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Biomedical subjects

T Ota

Publications and source records attributed to T Ota.

At least 307 records · Page 17Linked to original sources
PubMed1981

Chemical and biological properties of a peptidoglycan isolated from Treponema pallidum kazan.

A peptidoglycan layer of Treponema pallidum kazan was isolated by solubilization of whole cells with 1% warm sodium dodecyl sulfate and subsequent digestion of an insoluble residue with proteases. Electron microscopy revealed that the peptidoglycan was isolated as a single-layered sacculus of less than 5 nm in thickness, freed from axial filaments and an envelope sheath. An isolated peptidoglycan fraction was mainly composed of glucosamine, muramic acid, alanine, glutamic acid, ornithine, and glycine in molar ratios of 0.65:0.68:1.63:1.00:0.75:1.03. Amino (N)- and carboxyl (C)-terminal amino acid analyses suggested the involvement of at least a part of the glycine residue in cross-linking between the amino group of ornithine residue at one strand of the stem peptide subunit and the carboxyl group of alanine of the neighboring strand. The treponemal peptidoglycan lacked the immunoadjuvant activity both to stimulate antibody production and to induce delayed-type hypersensitivity against ovalbumin, as well as the properties necessary to stimulate guinea pig and mouse splenocytes and guinea pigs peritoneal macrophages, unlike the cell walls or peptidoglycans (group A type of Schleifer and Kandler's classification, Bacteriol. Rev. 36:407-477, 1972) isolated from many bacterial species parasitic to the mammal. However, the peptidoglycan activated the human complement system through the alternative pathway, as well as the classical one, and caused a liberation of 5-hydroxytryptamine in rabbit blood platelets in a similar manner to the cell wall peptidoglycans of both group A and B types.

Amino Acids↗
PubMed1981

Changes in somatostatin release from perfused pancreas of streptozotocin-induced diabetic rats.

To clarify the mechanism of an increased response of diabetic D-cells to arginine, we studied the changes in responsiveness of pancreatic D-cell to this agent with the passage of time after the onset of diabetes and the effect of chronic insulin substitution on the altered D-cell function utilizing the perfused pancreas of streptozotocin diabetic rats. The magnitude of somatostatin (SRIF) response to arginine (10 mM) was progressively increased with the duration of diabetes. The integrated response of SRIF during a 20 min period of an arginine infusion one week after the injection of streptozotocin was not different from that in the normals, but it was increased by 240% at 8 weeks and by 390% at 15 weeks. The exaggerated pancreatic SRIF release observed in 8 week diabetic rats was partially ameliorated with the daily insulin replacement (15.0 +/- 1.0 units/kg of B.W.). This was accompanied by a partial recovery from growth failure. The integrated amount of SRIF secreted during an arginine-stimulated period (20 min) was inversely correlated with an increase in body weight during an 8 week period (r = -0.741, p less than 0.001). From these results, it might be concluded that the alteration in diabetic D-cell function may be related to the metabolic and hormonal abnormalities of diabetes, rather than insulin deficiency itself.

Animals↗
PubMed1979

Syntheses and antiinflammatory actions of 4,5,6,7-tetrahydroindazole-5-carboxylic acids.

A novel series of 1-aryl-4,5,6,7-tetrahydro-1H-indazole-5-carboxylic acids and 2-aryl-4,5,6,7-tetrahydro-2H-indazole-5-carboxylic acids were synthesized via condensation between a phenylhydrazine and a 2-(hydroxymethylene)cyclohexanone-4-carboxylate, and the antiinflammatory activity was determined. In the carrageenan edema test, 1-aryl-4,5,6,7-tetrahydro-1H-indazole-5-carboxylic acids exhibited fairly high antiinflammatory activity. However, the 2-aryl isomers were far less active than the former. The most active compound of the series was 1-phenyl-4,5,6,7-tetrahydro-1H-indazole-5-carboxylic acid, which had an ED50 value of 3.5 mg/kg.

Analgesics↗
PubMed1978

On psychiatric symptoms of neuro-Behçet's syndrome.

Studies were made on the groups of psychiatric symptoms of Neuro-Behçet's syndrome in six cases considered to be brain stem encephalitis, including one case of autopsy. Psychiatric symptoms appeared from one to eight years after neurological symptoms first occurred. This group of psychiatric symptoms can be divided roughly into the early-stage, middle-stage, terminal-stage and episodic groups. The initias-stage symptom group concerns mainly a change in, and regression of, personality, consisting of being childish, shallow, indifferent, careless, rude, slow in action, euphoric, or depressive. The middle-stage symptom group is made up of indifference, forced smiling, forced crying, amnesia, recent memory impairment, and disorder of sleep rhythm. The terminal-stage symptom group comprises the "lock-in" syndrome, which if advanced, will become Apallisches Syndrome. The episodic symptom group is made up of pathologically abnormal experiences such as hallucinations and delusions and symptoms due to a decline in the level of consciousness such as confusion, delirium and twilight state. Dementia hitherto mentioned in Neuro-Behçet's syndrome should be classified as subcortical dementia.

Adult↗