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Biomedical subjects

T Miyoshi

Publications and source records attributed to T Miyoshi.

At least 217 records · Page 12Linked to original sources
PubMed1987

[Second look operations in ovarian cancer--a clinical study on the detection of recurrent tumors and the judgment on termination of treatment in 84 cases].

Various investigations were conducted on 84 patients undergoing a second look operation (S.L.O.) intended to detect any recurrent tumors and determine the timing of the cessation of therapy. The following conclusions were obtained: Tumors were found in 18 patients (21.4%) whose preoperative diagnosis was neither subjectively nor objectively abnormal. Patients who received S.L.O. had a considerably better 5 year survival rate than the control group. S.L.O. can safely be omitted only in patients with Stage I border line ovarian tumors. S.L.O. is essential in cases where basic surgical procedures were not carried out during the initial operation. No occlusion could be reached as to the best time to perform S.L.O. In almost all of the patients who died, the site of recurrence was somewhere outside the abdominal cavity, but no recurrent tumors had been found during S.L.O. S.L.O. is still a more efficient follow-up method than CA125, TPA or IAP tumor markers, although the preoperative results of these tests reflected the laparotomy findings relatively well.

Adult↗
PubMed1986

Human purified interleukin-1 inhibits DNA synthesis and cell growth of osteoblastic cell line (MC3T3-E1), but enhances alkaline phosphatase activity in the cells.

We examined the effects of human purified interleukin-1 (IL-1) on DNA synthesis, cell growth, and alkaline phosphatase activity in the osteoblastic cell line MC3T3-E1 under both preconfluent and confluent culture conditions. Addition of IL-1 to the cells markedly inhibited their DNA synthesis and growth over the range 1-10 U/ml. Such significant inhibitory effects were observed in cells cultivated in 1 or 5% fetal calf serum (FCS)-containing alpha modification Eagle's medium (alpha-MEM), but not in alpha-MEM containing 10% FCS. In contrast, alkaline phosphatase activity was enhanced significantly by IL-1 in the cell line cultivated in 1% FCS-containing alpha-MEM. These results demonstrate that human purified IL-1 is effective in inducing the differentiation of osteoblastic cell MC3T3-E1.

Alkaline Phosphatase↗
PubMed1986

Humoral antibody response against Bacteroides gingivalis-specific antigen recognized by monoclonal antibody in adult periodontal patients.

An antigen recognized by monoclonal antibody specific for Bacteroides gingivalis was purified in the presence of 0.5% (wt/vol) beta-octyl-glucoside by immunoadsorbent column chromatography. The purified antigen was homogeneous as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and silver staining, and the pattern of SDS-PAGE agreed with that of immunoblotting. The molecule exhibited an apparent molecular weight of about 62,000 by SDS-PAGE. The antigen was sensitive to trypsin, Staphylococcus aureus V8 protease, DNase I and II, and heating, but insensitive to RNase and neuraminidase. By the enzyme-linked immunosorbent assay method, the purified antigen was not cross-reactive with rat polyclonal antibodies to each of several black-pigmented Bacteroides species, Fusobacterium nucleatum, Eikenella corrodens, and Actinobacillus actinomycetemcomitans. These results indicate that the purified antigen is specific for B. gingivalis. Humoral antibody titers in adult periodontal patients against the specific antigen were measured by the enzyme-linked immunosorbent assay. Serum immunoglobulin G antibody titers against the specific antigen in adult periodontal patients correlated significantly with the severity of periodontal disease. However, such significant correlation was not observed with serum immunoglobulin M antibody titers.

Adult↗
PubMed1986

Effect of high carbohydrate diet on serum gamma-glutamyl transpeptidase fraction pattern in Japanese young men.

Changes in the activity of serum gamma-glutamyl transpeptidase (gamma-GTP) and the percentage of the gamma-GTP fraction in healthy young men given a high carbohydrate diet (480-636 g/day, 80% of the total energy) for 21 days were examined. Serum total gamma-GTP activity showed no significant change in four healthy young volunteers who received high carbohydrate diet for 21 days. However, the percentage of the gamma-GTP (1) fraction increased significantly (P less than 0.01) from the basal level of 55.6 +/- 4.0% to 67.6 +/- 0.9% on day 10, and then decreased to 58.4 +/- 1.4% on day 21. When the experimental diet was replaced by usual diet, the percentage of the gamma-GTP (1) fraction returned to the same level as before the experiment. It is concluded from the results that the nutrient intake affects the percentage of gamma-GTP (1), but not the total serum gamma-GTP activity.

Adult↗
PubMed1986

Effect of high carbohydrate diet on serum lactate dehydrogenase isozyme pattern in Japanese young men.

Changes in the activity of serum lactate dehydrogenase (LDH) and the serum LDH isozyme pattern in healthy young men given a high carbohydrate diet (480-636 g/day, 80% of the total energy) for 21 days were examined. Serum total LDH activity showed no significant change in four healthy young volunteers who received high carbohydrate diet for 21 days. However, the percentage of LDH-4 increased significantly (P less than 0.01) from 8.5 +/- 2.4 to 10.9 +/- 1.9% after 21 days, as did also the percentage of LDH-5 (P less than 0.01) from 5.1 +/- 1.9 to 10.7 +/- 2.9%. The percentage of M-type LDH activity increased 30% during the experiment (P less than 0.05). It is concluded from the results that the high carbohydrate intake affects the percentage of LDH-4 and LDH-5, but not the total serum LDH activity.

Adult↗
PubMed1986

[Study on cefotiam in the perinatal period].

Cefotiam (CTM), a new cephem antibiotic with high activity against Gram-positive and Gram-negative bacteria, has been investigated for use in 60 mothers in perinatal period, and following results were obtained. The concentration of CTM in maternal serum was 38 micrograms/ml at 0.5 hour after an intravenous administration of 1 g. A good transport of CTM into umbilical cord serum and amniotic fluid was observed after the intravenous administration into the mother. No adverse effect appeared in the neonates. The CTM is highly useful antibiotic in perinatal infections, and the safe dose of CTM to the mother in perinatal period is considered to be 1-2 g per day.

Abortion, Induced↗
PubMed1986

[A study on ceftazidime in the perinatal period].

Ceftazidime (CAZ), a new cephem antibiotic with high activity against Gram-negative bacteria, has been investigated for use in mothers in perinatal period, and the results obtained are summarized below. Into maternal serum, umbilical cord serum and amniotic fluid, CAZ showed good transfer after intravenous administration of 1 g or 2 g into mothers, and no adverse effect appeared in their neonates. The CAZ is a very useful antibiotic for the prophylaxis and the treatment of perinatal infections at a dose level of 1-2 g per day.

Bacterial Infections↗
PubMed1986

Indium-111 leukocyte imaging in patients with rheumatoid arthritis.

This study evaluates the usefulness of labeled leukocyte imaging in patients with rheumatoid arthritis. In 33 patients, the incidence of pain and swelling in 66 wrist joints and 66 knee joints was compared with the accumulation of [111In]leukocytes. No accumulation of [111In]leukocytes was seen in any of the patients' wrists (0/12) or knee joints (0/14) when both pain and swelling were absent. In contrast, 93% (25/27) of wrist joints and 80% (24/30) of knee joints with both pain and swelling were positive by [111In]leukocyte scintigraphy. There was little correlation between the stage of the disease, as determined by radiography, and [111In]leukocyte accumulation. This study suggests that [111In]leukocyte imaging may be a reliable procedure for monitoring the activity of rheumatoid arthritis, especially for confirming the lack of an ongoing inflammatory response.

Adult↗
PubMed1986

[An experimental study of cisplatin and it's antidote, sodium thiosulfate, in ovarian pseudomyxoma implanted into nude mice abdominal cavity].

While Cisplatin was found to be effective in treating pseudomyxoma, its side effects, particularly on the kidneys, were very strong. Recently a "two channel therapy", in which it is used along with sodiumthiosulfate, has been reported to minimize the side effects. The authors used nude mice with pseudomyxoma implanted into the abdominal cavity in an experiment in which Cisplatin was introduced into the abdominal cavity and sodiumthiosulfate injected subcutaneously in a two channel procedure to determine the effect of sodiumthiosulfate on the action of Cisplatin on the pseudomyxoma and on the side effects of Cisplatin. The following results were obtained: The survival rate was improved when sodiumthiosulfate was administered with 40 mg/kg and 20 mg/kg of Cisplatin. Body weight loss was controlled when sodiumthiosulfate was administered with 20 mg/kg of Cisplatin. On the other hand, the histological effect of Cisplatin was also suppressed when sodiumthiosulfate was co-administered. In this study, we failed to observed any histological changes in the kidneys and liver attributable to Cisplatin.

Animals↗