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Biomedical subjects

T Oda

Publications and source records attributed to T Oda.

At least 757 records · Page 42Linked to original sources
PubMed1981

Immunoperoxidase study of alkaline phosphatase in testicular tumor.

Indirect immunoperoxidase staining was carried out on human testicular tumors using monospecific antibodies against placental (Regan) and intestinal isoenzymes of alkaline phosphatase (ALPase). The very high incidence of seminoma (approximately 90%) revealed positive staining of placental ALPase mainly on the cell membrane of tumor cells, whereas none of the seminoma showed presence of intestinal isoenzyme. Placental isoenzyme was not recognized in any embryonal carcinoma and interstitial cell tumor. The epithelial cells of the glandular elements of teratoma occasionally exhibited strong staining for intestinal ALPase and weak staining for placental ALPase. The appearance of Regan isoenzyme in seminomas might be considered possible conversion of hepatic to placental isoenzyme, a consequence of malignant transformation of spermatogenic cells. Regan isoenzyme appears to be a new tumor marker for seminoma and the frequent identification of Regan isoenzyme in seminoma may disclose a unique biologic characteristic of this germinal tumor.

Adult↗
PubMed1981

Determination of adriamycin (doxorubicin) and related fluorescent compounds in rat lymph and gall by high-performance liquid chromatography.

The concentrations of adriamycin (ADM) and related fluorescent compounds in lymph and gall were determined by high-performance liquid chromatography (HPLC) after a single intravenous injection into AH 109A tumour-bearing rats. HPLC was carried out by using Zorbax Sil as the stationary phase and chloroform--isopropanol--acetic acid--water--sodium acetate buffer (pH 4.5) (100:100:14:14:1) as the mobile phase, with a fluorescence spectrophotometric detector at an excitation wavelength of 470 nm and an emission wavelength of 585 nm. The detection limit for ADM was down to 1.0 ng/ml. In the thoracic duct lymph, the concentration of total ADM equivalent values (total ADM values) was maximal 30 min after injection and, after a subsequent decrease, increased gradually from 60 to 180 min. The ratio of total ADM in lymph to that in plasma at 180 min was 1.5 times that at 30 min. In gall, the total ADM showed a maximal level of 20.0 microgram/ml at 30 min.

Animals↗
PubMed1981

Gonadotropin and alkaline phosphatase producing occult gastric carcinoma with widespread metastasis of generalized bone.

A case is reported of a small primary occult gastric adenocarcinoma occurring in a 57-year-old man revealing widespread metastasis to generalized bone, and associated with high serum level of circulating human chorionic gonadotropin (hCG), carcinoembryonic antigen (CEA), and alkaline phosphatase (ALPase). The primary site of metastatic bone tumor was not clear until autopsy. Microscopic examination of the gastric primary revealed moderately differentiated tubular adenocarcinoma admixed with numerous signet-ring carcinoma cells without conspicuous trophoblastic differentiation. The elaboration of hCG and intestinal and placental isoenzyme of ALPase by carcinoma cells themselves was confirmed by the indirect immunoperoxidase method. The present case may be interesting because of unusual metastasis and simultaneous production of hCG, CEA, and intestinal and placental ALPase isoenzymes.

Adenocarcinoma↗
PubMed1981

Necessity of detergent for efficient puromycin-mediated release of nascent peptides from rat liver ribosomes.

Puromycin-mediated in vitro release of nascent peptides from rat liver ribosomes was significantly stimulated by the presence of low concentrations of a detergent, and the stimulation was much more marked with bound ribosomes than with free ribosomes. The release of nascent peptides from ribosomes could be carried out in two steps, first with puromycin in the absence of a detergent and then with a detergent, to give two separate nascent peptide fractions S1 and S2, respectively. Although S1 and S2 fractions were not significantly different in hydrophobicity and in the size of the puromycin-conjugated peptides when examined by alkyl-Sepharose column chromatography and SDS-polyacrylamide gel electrophoresis, the fractionation of the released peptides by immunoprecipitation showed significant difference in the distribution of the nascent peptides of two specific proteins, serum albumin and NADPH-cytochrome c reductase, between these two fractions. The nascent peptides of serum albumin were found mainly in fraction S1 obtained from bound ribosomes. On the other hand, a larger portion of the nascent peptides of NADPH-cytochrome c reductase was detected in fraction S2 from free ribosomes than in other fractions. The presence of a detergent is indispensable for efficient in vitro release of nascent peptides from ribosomes by puromycin and this finding may be important in studying the synthesis of specific proteins in mammalian cells.

Animals↗
PubMed1981

Involvement of free ribosomes in the early stage of secretory protein biosynthesis in rat liver.

Nascent peptides on free and bound ribosomes prepared from rat liver were labeled and released with [3H]puromycin, and the amounts of the nascent peptides of two secretory proteins, serum albumin and transferrin, were determined by immunoprecipitation with specific antibodies. An appreciable amount of serum albumin nascent peptides was associated with free ribosomes, although most of them were carried by bound ribosomes. Serum albumin nascent peptides associated with free ribosomes were smaller in size than those carried by bound ribosomes. When the products of cell-free translation programmed by free and bound polysomes were immunoprecipitated with antibodies, the percentages of serum albumin to the total translation products were in good agreement with those obtained in the nascent peptides experiment. Cell-free translation of free polysomes produced serum albumin with a similar molecular weight to the authentic one. Although nascent peptides of transferrin were detected only on bound ribosomes, and not on free ribosomes, the transferrin antibody immunoprecipitated 0.2% of total translation products of free polysomes. These observations indicate that free polysomes have complete messenger RNA's and shorter growing nascent peptides of serum albumin and transferrin.

Animals↗
PubMed1981

Clinical appraisal of the antideoxyribonuclease-B (ADNB) by means of streptonase-B test (Wampole).

Titers of antideoxyribonuclease-B (ADNB, by Streptonase-B test), antistreptolysin-O (ASO, by Rantz-Randall's micromethod) and Kinase test (antivaridase agglutination test) were performed on 118 sera of patients with suspected streptococcal infection and compared. Between ASO and ADNB, a fairly significant correlation was seen (r = 0.69), but many cases in which ADNB exceeded ASO were observed. Between Kinase and ADNB, no significant correlation was seen (r = 0.49). Successive determination of antibody titers were made in 14 consecutive patients with acute glomerulonephritis, anaphylactoid purpura nephritis, rheumatic fever and chronic rheumatic heart disease. In general, ADNB titer was significantly higher than ASO with few exceptions. The duration of high titer of ADNB was much longer than ASO Kinase titers were sometimes unstable. Throughout this study it has been noted that the result of ADNB was very easily to read and was highly reproducible. There were no nonspecific or pseudopositive reactions. Therefore, ADNB is useful for the diagnosis of streptococcal infection and its complication especially when other antibody titers give negative or doubtful results.

Adolescent↗
PubMed1981

Tissue distribution and antitumor effect of liposome-entrapped doxorubicin (adriamycin) in Ehrlich solid tumor-bearing mouse.

The usefulness of liposomes (in neutral, positively and negatively charged forms) as a carrier for adriamycin (ADM) was studied by examining the distribution of ADM and related fluorescent compounds in Ehrlich solid tumor-bearing mice. The mice were given free or liposome-entrapped ADM intraperitoneally. The distribution of ADM and related fluorescent compounds between the administration of the free form and liposome-entrapped form was measured by high performance liquid chromatography : The distribution was dependent on the form of the liposomes. The amounts of ADM and its metabolites in the mouse serum 20 min after administration of neutral-liposome-entrapped ADM were 10 times those after the administration of free ADM, 6 times those after the administration of a negatively charged form, and 3.5 times those in the administration of positively charged form. There was no marked difference in the concentrations of these compounds 5 h after administration. The concentration of these compounds in the liver 60 min after administration of each liposome-entrapped form of ADM were in inverse correlation with the concentrations in the serum obtained at 20 min after administration. Total concentrations of ADM and its metabolites in the tumors 20 min after administration of each entrapped form of ADM were 4-5 times that in administration of free ADM after 20 min. There were no marked differences in the concentration of ADM for administration of the various liposome forms. Statistically significant decreases in mean tumor weight were seen in the groups given neutral, positively and negatively charged liposome-entrapped forms compared to corresponding control groups given with free ADM.

Animals↗