Search PubMed⌕ Search

Biomedical subjects

T Nishimaki

Publications and source records attributed to T Nishimaki.

At least 163 records · Page 9Linked to original sources
PubMed1983

[Factors affecting the growth and extension of gastric cancer--analysis of retrospective observation].

It is our aim to clarify an importance of severe lymphoid infiltration and peptic ulcer to the growth and deep extension of the gastric carcinoma, using 38 cases of gastric carcinoma observed retrospectively from one year to nine years and six months. Four cases of gastric carcinoma with lymphoid stroma were found in this series. Its mucosal extension is markedly suppressed compared with that of carcinomas without lymphoid stroma. Thirteen cases clinically showed a distinct deep invasion into the gastric wall during the retrospective observation period of one year to nine years and six months, while 21 cases repeated peptic ulcers in the tumor tissue without a distinct deep invasion during the period of one year and one month to six years and three months. On an examination of the resected stomach, the former had a common histological characteristic, that is, no complication of peptic ulcer or, even if presents, relatively small peptic ulcers to the tumor mass in the submucosa or in the further deep layers. On the contrary, the latter always showed multiple or recurrent, relatively large ulcers to the tumor mass in the submucosa or in the further deep layers. It is concluded that the severe lymphoid infiltration and peptic ulcers in the gastric carcinoma are factors inhibiting the growth of gastric carcinomas.

Female↗
PubMed1982

Studies on the metabolism of unsaturated fatty acids. VIII. Induction of 2,4-dienoyl-CoA reductase in Escherichia coli on the addition of unsaturated fatty acids.

2,4-Dienoyl-CoA reductase has been detected in crude extracts of E. coli. The reductase was shown to be induced many fold when the cells were grown in the presence of linoleic or oleic acid. The activity profile of the reductase on gel filtration was different from those of other enoyl reductases. These results suggest that there are two pathways even in E. coli for the degradation of cis-4-decenoyl-CoA, which is an intermediate in the beta-oxidation of linoleic acid, as recently proposed in rat liver.

Chemical Phenomena↗
PubMed1982

Studies on the metabolism of unsaturated fatty acids. IX. Stereochemical studies of the reaction catalyzed by trans-2-enoyl-coenzyme A reductase of Escherichia coli.

The steric course of the reaction catalyzed by NADPH-dependent trans-2-enoyl-CoA reductase from Escherichia coli was investigated. trans-2-[6,7,8-2H7]Octenoyl-CoA was synthesized as a substrate and incubated with partially purified trans-2-enoyl-CoA reductase in the presence of 4R- or 4S-[4-2H1]NADPH. The deuterium-labeled octenoyl-CoA was also incubated with the reductase in the presence of NADPH in 2H2O. Aliquots of octanoic acids formed were analyzed, after esterification, by gas chromatography-mass spectrometry (GC-MS) to demonstrate that the pro-4R hydrogen of NADPH was incorporated into the C-3 position of octenoyl-CoA and that hydrogen from water was introduced into the C-2 position of octenoyl-CoA. The remaining portions of octanoic acids isolated from the incubation mixtures were converted to their CoA esters by the action of acyl-CoA synthetase, and they were dehydrogenated by treatment with acyl-CoA oxidase, which had previously been shown to catalyze the anti-elimination of the pro-2R and pro-3R hydrogen atoms of acyl-CoA. The deuterium contents of the products were also analyzed by GC-MS, and the results indicated that the reduction catalyzed by NADPH-dependent trans-2-enoyl-CoA reductase occurred by an anti-addition of hydrogen via a 2-Re, 3-Re attack on the trans-double bond of the substrate.

Deuterium↗
PubMed1982

Studies on the metabolism of unsaturated fatty acids. X. Purification and some properties of 2,4-dienoyl-CoA reductase from Escherichia coli.

2,4-Dienoyl-CoA reductase has been separated from Escherichia coli grown in the presence of linoleic acid and purified to homogeneity. The enzyme has a molecular weight close to 50,000 as determined by gel filtration on Sephacryl S-200 Super-fine. The reductase was rather stable in a buffer containing citric acid and kept its full activity on heating at 55 degrees C for 10 min in the pH range of 5.5 to 6.5, but was completely inactivated on heating at 58 degrees C for 10 min. Phosphocellulose column chromatography revealed that the reductase was not involved in the multi-enzyme complex (molecular weight of 260,000) of fatty acid oxidation.

Escherichia coli↗
PubMed1982

Studies on the metabolism of unsaturated fatty acids. XI. Alterations in the activities of enoyl-CoA hydratase, 3-hydroxyacyl-CoA epimerase and 2,4-dienyl-CoA reductase in rat liver mitochondria and peroxisomes by clofibrate.

Alterations in the activities of enoyl-CoA hydratase, 3-hydroxyacyl-CoA epimerase, and 2,4-dienoyl-CoA reductase in rat liver mitochondria and peroxisomes caused by clofibrate were compared in order to clarify the metabolic pathways for unsaturated fatty acids. Our results suggest that there are two pathways for fatty acids having (a) double bond(s) at the even-numbered carbon atom(s) both in mitochondria and in peroxisomes. One is the hydratase-epimerase participating pathways, and the other is the reductase participating pathway. It is considered that the former in mitochondria occurs mainly under normal conditions, and the latter becomes significant when the degradation of fatty acids is stimulated.

Animals↗
PubMed1982

Peliosis hepatis. Report of two autopsy cases with a review of literature.

Two cases of peliosis hepatis, treated with a large dose of predonine for aplastic anemia and multiple myeloma, respectively, were reported. Case 1 showed no abnormal liver function, but, in Case 2 hepatomegaly, retention of ICG, and elevation of LDH level despite the normal serum transaminase were noted. In both cases, peliotic lesions were incidentally observed at autopsy. In Case 1, the lesion was localized in the right lobe only, but, in Case 2 the lesion was present throughout the entire lobes of the liver involving also a part of the spleen and bone marrow of the sternum. Innumerable blood-filled cavities were surrounded by incomplete reticulin or injured hepatocytes. In smaller lesions observed in Case 1, focal necrosis, hemorrhage, inflammation, and thrombi were prominent features. On the other hand, in extensive lesions, as in Case 2, a marked liver cell dissociation with sequent irregular reticulin arrangement was present. Though the etiology and peliosis hepatis is still uncertain in these cases, it was assumed that a large dose of predonine might have some correlation to the occurrence.

Anabolic Agents↗
PubMed1981

Serological and immunohistological studies on lepromatous leprosy.

Sera of patients with lepromatous leprosy were studied for the presence of a variety of antibodies and immune complexes (IC). The frequencies of heterophile, Hanganutziu-Deicher and Forssman antibodies were 61 and 43%, respectively, which were significantly higher than those in other diseases. The frequency of antibodies to cardiolipin was 89% and the frequency of rheumatoid factor was 34%. Circulating IC were demonstrated in 54% of the patients' sera by Raji-cell test and in 43% by anti-antibody inhibition test. Analyses of immunoglobulin classes of IC revealed that IgG was predominant in IC of patients with lepra reaction (LR) and IgM in patients without LR. Immune deposits were found in and between cells of dermis in skin biopsy specimens of patients with LR.

Animals↗
PubMed1980

Heterophile antibodies in Japanese patients with various diseases including infectious mononucleosis.

Sera of Japanese patients with various diseases including patients with lympho-proliferative disease similar to infectious mononucleosis (IM) were studied for the presence of heterophile antibodies. Antibodies to sheep erythrocytes were demonstrated in 9 of 19 such patients whereas similar antibodies were found only in 28 of 3570 (0.8%) patients with various other diseases. Adsorption studies revealed that these antibodies in patients with IM-like syndrome are of Hangantziu-Deicher or Forssman nature. On the other hand, Paul-Bunnell antibody characteristic for IM in Caucasians was not demonstrated in Japanese suffering from IM-like syndrome.

Animals↗
PubMed1979

Paul-Bunnell antigen in malignancies and rheumatoid arthritis.

By means of agglutination inhibition test, Paul-Bunnell (P-B) antigen was demonstrated in sera of patients with cancer (5%), lymphomas or leukemias (12.5%) and rheumatoid arthritis (RA) (1.4%) as well as in synovial fluids from RA patients (27%). In contrast, none of 124 normal human sera gave positive results. P-B antigen was also demonstrated in 3 of 12 perchloric acid extracts of cancer tissues and 2 of 11 saline extracts obtained at 100 degrees C from spleens of lymphoma-leukemia patients. Extracts of apparently normal tissues from 16 individuals gave negative results.

Antigens, Neoplasm↗