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Biomedical subjects

T Maruo

Publications and source records attributed to T Maruo.

At least 181 records · Page 10Linked to original sources
PubMed1981

Choriogonadotropin-like antigen in a strain of Streptococcus faecalis and a strain of Staphylococcus simulans: detection, identification, and characterization.

The presence of choriogonadotropin- and alpha-subunit-like materials in two species of bacteria identified as Staphylococcus simulans and Streptococcus faecalis have been demonstrated by the indirect fluorescein-labeled and the indirect peroxidase-labeled immunocytochemical techniques, utilizing antiserum for human choriogonadotropin, for its alpha and beta-subunits and the bera-subunit COOH-terminal peptide. The bacteria were originally isolated from the urine of two patients with advanced forms of cancer. Chromatography done on the water-soluble extract of acetone powder preparations of the bacterial cultures revealed the presence of a material similar to the complete trophoblastic hormone and to its beta-subunit in the culture media of S. simulans, and to the beta-subunit in the media of S. faecalis. No free alpha-subunit was detectable. Furthermore, the choriogonadotropin-like factor demonstrated biological activity in in vivo assay systems. From the present results, it can be concluded that some species of "cancer-associated" bacteria can synthesize a human trophoblastic hormone-like glycoprotein with physicochemical properties similar to those of the human trophoblastic hormone that is biologically active and that is either released complete or as one of its subunits in the culture media.

Chorionic Gonadotropin↗
PubMed1981

[Laboratory and clinical studies on ceftizoxime in otorhinolaryngological field (author's transl)].

From the laboratory and clinical studies on ceftizoxime (CZX), the following results were obtained 1) CZX was compared with cefazolin (CEZ) for in vitro activity against 6 standard strains and clinically isolated strains of Staphylococcus aureus (19 strains), Staphylococcus epidermidis (14), Proteus sp. (17), Escherichia coli (3), Klebsiella sp. (3) and Pseudomonas aeruginosa (13). While somewhat less active against Gram-positive cocci than CEZ, CZX was far more active than CEZ against Gram-negative bacilli. 2) The time course of mean serum CZX levels in 2 patients given a single intravenous dose of 10 mg/kg was as follows: 24.9 microgram/ml at 15 minutes, 18.5 microgram/ml at 30 minutes, 13.2 microgram/ml at 1 hour, 6.4 microgram/ml at 2 hours and 2.7 microgram/ml at 4 hours. The mean serum half life was 1.36 hours. The mean tonsil concentrations of CZX 30 minutes after a single intravenous dose of 0.5 g and 1.0 g were 5.9 microgram/g and 9.6 microgram/g. respectively, with the ratio to the serum concentration of 0.33 and 0.32. 3) CZX was given to 28 patients with ear, nose, and throat infections, and overall rate of effectiveness was 92.3%. No clinical side effects were observed. Changes in laboratory test findings included slightly elevated GOT and/or GPT in 3 cases.

Adolescent↗
PubMed1979

Production of choriogonadotropin-like factor by a microorganism.

Extracts from an acetone powder preparation of a culture of a microorganism tentatively named Progenitor cryptocides contain choriogonadotropin (CG)-like factor as determined by radioimmunoassay with antiserum to human (h)CG beta subunit COOH-terminal peptide and radioreceptor assay with bovine corpus luteum membranes. Possible interference by proteases in the extracts was excluded. Immunoreactive materials reacting with antisera to hCG beta subunit and hCG beta subunit COOH-terminal peptide were also found in the extracts. No free alpha subunit was detected. The CG-like factor was purified by chromatography on Sephadex G-100, concanavalin A-Sepharose, and DEAE-Sephadex A-50. The factor was adsorbed by concanavalin A-Sepharose, suggesting that it contains mannose and glucose moieties. The factor was eluted at the same position as standard hCG on Sephadex G-100. It dissociated into two bands when subjected to sodium dodecyl sulfate/polyacrylamide gel electrophoresis; the bands corresponded to the respective standard hCG subunits. The biological activity of the purified factor as determined by the rat uterine weight assay and the ovarian weight assay was equivalent to 380 (95% confidence limits: 320-490) and 880 (780-1020) international units/mg, respectively. It can be concluded from the present results that a microorganism produces a glycoprotein that is biologically active and has physicochemical properties similar to those of hCG.

Bacteria↗