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Biomedical subjects

T Ono

Publications and source records attributed to T Ono.

At least 1,063 records · Page 59Linked to original sources
PubMed1987

[Early pregnancy diagnosis by the ultrasonographical device and observation of fatal growth in cynomolgus monkeys (Macaca fascicularis)].

Usefulness of the ultrasonographical device (USD) for early diagnosis of pregnancy and for observation of fetal growth was examined in the cynomolgus monkey (Macaca fascicularis). Firstly, 50 females, each of which was mated for 24 hours in an individual cage, were used. They were diagnosed for pregnancy by the use of USD 15 to 29 days and 35 days after mating. Then, 20 of them were judged to be pregnant. The early pregnancy diagnosis was based on the detection of gestational sac (GS) in the uterus. The GS was detected 20.0 +/- 1.8 days after mating. The duration from the day on which GS was first detected to the day on which the heart beat of embryo (HBE) was detected averaged 5.6 +/- 1.5 days. In 17 of these 20 pregnancies, GS was detected 17 to 23 days after mating. As regards the remaining three pregnant cases, GS and HBE were simultaneously detected on the 28th day of pregnancy in two cases, and on the 35th day in one case. Secondly, 225 females who were mated by the routine 3 day-mating system were used. Pregnancy diagnosis by USD was conducted on the 21st, 28th and 35th day after mating. Ninety-six of the 225 females were judged to be pregnant. In 33 (34.1%) of the 96 pregnant cases, GS was detected on the 21st day of pregnancy. In 57 females (59.4%), GS was detected on the 35th day. Furthermore, it was able to judge conditions of fetal growth by measuring the size of GS and biparietal diameter.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1987

Introduction of new indices into continuous avoidance test--a trial to detect the effects of sodium chloride and mannitol on avoidance behavior.

A personal computer was introduced in conditioned lever-press avoidance experiment in Sidman situation. Experimental data were analyzed with both classic indices such as response rate and shock rate and new indices introduced in this experiment. New indices include sum of squares of shock-shock intervals, mean of shock-response time and number of shock-response time less then 5 sec. Results obtained in diazepam-treated mice were consistent with those reported previously by the classic method. In mannitol-treated mice, significant changes of the avoidance behavior could be detected by both the classic index and our new indices. By the new indices other than classic indices, the effect of administration with sodium chloride on the avoidance behavior could be detected, whereas the classic indices could not detect the effect. Though the meanings of these indices i physiological and toxicological aspects should be appreciated in future study, the experimental system reported in this paper might be applicable in the toxicological research to detect the effect of administration with various substances on the avoidance behavior.

Animals↗
PubMed1987

Pleiotropic effects of quercetin on the transformation of BALB 3T3 cells.

The effect of quercetin, a flavonoid, on the in vitro transformation of BALB 3T3 cells was examined. Quercetin at a low concentration (5 micrograms/ml) markedly suppressed the promoting action of 12-O-tetradecanoyl phorbol-13-acetate (TPA, 10 ng/ml) in this transformation system using 20-methylcholanthrene (MCA, 1 microgram/ml) as the initiator. At a concentration higher than 5 micrograms/ml, however, quercetin enhanced transformation by MCA. Quercetin itself was found to induce direct transformation weakly, but definitely, at a slightly higher concentration than that for enhancing MCA-induced transformation. These results indicate that quercetin has pleiotropic effects on the transformation of BALB 3T3 cells.

Animals↗
PubMed1987

Quantitative determination of cyclosporin A in whole blood and plasma by high performance liquid chromatography.

A simple, specific and sensitive method for monitoring therapeutic levels of the immunosuppressive agent cyclosporin A (CyA) in whole blood and plasma has been developed which uses a high performance liquid chromatography and involves a simple liquid-liquid extraction procedure. Samples were delipolysed with 0.1 mM dextran sulfate sodium salt, extracted with n-hexane, and chromatographed on a Lichrosorb Si-60 column with UV detection at 215 nm. Each of CyA and internal standard (cyclosporin D) is resoluted clearly as a single peak and no interfering peaks are observed on the chromatograms. The detection limit is 5 ng/ml in whole blood and plasma. The reproducibility assessed by repeated analysis is satisfactory, that is to say, each coefficient of variation of the within-run and between-run precision is below 4%. The analytical recovery of added CyA is almost complete. This method is sensitive enough for monitoring whole blood and plasma levels of CyA in renal transplant patients in the therapeutic dose range, and is also applicable to the study on the distribution of CyA between plasma and non-plasma fractions of blood in patients with renal allografts.

Adolescent↗
PubMed1987

[Clinical evaluation of primary lung cancer induced by exposure to asbestos].

Upon autopsy, asbestos was found in the lungs of 7 primary lung cancer patients out of 70. These seven-patients were men over 60 years old. All of them had occupational histories of exposure to asbestos. In histological classification, 4 of the cases were adenocarcinoma and the others were squamous. Six of them were in the peripheral area. According to chest X-rays two of the patients showed lung fibrosis (asbestosis), and all of them showed pleural plaque. Thus, we concluded that ten percent of the primary lung cancer cases autopsied in our hospital might be induced by exposure to asbestos.

Adenocarcinoma↗