Search PubMed⌕ Search

Biomedical subjects

T Manabe

Publications and source records attributed to T Manabe.

At least 523 records · Page 29Linked to original sources
PubMed1982

A normalized protein map of human plasma, and characterization of the normalized spots by microscale amino acid analysis.

A "normalized map" of human plasma proteins, which shows the standard distribution pattern of the proteins separated two-dimensionally in the absence of denaturing agents, is described. The pI and molecular mass of the protein represented by each spot in the normalized map are shown, and 84 of 128 spots are identified or tentatively identified as known plasma proteins. Of the several methods we used for the identification, the method of micro-scale amino acid analysis of the proteins extracted from the fixed, stained two-dimensional gels is described in detail. Patterns for human cerebrospinal fluid and urinary proteins are compared with the normalized map, the positions of proteins characteristic of each body fluid sample being shown.

Amino Acids↗
PubMed1982

Microscale multisample two-dimensional electrophoresis of proteins in human serum, cerebrospinal fluid, and urine.

In this technique, in which no denaturing agent is used, proteins in human serum, cerebrospinal fluid, and urine are separated by isoelectric focusing in cylindrical 40 g/L polyacrylamide gels of capillary size (1.3 x 35 mm) for 40 min, followed by electrophoresis in 40--170 g/L polyacrylamide linear gradient gel, with use of 38 x 35 x 1 mm slab gel, for 1 h. Only 2 microL of untreated human serum is required to obtain clear protein-distribution patterns, made visible by Coomassie Blue staining. By use of silver staining, proteins in unconcentrated cerebrospinal fluid can be made visible. An apparatus we devised for microscale two-dimensional electrophoresis enables us to analyze eight protein samples simultaneously.

Blood Proteins↗
PubMed1981

Detection of the changes in protein distribution of rat plasma induced by carbon tetrachloride administration by means of two-dimensional electrophoresis.

The changes in rat plasma protein distribution after carbon tetrachloride administration were examined using two-dimensional electrophoresis, utilizing isoelectric focusing in polyacrylamide gel in the first dimension and pore gradient polyacrylamide gel electrophoresis in the second dimension. Drastic changes in amount of protein were observed at more than 20 spot positions including those of transferrin, Gc-globulin and low-density lipoprotein. The time course of the changes was examined, and the most drastic changes were observed at 2 days after carbon tetrachloride administration.

Animals↗
PubMed1981

Normalization of two-dimensional electrophoretic patterns of human plasma proteins and comparisons of cerebrospinal fluid and urine patterns in terms of the normalized map.

A "normalized map" of human plasma proteins, which illustrated the standard distribution pattern of the proteins, was prepared by comparing eight two-dimensional electrophoretic gels of normal human plasma. Phenotypes of several plasma proteins were detected. The pI and molecular weight of each spot in the normalized map were calculated and 84 spots out of 128 were identified or tentatively identified as known plasma proteins by several methods including micro amino acid analysis of the proteins extracted from the stained gel pieces. Human cerebrospinal fluid and urine protein patterns were compared with the normalized map and the positions of proteins characteristic of each body fluid sample were determined.

Blood Protein Electrophoresis↗
PubMed1981

Preparation and characterization of cobalt(II)-substituted bovine serum amine oxidase.

The Type-2 copper ion in bovine serum amine oxidase (BSAO) was first replaced by cobalt(II) ion. The enzymatic activity of Co(II)BSAO was 13.3% of that of native BSAO. The various spectral data indicated that the Co(II) center has tetrahedral geometry (high-spin state) and is linked by two nitrogens and two oxygens. It was also found that the putative organic chromophore suggested by many investigators exhibits a positive CD band near 370 nm and a negative CD band near 440 nm.

Animals↗
PubMed1981

Ultrastructural observation of neonatal lungs with transmission electron microscope and freeze-fracture replication.

Nine neonatal and one child lungs in various conditions were examined with electron microscopy combined with thin section and freeze-fracture replication techniques in order to study mainly ultrastructural changes of lamellar inclusion bodies of type II alveolar cells. Lungs from neonates with respiratory distress syndrome and with bronchopneumonia showed occasional lamellar inclusions that were partially amorphous in thin section and granular and fragmentarily linear in freeze-fracture preparation. This change was considered to represent focal micelle formation of phospholipid lamellae. No specific morphological changes were noted in lungs of respiratory distress syndrome. In contrast, lungs from thanatophoric dwarfism showed ripple appearance of inclusion lamellae. This was considered to possibly represent unusual phospholipid compounds forming lamellae. Other interesting findings were also described.

Child, Preschool↗
PubMed1981

Mutagenicity studies on alcohol extracts from gamma-irradiated potatoes--preparation of samples and their chemical analysis.

The preparation of alcohol extracts from gamma-irradiated potatoes of the "Danshaku" variety and their chemical aspects were studied. The final concentrate of alcohol extracts from potatoes showed pH values of 3-4. The o-quinones or "radiotoxins" reported by Kuzin, et al. were not detected in the alcohol extracts from potatoes of this variety by high performance liquid chromatography (HPLC) analysis, paper chromatography and the model enzymatic experiment.

Alcohols↗
PubMed1981

Manifestations of carcinoma of the uncinate process by means of superior mesenteric arteriography.

Identification of carcinoma arising in the uncinate region of the pancreas is difficult because of the peculiar topographic relationship of this lesion with adjacent structures. Among the various diagnostic procedures performed upon ten patients with this malignant lesion, superior mesenteric arteriography provided the most helpful information, including signs of invasion in the main trunk of the superior mesenteric artery and vein and in the proximal portion of the jejunal arteries. A clearer visualization of these vascular involvements was attained in the right posterior oblique projection rather than in the anteroposterior projection. On the contrary, the arterial and portal vessels belonging to the celiac territory, such as superior pancreaticoduodenal arteries, dorsal pancreatic artery, gastroduodenal artery, splenic vein and portal vein, were almost free of involvement. At laparotomy, almost all of the patients had direct extension of the tumor to the superior mesenteric vessels, forming a cancerous core in the root of mesentery. All died of cachexia within six months after a palliative surgical procedure. Serial roentgenograms of superior mesenteric artery, especially taken in the oblique projection, are the best means of confirming carcinoma of the uncinate process, for which only a few available methods have been established to evaluate the clinical aspects.

Adenocarcinoma↗
PubMed1981

Oxidation of epsilon-amino group of lysyl peptides by bovine serum amine oxidase.

We examined whether bovine serum amine oxidase (BSAO) was able to oxidize lysyl peptides. Seven synthetic peptides, Z-Lys-Leu-OMe, Z-His-Lys-Leu-OMe, Z-Val-Leu-Gly-Lys-Leu-OMe, Ala-Ala-Lys, Ala-Lys-Ala, Phe-Lys, and Gly-Lys were incubated with BSAO at 37 degrees C for 4 days, and the extent of oxidation (H2O2 formation) was assayed by o-dianisidine method. The amino acid sequence of lysyl peptides affected the oxidation rate by BSAO. The rate of oxidation of Z-Lys-Leu-OMe was about fifty fold higher than that of Z-His-Lys-Leu-OMe. Z-Lys-Leu-OMe, the lysyl peptide most reactive with BSAO was analyzed by the 3-methyl-2-benzothiazolinone hydrochloride method, amino acid analysis, and the 2,4,6-trinitrobenzene sulfonic acid method. The production of aldehyde, a decrease in the lysine/leucine ratio, and a decrease in epsilon-amino group were confirmed. These results show that bovine serum amine oxidase is able to oxidize the epsilon-amino group of lysyl peptides.

Aldehydes↗
PubMed1980

Two-dimensional electrophoresis of immunoglobulin myeloma proteins in the absence of denaturing agents.

Human sera from myeloma patients were subjected to two-dimensional electrophoresis. Heterogeneity in molecular weight and in isoelectric point of the myeloma proteins were demonstrated on the protein map. The patterns of five major immunoglobulin classes differed from each other, implying that the five classes of myeloma immu,oglobulin can be distinguished by examining the two-dimensional electrophoretic patterns. Sugar content analysis suggested that differences in sialic acid content may be one of the origins of the heterogeneity of myeloma proteins observed in isoelectric focusing.

Amino Acids↗
PubMed1980

Angiography and pancreatoductography in resectable carcinoma of the pancreas.

Angiography and pancreatoductography were performed in 18 patients with resectable carcinoma of the pancreas. The presence or absence of vascular and ductal tumor involvement was evaluated. Accurate diagnosis was obtained in 83 per cent at pancreatoductography alone, in 66 per cent at angiography alone, while combination of the methods resulted in 94 per cent accuracy. Tumor involvement of the capsule and secretion stagnation in the distal part of the pancreas are of importance for the surgical management and the survival after resection. An improved result after surgery may be obtained by combined evaluation of the vascular and ductal morphology as demonstrated at preoperative radiography.

Celiac Artery↗
PubMed1980

Protective effects of PGE2 on diet-induced acute pancreatitis in mice.

Prostaglandins have been noted to have a "protective" effect against gastrointestinal mucosal injury induced by a wide variety of agents although possible protective effects of prostaglandins on injury to other tissues have not been reported. We have tested the effect of prostaglandin E2 (PGE2) on acute experimental pancreatitis induced by feeding young female mice a choline-deficient ethionine-supplemented (CDE) diet for 24 hr. Administration of 0.05--0.20 microgram PGE2/g body wt 1 hr before and 4 hr after institution of the CDE diet lowered the mortality rate of diet-induced pancreatitis from 56% to 31%. Larger and smaller doses of PGE2 were without effect. Administration of PGE2 (0.10 microgram/g body weight) diminished the rise in in-vitro LDH discharge and the increase in "free" Cathepsin D activity which occur during diet-induced pancreatitis. Similarly, PGE2 (0.10 microgram/g body wt) diminished the magnitude of the increase in in-vitro protein discharge and the elevated concentrations of trypsinogen and chymotrypsinogen in pancreas fragments taken from mice given the CDE diet. These findings indicate the PGE2 has a protective effect against CDE diet-induced acute experimental pancreatitis. The Cathespin D and LDH changes noted during CDE diet-induced pancreatitis suggest that this diet may decrease membrane integrity and thus allow these enzymes to leak out of the lysosomes and acinar cell, respectively, during pancreatitis. Although the basis for the protective effect of PGE2 remains unclear, our observations suggest that the prostaglandin may act to reduce the alteration in membrane integrity which occurs during CDE-diet induced pancreatitis.

Amylases↗