Biomedical subjects
Publications and source records attributed to T Mizuta.
The incidence of physeal injuries in nearly 2,000 bony injuries was 18%. They were commoner in adolescents and specifically more frequent in the upper limbs. The incidence of growth arrest was just over 1%, whereas the incidence of serious complication was less than 1%. The prognosis depends more on the site than the Salter-Harris classification. The proximal tibia is a common site for growth disturbance.
To identify the route of hepatitis C virus (HCV) transmission, we investigated the sexual transmission of HCV by examining HCV markers among spouses of patients with chronic liver disease (CLD) due to HCV. Of 83 spouses, 14 (16.9%) had elevated serum aspartate aminotransferase or alanine aminotransferase, 20 (24.1%) had detectable anti-HCV antibodies, and 17 (20.5%) had measurable HCV-RNA in serum. However, the seropositivity rate of anti-HCV antibodies (24.1%) of patients' spouses was not significantly higher than that (15.4-27.5%) of an unselected population in the same district. Ten patient-spouse pairs underwent nucleotide sequence analysis of the HCV core and envelope genes. Overall the sequence homology of 10 couples (91.1%) was not significantly higher than that of 10 randomly chosen unrelated pairs (88.2%). As reported earlier, in an age and sex matched case-control study of HCV transmission, a history of surgery is a prominent HCV risk factor. These results suggest that sexual transmission of HCV is rare.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Receptors for peanut agglutinin (PNA) were isolated from Lewis lung carcinoma cells (3LL) by detergent solubilization and affinity chromatography on PNA-agarose. The isolated receptors showed heterogeneous yet distinct molecular species when they were analyzed by SDS gel electrophoresis. In vivo inoculation of the isolated receptors could induce potent cytotoxic effector cells against 3LL cells, which were detected by Winn's tumor neutralization assay. The PNA receptors were also effective in preventing the settlement of the intravenously injected 3LL tumors in the lung. These findings suggest that PNA receptors can be used for immunological therapy of certain cancers.
BACKGROUND AND METHODS: On the assumption that lung mast cells might respond and release histamine after lung transplantation, the number of mast cells and tissue histamine content were investigated for 2 weeks after rat orthotopic left lung isotransplantation (Lewis rat to Lewis rat) and allotransplantation (Lewis rat to Brown Norway rat). The allografts were rejected histologically by day 7 (grade A4). RESULTS: In the isografts, both the numbers of mast cells and histamine content were lower (p < 0.05) on day 4 but higher (p < 0.001) on day 14 compared with the nontransplanted donor left lungs (control). In the allografts, the numbers of mast cells on day 7 and histamine content on day 1 were lower (p < 0.05) than controls and both continued to be low thereafter. On day 7, the histamine content of the allografts was low (p < 0.05) compared with the level of the isografts. Conversely, no significant change was found in histamine content of the untransplanted right native lungs except for on day 14 after isotransplantation, when the content was higher than the controls, suggesting the possibility of a systemic signal for proliferation of mast cells. CONCLUSIONS: Our data indicated that degranulation of the pulmonary mast cells and consequent histamine release localized in the grafts occurred under the microenvironment of reimplantation and rejection after rat lung transplantation.
We assessed the effect of granulocyte apheresis in patients exhibiting increased granulocyte-to-lymphocyte ratio in order to overcome granulocytosis occurring in the terminal stages of malignancies. 17 patients with post-operative recurrent metastatic tumors including 6 gastric, 3 colonic, 2 rectal, 1 esophageal and 5 breast cancers were selected. The granulocytapheresis was performed by extracorporeal vein-to-vein circulation equipped with an apheresis column filled with cellulose acetate beads. Each week the patients underwent one or two sessions of treatment that lasted 30 to 50 minutes per session at a flow rate of 30 to 50 ml/min. 15 sessions formed 1 therapeutic cycle. The effect of granulocytapheresis resulted in partial response (PR) in 4 cases, no change (NC) in 7 cases and partial disease (PD) in 6 cases. The performance status showed 30% remission. None of the patients exhibited significant side effects. Since the treatment demonstrated anti-tumor effects, granulocytapheresis may be applied during combined cancer treatments.
To test a hypothesis that reduction in pulmonary perfusion pressure and flow affect underlying vascular disease, pulmonary pathology was studied in monocrotaline-treated rats undergoing single lung transplantation. Inbred rats were treated with 40 mg/kg (group T1, n = 6) and 80 mg/kg of monocrotaline (group T2, n = 9), received a left lung isograft 2 and 4 weeks after medication, and were killed 4 and 6 weeks after single lung transplantation, respectively. For each group, rats receiving the same amount of monocrotaline (M1, M2) or vehicle (N1, N2) served as controls. Monocrotaline-treated rats developed pulmonary vascular disease and right heart failure, resulting in severe exercise intolerance in M1 or death in M2 unless single lung transplantation had been carried out. At death, pulmonary blood flow was directed toward the left lung isograft, and the retained right lung received a significantly reduced fraction of cardiac output. Right to left ventricular weight ratio was significantly reduced in both groups as compared to the respective control rats, suggesting reduced perfusion pressure. Although thickness of media in small pulmonary arteries (media/radius) was normal (34% +/- 4%) in the lung isografts, it was significantly increased in the contralateral lung (group T1, 45% +/- 5%; group T2, 48% +/- 3%), which was not significantly different from that of monocrotaline-treated control rats, respectively (group M1, 47% +/- 7%; group M2, 49% +/- 6%). Although single lung transplantation reduced perfusion pressure and flow toward the monocrotaline-treated native lung, it failed to affect vascular morphology significantly.