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Biomedical subjects

T Osada

Publications and source records attributed to T Osada.

At least 199 records · Page 11Linked to original sources
PubMed1987

Endocytotic internalization of alpha-2-macroglobulin: alpha-galactosidase conjugate by cultured fibroblasts derived from Fabry hemizygote.

Endocytotic internalization of alpha-galactosidase by cultured fibroblasts derived from a patient with Fabry's disease was achieved via receptor-mediated endocytosis of alpha-2-macroglobulin (alpha-2-M). alpha-galactosidase of coffee beans was conjugated to alpha-2-M when the latter was treated with trypsin. Internalization of the conjugate resulted in an increase of alpha-galactosidase activity in the crude cell extracts. The observed internalization was blocked by the presence of bacitracin, an inhibitor of binding between alpha-2-M and its receptor on the cell surface. When the cells were incubated at 4 degrees C with the conjugate, internalization was also inhibited. The alpha-galactosidase activity in the cells was saturated when the concentration of the conjugate in the medium was 40 micrograms/ml. Since non-conjugated alpha-galactosidase was not effectively internalized, the observed internalization of the conjugate was mediated by recognition of alpha-2-M by its receptor. The effective internalization of alpha-galactosidase described in this paper has a potential use in the enzyme replacement therapy of Fabry's disease.

Cells, Cultured↗
PubMed1987

[The Su-polysaccharide skin test in lung cancer].

Skin testing with intradermally injected Su-Polysaccharide (extracted from Su strain Streptococcus bacteria) was performed in 41 cases of lung cancer. Su-polysaccharide skin test results were correlated, to some extent, with the patient's age, clinical stage and performance status and showed a similar trend to the simultaneously performed PPD skin test. These results suggested the potential usefulness of Su-Polysaccharide skin test results as one of the parameters of immunological status of patients with lung cancer. It was also demonstrated that skin reaction to Su-Polysaccharide was increased specifically after OK-432 immunotherapy and was well correlated with the prognosis of the disease. The Su-Polysaccharide skin test was thus considered to be a useful parameter for monitoring the immune response to OK-432 immunotherapy in lung cancer and also one of the parameters of prognostic value.

Adult↗
PubMed1987

[A case of blind-ending bifid ureter].

A case of blind-ending bifid ureter in a 6-year-old girl is presented. Excretory urography revealed a right blind-ending ureter which was resected surgically. Besides this, she had megaureters on both sides, and uretero-vesiconeostomy was undertaken. Although urinary tract infection had continued for several months, she had no episode of fever after operation.

Child↗
PubMed1987

[Acute renal failure after renal trauma in a case of a functional solitary kidney].

Herein we report a case of acute renal failure after renal trauma in a patient with contralateral hypoplastic kidney. The patient was treated conservatively including temporary hemodialysis successfully. In the case of renal trauma, the status and function of the contralateral kidney must be evaluated first, and in the case of a solitary kidney, treatment should be centered on the preservation of its function.

Abdominal Injuries↗
PubMed1986

Unusual properties of crocodilian ovomacroglobulin shown in its methylamine treatment and sulfhydryl titration.

The inhibitory activity of chicken and crocodilian ovomacroglobulins against trypsin was measured before and after their incubation with methylamine. The result for crocodilian ovomacroglobulin showed that methylamine treatment destroyed half of its activity, in unique contrast to human alpha 2-macroglobulin and chicken ovomacroglobulin for which methylamine either destroys the inhibitory activity of the former completely or does not affect that of the latter at all. Free sulfhydryl groups of chicken and crocodilian ovomacroglobulins were titrated with 5,5'-dithiobis(2-nitrobenzoic acid) before and after incubation with trypsin. Prior to the incubation with trypsin the chicken and crocodilian proteins respectively had 0 and 1 titratable sulfhydryl per molecule of Mr 720,000. After treatment with trypsin the crocodilian protein had 3.5-4 titratable sulfhydryls, whereas there were no titratable sulfhydryls in the chicken protein. After denaturation of the crocodilian protein in sodium dodecyl sulfate at 100 degrees C the number of titratable sulfhydryls was 4. Chicken ovomacroglobulin again did not have an appreciable number of titratable sulfhydryls under similar denaturing conditions. Incubation of crocodilian protein with [14C]methylamine showed an incorporation of at least 2 mol of methylamine per molecule. The result indicated the presence of three intramolecular thiol ester bonds in crocodilian ovomacroglobulin with differential stability against external perturbations.

Alligators and Crocodiles↗
PubMed1985

Structural changes in alpha-2- and ovomacroglobulins studied by gel chromatography and electron microscopy.

The structural change that occurs in alpha-2-macroglobulin upon its interaction with methylamine or chymotrypsin was studied by high-performance gel chromatography and electron microscopy. The result enabled us to estimate the Stokes radius of the protein as 8.8 nm and 7.9 nm before and after binding with the proteinase, respectively. The methylamine-treated protein also had the Stokes radius of 7.9 nm. Similar studies on the chicken and crocodilian ovomacroglobulins showed that these homologues of alpha 2-macroglobulin had Stokes radii of 9.2-9.3 nm and 8.5-8.7 nm before and after binding with chymotrypsin. Their Stokes radii did not change as a result of the methylamine treatment. Electron micrographs of the native and altered forms of the three proteins are presented. This study introduces a simple and quantitative method to study the structural change of alpha 2-macroglobulin and its homologues.

Alligators and Crocodiles↗