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Biomedical subjects

T Miyoshi

Publications and source records attributed to T Miyoshi.

At least 109 records · Page 6Linked to original sources
PubMed1994

Tumor blood flow measured using dynamic computed tomography.

RATIONALE AND OBJECTIVES: A method for measuring the tumor blood flow (TBF) by means of dynamic computed tomography (DCT) was devised and investigated. METHODS: Twenty-seven patients with superficial tumors underwent measurement of TBF by the new DCT method. For all of the 27 patients, the thermal clearance method was employed to measure the relative tumor blood flow (RTBF). For 16 patients, TBF was measured by oxygen-15-gas positron-emission tomography (PET). The TBF values measured by DCT were compared with the RTBF by the thermal clearance method and the TBF by PET. RESULTS: The results demonstrated a linear relationship. CONCLUSIONS: The newly devised DCT method yields reliable data in measuring TBF.

Blood Volume↗
PubMed1994

Characterization of region Ic in site I on human serum albumin. Microenvironmental analysis using fluorescence spectroscopy.

Characteristics of region Ic among at least three overlapping binding regions (regions Ia, Ib and Ic) in site I on human serum albumin (HSA) were analysed using n-alkyl p-aminobenzoates (n-alkyl p-ABEs), all of which are specific fluorescent probes for region Ic. In the interaction processes between n-alkyl p-ABEs and HSA, hydrophobic interaction, van der Waals interaction and local structural changes in region Ic were found to be involved based on the results obtained by analyses of the fluorescence spectra, structure-activity relationships and thermodynamic parameters. In addition, comparison of the fluorescence spectra of n-alkyl p-ABEs in HSA and detergents indicated that the possibility of a hydrophobic region Ic around which an amino acid with cationic charge locates could not be denied because of the similarity of fluorescence spectra between n-alkyl p-ABEs in HSA and in neutral and cationic detergents. The deviation of n-alkyl p-ABEs with long alkyl chains (C9-C12) in the relationships between association constants and physicochemical properties of a series of n-alkyl p-ABEs (C1-C12) suggested that region Ic possess an optimal depth. A conformational change of HSA with increasing pH (pH 6-9) generated an increase in hydrophobicity and adaptability of the binding region and made interaction easy, with an increase in adaptability of the binding region Ic; consequently, it enhanced the binding of n-alkyl p-ABEs to HSA.

Binding Sites↗
PubMed1994

Assessment of the mechanical properties of peripheral arteries in chain saw operators using a photosensor.

In order to evaluate the severity of peripheral vascular disorders, and to detect changes in the digital arteries of workers who use chain saws, the mechanical properties of the digital arteries of 22 such workers were examined using a photosensor. The elastic properties and the nonlinearity of digital arteries were assessed through observation of the changes in the vascular volume ratio (V/Vo) against the volume (Vo) at mean blood pressure (Pm). A significant negative correlation was found between age and V/Vo at a transmural pressure of 30 mmHg (V30/Vo), which suggested that V30/Vo tended to decrease with aging. Although it was impossible to calculate V30/Vo for chain saw operators because of the vague changes in the volume pulse wave, this nonlinearity was more clearly observed through changes in the relative vascular volume (V/V40) along with a reduction in cuff pressure (Pc) to 40 mmHg below systolic blood pressure. A group of similarly aged healthy controls and three groups of workers with a different severity of vibration-induced white finger (VWF), i.e., no symptoms of VWF, mild VWF and severe VWF, were classified according to the changes in their V/V40 and the reduction in Pc. The results obtained in the present study suggest that the peripheral vascular disorders in workers using chain saws are due to the opening disturbance of the arteries with nonlinear changes in the peripheral artery, which is related to functional and organic abnormality of the arteries.

Adult↗
PubMed1994

A carcinoma in situ arising in an endocervical polyp--a case report.

A rare case of a carcinoma in situ arising from an endocervical polyp is described. The covering epithelium of the polyp transformed gradually into a carcinoma in situ via squamous metaplasia and dysplasia, spreading horizontally with glandular involvement, and colliding with the surface columnar cells. The lack of tumor tissue in the pedicle and adjacent cervical region confirms the case was a carcinoma in situ originating from the surface epithelium of the endocervical polyp.

Adenomatous Polyps↗
PubMed1994

Epidemiological study on relationship between breast cancer mortality and dietary factors.

To elucidate the effects of nutritional factors on regional differences in breast cancer mortality in Japan, the statistical analysis was made using the results of the national nutrition surveys in 12 districts from 1966 to 1980 and age-adjusted mortality from breast cancer from 1966 to 1990. The correlation analysis between food or nutrient intake and breast cancer mortality was performed with a fixed time lag of 10 years. Dietary factors such as wheat, milk, meats, animal fat, animal protein, total fat, saturated fatty acid (SF) and monounsaturated fatty acid (MUF) were correlated highly positively with breast cancer mortality. The correlation analysis between increase rates in age-adjusted mortality from breast cancer and increase rates in food or nutrient intake was also examined. Highly positive correlations were obtained between breast cancer mortality and following dietary factors; oils and fats, fruits, milk, total fat, vegetable fat, SF, MUF and n-6 polyunsaturated fatty acid. Factor analysis using fat intake by 12 districts revealed the factor representing the intake of fat component resulting from ingestion of animal fat. This factor showed a positive correlation with age-adjusted mortality from breast cancer. The findings suggested that the dietary factors due to intake of Westernized meals, especially SF had the effect on breast cancer mortality in Japan.

Adult↗
PubMed1994

Epidemiological studies on regional differences in suicide mortality and its correlation with socioeconomic factors.

To investigate regional differences in suicide mortality and its correlation with socioeconomic factors during the 1975-1990 period, we analyzed annual change in suicide mortality and 18 socioeconomic indicators among each prefectures in Japan. Annual course of age-adjusted mortality from suicide during the 1975-1985 period differed between males (increasing) and females (decreasing), because of rapidly increase in suicide mortality for middle-aged males. During the 1986-1990 period, suicide mortality decreased in both males and females. A census characteristic that suicide mortality was higher in rural districts than in urban districts intensified annually until 1985 and weakened thereafter. The factor analysis of socioeconomic indicators revealed that 1st factor was recognized as the industrial and economic activity. A high inverse correlation was observed between age-adjusted mortality from suicide and the 1st factor for males and females. In males this tendency continued until the end of the 1980s. On the whole, correlation coefficients between age-adjusted mortality from suicide and the socioeconomic indicators in males were much higher than that in females. The indicators whose annual change differed remarkably between the prefectures with an increase and a decrease in suicide mortality for males were amount of savings and frequency of bankruptcy. Therefore they were considered to be closely related to suicide mortality.

Adolescent↗
PubMed1994

Relationship between physical exercise and health of the elderly people in a rural community.

The significance of life style and regular physical exercise for health of elderly people in a rural community was evaluated by questionnaires. The subjects studied were a group who had been playing gate ball (such as croquet) for more than three years (N = 86) and a group who had not (N = 255). Significantly more males in the group playing gate ball (GB group) than in the group not playing gate ball (n-GB group) reported feeling healthier than three years before. The average overall score for life style which indicates a healthier life style was analyzed. The score for life style of males was significantly lower in the GB group than in the n-GB group. Significantly more males in the GB group did not take snacks or smoke than those in the n-GB group. Regarding regular physical exercise, significantly fewer females in the GB group went for a daily walk than those in the n-GB group. The average score for integrated regular physical exercise was significantly higher in the GB group than in the n-GB group. The scores for regular physical exercise of females in the GB group, even in spite of disability or hypertension, were significantly higher than those in the n-GB group. From this survey it appears that regular physical exercise including gate ball may be beneficial for health of elderly people in a rural community.

Aged↗
PubMed1994

Evaluation of the effects of various factors on the serum triglyceride level in young adults.

The life style of young adults has been receiving attention with a view to its improvement to preventing coronary heart diseases (CHD) in later life. In this study, for determining the influence of different life styles on the serum triglyceride level, we carried out surveys and laboratory studies on the relationships of the nutritional intake, physical activity, and cigarette smoking and alcohol consumption of young adults with their serum triglyceride levels. The nutritional survey indicated a significant correlation between the serum triglyceride level and carbohydrate intake (p < 0.01). In the survey of physical activity, a significant inverse correlation was found between the energy expenditure per kg body weight and the serum triglyceride level (p < 0.05). No significant relationship was found of smoking or drinking with the serum triglyceride level. The body mass index was found to have effects on both the serum triglyceride and total cholesterol levels. Of the factors examined, carbohydrate intake and energy expenditure per kg body weight had the greatest effects on the serum triglyceride level. Considering the trend for young people to consume large quantities of carbonated drinks, in which most of carbohydrate is sucrose, we tested the affect of a high carbohydrate diet on one group of subjects and found that it caused a significant increase in the serum triglyceride level (p < 0.05). Another group for whom a mild exercise regimen was prescribed showed slight, but not significant decrease in the serum triglyceride level. These results suggest that at least optimal nutrition and physical activity including weight control during adolescence are important for preventing CHD.

Adult↗
PubMed1994

Studies on in vitro paraquat and diquat removal by activated carbon.

The adsorption characteristics of paraquat and diquat onto activated carbon in vitro were discussed for the primary treatment of acute poisoning by accidental, suicidal or homicidal ingestion of paraquat containing herbicides. Paraquat was adsorbed onto activated carbon more abundantly and more rapidly in physiological saline solution than that in artificial gastric juice and distilled water. Most suitable solvent for paraquat removal by activated carbon was physiological saline solution (0.9% sodium chloride solution). No significant correlation was observed between the ability of paraquat removal and the properties of adsorbent. Paraquat was preferentially adsorbed onto activated carbon in the mixed solution. The adsorption abilities by activated carbon (the removal ratio, the amount adsorbed and the adsorption rate) for paraquat were larger than those for diquat, and it was enhanced by added sodium chloride and added magnesium sulfate. Enhancing effect for adsorption removal was proportional to the saline concentration. As addition of salts into carbon suspension enhanced the adsorption ability, it will contribute to the effective treatment of acute poisoning.

Adsorption↗
PubMed1994

[A case of chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) with bilateral recurrent nerve palsy and primary alveolar hypoventilation--comparative studies of the histological findings of the two sural nerve biopsies with 9 years interval].

A 27-year-old man noticed a tingling sensation in his fingers and toes in early 1982, at the age of 18 years, and his symptom gradually progressed in the following several months. In December 1982, based on neurological and laboratory examinations including the first sural nerve biopsy on the right side, a diagnosis of axonal sensory neuropathy of unknown etiology was made. During the following years, muscle weakness in the distal limbs and hoarseness developed and progressed, and the sensory impairments became gradually evident. On the second neurological examination in July 1991, he showed soft palate palsy and bilateral recurrent nerve palsy. The intrinsic muscles of hands and feet were atrophic, and pes cavus was noted bilaterally. There was mild to moderate weakness in the distal muscles of both limbs. Ankle jerk was absent and other tendon reflexes were decreased in both limbs. A mild to moderate decrease of both superficial and deep sensations with mild paresthesia was noted in both hands and feet. Routine laboratory findings were unremarkable. In blood gas analysis, hypercapnea and respiratory acidosis were found. Spirometry showed an increase of residual volume, and alveolar CO2-pulmonary ventilation response test suggested the presence of primary alveolar hypoventilation caused by hypofunction of the medullary respiratory center. In nerve conduction studies, motor nerve conduction velocities were moderately reduced in bilateral median and ulnar nerves. Distal latencies of M-waves were prolonged in bilateral median nerves. Temporal dispersion of M-wave was found in the left tibial nerve. The amplitudes of sensory action potentials were moderately reduced in bilateral median and sural nerves.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1994

Neonatal group B streptococcal sepsis during 2 years of a universal screening program.

OBJECTIVE: To assess the feasibility and efficacy of a protocol for universal screening for group B streptococci combined with selective intrapartum prophylaxis at a teaching hospital. METHODS: This is a descriptive study of experience with a standardized protocol in which patients were screened at 26-28 weeks with a rectal and genital culture placed directly in selective media. As risk factors, we used clinical chorioamnionitis, preterm birth, and rupture of the membranes greater than 12 hours. Participants were all women receiving prenatal care at our hospital. Major outcomes were compliance and neonatal sepsis due to group B streptococci. RESULTS: The prevalence of rectal and genital group B streptococci was 18.5% of 3721 screened women. Of culture-positive women, 35% developed risk factors (9% chorioamnionitis, 13% preterm birth, and 13% membrane rupture greater than 12 hours at term). With strict application of criteria, the compliance rate in administering indicated prophylaxis was 80.3%. Of women receiving prophylaxis, 42% had the first dose for 4 hours or less before delivery. There were five cases of group B streptococcal neonatal sepsis, resulting from either protocol violations, protocol failures, or both. Compared to the historic rate of group B streptococcal sepsis of 1.5 per 1000 births at our hospital, the rate in these 2 years was 1.0 per 1000 (1.6 per 1000 in the first year and 0.5 per 1000 in the second). CONCLUSIONS: It is feasible to conduct such a protocol, but compliance is only moderately good because the algorithm is complex. The protocol is not foolproof in preventing neonatal group B streptococcal sepsis, as there are protocol failures and violations.

Adult↗
PubMed1993

Isolation of cDNA encoding a binding protein specific to 5'-phosphorylated single-stranded DNA with G-rich sequences.

We have isolated the cDNA encoding a binding protein to the sequence motif of the immunoglobulin S mu region by the southwestern method. The binding protein designated S mu bp-2 specifically binds to 5'-phosphorylated single-stranded DNA containing 5'-G and GGGG stretches. The amino acid sequence deduced from the cDNA sequence showed that the S mu bp-2 belongs to the putative helicase superfamily which is involved in replication, recombination and repair. Expression of S mu bp-2 mRNA is ubiquitous and augmented in spleen cells stimulated with lipopolysaccharide and interleukin 4 which also induce class switching. The S mu bp-2 gene is conserved among vertebrates. Possible involvement of S mu bp-2 in class switching is discussed.

Amino Acid Sequence↗
PubMed1993

Binding of suprofen to human serum albumin. Role of the suprofen carboxyl group.

The binding of suprofen (SP), a non-steroidal anti-inflammatory drug of the arylpropionic acid class, and its methyl ester derivative (SPM) to human serum albumin (HSA) was studied by dialysis and spectroscopic techniques. In spite of the remarkable differences in the physicochemical properties of SP and SPM, the binding of each molecule to HSA was quantitatively very similar. Thermodynamic analysis suggests that the interaction of SP with HSA may be caused by electrostatic as well as hydrophobic forces, whereas the interactions with SPM may be explained by hydrophobic and van der Waals forces. Similarities in the difference UV absorption spectra between ligand-detergent micelle and -HSA systems indicate that the SP and SPM molecules are inserted into a hydrophobic crevice on HSA. The same studies suggest that the carboxyl group of SP interacts with a cationic sub-site which is closely associated with the SP binding site. Proton relaxation rate measurements indicate that the thiophen ring and propanoate portion of the SP molecule is the major binding site for HSA. The locations of SP and SPM binding sites were identified by using fluorescence probes which bind to a known site on HSA. The displacement data implied that SP primarily binds to Site II, while the high affinity site of SPM as well as low affinity site of SP are at the warfarin binding site in the Site I area. From binding data with chemically modified HSA derivatives, it is likely that highly reactive tyrosine (Tyr) and lysine (Lys) residues, which may be Tyr-411 and Lys-195, are specifically involved in SP binding. In contrast, these two residues are clearly separated from the SPM binding site. The binding of SP and SPM is independent of conformational changes on HSA that accompany N-B transition. There is evidence that the carboxyl group may play a crucial role in the high affinity binding processes of SP to HSA.

2-Hydroxy-5-nitrobenzyl Bromide↗
PubMed1993

Comparative studies of larval and pupal phenoloxidase of the housefly, Musca domestica L.

1. Larval and pupal phenoloxidase of the housefly, Musca domestica L. resembled each other in some enzymatic properties, such as optimal pH, pH stability, optimal temperature and thermal stability. 2. The pI values of both enzymes were 4.85, as determined by isoelectric focusing. The molecular weights of two enzymes had a similar value (320,000 and 330,000 for larval and pupal phenoloxidase, respectively) and the molecular weights of these subunits had the same value of 60,000. 3. The aminio acid compositions of two enzymes were very similar. N-terminal amino acid sequences of larval and pupal enzymes were Glu-Glu-Ala-Thr-Val-Val-Pro-Asp-Gly- and Ala-Thr-Val-Val-Pro-Asp-Gly-Tyr-Phe-Met-, respectively, and the residues 3-9 (larval phenoloxidase) were in agreement with the residues 1-7 (pupal phenoloxidase).

Amino Acid Sequence↗
PubMed1993

Study on hydrophilic properties of gelatin as a clinical wound dressing. I. Hydrophilic properties of gelatin as a wound dressing.

A basic study on hydrophilicity of gelatin was made of the fitness, water-absorbing ability, water-holding ability, affinity and stability of included water in order to utilize it as a protective covering for medical use. Gelatin No. 3, being highly viscous and having a lower jelly strength, was strongly viscous and flexible, and thus, it stuck close to the surface of a lesioned site such as a wound or a burn. Since the melting point of a gelatin containing 50% water was in the range of 43 to 45 degrees C, the gelatin will be fully used as a protective covering for a wound. Below 20% relative humidity, water molecules were bound to the polar amino acid side chains of gelatin and the bound water caused a swelling of the gelatin, which is maintained by capillary sorption of water into its collagen-like four-dimensional structure. The amount of water sorbed at a relative humidity of 80% at 11 degrees C was higher by 50 to 80 mg/g compared to that at 37 degrees C. Gelatin No. 3 had a very high water-holding ability and it was very hard for it to release the sorbed water at a relative humidity of 80 to 90%. Water molecules were bound to the strong active sites of the gelatin at low relative humidity. The gelatin swelled and then, newly active sites appeared on the surface of the gelatin. The hydrophilic activity decreased with an increase in moisture content, whereas the stability of water molecules in the gelatin increased.(ABSTRACT TRUNCATED AT 250 WORDS)

Absorption↗
PubMed1993

Study on hydrophilic properties of gelatin as a clinical wound dressing. II. Water-absorbing property and hemostatic effect of gelatin.

For clinical use of gelatin as a dressing, we determined the water-absorbing capacity of gelatin for isotonic sodium chloride solution (ISCS) and bovine serum albumin-isotonic sodium chloride solution (BSA-ISCS). There was no significant difference between water absorption of gelatin with ISCS and with BSA-ISCS. The water-absorbing capacity of gelatin increased with increasing viscosity: gelatins No. 1 > No. 2 > No. 3. Enormous spaces of 20 to 30 microns or approximately 10 microns in size were observed on the gelatin surface. Scaly materials of 10 to 20 microns in size were also observed. The pores of 10 A in radius were predominant in all the gelatins studied. Since the sizes of cellular blood components range between 2 and 17 microns, they can enter the spaces but not the pores of 10 A in radius. On the other hand, water molecules, the major component of plasma, can enter the pores. Of theoretical sorption isotherms, Henderson's and Chung-Pfost's equations were fitted within the range of relative humidity between 1.5 and 75%. Gelatin No. 1 had the smallest constants n, K, B, and C followed by gelatins No. 2 and No. 3, suggesting that gelatin No. 1 is the most flexible for water and thus is fitted most easily to a raw area such as a wound or burn surface. The study of hemostatic effects of gelatin in rats demonstrated that when used at concentrations more than 50% of the bleeding amount, gelatin absorbed water from blood, and was stuck to and covered the wound surface.

Absorption↗