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Biomedical subjects

T Kitamura

Publications and source records attributed to T Kitamura.

At least 811 records · Page 45Linked to original sources
PubMed1980

Colonization and pathogenicity of Saccharomyces cerevisiae, MC16, in mice and cynomolgus monkeys after oral and intravenous administration.

Saccharomyces cerevisiae, an ascomycetous yeast and a candidate for a host-vector system in recombinant DNA experiments, was examined for its pathogenicity and colonization in mice and cynomolgus monkeys as the models of the biological containment level. Adult mice given perorally with 5.5 or 2.4X10(7) cells of MC16 strain S. cerevisiae excluded them rapidly and no colonization of the cells in the abdominal organs, lymph nodes or gastrointestinal wall was demonstrated. No change in the fecal flora was observed. Cynomolgus monkeys after peroral administration of 4.9X10(7) or 7.8X10(8) cells showed a similar tendency of rapid exclusion and the lack of colonization. Cortisone acetate treatment had no significant effect. Intravenous administration of 3.9X10(7) yeast cells had no pathogenic effect and no viable yeast cell was detected in the blood. The biological containment level of S. cerevisiae was suggested to be not lower than that of E. coli K12 B1 level, and the possibility of achieving the B2 level was also suggested.

Administration, Oral↗
PubMed1980

[An electron microscopic observation of the glycogen-rich adenocarcinoma of the parotid gland (author's transl)].

A case of glycogen-rich adenocarcinoma of the parotid gland of a 23 years old man was investigated ultrastructually. The cytoplasm of the tumour cells was abundantly filled with glycogen-rich particles. Among these particles well developed cytoplasmic organelles were observed, too. In addition, another tumour all type could be found, i.e. a tumour cell of basal cell origin with tonofibris in the cytoplasm, attached to the glycogen-rich tumour cell type by desmosomes.

Adenocarcinoma↗
PubMed1980

[The ultrastructure of a neurinoma derived from the facial nerve (author's transl)].

A rare case of a neurinoma of the left facial nerve was investigated on the electron microscopic level. The tumor developed in a 27 years old man and induced a facial paresis by nerve compression. Ultrastructurally the tumour cells were characterized by markedly notched nuclei and a well developed organized cytoplasm with multiple organelles (lysosomes, Golgi's complex, mitochondria, rough endoplasmic reticulum). Fibroblasts were frequently observed in the interstice. So-called "long spacing collagen" was not detected in the interstice. The arrangement of the tumour cells represents the Antoni A type.

Adult↗
PubMed1979

Postsynaptic potentials recorded from medullary neurones following stimulation of carotid sinus nerve.

Projections of the carotid sinus nerve (CSN) onto medullary neurones were studied with intracellular recording. Three types of postsynaptic potentials (EPSP, EP-IPSP and IPSP) were recorded by stimulation of the ipsilateral CSN. Of the total of 121 neurones, positions of 54 were identified by intracellular dye. The other 67 were positioned by extrapolation. They were distributed over 5 medullary nuclei: (1) nucleus of the solitary tract (NTS); (2) paramedian reticular nucleus (NPR); (3) perihypoglossal nucleus (PXII); (4) lateral tegmental field (FTL); and (5) nucleus ambiguus. Since penetration of microelectrodes and injection of dye into the NTS neurones was difficult, neurones of the other 4 nuclei were examined. The IPSPs were dominant in small NPR neurones, while the EPSPs were dominant in large neurones of the other 3 nuclei. Both the NA and PXII neurones showed forms of a motor type neurone, while the FTL neurones showed various forms. The EPSPs with onset latency as short as 2-4 msec were frequently recorded in different nuclei. This strongly suggests that the CSN projects monosynaptically onto different nuclei in the medulla.

Animals↗
PubMed1979

[Electronmicroscopic observation on acinic cell tumors of the parotid gland].

Under the electronmicroscope we observed a case of an acinic cell tumor of the left parotid gland. The patient was a 63-year-old woman who has suffered three times from local recurrences. The cytoplasma of the tumor cells showed the well-organized mitochondria, rouch endoplasmic reticula, Golgi's complexes and other organellas. Especially the cytoplasmas of the tumor cells, which formed acinus-like lumens, contained various-shaped secretory granules in great numbers. In addition, pycnotic-nucleated oncocytes with secretory granules could be observed.

Female↗
PubMed1979

Automated determination of red cell methaemoglobin reductase activity by a continuous-flow system for screening hereditary methaemoglobinaemia.

A flow diagram for the automated determination of ferricyanide reductase activity in red blood cells was prepared in the modules from AutoAnalyzer AA I (Technicon Instruments Inc). Ferricyanide reductase assay can be substituted for assay of cytochrome b5 reductase (EC 1.6.2.2), which plays a major role in reducing methaemoglobin in erythrocytes, and is defective specifically in the erythrocytes of patients with hereditary methaemoglobinaemia. The effective sampling rate of the analysis is 30/h, and less than 0.05 ml of whole blood is required. Interference of haemoglobin with absorption by potassium ferricyanide at 420 nm is effectively exculded by dialysis. This automated method was compared with the accepted diaphorase method, and it distinguished clearly the ferricyanide reductase activity of cord bloods from that of adult bloods. The activity of the blood from a patient with hereditary methaemoglobinaemia was only residual. It is suggested that the method is useful as a mass screening test for hereditary methaemoglobinaemia.

Adult↗