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Biomedical subjects

T Monna

Publications and source records attributed to T Monna.

At least 163 records · Page 9Linked to original sources
PubMed1978

Studies on an inhibitory factor to phytohemagglutinin-induced lymphocyte transformation found in the serum of patients with various liver diseases.

When normal lymphocytes were stimulated with PHA in the presence of serum taken from patients with various liver diseases, lower lymphocyte transformation was seen in many cases. The occurrence of a serum inhibitory factor in liver diseases tends to increase with the progress of the disease from acute hepatitis to cirrhosis of the liver. No such inhibitory factor was detected in two asymptomatic HBs-antigen carriers. When the inhibitory factor was fractionated by DE-52 column chromatography, an active component was obtained and shown to have mobilities from alpha2-globulin to beta-globulin in cellulose acetate membrane electrophoresis. However, two active fractions were separated by DEAE-cellulose column chromatography with stepwise increases of the concentration of the acetate buffer and their mobilities were consistent with those of alpha1-globulin and alpha2-globulin, respectively. In 11 cases of acute hepatitis which were followed for at least 6 months, three out of four inhibitory factor positive cases developed into the chronic form and the other became a protracted case. On the other hand, six out of seven inhibitory factor negative cases completely recovered and the remaining case followed a protracted course.

Adult↗
PubMed1976

Immunological studies on drug-induced allergic hepatitis.

In many cases of drug-induced allergic hepatitis, peripheral lymphocytes were transformed by the stimulation with a given drug in the presence of autologous serum. However, when rat liver microsome fraction of soluble liver specific antigen fraction was added to the culture instead of autologous serum, the drug-induced lymphocyte transformation was more efficiently seen than autologus serum, while rat liver mitochondria fraction was less effective. On the other hand, in the cases of allergic drug eruption which did not show any liver injury, the addition of liver subcellular fractions were much less effective to induce the lymphocyte transformation than autologous serum. These results may suggest that liver subcellular component is involved in pathogenesis of drug-induced allergic hepatitis.

Adult↗
PubMed1975

Detection of hypersensitivity to drugs by lymphocyte cultures in drug-induced allergic hepatitis.

Fifty-three out of 70 patients with drug-induced liver injury showed an immunological response to the drug using either the lymphocyte transformation test or the macrophage migration inhibitory test (MI test) or both. Both the separated lymphocyte culture and the microvolume whole blood culture technique are available as assay methods. The latter method is simpler and the results of both methods are comparable. The administration of a synthetic copolymer of polyadenylic and polyuridylic acid (poly A:U) to the culture medium enhances the lymphocyte response to antigen and is therefore very useful to detect weak responses.

Adolescent↗
PubMed1975

Studies on the subpopulation and function of peripheral lymphocytes, and inhibitor to PHA stimulation existing in the serum of patients with liver disease.

In our recent report we observed the abnormal response to PHA stimulation in the peripheral lymphocytes of patients with chronic liver diseases. The present report concerns extensive studies of this earlier report; analysis of the subpopulation and function of peripheral lymphocytes in patients with liver diseases. PHA responsiveness in acute hepatitis was reduced in the acute phase and improved during the convalescent stage. In chronic liver disease, half of the patients showed a decrease in T-cell population as well as a reduced response to PHA stimulation. These phenomena were observed more frequently in HBs-antigen positive patients than in HBs-antigen negative cases. Furthermore, we found the existence of an inhibitor to PHA response in the serum of 8 cases among 19 patients with various liver diseases. In patients with such a serum inhibitor, the reactivity of the lymphocyte itself to PHA was considerably higher but the T-cell population was much more decreased than that of inhibitor negative cases.

Acute Disease↗