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Biomedical subjects

T Ohno

Publications and source records attributed to T Ohno.

At least 757 records · Page 42Linked to original sources
PubMed1986

[Clinical trial of bestrabucil (KM 2210) on hematopoietic malignancies].

Twenty-one patients were entered for a clinical study of KM 2210, the benzoate of an estradiol-chlorambucil conjugate, in the treatment of hematopoietic malignancies. These included 4 cases of chronic lymphocytic leukemia (CLL), 5 cases of chronic myelogenous leukemia (CML), 5 cases of malignant lymphoma (ML) and 7 cases of multiple myeloma (MM). Twelve cases had prior chemotherapy. KM 2210 was given orally at a dose of 50-300 mg daily. Of 19 evaluable cases, two cases with CLL achieved complete response and 6 cases including 2 with CLL, 2 with CML and 3 with ML achieved partial response. There were no responders among the cases of MM. The partial and complete response rate was 47%. Toxicity included mild breast or nipple pain (28.6%), genital bleeding (9.5%), appetite loss (9.5%) and gynecomastia (4.8%). These side effects may have been due to increased levels of estrogen. Hematopoietic toxicity was mild and well tolerated. No cardiac, hepatic or renal toxicity was observed in this study. These results suggest that KM 2210 might be an effective candidate for the treatment of hematopoietic malignancies, especially CLL.

Aged↗
PubMed1986

[Treatment of malignant lymphoma under consideration of its biological behavior].

Malignant lymphoma is very heterogeneous in terms of its biological behavior. A strategy for the treatment of malignant lymphoma fundamentally requires a multidisciplinary field of treatment including mainly chemotherapy and radiotherapy in collaboration with surgical procedure and immunotherapy. The expressions of biological behavior such as histologic type, immunologic type, and stage of disease which influence the therapeutic effect and prognosis are actually very important in deciding a plan of treatment. Recent advances in chemotherapy have made it possible to cure advanced lymphoma patients, especially those with Hodgkin's disease. In this article the present state of treatment for malignant lymphoma is briefly reviewed from the viewpoint of its biological behavior.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1986

[Clinical features and treatment of Hodgkin's disease].

Hodgkin's disease is characterized by its peculiar histopathology and by the early and consistent existence of impaired cell-mediated immunity, although its oncogenetic mechanisms still remains unsolved. Recent progress in multidisciplinary treatment including mainly radiotherapy and chemotherapy has a possible the cure of advanced Hodgkin's disease. In this article the main aspects of clinical behavior and therapy in Hodgkin's disease were reviewed briefly in the light of some considerations of our experiences.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1985

Molecular basis of plant viral virulence; the complete nucleotide sequence of an attenuated strain of tobacco mosaic virus.

The total genome sequence of L11A, an attenuated strain of tobacco mosaic virus (TMV), has been determined. This strain is able to multiply in tomato plants without inducing any remarkable symptoms, but to protect them from later infection with virulent TMV strains. When compared with the recently published total genome sequence of TMV L (the virulent ancestral strain of L11A) ten base substitutions were found in the L11A genome. Seven of these occurred in the third letters of in-phase codons and did not influence amino acids. Only three, which were in the common reading frame for both the 130K and 180K proteins, resulted in amino acid changes. Together with the result of the partial sequence of RNA of L11, an intermediate strain in sequential isolation from L to L11A, it is observed that one base at the nucleotide position 1117 is changed from L to L11 and two bases at the positions 2349 and 2754 are changed from L11 to L A11.

Amino Acid Sequence↗
PubMed1985

Adrenomyeloneuropathy: report of a family and electron microscopical findings in peripheral nerve.

A family with adrenomyeloneuropathy (AMN) is reported, whose female carriers showed severe neurological and biochemical abnormalities. Cytoplasmic lamellar inclusions were found in endoneurial cells of the biopsied sural nerve of a male patient. In spite of the peripheral nervous system involvement in patients with AMN, there have been few reports of these inclusions in the peripheral nerves of such cases.

Adrenal Gland Diseases↗
PubMed1985

New method for bioavailability assessment of slow-release preparations of theophylline.

Variability in an individual's clearance of theophylline is an important consideration when estimating bioavailability. A method is described for compensating for this problem, using the serum concentration of theophylline and urinary excretion data on its major metabolites to make an estimation of the clearance after oral administration using the intravenous dose as reference. The method is particularly useful for assessing the bioavailability of slow-release theophylline preparations.

Administration, Oral↗
PubMed1985

Factors controlling the appearance of the immunofluorescent nuclear dots revealed with monoclonal antibody against microtubule-associated protein-1.

A monoclonal antibody raised against microtubule-associated protein-1 (MAP-1) binds to nuclei of normal human fibroblasts, its binding site being detected as intranuclear immunofluorescent dots. The percentage of cells showing these dots increased with insulin, hydrocortisone, epidermal growth factor (EGF), and transferrin in serum-free medium, their effects decreasing in this order. The appearance of dots induced by serum or insulin plus hydrocortisone was dependent on the Ca2+ concentration of the medium. The Ca2+ ionophore A23187 increased the rate of appearance of cells containing dots. Conversely, the calmodulin inhibitor W-7 blocked their appearance and decreased the number of cells containing dots. The dose-response curves of W-7 for inhibition of the appearance of dots and of DNA synthesis were essentially identical. These results suggest that the appearance of intranuclear MAP-1 antigen is controlled mainly by insulin, Ca2+ and calmodulin and is associated with DNA replication.

Antibodies, Monoclonal↗
PubMed1985

Ultrastructural study of long spacing collagen fibres and basal lamina in malignant schwannoma.

It was found by electron microscopy that extracellular darkly stained materials (DSM) observed abundantly in a case of malignant schwannoma were closely related to both basal lamina and fibrous long spacing collagen (FLS). The FLS were characterized by the cross bands with a 95 nm periodicity, and longitudinally aligned filaments, 9 nm in diameter, while DSM consisted of amorphous material, and 9 nm filaments. The filaments in DSM and FLS were similar in diameter and morphology to reticular fibres in basal laminae. The DSM were continuous with both dark bands of FLS and basal laminae. These results indicate that basal laminae may be the common origin of DSM and FLS. Ultrastructural features of longitudinal, transverse and oblique sections were described.

Aged↗
PubMed1985

Effects of 16,16-dimethyl prostaglandin E2 on ethanol-induced and aspirin-induced gastric damage in the rat. Scanning electron microscopic study.

Ethanol and aspirin were administered orally to fasted rats and the effects of 16,16-dimethyl prostaglandin E2 (given orally as a pretreatment) were studied using scanning electron microscopy. Gastric secretory studies (pylorus ligation for 2 h) showed that 3 and 30 micrograms/kg of 16,16-dimethyl prostaglandin E2 were nonantisecretory doses and that 100 micrograms/kg was an antisecretory dose. 16,16-Dimethyl prostaglandin E2, given orally at 3-100 micrograms/Kg, induced no appreciable changes in the gastric surface epithelial cells. Oral administration of 1 ml of 50% ethanol invariably induced, within 10 min, extensive exfoliation of surface epithelial cells throughout the corpus and antrum and exposed the lamina propria. 16,16-Dimethyl prostaglandin E2, given orally at 3, 30, and 100 micrograms/kg 30 min before ethanol treatment, had no protective effect. Aspirin, given orally at 30 or 100 mg/kg, also damaged the surface epithelium of both the corpus and the antrum within 10 min. This damage ranged from apical cellular erosions to widespread exposure of the lamina propria. 16,16-Dimethyl prostaglandin E2, given orally at 3 or 30 micrograms/kg 30 min before aspirin treatment, significantly inhibited the gastric damage induced by both 30 and 100 mg/kg of aspirin. The inhibition of damage index was about 50%-60% at either 30 or 100 micrograms/kg of 16,16-dimethyl prostaglandin E2. The mechanism of the protection seen with 16,16-dimethyl prostaglandin E2 remains to be determined.

16,16-Dimethylprostaglandin E2↗
PubMed1985

Microfilament-undercoated plasmalemmal infoldings in a human clear-cell sarcoma.

Unusual structures often found in the cytoplasm of tumor cells in a clear-cell sarcoma appeared as multilayered, concentric, oval, spiral, parallel arrays of cisternae in various planes of section. It was demonstrated that the cisternal membrane and cavity were continuous with plasmalemmas of tumor cells and the extracellular space, respectively, suggesting that the structures were formed by the intracytoplasmic infoldings of plasmalemmas. Another characteristic found in the structures was orderly microfilaments with an average diameter of 6.5 nm which were placed between the confronting plasmalemmas in the infoldings. The filaments which underlay the infolded plasmalemmas ran parallel to each other along the cytoplasmic surfaces of plasmalemmas approximately 15 nm apart. The regularly arranged filaments were found in the infolded plasmalemmas, but not beneath any other area of plasmalemmas. The short ends of long filaments appeared to bend toward the inner surfaces of plasmalemma and to be directly connected with the surface proper. These results show that the filaments may be closely associated with the plasmalemmal infoldings and included as the same category of plasmalemmal undercoat. Additionally, the biological significance of the structures is discussed.

Actin Cytoskeleton↗
PubMed1985

Demonstration of T antigens on the surface of cells transformed with primate polyoma viruses.

Primate polyoma virus-transformed hamster, mouse, and rat cell lines were examined by indirect immunofluorescence staining for cell surface-associated T antigens, by using a rabbit antiserum prepared against sodium dodecyl sulfate-denatured large T antigen of simian virus 40 (anti-SV40-SDS-T serum). Positive surface staining was shown not only on SV40-transformed cells, but also on BK and JC virus-transformed cells. In contrast, normal cells and cells transformed with mouse polyoma-, human adeno-, and murine sarcoma viruses were negative. The data on SV40-transformed cells confirmed the reports of others demonstrating the cell surface location of SV40 large T antigen, and the data on BK and JC virus-transformed cells proved that these cells have cell-surface T antigens that cross-react with anti-SV40-SDS-T serum.

Animals↗