Search PubMed⌕ Search

Biomedical subjects

T Murohashi

Publications and source records attributed to T Murohashi.

At least 37 records · Page 2Linked to original sources
PubMed1973

WHO cooperative studies on the phage-typing of mycobacteria. 1. Phage lysis of Czechoslovak and Italian strains of Mycobacterium tuberculosis.

Although phage-typing of some pathogenic microbes-e.g., Escherichia coli and the genera Salmonella, Staphylococcus, and Streptomyces-is in routine use, little progress has yet been made in the phage-typing of mycobacteria, chiefly because mycobacteriophages possess a polyvalence that prevents the reliable identification of individual species of mycobacterium. Furthermore, laboratories cannot compare their results because they employ different methods and culture media and also because the phages used cannot be accurately identified. It has been shown that internationally reproducible results can be obtained for M. tuberculosis by selecting suitable mycobacteriophages and sensitive culture media, and by using a standardized technique of phage-typing. Under these conditions, phage lysis may be utilized to classify strains of M. tuberculosis.

Bacteriophage Typing↗
PubMed1973

Stimulating effect of pyruvate on the growth of Mycobacterium leprae in cell-free, semisynthetic, soft agar medium.

A bacterial suspension prepared from a nodule from a patient with lepromatous leprosy was inoculated into the cell-free, semisynthetic, semiliquid agar medium M-Y 11d-modified from the authors' medium M-Y 1c by omitting glycerol and adding pyruvate-and incubated at 37 degrees C. The growth of the organism was stimulated so markedly that after about 50 weeks' incubation of the primary culture microcolonies could be seen floating in the agar layer as tiny white particles, while the medium fluid assumed a yellow tinge. These macroscopic colonies were sucked up by pipette and subjected to microscopic examination. A bacterial suspension prepared from the first subculture of this strain by centrifugation elicited the same skin reactions in leprosy patients as did the standard Mitsuda's antigen. The organism in the culture was therefore identified as M. leprae.

Agar↗
PubMed1972

An attempt to culture Mycobacterium leprae in cell-free, semisynthetic, soft agar media.

This paper describes an attempt to culture M. leprae from leprous nodules, using cell-free, semisynthetic, soft agar media, and the effects of varying the incubation temperature and the composition of the basic medium, as well as of pretreating the bacterial suspensions with alkali. The identification test carried out in leprosy patients by intradermal injection of bacillary suspensions prepared from the primary cultures and third subcultures suggested that the bacterial masses that had formed in the suspensions were composed of M. leprae.

Agar↗