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Biomedical subjects

T Ogino

Publications and source records attributed to T Ogino.

At least 397 records · Page 22Linked to original sources
PubMed1981

Transverse deficiency of the hand in one of monozygotic twins.

In this paper, we report on one male infant of twins with a left hand anomaly. The hand deformities were diagnosed as so called "congenital amputation" which is a synonym of "transverse deficiency" in Swanson's classification. The left hand showed spontaneous amputation at the level of the palm. There were two rudimentary digits on the stump, and an extremely hypoplastic thumb and little finger. The other twin had no anomaly. The results of laboratory findings on blood groups and HLA types suggest that these twins are monozygotic. From these facts, we considered that environmental factors are more important than genetic ones in the pathogenesis of transverse deficiency and symbrachydactyly of the hand.

Blood Grouping and Crossmatching↗
PubMed1981

Biochemical transformation of mouse cells by varicella-zoster virus.

Mouse L cells lacking the enzyme thymidine kinase (Ltk-) were infected with varicella-zoster virus (VZV). Even though virus did not replicate in Ltk- cells, the presence of virus antigen could be observed by use of an anti-complement immunofluorescent technique at 4 h post-infection and the VZV-specific thymidine kinase could be detected in VZV-infected Ltk- cells. Ltk-cells were converted to a tk+ phenotype (Ltk+) by infection with cell-associated VZV. Clones possessing the ability to grow in selective medium were isolated and cultured successfully for more than 20 passages. One of the clones grew very slowly, but other clones showed almost the same growth rate as that of the parental Ltk- cells. The chromosome analyses of Ltk- cells and transformed cells revealed that the isolated clones were of mouse origin. VZV-specific antigen could be detected in the nuclei of Ltk+ cell clones by an immunofluorescent test, while tk activity was greatly enhanced in extracts prepared from transformed cells and its activity was neutralized by hyperimmune serum against VZV.

Animals↗
PubMed1981

Familial tricho-rhino-phalangeal syndrome Type II.

Two cases, a father and daughter, with all the principal signs of tricho-rhino-phalangeal syndrome Type II are described, although nine previously reported cases were all sporadic. It is suggested that these patients have a genetic disorder with an autosomal dominant mode of inheritance. It may be reasonable to assume that a patient with relatively mild mental retardation, such as the father in the present report, could marry and have off-spring. Generalized aminoaciduria was found in the affected daughter.

Abnormalities, Multiple↗
PubMed1981

Specificities of human and rat brain enzymes of cholesterol ester metabolism toward very long chain fatty acids: implication for biochemical pathogenesis of adrenoleukodystrophy.

Specificities of the cholesterol-esterifying enzyme and the three cholesterol esterases in rat brain with respect to the chain length of fatty acids were examined. For each of the hydrolases, activities toward cholesteryl lignocerate and cerotate were generally less than 1% of that toward cholesteryl oleate. However, both lignoceric and cerotic acids were esterified at rates approximately 10% of that for oleic acid. In postmortem human control and adrenoleukodystrophy brains, the esterifying activity toward cerotic acid was on the average 25% of that toward oleic acid. The abnormal accumulation of cholesterol esters with very long chain fatty acids observed in adrenoleukodystrophy can therefore occur in the absence of deficient activities of the cholesterol esterases, if the free fatty acid pool of the brain contains an abnormal amount of very long chain fatty acids.

Acyltransferases↗
PubMed1981

In vitro estimation of cell kinetic parameters in human urinary bladder cancers.

The DNA synthetic time (T4) and the labeling index (LI) or urinary bladder carcinomas from 10 patients were measured by using the 3H-thymidine labeling technique in vitro under hyperbaric oxygen, and the potential doubling time (Tpot) was determined. The LI and Tpot were enormously variable depending on the histologic type, and ranged from 3.7 to 18.0% and from 48 to 136 hr, respectively. In contrast, Ts values fell in a rather narrow range. On comparing these results with the histologic patterns it was found that papillary carcinoma had longer Tpot than nonpapillary carcinoma and histologic grades were not well correlated with the LI's However, patients with higher LI and/or shorter Tpot showed poor prognosis. The practical significance of these kinetic parameters is discussed

Aerobiosis↗
PubMed1980

Biochemical study of adrenoleukodystrophy (ALD).

Adrenoleukodystrophy (ALD) is an x-linked hereditary neurological disorder characterized by the accumulation of cholesterol ester with long chain fatty acids in the brain and adrenal gland. We examined cholesterol ester metabolism for the postmortem brain tissues of ALD patients, using cholesterol ester with short and long chain fatty acids as the substrate for hydrolyzing enzyme, as well as short and long chain fatty acids for synthesizing enzyme. No enzyme abnormality was found. However, there was a discrepancy between hydrolytic and synthetic activities with short or long chain fatty acids. The findings suggest that the accumulation of cholesterol ester with long chain fatty acids in ALD brain is not due to enzyme abnormalities, but is a secondary phenomenon which comes from abnormal fatty acid metabolism causing a high concentration of long chain fatty acids.

Adrenal Gland Diseases↗
PubMed1980

Virus replication and localization of varicella-zoster virus antigens in human embryonic fibroblast cells infected with cell-free virus.

When human embryonic fibroblast cells were infected with cell-free varicella-zoster virus, virus replication began between 8 and 14 h postinfection, and 4 more h werp required for the virus to infect neighboring cells. Virus-specific antigens were traced by the anticomplement immunofluorescent antibody technique. Virus antigen was first detectable 2 h postinfection in the cytoplasma, and diffuse fluorescence was observed in the nucleus as early as 4 h after infection. The nuclear fluorescence got brighter and cytoplasmic fluorescence was observed at 14 h postinfection. The spread of virus to the neighboring cells was recognized in 18 h postinfection. In the period of 24 to 48 h, antigens were seen at the nuclear membrane region and in the cytoplasma. Very strong fluorescence was restricted mainly to the nucleus, when phosphonoacetic acid or cytosine arabinoside was added to the infected cultures and the cells were incubated for 48 h.

Antigens, Viral↗