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Biomedical subjects

T Kurata

Publications and source records attributed to T Kurata.

At least 361 records · Page 20Linked to original sources
PubMed1982

Measurement of functional residual capacity and pulmonary carbon monoxide diffusing capacity during mechanical ventilation with PEEP.

First, our new simplified method to measure FRC and DLCO simultaneously during mechanical ventilation was described in detail. Secondly, we applied the method to ARDS patients and observed the effects of PEEP on arterial blood gases (ABGs), FRC and DLCO of these patients. As reported hitherto, FRC was consistently increased by PEEP, whereas ABGs in some cases were not necessarily improved with increase in FRC. DLCO/FRC remained unchanged, although DLCO increased with PEEP. We concluded that the dissociation of FRC and ABG data in a group of patients could be caused by wasted ventilation which might be attributed to VA/Q unevenness.

Adult↗
PubMed1982

Effects of inspiratory pressure oscillation on pulmonary gas exchange and circulatory functions in anesthetized, mechanically ventilated dogs.

Anesthetized, mechanically ventilated dogs were used to study the effects of inspiratory pressure oscillation on gas exchange. Respiratory failure was induced in dogs artificially, changing either tidal volume or ventilatory rate. Pressure oscillation of approximately 2 Hz with amplitudes of 2 to 7 cmH2O was applied on the inspiratory phase of mechanical ventilation. PaO2 and PaCO2 were improved consistently by pressure oscillation while minute ventilation was kept fixed. No significant change was observed in AaDO2, cardiac output, heart rate, ECG and both systemic and pulmonary arterial pressures under oscillated ventilation. The oscillated ventilation could be continued for more than 60 minutes without causing any significant change in circulatory function. The ratio of alveolar ventilation to minute ventilation (VA/VE) increased and that of dead space to tidal volume (VD/VT) decreased significantly. The oscillated ventilation while breathing He-O2 and SF6-O2 showed no consistent difference in the effects on gas exchange. It can be concluded that simple pressure oscillation improves gas mixing in the lungs and may be applied to respiratory care. However the mechanism remains to be elucidated.

Animals↗
PubMed1982

[Clinical study of preoperative chemotherapy of primary breast cancer. 1. Efficacy of a combined use of CPA (endoxan) and FT-207 (or 5-FU dry syrup)].

In twenty-nine cases of primary breast cancer preoperative treatment using CPA and FT-207 (or 5-FUDS) was performed to determine their efficacy. Daily dose of each anticancer drugs was as follows: CPA 50-200 mg, FT-207 200-600 mg, 5-FUDS 200 mg, p.o.. The total doses were CPA 1.8 g, FT-207 5.0g, 5-FUDS 3.4 g in average. In 11 cases (37.9%) reduction in tumor size was obtained. According to Ohboshi's criteria, over Grade II a effect was seen in 5 cases (17.2%), while Grade III effect was not seen in any of the cases. Effective cases were more frequently observed among those which received CPA at 25 mg/kg or more, or FT-207 (5-FUDS) at 80 mg/kg or more. Main side effects were G. I tract symptoms such as anorexia and nausea. To obtain definitive conclusion on the clinical significance of use of CPA and FT-207 (5-FUDS) as preoperative chemotherapy for breast cancer, further studies are required.

Adult↗
PubMed1982

Paraquat poisoning--a review of four cases.

Paraquat poisoning has been associated with very high mortality. The toxic mechanism is considered to be related to the production of superoxide ions. Review of the literatures reveals that with vigorous treatment recovery from poisoning is not impossible. Clinical experience in four cases of paraquat poisoning and the principles of management of this intoxication are presented.

Adult↗
PubMed1981

Loss of membrane excitability after herpes simplex virus infection in tissue-cultured nerve cells from adult mammals.

Dorsal root ganglion cells of adult guinea-pigs in vitro were inoculated with herpes simplex virus (HSV). Intracellular recording with a glass microelectrode revealed that HSV-infection caused a marked decrease in membrane excitability of the nerve cells within 24 h, which could be explained as a decrease in Na+ channel activity in the plasma membrane; while changes in other physiological properties (resting membrane potential, membrane resistance and capacitance) remain small. Viral antigens were detected in only 10-15% of the HSV-infected nerve cells. These indicate that the loss of the membrane excitability occurs much earlier than any other change in the HSV-infected nerve cells.

Animals↗
PubMed1981

Separative determination of ascorbic acid and erythorbic acid by high-performance liquid chromatography.

High-performance liquid chromatography (HPLC) was applied to the determination of ascorbic acid (AsA) and erythorbic acid (ErA). The apparatus was a Shimadzu model LC-2P Liquid Chromatograph equipped with a UV detector set at 254 nm. The separation was achieved on a LiChrosorb-NH2 column which was pre-treated with 0.1 M ammonium monophosphate solution using a mixture of acetonitrile, acetic acid and water (87:2:11, v/v) as an eluant. The HPLC method has the following advantages: AsA and ErA are quantitated after being distinctly separated, analysis time per one sample is short, and AsA or ErA levels as low as 1.0 X 10(-2) microgram are detectable. Recovery experiments with dehydro-AsA and dehydro-ErA, involving reduction with H2S, give satisfactory results.

Antioxidants↗
PubMed1981

Separative determination of ascorbic acid and erythorbic acid in animal tissues by high-performance liquid chromatography.

A method for the separation and determination of ascorbic acid (AsA) and erythorbic acid (ErA) in animal tissues is described. It employs high-performance liquid chromatography on a LiChrosorb-NH2 column in conjunction with a mixture of acetonitrile, acetic acid and water as an eluant. Application of the method, which is sensitive, rapid and simple, to the analyses of AsA and ErA in animal tissues such as liver, adrenals, spleen, kidneys and heart gave satisfactory results. Dehydro-AsA and dehydro-ErA in rat liver could be determined after reduction with H2S. The method was shown to be useful for the routine analyses.

Adrenal Glands↗
PubMed1981

Consistent appearance of microtubules in cells productively infected with various strains of type 2 herpes simplex virus.

Electron microscopic examination showed microtubular structures in FL cells infected with all 18 strains of HSV-2 examined, but not in cells infected with 9 strains of HSV-1. These structures were also detected in other cultured cells (Vero and Earle's L cells) infected with HSV-2, and also in vivo in cells, such as neuronal cells of the spinal ganglia and liver cells, of one-day-old suckling mice (DDD strain) infected with this type of virus. Thus the microtubules were consistently detected in cells productively infected with HSV-2. No other herpesviruses so far examined produced microtubular structures such as those observed in HSV-2 infected cells.

Animals↗
PubMed1980

Analysis of the inhibitory effect of peritoneal macrophages on the spread of herpes simplex virus.

Peritoneal macrophages obtained from mice after an intraperitoneal injection of tryptose peptone inhibited the development of herpes simplex virus type 2 plaques in syngeneic mouse embryonic fibroblasts. In contrast, peritoneal macrophages, spleen cells, and thymocytes from untreated mice showed only a minimal inhibitory effect on the development of viral plaques. The effect was age dependent. Macrophages from 2 and 3-week-old mice showed weaker functions, requiring a larger number of cells for an equivalent reduction of plaques and virus yield than those from adult mice. When macrophages were treated with procaine, their phagocytic activity was completely abolished. However the procaine-treated macrophages still could inhibit the development of viral plaques. Peritoneal macrophages did not show any increased cytotoxicity against herpes simplex virus-infected cells; plaque inhibition might rather be attributable to their cytostatic effects on target cells.

Aging↗
PubMed1979

Widespread eczema vaccinatum acquired by contacts. A report of an autopsy case.

A 4-month-old male infant predisposed to allergic dermatitis acquired wide-spread eczema vaccinatum by contacts with a recently vaccinated sibling. He died of acute purulent peritonitis following a perforation of multiple duodenal ulcers. Fluorescence immunocytochemical and electron microscopic studies on the skin lesions revealed the presence of viral antigens and numerous virus particles compatible morphologically with those of the mature form from the same batch of smallpox vaccine given to the sibling. A large number of virus particles in the developmental form were also predominantly scattered in the cytoplasm of cells at the stratum malpighii of the epidermis as well as in neutrophils and macrophages in the skin lesions. The virus isolation from the skin lesions was done by using the HeLa cells and the human embryonic lung fibroblasts. No abnormal laboratory data were noted in immunoglobulins. On the basis of atrophy of the thymus and other lymphatic tissues and an appearance of large pyroninophilic cells in association with blastoid transformation, the authors discussed a possible participation of the disturbance of cellular immunity secondary to the virus infection in the development of the disease.

Antigens, Viral↗
PubMed1978

Microvascular surgery in degloving injuries: an experimental study.

Using an experimental model in the rabbit, the importance of direct cutaneous vessels for skin flap survival in skin avulsion injuries with, and without, a crushing injury has been investigated. The results are presented and possible application to the human situation discussed.

Animals↗