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Biomedical subjects

T Kurata

Publications and source records attributed to T Kurata.

At least 379 records · Page 21Linked to original sources
PubMed1978

[Inhibition of tumor growth and metastases in mice with Lewis lung tumors by vitamin A and BCG].

The anti-tumor effect of vitamin A and/or BCG was investigated in Lewis lung tumor system. Tumor growth and lung metastases were significantly suppressed, when tumor cells were mixed with BCG and inoculated subcutaneously into vitamin A-treated animals. Survival time was also prolonged by the same treatment. Vitamin A alone, without BCG, showed no effect on tumor growth, lung metastases or survival time.

Animals↗
PubMed1978

Correlation of immune response with clinical stage in Lewis lung tumor-bearing mice.

The effect of Lewis lung tumor growth in mice on the induction of primary immune response to SRBC, was investigated by PFC assay for measuring antibody activity and by footpad test as a correlate for delayed type hypersensitivity reactions. With the appearance of micrometastases in the lungs there was a decline in the humoral and cellular immune response to the SRBC. An increase of number and size of metastases in the lungs led to a further depression of the immune reactivity. Since the reduction of general immune response in mice bearing this tumor is not due to a direct influence of tumor cells, it might be assumed that suppressor cells or factors, are actively abrogating the general and also the tumor directed immune reactions.

Animals↗
PubMed1978

The microsurgical revascularisation of resected segments of tibia in the dog.

A segment of tibia 4.5 centimetres long was removed from one hind limb of fifteen dogs. It was then replaced and the main vasculature was restored by a microsurgical technique. In eight controls the segment was replaced without such restoration. In two-thirds of the former cases the microvascular reconstruction was successful; the rate of infection was found to be reduced, bone union was guaranteed and the rate of union accelerated. Success or failure of the reconstruction was clearly demonstrated in five cases by early bone scanning using technetium-labelled polyphosphate.

Animals↗
PubMed1978

Reactivation of herpes simplex virus (type 2) infection in trigeminal ganglia and oral lips with cyclophosphamide treatment.

Age-dependent resistance in trigeminal nerve against type 2 herpes simplex virus infection through oral lips and reactivation of the latent virus infection with cyclophosphamide in the ganglion in correlation to theprimary infected part were studied in mice with immunofluorescent and histopathological methods. The mice acquired resistance in the nervous tissue after 8 weeks of age. The viral antigen appeared in all trigeminal ganglia immediately after infection, but not found at 4 weeks later. Reactivation of the latent infection was tried by using 8-week-old mice with cyclophosphamide treatment. Four to five times administration of the drug produced the leasions of herpes simplex virus infection in the ganglia and also in the primary infected areas, but viral antigen-bearing neurons in the ganglia were very few.

Age Factors↗
PubMed1978

Vertical transmission of type 2 herpes simplex virus infection in immunosuppressed hamsters.

The effect of immunosuppressive procedure with cyclophosphamide on the vertical transmission of the type 2 herpes simplex virus in hamsters was investigated. Single injection of the cyclophoshamide one day before virus inoculation in various stages of pregnant course caused high rate of placental and fetal infection of the virus. Immunofluorescence technique revealed the presence of viral antigen in more than 90% of placentas and 29% of fetuses with treatment at the middle of late stage of gestation. The drug treatment alone without viral infection brought no detectable changes in placentas or fetuses, whereas slight depletion of mononuclear cells in perifollicular area of the spleen was found. The drug could destroy easily the defense mechanism of the placenta.

Animals↗
PubMed1977

Herpes simplex encephalitis: an autopsy case with isolation of type 1 herpes simplex virus.

An adult case of herpes simplex encephalitis was studied after autopsy. Postmortem examination revealed necrotizing encephalitis associated with Cowdry type A intranuclear inclusion bodies in glial cells. Herper simplex virus type 1 was isolated from the removed brain. Herpes simplex virus antigens were detected diffusely in wide areas of the brain by immunofluorescent test and viral particles characteristic to herpes simplex virus were demonstrated by electron microscopy. There was an apparent discrepancy between severity of histological changes and distribution of virus antigen.

Adult↗
PubMed1977

Experimental micro-anastomosis of 0.4-0.5 mm vessels.

Ninety-two anastomoses of arteries 0.5 mm in diameter have been performed in rats with an 85-90 per cent patency rate in the second postoperative week. The factors of prime importance in the achievement of high patency rates have been atraumatic handing and meticulous suturing technique. At 1 hour and at 8 to 10 days the patency rates were not statistically different whether 20 per cent magnesium sulphate heparin or normal saline were used. Similar results were obtained with 40 repairs of divided veins averaging 0.4 mm in diameter. These results suggest strongly that clinical microvascular surgery in vessels approximately 0.5 mm in diameter, especially in children, should given results comparable to 1 mm vessel surgery.

Animals↗
PubMed1977

Lewis lung tumor system as a model for studying the immune function in syngeneic in equillibrium allogeneic chimeras.

Lewis lung tumor (LLT) passaged in F1 hybrids of the original C57B1/6 strain, where it arose spontaneously, is rejected by allogeneic SWA (Swiss albino) mice. However, aggregation chimeras derived from these SWA mice and the F1 hybrids of C57B1/6 developed an increased growth of primary tumor and reduced number of metastases when compared with the F1 hybrids. In the present study LLT passaged in C57B1/6 mice is not rejected by SWA mice. It is demonstrated that in aggregation chimeras now derived from two strains both taking the tumor, primary tumor growth was enhanced and the number of metastases reduced as in the former experiment. The tendency of reactions of lymphatic orgains in chimeras to tumor burden was comparable with the SWA and F1 hybrid (C57B1/6 multiplied by SWA and SWA multiplied by C57B1/6) recipients' response. Furthermore, chimeras had the highest spleen enlargement. Possible alternations in immune functions of chimeras due to their differing genotype combination are discussed in the LLT model.

Animals↗
PubMed1977

Suppressed tumor growth and metastasis by vitamin A + BCG in Lewis lung tumor bearing mice.

The anti-tumor effect of vitamin A and/or BCG was investigated in Lewis lung tumor system. Tumor growth and lung metastases were significantly suppressed, when tumor cells were mixed with BCG and inoculated subcutaneously into vitamin A-treated animals. Survival time was also prolonged by the same treatment. Vitamin A alone, without BCG, showed no effect on tumour growth, lung metastases or survival time.

Animals↗
PubMed1977

Demonstration of vaccinia virus antigen in brains of postvaccinal encephalitis cases.

Five lethal cases of postovaccinal encephalitis (PVE) were examined in attempts to detect the viral antigen by the fluorescent antibody techniques and to isolate the infectious vaccinia virus. Histologically, the brain tissues were characterized by meningoencephalitis, with perivascular infiltrations and inflammatous reactions mainly in leptomininx, chroid plexus and ependyma. By direct immunofluorescence staining, vaccinia virus antigen was found in the brain specimens of one case, but not in the other four cases. The existence of antigen-antibody complexes was suggested by the deposition of immunoglobulins in the brain specimens. After treatment with 3 M NaSCN solution to dissociate the immunoglobulins from the complex, the viral antigen was demonstrated in specimens of two cases. Localization of viral antigen(s) thus detected coincided with that of meningoencephalitic reactions in the histological examination. Virus isolation on chick embryo chorioallantoic membrane was negative in all cases. Participation of viral pathogenicity in the etiology of PVE is discussed.

Antigen-Antibody Complex↗
PubMed1977

Pathogenicity of a vaccine strain of vaccina virus in rabbits administered by intravenous route.

The pathogenicity of the Lister (Elstree) strain, a vaccinia virus, in rabbits after intravenous injection was studied by histopathological and immunofluorescent methods. Inoculation of 1 X 10(7) PFU (pock-forming unit) virus into weanling and young adult rabbits caused severe emaciation and high mortality within 2 weeks. Pathological findings were characterized by vesicular lesions along muco-cutaneous junction areas of the eyes, nose and mouth and by inflammatory changes in the brain, mainly in the meninges and choroid plexus. Immunofluorescent staining of the tissues of animals sacrificed at intervals demonstrated the accumulation of vaccinia viral antigen (s) in the loci of pathological changes. The suitability of this model system for the study of pathogenesis of human postvaccinal meningoencephalitis is discussed.

Animals↗
PubMed1976

Enhanced tumor growth in chimeric mice.

Chimeras were produced by aggregation of B6D2F1 and SWA early embryos. Lewis lung tumor, syngeneic in B6D2F1, was used to study tumor growth and metastases in these chimeras. Enhanced tumor growth, low metastases rate and pronounced enlarged spleen could be observed in SWA equilibrium B6D2F1 chimeras 21 days after inoculation of tumor cells. Increased activity of suppressor T-cells which might be due to permanent allogeneic stimulation in chimeras is discussed as one of the possible mechanisms.

Animals↗
PubMed1976

Vertical transmission of type I and type 2 Herpes simplex virus infection in hamsters.

The relation of type 2 herpes simplex virus infection during the several stages of embryogenesis of hamster was examined using histopathological and immunofluorescent techniques. The comparative investigation between type 2 and type 1 was done. Both types of herpes simplex virus caused placental lesions and vertical transmission in fetuses when the virus was inoculated intravenously into pregnant hamsters at the middle and late stage of gestation.

Animals↗