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Biomedical subjects

T Kurihara

Publications and source records attributed to T Kurihara.

At least 343 records · Page 19Linked to original sources
PubMed1983

Cloning of the nusA gene of Escherichia coli.

The EcoRI restriction fragment of wild-type Escherichia coli DNA that can complement the argG mutation was cloned into the EcoRI site on pBR322. The resulting chimeric plasmid (pYN81) contains a 16.0 kilobase fragment and can complement the nusA1 (Friedman 1971) as well as argG mutations. Examination of several deletion derivatives of pYN81 revealed that the activity that complements nusA1 and argG mutations is localized within the 5.3 kilobase segment defined by SalI and BglII sites. When the nusA gene segment was recloned into lambda vector L512, the resulting transducing phages lambda nusA+-1 and lambda nusA+-2 grew normally in nusA1 cells at 40 degrees C or above, unlike the vector phage L512. Proteins encoded by the cloned DNA fragment were examined with minicells containing the chimeric plasmid or UV-irradiated cells infected by the transducing phages. At least six proteins were apparently encoded by the 16.0 kilobase DNA fragment, and genes coding for each of these proteins were localized on the respective restriction segment. One of them with a molecular weight of 64,000 was identified as the nusA gene product. The nusA gene was found to be transcribed counterclockwise with respect to the E. coli genetic map. Endonulease BglII cleaves the gene in the vicinity of the C-terminus, generating a truncated nusA gene product with a molecular weight of 61,000 that can complement the nusA1 mutation.

Argininosuccinate Synthase↗
PubMed1983

[Antibacterial peptides (18). Synthesis and antibacterial activity of alpha-acyltetrapeptides containing basic amino acids (7)].

Three kinds of (formula; see text) were synthesized to investigate the relationship between the chemical structure and the antibacterial activities by acylation of the corresponding tetrapeptides. Acyl chlorides of (+)-6-Methyloctanoic acid (a component of colistin) and two kinds of other organic acid were employed as acylating agents. It was proved that one of three, (formula; see text) shows antibacterial activity against four kinds of Gram-negative bacteria and against Gram-positive bacteria, but that the other only have weak antibacterial activity.

Chemical Phenomena↗
PubMed1982

Hydration and percutaneous absorption III: Influences of stripping and scalding on hydration alteration of the permeability of hairless mouse skin to water and n-alkanols.

The influence of hydration on the permeability of stripped and scalded skins of hairless mice was investigated in vitro using water and n-alkanols as test permeants. Irrespective of pretreatment, the permeation rates of water, methanol, and ethanol were unaffected by aqueous immersion of skin sections in a diffusion cell, consistent with earlier data on unprocessed skins. The permeation rates of butanol and hexanol also were insensitive to hydration, differing from earlier studies on normal, intact skin in which both solutes' rates doubled after 10 hr of soaking. Following both pretreatments, the permeability of octanol declined over the first 5-10 hr of maceration, but remained invariant thereafter. The decline was most pronounced for the scalded skins. With untreated skin, octanol permeability initially increased and then declined before assuming a constant value. This study indicates that the barrier properties of the epidermis and dermis are not particularly sensitive to extended hydration except in the case of octanol. Scalding at 60 degrees for 60 sec rapidly hydrates the skin, altering tissue permeability to about the same extent as a 10-hr (or longer) immersion in water at 37 degrees. Octanol's unique hydration profile is explained by locating the origin of permeability decline in tissue beneath the horny exterior of the skin.

Abdomen↗
PubMed1982

Hypokalemic myopathy associated with 17 alpha-hydroxylase deficiency: a case report.

We studied a patient with hypokalemic myopathy associated with 17 alpha-hydroxylase deficiency. An 18-year-old high school student, who appeared to be a girl with poorly developed secondary sex characteristics, had generalized muscle weakness. The cause of muscle weakness proved to be hypokalemic myopathy confirmed by clinical findings and muscle biopsy. Endocrinologic study demonstrated 17 alpha-hydroxylase deficiency with male pseudohermaphroditism. The metabolic abnormality of this patient was corrected by the administration of glucocorticoid. The possibility of this rare disease has to be considered when we examine a patient who has hypokalemic myopathy associated with hypogonadism.

Adolescent↗