Search PubMed⌕ Search

Biomedical subjects

T Otsuka

Publications and source records attributed to T Otsuka.

At least 379 records · Page 21Linked to original sources
PubMed1985

Structure of the chromosomal gene for granulocyte-macrophage colony stimulating factor: comparison of the mouse and human genes.

A cDNA clone that expresses granulocyte-macrophage colony stimulating factor (GM-CSF) activity in COS-7 cells has been isolated from a pcD library prepared from mRNA derived from concanavalin A-activated mouse helper T cell clones. Based on homology with the mouse GM-CSF cDNA sequence, the mouse GM-CSF gene was isolated. The human GM-CSF gene was also isolated based on homology with the human GM-CSF cDNA sequence. The nucleotide sequences determined for the genes and their flanking regions revealed that both the mouse and human GM-CSF genes are composed of three introns and four exons. The organization of the mouse and human GM-CSF genes are highly homologous and strong sequence homology between the two genes is found both in the coding and non-coding regions. A 'TATA'-like sequence was found 20-25 bp upstream from the transcription initiation site. In the 5'-flanking region, there is a highly homologous region extending 330 bp upstream of the putative TATA box. This sequence may play a role in regulation of expression of the GM-CSF gene. These structures are compared with those of different lymphokine genes and their regulatory regions.

Amino Acid Sequence↗
PubMed1985

Acute traumatic intraventricular hemorrhage in children.

Four cases are reported of acute traumatic intraventricular hemorrhage in children revealed by computed tomography (CT). In most cases, the intraventricular hemorrhage had been produced by severe sagittal impact. All of the children were 7 or less on the Glasgow Coma Scale (GCS) on admission. Three had hemorrhage in all ventricles, and one had an intraventricular hemorrhage in the lateral and fourth ventricles. One of them showed enhancement of the blood-filled ventricles, with the appearance of increased ventricular size, on the contrast enhancement CT. Two children with ventricular dilation died soon after injury, although ventricular drainage had been performed; one child remained in a persistent vegetative state, and the other enjoyed good recovery with conservative therapy. Certain features of acute traumatic intraventricular hemorrhage in children are discussed in relation to this series.

Brain Concussion↗
PubMed1985

Renin-stimulating test in children.

Plasma renin activity (PRA) was measured after various stimuli to evaluate a renin-stimulating test and to screen suppressed PRA in 60 children with labile hypertension aged 10 to 14 years. There was a significant difference between supine PRA and that after both standing (for 15 or 60 minutes) or frusemide administration (P less than 0.001, respectively); however, there was no statistical difference between the PRA while standing or after frusemide. Children who gripped a hand dynamometer for three minutes did not show any change in PRA. Standing upright for 15 minutes or supine rest for 90 minutes after the administration of frusemide i.v. are easily applicable to children and seem the most appropriate renin-stimulating test in childhood. No subjects showed suppressed PRA in this study.

Adolescent↗
PubMed1985

Isolation of cDNA for a human granulocyte-macrophage colony-stimulating factor by functional expression in mammalian cells.

A cDNA sequence coding for a human granulocyte-macrophage colony-stimulating factor has been isolated from cDNA libraries prepared from mRNA derived from concanavalin A-activated human T-cell clones. The libraries constructed in the pcD vector system were screened by transfecting COS-7 monkey cells with DNA pools to express the products encoded by full-length cDNA inserts. By assaying the cell supernatants, we identified clones encoding a factor that stimulates the formation of granulocyte and macrophage colonies from human progenitor cells. These results demonstrate that identification of full-length cDNAs for many colony-stimulating factors may be achieved entirely on the basis of detecting the functional polypeptide produced in mammalian cells.

Amino Acid Sequence↗
PubMed1985

Electrolyte excretion in 12-hour urine and in spot urine. Relationship to plasma renin activity and aldosterone concentration in older children.

Plasma renin activity (PRA) and urinary electrolyte excretion were measured in 137 healthy children aged 6 to 14 years. Plasma aldosterone concentration (PAC) was measured in 52 of the children. Nocturnal 12-hour urine was collected in 110 of the children. Spot urine was collected on two occasions, once just before lying down, once after 90-min supine rest in another 27. Na/K ratio and fractional Na excretion rate (FENa) in 12-hour urine showed a significant inverse correlation with PRA or PAC. Na excretion (mmol/min, mmol/mmol creatinine), Na/K ratio and FENa in the spot urine following 90 min in a supine position showed a significant inverse correlation with PRA or PAC, but they failed to show a significant relationship to PRA or PAC in the spot urine preceding supine rest. A spot urine after 90 min in the supine position is collected easily and hence most appropriate to study the relationship between Na excretion and PRA or PAC clinically.

Adolescent↗
PubMed1985

B cell activity and regulatory T cell function in systemic lupus erythematosus by human B cell colony formation.

We have examined the ability of B cells from patients with systemic lupus erythematosus (SLE) to form colonies in vitro. Significantly more B cell colonies growing in the absence of irradiated T cells were observed in patients with active SLE. Helper T cell function of active SLE was normal. Culture supernatants of SLE T cells had significantly less activity to support the B cell colony formation. Our results suggest that the hyperactivity of B cells in SLE may reside in the lupus B cells themselves and that SLE T cells are defective in releasing the soluble factor.

Adolescent↗
PubMed1985

[Anterior pituitary hormone in severe head injury--with special reference to the GH, TSH, LH and FSH response to TRH and LH-RH].

Measurement of the serum level of anterior pituitary hormones (GH, TSH, LH and FSH) and the response of these hormones to their releasing hormones were made in 45 uncomplicated head injury patients within 24 hours after injury. We classified these 45 patients into three groups according to the Glasgow Outcome Scale of six months after head injury, such as good outcome group (GR or MD), poor outcome group (SD or PVS) and dead group. The serum level of the anterior pituitary hormones in dead group were higher than those in the good or poor outcome group, especially the level of GH. The serum level of GH was 14.7 +/- 3.0 ng/ml in the dead group, whereas 5.8 +/- 0.9 ng/ml in the good outcome group and 4.0 +/- 2.1 ng/ml in the poor outcome group. The response of TSH, LH or FSH to the administration of TRH or LH-RH were depressed in the dead group. On the other hand, there were normal response of TSH, LH or FSH following administration of TRH or LH-RH in the other two survival groups. These findings suggested that the measurement of the anterior pituitary hormones and the response of these hormones to their releasing hormones were useful to evaluate the severity of head injury.

Adolescent↗
PubMed1985

Hepatitis B.

Since the recognition and description of the hepatitis B virus in the early 1970s, the spectrum of disease caused by this virus has become clearly defined. Our understanding of the hepatitis B virus has rapidly increased over the past two decades due to the discovery of various serologic markers and correlation of these with clinical and epidemiological data. Advances in techniques in molecular biology, such as recombinant DNA and hybridization have also recently added impetus to hepatitis research. The word-wide consequences of acute and chronic infection have prompted intense research into the development of effective, but as yet expensive, forms of prophylaxis.

Acute Disease↗