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Biomedical subjects

T Masuda

Publications and source records attributed to T Masuda.

At least 685 records · Page 38Linked to original sources
PubMed1985

[An autopsy case of sudden death by obstruction of the pulmonary artery in cardiac rhabdomyosarcoma].

The patient was a 50-year-old man. Chest X-ray films showed a tumor shadow near the heart, which was diagnosed as carcinoma of the left lung. The patient's heart suddenly and unexpectedly stopped and he consequently died when his body was set in the right lateral position for operation on the left pulmonary tumor. A tumor measuring 6 X 3 X 3 cm was found in the region from the right ventricle to the pulmonary artery at autopsy. It was postulated that the tumor might have obstructed the pulmonary artery when the position of the patient's body was changed for surgery. A metastatic nodule was found in the left lung. The tumor was a rhabdomyosarcoma, the pleomorphic type.

Arterial Occlusive Diseases↗
PubMed1984

Distribution and ultrastructure of S-100-immunoreactive cells in the human thymus.

The present study deals with the localization and ultrastructure of S-100-immunoreactive cells in the human thymus. These immunoreactive cells are distributed mainly in the medulla with some scattered elements in the cortex. Electron-microscopic observation revealed that the cells are characterized by an irregularly shaped nucleus, tubulovesicular structures in the cytoplasm and characteristic interdigitations of the plasma membrane. The cells often embrace lymphocytes with their branched processes. On the basis of these morphological features, the immunostained elements were identified as interdigitating cells (IDCs). The immunocytochemistry for S-100 visualizes the precise distribution and extension of the IDCs under the light microscope and indicates that the IDCs form no structural networks such as those established by the thymic epithelial cells. Since the IDCs in human lymph nodes have also been reported to contain S-100-like immunoreactivity, S-100 protein can be regarded as a useful marker for identifying the IDCs in the human thymus and other lymphoid organs.

Adult↗
PubMed1984

A case of posttransfusion hepatitis A.

A 63-year-old female case of cancer of the colon who developed posttransfusion hepatitis A was presented. She became ill 49 days after receiving a single unit of concentrated red cells from a donor who also developed hepatitis A 10 days after donation.

Adult↗
PubMed1984

Immunological characterization of FcR gamma bearing and nonbearing B cells: functional modulation of immune complexes.

By the 1g sedimentation method using discontinuous gradients of Ficoll solution (concentrations of 6 to 14%), keyhole limpet hemocyanin (KLH)-primed spleen cells of C3H/He or DBA/2 mice were fractionated into 4 to 10 populations after IgG antibody-coated erythrocytes (EA gamma) rosetting and then treatment with anti-Thy-1.2 + complement (C). No significant difference was observed in the distribution of isotype specificities of surface immunoglobulins on B cells in each population thus fractionated, when determined by indirect immunofluorescence staining. The mixture of the 12 and 14% Ficoll fractions contained 95% of B cells bearing Fc receptor for IgG (FcR+ gamma) and 3.58% of antigen-binding cells (ABC) for KLH, while the 8% Ficoll fraction included 15% of FcR+ gamma B cells and 1.53% of ABC. Nevertheless, the FcR- gamma B-cell-enriched populations caused intensive plaque-forming cell (PFC) responses to dinitrophenol (DNP), whereas FcR+ gamma B-cell-enriched populations generated weak responses. Noteworthy is that 4 days preculture of a population containing 95% FcR+ gamma B cells resulted in the appearance of precursor activity which was ascertained by a further 4 days culture of these cells with antigen, DNP-dextran. These findings suggest that FcR gamma bearing B cells intrinsically possess precursor activity for IgM/IgG antibody-forming cells, but lose it transiently by binding immune complexes (IC). Moreover, the titer of a factor suppressing anti-DNP PFC responses (suppressive B-cell factor, SBF) was higher in the 24-hr culture supernatants of the FcR+ gamma B-cell-enriched fraction than of the FcR- gamma B-cell-enriched fraction, suggesting that SBF is produced by FcR+ gamma B cells themselves. Thus, IC seems to play an important role for the negative feedback regulation of antibody production by stimulating FcR gamma bearing B cells.

Animals↗
PubMed1984

Chemotactic subpopulation of macrophage cell line cells (M1 cells) discerned by three macrophage chemotactic factors from delayed hypersensitivity reaction sites.

The functional specificity of three types of macrophage chemotactic factors ( MCFs ), -a, -b, and -c, from purified protein derivative of tuberculin (PPD)-induced delayed hypersensitivity reaction (DHR) skin sites on guinea pigs, was analyzed using macrophage cell line cells, M1, established from myeloid leukemia cells of a SL/Am strain mouse. M1 cells yielded two subclones: Mk1 cells, which were Ia+ and migrated specifically toward MCF-c; and Mm1 cells, which were Ia- and migrated specifically toward MCF-a and -b. These differences show the heterogeneity of the biologic activities of the MCFs in the presence of cell line cells. M-1 cells, blast cells, when grown in continuous culture, migrated toward none of the MCFs . Upon differentiation to mature macrophage-like cells in conditioned medium (M+1 cells), however, they migrated to MCF-a, -b, and -c and the experimental evidence points to the possibility that M+1 cells are separated into subpopulations on the basis of their chemotactic response. Cytotoxic treatment of M+1 cells with anti-Ia antisera resulted in the specific decrease of activity toward MCF-c. Furthermore, MCF-c attracted Ia-positive M+1 cells, while MCF-a and -b attracted Ia-negative M+1 cells. Thus, our results indicate the existence of two migrating subpopulations of M+1 cells with specificities for MCF-a, and -b and for MCF-c, respectively. The data suggest that MCF-c attracts Ia-bearing accessory macrophages and MCF-a and -b attract Ia-negative macrophages in the DHR.

Animals↗
PubMed1984

Microsomal transformation of emodin into a direct mutagen.

The activation mechanism of emodin, a fungal anthraquinone and constituent of rhubarb, into a direct mutagen to Salmonella typhimurium TA1537 was investigated by using the S9 and microsomes of rat livers. Upon incubating emodin with the hepatic S9 derived from PCB-pretreated rats, this anthraquinone exhibited mutagenicity in the presence of NADPH or NADH, and this enzymatic activation, maximal at pH 7.0 and occurring in the microsomes, was induced by the pretreatment of rats with PCB, 3-methyl-cholanthrene or phenobarbital and was inhibited by alpha-naphthoflavone, SKF 525A and carbon monoxide. Thin-layer chromatographic analysis revealed that emodin was biotransformed by the microsomal enzymes into at least 5 quinonoid metabolites, among which one pigment, identified as 2-hydroxyemodin (1,2,3,8-tetrahydroxy-6-methyl-anthraquinone), was proved to be a direct mutagen to the test strain, and the remaining 4 quinoniod metabolites were negative or far less active than this active principle.

Animals↗
PubMed1984

Elimination of nitrous oxide accelerates elimination of halothane: reversed second gas effect.

The effect of nitrous oxide on the elimination of halothane was studied in 10 patients ranging in age from 20 to 50 years. After establishing a stable baseline (inspired halothane concentration: 0.85%, end-tidal halothane concentration: 0.75%), halothane administration was stopped and the rate of decrease in alveolar concentration of halothane (FE/ FE0 , FE: measured end-tidal concentration of halothane; FE0 : the endtidal concentration immediately preceding the cessation of halothane administration) was measured continuously. The rate of decrease in FE/ FE0 was more rapid when nitrous oxide (70%) is discontinued abruptly and replaced by the same concentration of nitrogen (Part 2) than when the nitrous oxide is continued (Part 1). One minute and a half after the cessation of halothane administration, FE/ FE0 was 0.38 +/- 0.05 (mean +/- SD) in Part 2 and 0.45 +/- 0.04 in Part 1 (P less than 0.01). In Part 2, the fall in the alveolar concentration of halothane was accompanied by a decrease in alveolar carbon dioxide from 4.27 +/- 0.01% to 4.16 +/- 0.01% at 1.5 min and an increase in the mean expired tidal volume from 522 +/- 39 ml to 557 +/- 29 ml. The authors conclude that the elimination of nitrous oxide accelerates the elimination of halothane both by dilution and by an increased expired ventilation.

Adult↗
PubMed1984

An immunofluorescent study of generalized infection of human cytomegalovirus in a patient with systemic lupus erythematosus.

An autopsy case of generalized infection of cytomegalovirus (CMV) in an adult with systemic lupus erythematosus is reported. CMV infection was histologically diagnosed postmortem by the presence of the characteristic cytomegalic cells with inclusion body. In the lungs, viral antigen was seen not only in the cytomegalic cells with inclusion body but also in the cells without intranuclear inclusion body in both of the frozen and the formalin-fixed paraffin sections. Viral antigen was also detected in frozen sections of the liver. CMV was isolated from the lung and liver.

Adult↗
PubMed1984

Migration of bone marrow lymphocytes in mice: immunofluorescent staining using antiallotype serum.

Bone marrow cells from CSW (Igha) mice were injected intravenously into the congeneic CWB (Ighb) mice. The lymphoid tissues of the recipient CWB mice were examined for donor type surface immunoglobulin (SIg)-positive cells using fluorescein isothiocyanate-conjugated antiallotype serum Ighb anti-Igha. Donor type SIg-positive cells were rarely found in the recipients immediately after injection. However, the donor type B cells increased in the recipient's spleen from days 1-2 and reached a plateau thereafter. The present method provides a useful means for studying the migration patterns and differentiation of bone marrow lymphocytes.

Animals↗
PubMed1984

Glycosaminoglycans of human colonic mucosa and colonic adenocarcinoma.

The mucosal scrapings of the normal human colons and the colonic adenocarcinoma tissues were digested with pronase, and the glycosaminoglycan fractions were prepared by fractionation with cetylpyridinium chloride after treatment with deoxyribonuclease. Chondroitin sulfate A/C, heparan sulfate, and hyaluronic acid were contained in the glycosaminoglycan fraction of the normal tissue, but dermatan sulfate was undetectable. In contrast, the glycosaminoglycan fractions derived from the tumors contained substantial amounts of dermatan sulfate together with the foregoing three glycosaminoglycans. The total content of glycosaminoglycans per wet tissue weight was elevated in the tumor tissues, which was consistent with histochemical findings.

Adenocarcinoma↗
PubMed1984

The OP-Rheometer system, a new device for analysis of viscosity and viscoelasticity of blood: description and clinical application.

OP-Rheometer is a rotational viscometer of concentric type, which was developed to measure blood viscosity at low shear rate (0.2-40 sec-1) and viscoelasticity at 0.1-3.0 Hz in the context of clinical medicine. This system consists of a mechanical unit, a control unit (console), a data processing unit and a printer. The dynamic or steady state flow data are automatically printed out. An outer cylinder (sample container) is connected to a torsion wire assembly supported by a pair of magnetic bearings. The angular displacement is detected by a pair of variable differential transformers. In clinical application of this rheometer, authours have confirmed that the viscosity and viscoelasticity of blood from diabetic patients are higher than for normal subjects.

Adult↗
PubMed1984

Cellular uptake and efflux and cytostatic activity of 4'-O-tetrahydropyranyladriamycin in adriamycin-sensitive and resistant tumor cell lines.

Cellular uptake and cytostatic activity of 4'-O-tetrahydropyranyladriamycin (THP) in various sublines resistant to anthracycline antibiotics of mouse lymphoblastoma L5178Y, Chinese hamster ovary (CHO) and mouse leukemia P388 cells were studied. All the sublines resistant to adriamycin (ADR) showed slightly decreased uptake of THP as compared with each sensitive lines, but THP was still taken up much more quickly than ADR by each of the ADR-resistant cell lines. Efflux of both anthracycline glycosides from the ADR-resistant P388 cells was faster than that from the ADR-sensitive P388 cells. The percentage of THP retained at equilibrium was higher than that of ADR in both ADR-resistant and -sensitive P388 cells. Cytotoxicity of THP to ADR-resistant cell lines was considerably lower compared with that for each of the sensitive lines but THP inhibited growth of ADR-resistant tumor cells at a concentration about 10 times lower than that for ADR. Thus THP was taken up more quickly, effluxed more slowly than ADR from the ADR-resistant cells, and showed stronger cytostatic activity than ADR on the cells.

Animals↗
PubMed1984

Murine IgA binding factors produced by Fc alpha R(+) T cells: role of Fc gamma R(+) cells for the induction of Fc alpha R and formation of IgA-binding factor in Con A-activated cells.

The relationship between the production of a T cell factor having affinity for IgA (IgA-binding factor(s); IgA BF) and the expression of Fc receptors specific for IgA (Fc alpha R) was studied by using murine spleen cells activated with concanavalin A (Con A blasts). Fc alpha R was detected by the cytophilic binding of anti-TNP murine IgA myeloma protein (MOPC 315 IgA) to Con A blasts as determined by an indirect rosette method with trinitrophenylated sheep red blood cells (TNP-SRBC). After 18 hr preculture with IgA, Fc alpha R was expressed on 15 to 20% of Con A blasts, which released IgA BF suppressing the in vitro IgA synthesis of the spleen cells stimulated with pokeweed mitogen (PWM). Without preculture with IgA, there was neither induction of Fc alpha R nor the production of IgA BF from Con A blasts. Fc alpha R was not induced on Con A blasts by IgA if Fc gamma R(+) cells were depleted from the blasts by rosetting with SRBC sensitized with rabbit IgG antibody (EA gamma). Even after preculture with IgA, the suppressive IgA BF was undetectable in the culture supernatant of Con A blasts depleted of the Fc gamma R(+) cell population. By using a double rosette method with EA gamma and trinitrophenylated quail red blood cells, Fc alpha R proved to be co-expressed on Fc gamma R(+) precursor T cells in the Con A blasts. The results suggested that both Fc gamma R and Fc alpha R could be co-expressed on Con A blasts, as is the case with T2D4 Fc gamma R(+), Fc alpha R(+) T hybridoma cells, which are known to produce IgG-binding factor(s) (IgG BF) and IgA BF. The relationship between Fc gamma R and Fc alpha R on a single cell was studied by using monoclonal anti-Fc gamma R antibody (2. 4G2 ). The reactivity of 2. 4G2 antibody with T cell Fc gamma R was proved by the inhibition of EA gamma rosette formation by Con A blasts or T2D4 cells. The addition of 2. 4G2 monoclonal antibody, however, did not affect the induction of Fc alpha R on Con A blasts by IgA. Furthermore, the binding of IgA to Fc alpha R already expressed on L5178Y T lymphoma cell line cells was not inhibited by the monoclonal antibody. The results confirmed that Fc alpha R are distinct from Fc gamma R co-expressed on the same Con A blasts, and that the expression of Fc alpha R on Fc gamma R(+) T cells and their production of suppressive IgA BF may be induced by the binding of IgA to Fc alpha R.

Animals↗
PubMed1984

[A case of multicentric early carcinoma of the stomach--preoperative diagnosis].

A 59-year-old man was admitted complaining of substernal pain and anorexia. Upper GI series revealed a benign gastric ulcer in the posterior wall, and gastrofiberscopy was done. Besides the benign lesion, two early carcinomas of type IIc were found. Biopsy specimens of both lesions showed malignancy and gastrectomy was performed. Histological investigation revealed poorly differentiated adenocarcinoma in the anterior wall and well differentiated tubular adenocarcinoma in the posterior wall. Both lesions were absolutely independent and isolated. In most instances, multicentric or multiple early gastric carcinomas manifest similar histological features, but in our case, the lesions were of different types.

Adenocarcinoma↗