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Biomedical subjects

T Mano

Publications and source records attributed to T Mano.

At least 271 records · Page 15Linked to original sources

Performance of noncountermovement jump with both knee and hip joints fully extended.

The relationships between neuromuscular performance and biomechanical variables were studied in maximum vertical jumps to examine the factors influencing the performance of a noncountermovement jump. Keeping their knee and hip joint fully extended, five healthy subjects performed four kinds of noncountermovement jumps and one countermovement jump, during which ankle joint angle, platform force, and surface electromyograms of a triceps surae muscle were recorded. In the four noncountermovement jumps, the magnitude of activation and force at the onset of a shortening contraction of the triceps surae muscle were controlled at four different levels. Performance parameters of the noncountermovement jumps, maximum angular velocity of the ankle angle and flight time, correlated with the platform force at the onset of the plantar flexion. Furthermore the integrated electromyograms of the triceps surae muscle before the plantar flexion were correlated with the maximum angular velocity of the ankle angle and the force at the plantar flexion onset. The findings suggest that the efficient utilization of the muscle characteristic contributes to an enhancement of the noncountermovement jump.

Adult↗

Sympathetic nerve activity related to local fatigue sensation during static contraction.

The relationship between autonomic nervous activity and psychophysical responses was studied during static exercise in humans. Muscle sympathetic nerve activity (MSNA) recorded by a direct method of microneurography and the intensity of fatigue sensation in working muscles [levels of fatigue sensation (LFS) scale 0-10] were analyzed in 11 male subjects during static handgrip (SHG). SHG was exerted at a tension of 25% of maximal voluntary contraction until the given tension could no longer be sustained. MSNA, represented as total activity (burst number x mean burst amplitude), and LFS increased in a time-dependent process till the end of the SHG. At the termination of the static exercise MSNA had increased an average of 480% of the resting value. In the simple exponential curve, Y = A expBX, where X was LFS and Y was MSNA. The constants A and B estimated from the total experiments were 84.5 and 0.161, respectively. The correlation between LFS and MSNA was statistically significant. There was a large difference in the value of constant B (0.089-0.278) among the subjects, and a relatively small difference in the value of constant A (37.5-133.8). The increases in both MSNA and LFS during SHG may be mainly related to the same afferent volley from working skeletal muscles. The results indicate that the response of the muscle sympathetic nerve to SHG relates to the psychological feelings of fatigue in the working muscles.

Adult↗

The relative importance of nervous system and hormones to the 2-deoxy-D-glucose-induced hyperglycemia in fed rats.

We examined the relative contributions of hormones and nervous system to the total 2-deoxy-D-glucose (2-DG)-induced central nervous system-mediated hyperglycemia. 2-DG was injected into the third cerebral ventricle in the following four groups of rats, and hepatic venous plasma glucose, immunoreactive glucagon, immunoreactive insulin, epinephrine, and norepinephrine were measured: 1) intact rats; 2) intact rats receiving somatostatin with insulin infusion through the femoral vein to inhibit glucagon secretion and maintain the basal insulin level; 3) bilateral adrenalectomized (ADX) rats to prevent epinephrine secretion; and 4) ADX rats receiving somatostatin with insulin infusion. Comparing areas under glucose curves among the intact rats, those receiving somatostatin with insulin infusion, ADX rats, and ADX rats receiving somatostatin with insulin infusion, the area under the glucose curve was intact rats greater than intact rats receiving somatostatin with insulin infusion greater than ADX rats receiving somatostatin with insulin infusion greater than ADX rats. These results suggest that there are three distinct sympathetic nervous system responses to 2-DG-induced central nervous system-mediated hyperglycemia. 2-DG-induced hyperglycemia is not dependent on only one of those three systems, it is dependent on all of them. The relative potency of the factors to 2-DG-induced hyperglycemia increases in the following order: direct neural innervation of liver (including suppressive epinephrine action on insulin secretion), glucagon, and direct epinephrine action on liver.

Adrenalectomy↗

[Progressive hemifacial atrophy with sympathetic nerve dysfunction of central origin].

A 37-year-old unmarried man was admitted because of gait disturbance and right hemifacial atrophy. Family history was unremarkable. He had an unconscious attack at age 13 and had writer's cramp since age 15. He was thin and lipodystrophic. In reviewing his portraits, hemifacial atrophy was considered to develop in his early teens and to be progressive since then. Pigmented gum, high arched palate, mild mental retardation, pseudo-Argyll Robertson's pupil, sexual impotence, amyotrophy of the left thigh and the right calf, and a limp due to bony abnormalities was detected. Serological tests for syphilis were negative. Bone X-rays disclosed coxa-deformance. Cerebrospinal fluid. EMG, EEG, muscle biopsy and brain CT were normal. Hearing was decreased to 20-35 dB bilaterally. Plasma norepinephrine levels were 450 pg/ml in the supine position and 539 pg/ml in standing. Plasma renin activity was 5.1-5.4 ng/ml/hr. Microneurography revealed highly accentuated muscle and skin sympathetic nerve activities. Hypothermia on the feet, reduced CVR-R and decreased mydriatic response to 5% cocaine instillation were present. Intravenous infusion of norepinephrine and intradermal injection of either acetylcholine or histamine revealed normal results. In the case, sympathicotonia due to dysfunction in the central nervous system is considered to be related to the pathogenesis of hemifacial atrophy.

Adult↗

[Clinical studies of long-term administration of OK-432 in patients with gynecologic cancer].

The clinical value of OK-432 treatments, was evaluated in 215 patients with various types of gynecologic cancer. Five KE OK-432 was administrated by intramuscular injection every other day for up to 5 years, unless severe side-effects were observed. The 5-year survival rate was determined in 51 patients in the OK-432 group and 93 patients in the control group. The survival rate in the OK-432 group was significantly higher in the cervical cancer patients in stages III and IV than in the control group, at from 20 to 29 months and from 37 to 42 months. These results indicated that long period administration of OK-432 might improve the survival rate in patients with gynecologic cancer, especially in those with advanced cervical cancer.

Adult↗

Presence of epidermal growth factor in human tears.

Epidermal growth factor (EGF) is a polypeptide that stimulates the growth of various tissues, including the cornea. The presence of EGF in tears from normal volunteers and in aqueous humor from cataract patients was investigated via human EGF (hEGF)-specific radioimmunoassay. Immunoreactive hEGF was found to be present at similar concentrations in both reflex (ranging from 0.7 to 8.1 ng/ml) and non-reflex tears (ranging from 1.9 to 9.7 ng/ml), but was undetectable in aqueous humor. Immunoreactive EGF in human tears was indistinguishable immunologically, biologically and biochemically from urine EGF and standard hEGF.

Adult↗

[Clinical analyses on moving toes in "painful legs and moving toes"].

Clinical analyses on moving toes were done through two cases of painful legs and moving toes, who had been ill for more than 5 years. Either cases had degenerated lower lumber to upper sacral spines. Clinico-physiological data suggested the presence of polyneuropathy of the sensory type. They showed the lowered skin temperature on the distal portions of lower extremities and acrocyanosis. They, in addition, showed an irregular respiration frequently associated with apnea episodes. Frequency and amplitude of moving toes were increased during Valsalva's maneuver or apnea. Further more, findings were obtained that both the muscle sympathetic nerve activity recorded microneurographically from the tibial nerve and the surface electromyographic activity on the extensor digitorum longs were increased well concomitantly by the occurrence of moving toes. The finding may relevant that moving toes, a type of dyskinesia, may be probably generated by a direct noxious stimulus to the peripheral sensory or autonomic fibers, being strongly influenced by the higher central autonomic (at brain stem?), the extrapyramidal (at corpus striatum?) or some other structures. Moving toes might be a compensatory mechanism to lessen the paucity of blood flow in the lower extremities.

Aged↗

Microneurographic analysis of thyrotropin-releasing hormone effects on sympathetic outflow in human muscle nerves.

In order to clarify the human sympathetic response to thyrotropin-releasing hormone (TRH), microneurographic analysis was performed in 12 healthy subjects. Multi-unit vasoconstrictive impulses of muscle nerve sympathetic activity (MSA) were directly recorded from tibial nerve fascicles together with ECG and blood pressure. Subcutaneous injection of saline (1 ml) had no effect on blood pressure and neurograms of MSA. Following s.c. injection of TRH (0.5 mg, 1 ml), however, a significant increase in heart rate and blood pressure occurred 2 min after injection; the change was statistically correlated with increased MSA, and disappeared after about 10 min. The observation suggests that TRH-activated vasoconstrictive impulses of MSA increase the peripheral vascular resistance and elevate blood pressure. We speculate that TRH directly activates vasomotor centers or higher autonomic centers rather than peripheral organs, because the parallel increase of blood pressure and MSA after TRH injection counteracts the effect of baroreflex mechanism.

Adrenergic Fibers↗

Responses in muscle sympathetic activity to acute hypoxia in humans.

Responses in muscle sympathetic activity (MSA) to acute hypoxia were studied in 13 healthy male subjects under hypobaric hypoxic conditions at a simulated altitude of 4,000, 5,000, and 6,000 m. Efferent postganglionic MSA was recorded directly with a tungsten microelectrode inserted percutaneously into the tibial nerve. Heart rate (HR) and respiratory rate (RR) were counted respectively from the R wave of an electrocardiogram and from the respiratory tracing recorded by the strain-gauge method. The average values of the MSA burst rate and total activity of MSA (burst rate x mean burst amplitude) at 4,000, 5,000, and 6,000 m were 36.4 +/- 2.6, 39.1 +/- 3.1, and 40.2 +/- 4.2 (SE) bursts/min and 616 +/- 138, 794 +/- 190, and 764 +/- 227 arbitrary units, respectively. These values were significantly higher than the values of 27.1 +/- 2.9 bursts/min and 446 +/- 28 at sea level. HR increased significantly at altitudes, but RR did not show significant change. Under severe hypoxic conditions beyond 5,000 m, there were large interindividual differences in the MSA responsiveness to hypoxia. The results indicate that MSA is activated under hypoxia by stimulating the chemoreceptors. However, the central controlling mechanisms that would be affected by hypoxia may also influence the MSA responsiveness under severe hypoxia.

Adult↗

Effect of L-threo-3,4-dihydroxyphenylserine on muscle sympathetic nerve activities in Shy-Drager syndrome.

We observed changes in postganglionic efferent discharges of muscle sympathetic nerve (muscle sympathetic activity, MSA) microneurographically before and after the oral administration of L-threo-3,4-dihydroxyphenylserine (L-threo-DOPS), a precursor of norepinephrine, in a patient with Shy-Drager syndrome and irregular fluctuations of blood pressure. Before drug administration, MSA was only rarely observed with the patient in the supine position. There was a slight increase in MSA during head-up tilting to 40 degrees, and orthostatic hypotension (OH) occurred just after the body was tilted head upward to 40 degrees. MSA became prominent 30 minutes after the oral administration of 200 mg of L-threo-DOPS while the patient was in a 40 degree head-up position, and the OH was improved. The MSA discharge rate decreased and OH reappeared 3 hours after oral administration, when the plasma concentration of norepinephrine was at its highest level. We suggest that the OH improved mainly because of the increase in MSA due to L-threo-DOPS, and that the drug may activate sympathetic outflow at a site proximal to the sympathetic ganglion.

Autonomic Nervous System Diseases↗

[Screening for endometrial cancer using a curettage stamp method].

The present study shows the value of a routine screening program for the detection of endometrial malignancies. From 1980 to 1985, 5,640 subjects were screened for endometrial malignancies using the curettage stamp method. Of 60 cases whose smears were abnormal, 25 were found to have malignant lesions. These patients included endometrial cancer (15 cases), sarcoma (3 cases) and metastatic cancer (4 cases). Three cases were found to have cervical cancer. Among 22 patients, excluding 3 cases with cervical cancer, 17 cases had negative results in a cervical smear test. Also 3 cases of endometrial cancer showed no history of abnormal genital bleeding. All 15 patients with endometrial cancer were over 40 years of age. These results indicated the clinical importance of the routine endometrial cytological smear in the detection of malignancies of the uterine body, especially in women over 40 years of age.

Adult↗

[Treatment of gynecological cancer with cis-platinum].

Seven patients with gynecological cancer were treated with cis-platinum. Cis-platinum at 50 mg/m2 was administered by intravenous infusion for 2 hours, followed by 2,000 ml of intravenous fluid. This treatment was repeated every three weeks. Five of the seven patients were evaluable. These patients included those with ovarian cancer (3 cases), metastatic ovarian cancer (1 case), and endometrial cancer (1 case). One patient attained a complete response, two a partial response, and two showed progressive disease. The response rate for both complete and partial response was 60%. Gastrointestinal symptoms were most frequently found. Nephrotoxicity was generally mild, but one patient had to discontinue cis-platinum treatment due to severe nephrotoxicity. Cis-platinum treatment is considered to be effective and useful for gynecological cancer. Second-look operation and two-channel chemotherapy were also discussed in this paper.

Adenocarcinoma↗

Responses in muscle sympathetic nerve activity to sustained hand-grips of different tensions in humans.

To clarify whether sympathetic nerve activity increases in relation to the tension of a sustained muscle contraction, muscle sympathetic nerve activity (MSA) was recorded directly from the peroneal nerve fascicle at the popliteal fossa by means of tungsten microelectrodes in five healthy male subjects. A sustained muscle contraction was performed by handgrip for two minutes in a supine position at tensions of 10, 30 and 45% of maximal grip strength (MGS). MSA, electrocardiogram (ECG) using bipolar electrodes from the chest and surface electromyogram (EMG) from the extensor pollicis longus were recorded simultaneously before and during the sustained handgrip. Arterial blood pressure was measured at the resting upper arm by auscultation. During handgrip with tensions of 10, 30 and 45% MGS, average MSA burst rate (bursts X min-1) increased to 122, 152 and 230% of the resting value, respectively. During the same experimental procedures with tensions of 10, 30 and 45% MGS, average heart rate increased to 105, 110 and 111% of the resting value. These results confirm that sympathetic outflow to a resting muscle is increased with elevation of tension in an active muscle. This process would promote perfusion pressure in the active muscle.

Adult↗