidenced. We also studied two families in which many had a hypertrophic cardiomyopathy. All the affected individuals revealed HL-A-A9 and B7, while none among the unaffected family members had HL-A-B7. Our findings suggest that the HLA-A system may play some role in the pathogenesis of hypertrophic cardiomyopathy with familial occurrence.

Adolescent↗
PubMed1979

HLA in hypertrophic cardiomyopathy and rheumatic heart disease.

1. HLA antigens were determined in 30 Japanese patients with hypertrophic cardiomyopathy. Several antigens were more common in patients compared with controls, but statistically significant differences were not evidenced. In families one and two, six of seven kindred who inherited HLA-A9 and B7 had the disease. None of five kindred lacking HLA-B7 showed evidence of the disease. In families three and four, affecting family members had HLA-A2 and BW-35. Our finding suggest that the HLA system may play some role in the pathogenesis of hypertrophic cardiomyopathy with familial occurrence. 2. Twenty patients with rheumatic valvular heart disease were also studied. There was no significant difference in frequencies of HLA antigens between patients and controls.

Adolescent↗
PubMed1979

Thyroglobulin and microsomal antibodies in patients with insulin dependent diabetes mellitus and their relatives.

The sera for 88 parents and 9 siblings of 73 patients with insulin dependent diabetes mellitus in childhood and 437 controls matched in age and sex, were tested by the thyroglobulin and microsome-coated tanned red cell hemagglutination test (Fuji-Zoki Co. Tokyo). None of 73 children with diabetes mellitus had antithyroglobulin antibodies, whereas twelve (16.4%) had antimicrosomal antibodies compared with the incidence of 0.4% and 1.1%, respectively, in 437 controls. In the parents and siblings of these probands, thyroid antibodies were also found in increased incidence. The incidence of antimicrosomal antibodies in the 68 mothers was significantly higher than in controls matched for age and sex, but the incidence of the positive thyroid antibodies in the 20 fathers and 9 siblings was not significantly different from that in control populations. The incidence of thyroid antibodies tended to be higher, though not significant, in parents and siblings of diabetic children with positive thyroid antibodies than in those of diabetics with negative ones. These findings suggest that immunogenetic factors may be responsible for the pathogenesis of some cases of diabetes mellitus in childhood.

Adolescent↗
PubMed1979

Circulating immune complexes in the serum of diabetes mellitus in childhood by a modified 125I-C1q binding test.

The 125I-C1q binding test for the detection of soluble immune complexes in native unheated human serum was applied to the study of sera from 52 patients with diabetes mellitus in childhood. This radiolabeled C1q binding test is more sensitive and reproducible among the various methods proposed for the detection of immune complexes. The 125I-C1q binding activity in 52 sera from diabetes mellitus in childhood was 9.47 +/- 0.36% compared to 6.94 +/- 0.74% in normal controls. 125I-C1q binding values in diabetes mellitus in childhood were significantly higher than normal controls. Slight high values were seen in 3 patients with positive anti-DNA-antibodies in diabetes mellitus in childhood. 125I-C1q binding was not significantly increased in patients with positive antithyroid antibodies and insulin antibodies. There was no significant correlation between the duration of diabetes and 125I-C1q binding activity.

Adolescent↗
PubMed1979

Antimicrosomal antibodies, gastric parietal cell antibodies and antinuclear factors in insulin dependent diabetes mellitus.

Thyroid antimicrosomal antibodies, gastric parietal cell antibodies (PCA) and antinuclear factors were studied in 208 insulin dependent diabetic (IDD) according to the duration of diabetes and patient's age at the time of testing. Antimicrosomal antibodies were found in 11 out of 47 (23.4%) IDD with the duration of less than one year, however this value declined to 13.1% at 1 to 3 years, 15.3% at 4 to 5 years, 10.8% at 6 to 10 years and 5.8% at more than 10 years. Of the 47 IDD, 7 (14.8%) were positive for gastric parietal cell antibodies. The prevalence of PCA declined with increasing duration of diabetes. However, this decrease in the prevalence of antimicrosomal antibodies and PCA was not so extreme as that of pancreatic islet cell antibodies. Antinuclear factors did not reveal a significant correlation with the duration of diabetes. In normal controls, the prevalence of antimicrosomal antibodies, PCA and the antinuclear factors increased progressively with age. In IDD, the prevalence of the antinuclear factors was also progressively greater with age. However, the prevalence of antimicrosomal antibodies in IDD decreased with age and those of PCA showed the lowest percent in the 40-69 year-age group.

Adolescent↗
PubMed1979

The leucocyte migration inhibition test and subpopulations of peripheral lymphocytes in insulin-dependent diabetics.

The leucocyte migration inhibition test (LMT) was performed by the agarose plate method with thyroid and pancreatic antigens in patients with insulin-dependent or independent diabetes mellitus. The mean migration indices with thyroglobulin, thyroid mitochondria and beef insulin were not significantly different in insulin-dependent diabetics from those in insulin-independent diabetics or normal controls. However, significant inhibition of leucocyte migration was observed in insulin-dependent diabetics when thyroid microsome or pancreatic extract was used as antigen. Although no significant difference was found in the percentages of T and B lymphocytes between insulin-dependent diabetics and insulin-independent diabetics or normal controls, the results of LMT strongly suggest the presence of cellular immunity against the thyroid and pancreas in insulin-dependent juvenile-onset diabetes.

Adult↗