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Biomedical subjects

T Ono

Publications and source records attributed to T Ono.

At least 1,153 records · Page 64Linked to original sources
PubMed1985

Systemic malacoplakia in a breeding pig.

An adult breeding pig had multiple disseminated lesions in several organs of the thoracic and abdominal cavities. Histopathologically, the lesions consisted of accumulated histiocytic cells with a few giant cells and a mild infiltration of lymphocytes and plasma cells associated with concentric laminated spherical Michaelis-Gutmann bodies which stained strongly with haematoxylin and varied in diameter. The histological features of the lesions were similar to those of malacoplakia reported in man. The present case seems to be unique because the characteristic lesions were found in many organs including kidneys, liver, spleen, lungs, gall bladder, small intestine and several lymph nodes. The possibility of the haematogenous spread of malacoplakia was suggested by the frequent formation of the lesions in perivascular areas. The present case should be described as "systemic malacoplakia in a breeding pig".

Animals↗
PubMed1985

Induction and modification of erythropoiesis in a suspension culture system derived from long-term bone marrow culture of Syrian hamster.

Cells in a subculture system derived from long-term hamster bone marrow culture were induced to differentiate into hemoglobin-producing cells in response to added erythropoietin. Treatment of the subculture with dimethyl sulfoxide or other 'Friend inducers' but not 5-azacytidine effected no remarkable induction, though they enhanced the effect of externally added erythropoietin. 3-Aminobenzamide, a weak inducer of Friend cell differentiation [Morioka, K., K. Tanaka, M. Ishizawa and T. Ono: Dev. Growth Differ. 24, 507-512 (1982)], had a strong inhibitory effect on erythropoiesis in the subculture system. Phorbol esters and glucocorticoids (both are known to be strong inhibitors of Friend cell differentiation) acted as strong inhibitors of the differentiation in our system as well. The present system was shown to provide a useful in vitro model to compare the differentiation of normal erythroid progenitors with the previously established systems of neoplastic cells.

Animals↗
PubMed1985

Absence of gross change in primary DNA sequence during aging process of mice.

The possibility of age-associated changes in DNA sequence in terms of amplification and rearrangement was examined in mouse spleen, liver and brain using the method of Southern transfer and filter hybridization. The DNA regions studied were at and around nine cloned sequences, most of which are known to move or amplify in certain situations. No detectable age-associated change, however, was observed in all DNA regions studied. These results suggest that widespread DNA sequence rearrangements or amplifications do not occur during the ageing process in mice.

Aging↗
PubMed1985

Movement and non-movement related pallidal unit activity during bar press feeding behavior in the monkey.

Activity was recorded from 358 neurons in the globus pallidus (GP) of monkeys (Macaca fuscata) during an operant feeding task consisting of 3 stages: (1) food or non-food presentation (1st stage); (2) bar pressing (2nd stage); and (3) food acquisition and ingestion (3rd stage). There were two kinds of neurons, one with high and the other with very low (almost silent), spontaneous firing rates. Two hundred and four neurons (57%) responded in one or more of the feeding stages. Of the 21 neurons which responded in the 1st stage, two responded selectively to food presentation, and 19 responded to both food and non-food visual presentation. One hundred and seventy-four neurons (49%) and 107 neurons (30%) responded in the 2nd and 3rd stages, respectively, and 106 (30%) of these were directly related to specific feeding motor acts such as arm extension, flexion, bar pressing, grasping, chewing etc. Both high and low firing neurons responded to motor acts with sharp or gradual onset. More than half of those that responded to arm extension showed laterality (contra or ipsi)- and function (extension or flexion)-dependent responses. The incidence of the motor related neurons was higher in the caudodorsal part of the GP. On the other hand, about one third, especially in the rostroventral part of the GP, showed dissociating responses in that they responded during bar pressing for food or during ingestion in an operant task, but not during bar pressing for non-food or during forcible ingestion. The magnitude of firing changes during arm extension and bar pressing depended on the nature of the food. Moreover, in trials using new food or false (model) food, firing changes during bar press appeared or disappeared within a few trials with no correlation to bar press movement. These data suggest heterogeneous functions within the GP; the caudodorsal part is strictly concerned with motor execution and preparation, while the rostroventral part is not related to motor function directly, but may rather be important in coupling internal, motivational information to the motor system.

Animals↗
PubMed1985

Topographic organization of projections from the amygdala to the hypothalamus of the rat.

Afferent fibers from the amygdala to subdivisions of lateral, ventromedial and dorsomedial hypothalamic nuclei were investigated in rat by retrograde transport of horseradish peroxidase. Small (intranuclear size) peroxidase deposits were placed in hypothalamic nuclei by iontophoresis of a tracer solution containing poly-L-alpha-ornithine which greatly limited diffusion. The medial, central and amygdalo-hippocampal nuclei of the amygdala were found to be the major donors of amygdaloid afferent fibers to the hypothalamus, but there was also substantial labeling of somata in cortical, basomedial, basolateral and lateral amygdaloid nuclei and the intra-amygdaloid bed nucleus of the stria terminalis. No fibers projected from the posterior cortical nucleus of the amygdala to the hypothalamus. Most amygdaloid projections to the lateral hypothalamic area originated in the anterior half of the amygdala, while projections to the ventromedial hypothalamic nucleus arose along the entire length of the amygdala except the posterior cortical nucleus. The amygdalo-hippocampal area projects to the medial hypothalamus. Other amygdaloid nuclei project to both the medial and lateral hypothalamic nuclei. These topographic organizations of amygdaloid afferent fibers to various subdivisions of the hypothalamic nuclei are discussed and compared with other anatomical studies on these connections.

Amygdala↗
PubMed1985

Immunological study of the rat hypothalamic ventromedial nucleus.

Specific anti-sera were prepared by injecting the homogenates of rat ventromedial nucleus (VMH) and caudate nucleus (Cd) into the rabbit. Anti-VMH serum, after absorption of common components in rat normal serum and anti-Cd serum, reacted specifically to the rat VMH with only one precipitation line. Anti-VMH serum was successfully applied to 80 VMH neurons by electrophoresis through 5 barreled micropipettes. Fresh anti-VMH serum caused an irreversible response (increase and subsequent sudden cessation of firing) in 8 of the 25 VMH neurons tested. Anti-VMH serum reversibly inhibited 32 of 80 VMH neurons and 13 of these were also tested with glucose. Discharge rates of 12 of the 13 neurons increased by glucose. Most of these neurons were not affected by anti-Cd serum or normal rabbit serum. Results of these immunological and electrophysiological studies suggest the existence of specific membrane receptor binding sites on the glucoreceptor neurons in the VMH. These sites afford one route for producing the excitatory effect that glucose has on VMH neurons.

Animals↗
PubMed1985

Synthesis and antibacterial activity of 2,2'-dithiobis(benzamide) derivatives against Mycobacterium species.

A series of compounds, which are analogues of 2,2'-dithiobis(benzamide), were synthesized and tested for in vitro antibacterial activity against Mycobacterium tuberculosis H37Rv including resistant strains against streptomycin, kanamycin, or isonicotinic acid hydrazide. MICs of these compounds against atypical mycobacteria, Mycobacterium kansasii and Mycobacterium intracellulare were also examined. Structure-activity relationships were found in a series of (acyloxy)alkyl ester derivatives depending upon the length of alkyl carbon chain. The MIC of the most potent compound, 2,2'-dithiobis[N-[3-(decanoyloxy)propyl]benzamide] [56] was superior or at least equivalent to streptomycin, kanamycin, and ethanbutol. All the compounds showed no cross-resistance between the current antitubercular agents.

Animals↗
PubMed1985

A case report of malignant lymphoma with Hashimoto's thyroiditis.

The authors studied a case of malignant lymphoma arising in a patient with Hashimoto's thyroiditis. Histologically, a malignant, large cell, immunoblastic lymphoma was revealed, with the surrounding area showing changes typical of Hashimoto's thyroiditis. Immunoperoxidase staining proved the lymphoma cells to have intracytoplasmic immunoglobulins (IgA, k). Infiltrating interfollicular plasma cells containing the same kind of intracytoplasmic immunoglobulins (IgA, k) were present in the surrounding Hashimoto's thyroiditis lesions. Thus, it is possible that the interfollicular infiltrating plasma cells evolved into a malignant lymphoma in this case of Hashimoto's thyroiditis.

Aged↗
PubMed1985

Human histidine-rich glycoprotein: simultaneous purification with antithrombin III and characterization of its gross structure.

The simple and simultaneous purification of histidine-rich glycoprotein (HRG) and antithrombin III (AT III) from human plasma and gross structural characterization of HRG have been performed. The purification method consists of two chromatographic procedures using heparin-agarose and DEAE-Sephadex. The yields of HRG and AT III were 22 mg and 70 mg, respectively, from 1 liter of plasma. The purified HRG is a single-chain polypeptide with a molecular weight (Mr) of 75,000 on sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, indicating it was the native form of this protein. Cyanogen bromide cleavage of HRG, followed by analysis of the amino acid composition and determination of the amino-terminal sequence of each purified cyanogen bromide fragment established that the gross structure of HRG consisted of three cyanogen bromide fragments; an amino-terminal CN-50 kDa fragment (Mr 50,000) and a carboxy-terminal small fragment of eight amino acids, and a CN-30 kDa fragment (Mr 30,000) between them. As to the amino acid composition of the CN-30 kDa fragment, it had an unusually high content of histidine (25 mol%), suggesting the presence of a histidine-rich region(s) in the carboxy-terminal half of the molecule. These results together with our previous results (Koide, T., Odani, S., & Ono, T. (1982) FEBS Lett. 141, 222-224) and those of Morgan (Morgan, W.T. (1985) Biochemistry 24, 1496-1501) imply that HRG is composed of at least two domains with distinct functional properties; i.e. an amino-terminal domain with heparin-binding ability and a carboxy-terminal domain with heme- and divalent metal-binding abilities.

Amino Acids↗
PubMed1985

Establishment of an efficient purification method and further characterization of 32K calmodulin-binding protein in testis.

A heat-stable 32K calmodulin-binding protein has been purified approximately 3,670-fold from porcine testis to apparent homogeneity as judged by both sodium dodecyl sulfate polyacrylamide gel electrophoresis and polyacrylamide gel electrophoresis under native conditions. The purification employed calmodulin-Sepharose 4B affinity chromatography; elution was performed with a free Ca2+ gradient. This provided a simple and efficient procedure, and approximately 1.62 mg of pure heat-stable calmodulin-binding protein was obtained from 390 g of porcine testis with a yield of 47% in activity. The purified protein was asymmetric (f/fo = 1.89) and consisted of a single polypeptide of Mr = 32,000. It is a highly acidic protein (pI = 3.9) with a diffusion coefficient of 5.4 X 10(-7) cm2/s, a sedimentation coefficient of 1.43 S, and a Stokes radius of 39.5 A in its free form and 41.3 A in its complex form with calmodulin. The extent of inhibition of phosphodiesterase by the calmodulin-binding protein was affected by the order of addition of the agents to the reaction mixture. The extent of inhibition was maximal when phosphodiesterase was added last, while it was minimal when the calmodulin-binding protein was added last. This protein was indistinguishable from a heat-stable calmodulin-binding protein in rat testis (Ono, T., Koide, Y., Arai, Y., & Yamashita, K. (1984) J. Biol. Chem. 259, 9011-9016).

3',5'-Cyclic-AMP Phosphodiesterases↗
PubMed1985

Immunohistochemical analysis of antimelanoma monoclonal antibodies, with special reference to fetal tissue distribution.

An immunohistochemical study using 2 antihuman melanoma monoclonal antibodies designated as MoAb 225.28S and MoAb 653.40S was carried out on various human skin tumors, including malignant melanoma as well as on normal and fetal tissues by indirect immunofluorescence technique. Specific immunofluorescence was observed not only in malignant melanoma cells but also in cells of pigmented nevi, basal cell epithelioma, normal hair follicles, and some fetal tissues. Both monoclonal antibodies were revealed to be able to recognize the common antigenic determinant shared by several skin tumors, including malignant melanoma, and fetal tissues. Therefore, both monoclonal antibodies might recognize premature antigen of both melanocytic and keratinocytic cell lineage.

Antibodies, Monoclonal↗
PubMed1985

Dermal melanocytes and elastic fibers.

The relationship between dermal melanocytes and elastic fibers was studied by electron microscopy. In 3 patients with dermal melanocytosis, the long cytoplasmic processes of the melanocytes were aligned along the long axis of the elastic fiber, and encircled or embraced these fibers. The same intimacy existed between melanocytes and elastic fibers in the skin of cynomolgus macaques, who normally have dermal melanocytes. These findings suggest that dermal melanocytes, for reasons yet to be determined, interact with elastic fibers under these special conditions.

Aged↗
PubMed1985

Dysdifferentiative nature of aging: age-dependent expression of MuLV and globin genes in thymus, liver and brain in the AKR mouse strain.

The amount and sequence complexity of RNA transcribed by the murine leukemia virus (MuLV) genome and to the alpha- and beta-globin genes in thymus, brain and liver were measured throughout the life span of AKR mice using a cDNA X RNA hybridization technique. RNA complementary to the complete sequence of specific cDNA probes for both MuLV and globin was found in nuclei and cytoplasm of thymus, brain and liver at all ages studied. No significant age-dependent change in the amount or sequence complexity of globin RNA was detected. The amount of MuLV RNA in both nuclei and cytoplasm of thymus increased about five times from 2 to 5 months of age, but no significant change was observed over this age range in MuLV RNA from liver and brain. By the age of 10 months, most of the mice had developed leukemia and the thymus showed a further increase in the amount of MuLV RNA. Nuclear RNA from liver and brain then showed a significant increase in the amount of MuLV, but no change in the amount of MuLV was found in the cytoplasm. No qualitative age-dependent changes in the MuLV RNA sequence complexity in thymus, liver and brain were detected. These results suggest that in the AKR mouse strain for the three tissues studied, an age-dependent relaxation occurs in the repression of the MuLV genome but not for alpha- and beta-globin genes. The rate of the depression of MuLV genes in the short-lived AKR mouse strain appears similar to that of the long-lived C57BL/6J mouse strain. This age-dependent relaxation of MuLV genes appears to be limited to the nuclei. Thus, the presence of a specific regulatory system for the transport of MuLV RNA through the nuclear membrane which does not deteriorate with age is indicated.

Aging↗
PubMed1985

Pulmonary aspergillosis in apparently healthy young rabbits.

Pulmonary aspergillosis was discovered in apparently healthy young rabbits. Thirty-five rabbits of various ages raised in the same rabbitry were examined by histological, ultrastructural and microbiological methods. The pulmonary lesions were found only in rabbits younger than five weeks old. These lesions contained characteristically shaped hyphae surrounded by eosinophilic radiating projections--"asteroid bodies." The significance of the disease condition and the structure of the "asteroid bodies" is discussed.

Animals↗
PubMed1985

Ovulation induction in clomiphene-resistant anovulatory women: differential follicular response to purified urinary follicle-stimulating hormone (FSH) versus purified urinary FSH and luteinizing hormone.

We studied 15 anovulatory women undergoing ovulation induction with purified human urinary FSH or purified human urinary FSH and LH [human menopausal gonadotropins (hMG)]. All patients had either sporadic or no vaginal bleeding after progesterone therapy and failed to ovulate after receiving clomiphene (250 mg for 5 days) plus hCG. Other causes of infertility were ruled out. Sixteen cycles of FSH and 12 cycles of hMG were administered according to a standard protocol. Estradiol, progesterone, androstenedione, testosterone, LH, and FSH concentrations were quantitated by RIA. Follicular diameter was determined using ultrasound. There was no significant difference in the amount of FSH or hMG used per patient, in the duration of therapy before hCG administration, or in the length of the luteal phase in any patient. There was a difference in the number of follicles greater than 1000 mm3 per cycle in those patients receiving FSH compared to the number in those receiving hMG [2.8 +/- 1.3 (+/- SEM) vs. 4.4 +/- 1.5 follicles; P = 0.026). The maximum follicular phase serum estradiol (18.3 vs. 34.8 ng/ml) and maximum luteal phase progesterone concentrations (1289 vs. 2808 pg/ml; P = 0.026) were also different between the FSH and hMG groups. Linear regression analysis revealed a significant correlation between the peripheral serum estradiol levels and the total follicular volume of follicles in the hMG-treated group which was not apparent in the FSH-treated group. These findings suggest that exogenous LH may not be required to induce folliculogenesis in anovulatory patients.

Adult↗