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Biomedical subjects

T Muto

Publications and source records attributed to T Muto.

At least 505 records · Page 28Linked to original sources
PubMed1989

Characterization of mRNA species related to human liver cytochrome P-450 nifedipine oxidase and the regulation of catalytic activity.

P-450NF is the major enzyme in human liver involved in the metabolism of the calcium-channel blocker nifedipine. By screening a bacteriophage lambda gt11 expression library, a cDNA clone designated NF 10 with an insert length of 2.8 kilobases (kb) was isolated. This clone was sequenced and found to be highly similar in its overlapping section with sequences of two other cDNA clones previously isolated from the same expression library, NF 25 (Beaune, P. H., Umbenhauer, D. R., Bork, R. W., Lloyd, R. S., and Guengerich, F. P. (1986) Proc. Natl. Acad. Sci. U. S. A. 83, 8064-8068) and HLp (Molowa, D. T., Schuetz, E. G., Wrighton, S. A., Watkins, P. B., Kremers, P., Mendez-Picon, G., Parker, G. A., and Guzelian, P. S. (1986) Proc. Natl. Acad. Sci. U. S. A. 83, 5311-5315). However, clone NF 10 had an extra 814 or 813 bases of 3'-noncoding sequence relative to NF 25 or HLp, respectively, and this additional sequence contained a second consensus polyadenylation signal. Specific oligonucleotides were synthesized to differentiate between these three clones at the mRNA level. Oligonucleotides specific to the protein coding region of each clone were found to hybridize to mRNAs of 2.2 and 3.0 kb in size at a ratio of approximately 10:1. The major species of hybridizable mRNA was specific to clone NF 25, and levels of this mRNA could be correlated with levels of immunochemically detectable P-450NF and nifedipine oxidase activity in individual human liver samples. In addition, an oligonucleotide specific to the 3'-noncoding region of clone NF 10 hybridized only with the 3.0-kb mRNA. We conclude that alternative use of the second polyadenylation signal present in clone NF 10 results in production of the 3.0-kb mRNA species and that a pretranslational control mechanism is primarily involved in the regulation of nifedipine oxidase activity.

Amino Acid Sequence↗
PubMed1989

Evaluation of ileal W pouch-anal anastomosis for restorative proctocolectomy.

Clinical defaecatory function, neorectoanal manometry and pouchography were assessed in 16 patients treated by restorative proctocolectomy with ileal W-reservoir. The duration after ileostomy closure was 6 to 28 months (mean 17 months). There were no operative deaths and no failures where the reservoir had to be removed. Partial anastomotic dehiscence occurred in one patient, and intestinal obstruction requiring laparotomy in two. Anastomotic stricture, which could be corrected easily by dilatation, occurred in three patients. Daily stool frequency was 4.3 +/- 1.2 at 6 months after ileostomy closure, 3.8 +/- 1.2 at 12 months, and 3.3 +/- 1.0 at 24 months. The clinical score for neorectal function gradually and steadily improved with time as well as daily stool frequency. In the manometric and pouchographic studies, mean anal canal length (3.4 +2- 0.6 cm), mean maximal anal sphincter resting pressure (57.1 +/- 9.7 cm water) and mean maximal reservoir resting pressure (4.3 +/- 2.0 cm water) tended to be less than normal controls but not significantly so. Neorectoanal inhibitory reflex disappeared completely or was greatly decreased in all patients. However, all were capable of spontaneously controlled defaecation. There was an inverse linear relationship between daily stool frequency and maximal tolerated reservoir volume (p less than 0.01). There were inverse linear relationships also between daily stool frequency and horizontal diameter of the reservoir measured on pouchography (p less than 0.05), and daily stool frequency and dilatation ratio of the reservoir (p less than 0.01). From these results, we conclude that a large and wide reservoir allows better defaecatory function.

Adenomatous Polyposis Coli↗
PubMed1989

Giant cavernous hemangioma of the liver and multiple primary malignant tumors in a patient with suspected familial inhibition of natural killer cell activity--a case report.

A woman was operated on for a nonepithelial malignant tumor of the left leg and subsequently, for an epithelial carcinoma of the right breast and a borderline malignant tumor of the right ovary. She also developed a giant cavernous hemangioma that caused disseminated intravascular coagulation syndrome, which necessitated a left trisegmentectomy of the liver. Her family history suggested a hereditary predisposition to diverse malignant neoplasms, and also to giant cavernous hemangioma of the liver. Immunological evaluation disclosed selective inhibition of natural killer cell activity. Hormonal and hereditary factors are discussed in relation to the development of multiple primary tumors and giant cavernous hemangioma of the liver.

Breast Neoplasms↗
PubMed1989

Management of patients with invasive carcinoma removed by colonoscopic polypectomy.

The management of patients with invasive carcinoma removed by colonoscopic polypectomy remains controversial. In order to assess the criteria for subsequent surgery after polypectomy, the histologic findings and outcome of 25 patients with invasive carcinomas treated by polypectomy were analyzed. Subsequent surgery was indicated when removed invasive carcinoma showed at least one of the following findings: 1) carcinoma near the surgical margin, 2) vessel invasion, 3) massive invasion, and 4) poorly differentiated adenocarcinoma. The authors considered those findings to be a risk factor for local residual carcinoma or lymph-node metastases, or both. Of 25 patients, 18 showed risk factors, with 16 receiving surgery. Only one had residual carcinoma in the lymphatic vessel of the surgical specimen. The remaining 15 had no carcinoma in the surgical specimens, however, one died of recurrent disease 55 months later. Two patients with risk factors received no surgery for various reasons. Local recurrent carcinoma developed in one 39 months later and the other had no recurrent carcinoma at autopsy. Seven patients without risk factors were adequately treated by polypectomy without recurrent disease 34 to 96 months later (average, 69 months). Consequently, of 18 patients with risk factors, 3 showed either residual carcinoma in the surgical specimens or recurrent carcinoma was found later. None of 7 patients without risk factors developed recurrent disease. We recommend that patients with risk factors be followed by surgery; however, patients without risk factors can be adequately treated by polypectomy alone.

Adenoma↗
PubMed1989

Determinations of ethanol, acetaldehyde and acetate in blood and urine during alcohol oxidation in man.

Blood and urine samples were analyzed for ethanol, acetaldehyde and acetate during alcohol oxidation in Japanese men by head space gas chromatography, following the consumption of 16 ml/kg of beer during a 20 min period. The maximum level of blood/urine ethanol was found to be 15-17 mM (20-22 mM), while that of acetaldehyde in a flusher and in non-flushers was 20 microM (52 microM) and 2-5 microM (10-13 microM), respectively. Acetate levels in these groups ranged from 0.2 mM (0.1 mM) to 0.8 mM (1.0 mM). Blood ethanol levels were dose dependent, whereas acetaldehyde and acetate levels reflected individual metabolic rates. The relative concentrations of ethanol and acetaldehyde in blood and that of acetate in alcohol metabolism could be summarized as follows: 7500 (15 mM): 1-3 (2-5 microM); 250-400 (0.5-0.8 mM) for non-flushers; and 7500 (15 mM): 5-10 (10-20 microM): 250-400 (0.5-0.8 mM) for a flusher.

Acetaldehyde↗
PubMed1989

Serial section study of colonic adenomas with special reference to minute carcinoma.

A total of 73 colonic adenomas, 20 with mild atypia, 26 with moderate atypia and 27 with focal carcinoma (severe atypia), were examined by complete serial section in order to find the distribution of various degrees of atypia in adenomas. In serial sections minute carcinomatous foci were found in 3 (15%) and 12 (46.2%) of the adenomas with mild and moderate atypia, respectively. Fourteen adenomas (51.9%) with focal carcinoma had multiple foci of minute carcinoma elsewhere. Adenomas less than 1 cm in diameter showed 27% malignancy rate in serial sections. From this observation it is postulated that the malignant potential of colonic adenomas is much higher than previously believed.

Adenoma↗
PubMed1989

[Evaluation of left atrial function by 99mTc gated blood pool scan].

99mTc gated blood pool scans were studied to assess the left atrial function. Relationship between filling time and rapid emptying time was y = 0.695 x + 109 (r = 0.761, p less than 0.05, n = 14) in LAO and LPO projections. On the other hand, relationship between slow filling and rapid emptying time was good closely (y = 0.846 x + 16.9 (p less than 0.01, r = 0.975, n = 8] in standard and retrograde acquisition. To assess the left atrial function by standard acquisition in LAO projection should be available for clinical use.

Aged↗
PubMed1989

[Outcome of adjuvant mifurol chemotherapy in patients with resected colonic carcinoma].

The effectiveness of Mifurol as an adjuvant chemotherapy in colonic cancer patients has been assessed on the basis of long-term survival at various institutions. In the curative resection cases, the outcome of administrations of MMC (0.2 mg/kg x 2) + Mifurol (6-12 mg/kg/day) (group A and MMC (0.2 mg/kg x 2)+ Tegafur (12 mg/kg/day) (group B) was compared. There was no difference in the background factors or in the total amount of the alpha rugs administrated in these two groups. The five-year survival by Kaplan-Meirer's method were 56.1% and 36.1% for group A and B, respectively, the difference being particularly remarkable in Dukes C cases.

Adult↗
PubMed1989

[Microspectrophotometric DNA analysis in ulcerative colitis with special reference to its application for cancer and dysplasia diagnosis].

The DNA content was measured by microspectrophotometry from 100 specimens in 60 patients with ulcerative colitis including six complicating carcinoma. In dysplastic 23 (77%) of 30 samples showed aneuploidy or polyploidy, whereas in non-dysplastic tissues 50 (94%) of 53 samples were diploid, the difference being statistically significant. Polyploidy was often observed in the non-dysplastic mucosa of the patients complicating cancer or dysplasia. In non-dysplastic patients all samples with inflammation showed diploidy, however 10% of samples without inflammation showed polyploidy. A good correlation was found between the incidence of polyploid cells and the grade of dysplasia. From this study it was assumed that microspectrophotometric measurement of DNA content is useful in the assessment and diagnosis of dysplasia in ulcerative colitis and can be available in the screening of high-risk patients.

Adult↗
PubMed1989

[Application of straight chain fatty acid analog IPPA [omega-(p-iodophenyl)-pentadecanoic acid] for myocardial imaging--using acute myocardial infarction model].

Application for myocardial imaging and fundamental experiments were studied using straight chain fatty acid analog IPPA [omega-(p-iodophenyl)-pentadecanoic acid]. Biodistribution of IPPA in rabbits (n = 6) shows the accumulation in liver was maintained 81.0% at 30 minutes, while the accumulations of heart, lungs and kidneys were 30.0%, 10.0% and 15.0% respectively. Especially the accumulation of heart decreased rapidly from 48.0% at 3 minutes to 30.0% at 30 minutes, reflecting the effect of beta oxidation. On the other hand, in the acute myocardial infarction mode (n = 6), with occlusion in left anterior descending coronary artery, all 6 cases showed defect images at the corresponding areas after injection of 3 mCi of IPPA. Myocardial imaging with IPPA should be useful not only for myocardial metabolic diseases (cardiomyopathy etc.) but also for ischemic heart disease.

Animals↗
PubMed1989

[Clinical features and treatment of lower intestinal hemorrhage].

We have experienced 471 patients with anal bleeding during the past seven years. The Results are as follows: 1. As for the types of disease, incidence of hemorrhoid, colorectal cancer and ulcerative colitis (UC) was high, while that of small intestinal problems was low. 2. In the cases of remarkable bleeding from the lower intestinal tract, massive or acute progressive bleeding was less frequent than expected. 3. Inflammatory diseases were the main causes of massive bleeding. Bleeding by UC was the major indication for urgent operation. In the cases with acute massive hemorrhage, basic complications were often found and the possibility of the diseases of small intestine and blood vessel disorders also should be considered. 4. At the examination of bleeding patients, it is efficient to explore the lower colon and rectum first by colonoscopy or sigmoidoscopy, for most of the bleeding lesions are found in these portions. 5. As for surgical treatment, most of colorectal cancer patients with hemorrhage are able to be operated with wait- and -see management. For UC patients, complete cure operation is possible even if they have high-dose steroid medication.

Adult↗
PubMed1989

[The significance of the extensive systematic lymphadenectomy for thoracic esophageal carcinoma].

UNLABELLED: 1. MATERIALS: One hundred and seventy nine patients with thoracic esophageal carcinoma who underwent an intrathoracic esophagectomy combined with systematic lymphadenectomy were investigated. They were roughly classified into two groups, i.e., those who received extensive lymphadenectomy in bilateral cervical and upper mediastinal regions (A group: 78 subjects), and those who underwent ordinary limited dissection of the lymph nodes in the left cervical and upper mediastinum (B group: 101 subjects). 2. RESULTS: Cumulative 5-year survival rate of advanced cancer patients was 31.3% in A and 22.5% in B, the difference being of significance (p less than 0.05). The rate of postoperative mortality showed no difference between both groups, i.e., 3.8% in A and 4.9% in B groups. However, the incidence of postoperative pulmonary complication was 17.9% in A and 13.9% in B. Recurrent nerve palsy developed in frequencies of 39.7% and 17.8% in A and B groups respectively. 3. CONCLUSION: The degree of extended lymph node metastasis in carcinoma of the thoracic esophagus was closely correlated with its prognosis, and hence it is extremely important to perform intrathoracic esophagectomy with extensive systematic lymphadenectomy aiming at the favorable result in surgical treatment. It is also required to take preventive measures against postoperative complications.

Esophageal Neoplasms↗
PubMed1989

[A case of metastatic lung tumor mimicking left atrial myxoma by echocardiography].

We report a case of lung metastatic tumor originated from submandibular osteosarcoma, which shows rare advancement and findings. This lung metastatic tumor advanced from left lower lung to left atrium through the left lower pulmonary vein, which penetrated to the left ventricle in the diastolic phase mimicking left atrial myxoma. 67Ga-citrate images showed that hot accumulation were recognized behind the heart in planar image and clear advancement from the lung to the heart in the SPECT image. 67Ga-citrate SPECT image should be available for cardiac tumors.

Adult↗
PubMed1988

Intraoperative irrigation and primary resection for obstructing lesions of the left colon.

The treatment of choice for left sided colonic obstruction is still controversial. We tried primary resection and anastomosis with the aid of intraoperative irrigation of the dilated proximal colon. Twenty-one cases of obstructive carcinoma of the left colon and four cases of sigmoid colon volvulus were managed using this approach. After the antegrade or retrograde intraoperative irrigation, primary resection and anastomosis without colostomy was performed in all 25 cases. There was only one case with anastomotic leakage. The satisfactory results of our present study and of the previous reports of intraoperative irrigation lead us to recommend intraoperative irrigation and primary anastomosis for left sided colonic obstruction except when the general condition of a patient is very poor or when the prognosis is expected to be poor due to the advanced spread of carcinoma.

Adult↗