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Biomedical subjects

T Kinouchi

Publications and source records attributed to T Kinouchi.

At least 217 records · Page 12Linked to original sources
PubMed1982

Effect of helper T cells on prevention of tolerance induction in effector T cells responsible for syngeneic tumor immunity.

The present study investigates the role of helper T cells in preventing tolerance in effector T cells responsible for syngeneic tumor immunity. Tolerance in T cell-mediated immune responses against X5563 plasmacytoma was induced in syngeneic C3H/He mice by intravenous (iv) administration of 7,000 R X-irradiated X5563 tumor cells. Since the anti-X5563 tumor tolerance was observed irrespective of whether the tumor cells had been modified with trinitrophenyl (TNP) hapten prior to iv inoculation, the ability of TNP-reactive helper T cells to prevent the tolerance induced by iv inoculation of TNP-X5563 tumor cells was examined. TNP-reactive helper T cell activity was generated in C3H/He mice by immunization with TNP-isologous mouse gamma-globulin after inoculation with the TNP-conjugate of a non-immunogenic co-polymer of D-glutamic acid and D-lysine. Intravenous inoculation with TNP-modified X5563 cells induced tolerance in mice not primed with TNP. However, such tolerance was prevented in mice in which TNP-reactive helper T cell activity had been generated. These results indicate that helper T cells play a crucial role in preventing effector T cell tolerance. The mechanisms by which tolerance induction in effector T cells is circumvented are discussed in relation to the generation and delivery of signals required for T cell activation.

Animals↗
PubMed1982

Expression of differentiation antigens cross-reactive with Ly-6.2 alloantigens on MM102 mouse mammary tumor cells.

The present study was carried out to investigate the immunobiological nature of alien antigens which appear as tumor-associated transplantation antigens (TATA) on MM102 mammary tumor cells. Anti-MM102 tumor-specific anti-serum obtained was used for examining the expression of antigens cross-reactive with TATA of MM102 tumor on lymphoid cells from allogeneic mice. The results demonstrate that this antiserum exhibited an appreciable degree of reactivity against C57BL/6, but not against C3H/He or BALB/c spleen cells. Further analysis revealed that thymocytes and bone marrow cells were marginally susceptible to treatment with anti-MM102 antibody plus complement. In contrast, portions of peripheral T and B lymphocytes were found to exhibit such cross-reactive antigens. It was also demonstrated that the strain distribution of expression of MM-TATA-cross-reactive antigens was completely correlated with that of Ly-6.2 alloantigens. Moreover, anti Ly-6.2 monoclonal antibody lysed MM102 tumor cells in the presence of rabbit complement. These results indicate that TATA of MM102 tumor cells are cross-reactive with differentiation antigens which develop on allogeneic lymphoid cells and are closely related to Ly-6.2 alloantigens.

Animals↗
PubMed1981

Characterization of two inducible bacteriophages, alpha 1 and alpha 2, isolated from Clostridium botulinum type A 190L and their deoxyribonucleic acids.

Two inducible bacteriophages, alpha 1 and alpha 2, isolated from Clostridium botulinum type A strain 190L and their deoxyribonucleic acids (DNAs) were purified and characterized. Phage alpha 1, which is unable to form plaques on any strain of C. botulinum, was produced in large quantities after treatment with mitomycin C (MC), whereas phage alpha 2, which was induced in much lower quantities than phage alpha 1, propagated in cultures of type A strain Hall. The phage DNAs were exclusively synthesized after induction with MC. Alpha 1 and alpha 2 DNAs had sedimentation coefficients of 34.0 and 30.6 S, corresponding to molecular weights of 31.9 x 10(6) and 23.5 x 10(6), respectively. The buoyant density in CsC1 was 1.682 g/cm3 for alpha 1 DNA and 1.680 g/cm3 for alpha 2 DNA. Based on thermal denaturation characteristics, the genomes of both phages were shown to be double-stranded DNAs. Agarose gel electrophoretic profiles of the phage DNAs digested with restriction endonuclease EcoRI revealed nine fragments for alpha 1 DNA and six fragments for alpha 2 DNA. The molecular weights of the phage DNAs as determined by restriction enzyme analysis were 30.55 x 10(6) for alpha 1 DNA and 25.83 x 10(6) for alpha 2 DNA. Nontoxigenic mutants obtained from strain 190L could, like the toxigenic parent strain, produce the two phages after treatment with MC. Lysogenic conversion to toxigenicity by phage alpha 2 was not observed with the nontoxigenic mutants. It seems likely that there is no relationship between either phage genome and the toxigenicity of C. botulinum type A.

Bacteriophages↗
PubMed1981

A case of Maffucci's syndrome associated with primary hyperparathyroidism.

Maffucci's syndrome (association of multiple enchondromatosis and multiple hemangiomata) is rarely associated with other ectodermal tumors. The present case is the second in literature in which the Maffucci's syndrome is associated with the parathyroid adenoma resulting in primary hyperparathyroidism. Although the cystic lesions of the bone and the ectopic calcifications are common to both diseases, pathogenesis apparently not related. The association of parathyroid adenoma with Maffucci's syndrome suggests the potential involvement of mesenchymal organs in this ectodermal neoplastic disorder.

Adenoma↗
PubMed1980

Isolation of nontoxigenic variants associated with enhanced sporulation and alteration in the cell wall from Clostridium botulinum type a 190L by treatment with detergents.

Nontoxigenic variants were isolated from Clostridium botulinum type A strain 190L after treatment with detergents such as deoxycholate, sodium dodecyl sulfate, Tween 80 and Brij-58. Deoxycholate was most effective for obtaining the variants. The variants exhibited a markedly increased frequency of sporulation compared with the oligosporogenic parent strain. The cell wall of the parent strain was composed of an outer layer and an inner layer, whereas that of the variants lost the outer layer. After treatment with mitomycin C the parent strain was subjected to lysis and produced bacteriophages with a hexagonal head and a contractible tail, while the nontoxigenic variants did not yield bacteriophages or phage-like structures. There appears to be a close relationship among the toxigenic and sporogenic properties, formation of the outer cell wall layer and lysogeny.

Botulinum Toxins↗
PubMed1980

Ultrastructure of a hexagonal array in exosporium of a highly sporogenic mutant of Clostridium botulinum type A revealed by electron microscopy using optical diffraction and filtration.

The ultrastructure of a hexagonal array in the exosporium from spores of a highly sporogenic mutant of Clostridium botulinum type A strain 190L was studied by electron microscopy of negatively stained exosporium fragments using optical diffraction and filtration. The exosporium was composed of three or more lamellae showing and equilateral, hexagonal periodicity. Images of the single exosporium layer from which the noise had been filtered optically revealed that the hexagonally arranged, morphological unit of the exosporium was composed of three globular subunits about 2.1 nm in diameter which were arranged at the vertices of an equilateral triangle with sides of about 2.4 nm. The morphological units were arranged with a spacing of about 4.5 nm. the adjacent globular subunits appeared to be interconnected by delicate linkers.

Clostridium botulinum↗
PubMed1979

Isolation and partial characterization of exosporium from spores of a highly sporogenic mutant of Clostridium botulinum type A.

Homogeneous fragments of exosporium were isolated and purified from mature spores of a highly sporogenic mutant derived from Clostridium botulinum type A strain 190L. The exosporium was composed of three lamellae and showed a hexagonal array when negatively stained. The hexagonal array of isolated exosporium was resistant to sodium dodecyl sulfate, urea, dithiothreitol, and proteolytic enzymes such as trypsin, pronase, and nagarse, except for pepsin. The hexagonal array was partially disintegrated with 5 M guanidine-HCl and almost completely disrupted with 8 M urea in combination with 1% mercaptoethanol under alkaline conditions. The purified exosporium fraction was composed mainly of protein (69.1%) and lipids (13.8%). A small amount of amino sugars (2.5%) was present, but neutral sugars could not be detected. The exosporium protein had a predominantly acidic amino acid composition accompanied by low levels of cystine, methionine, and histidine.

Amino Acids↗
PubMed1978

Quantitative relationship between serum Ca and inorganic phosphate concentrations in uremic patients.

Sixty four blood samples from 28 uremic patients were analyzed for total calcium (Ca), inorganic phosphate (P), total protein (TP), and albumin in the serum. In these patients Ca had no significant correlation with either TP or albumin as was generally postulated, but showed significant correlation only with P. The regression analysis revealed that the residuals were of the same order of magnitude in both linear and non-linear formation. It was also found that the product, CaxP, increased along with BUN in uremic patients.

Calcium↗
PubMed1978

Sodium excretion in volume-expanded dogs. I. Comparison of the effects of physiological saline and 5% glucose solution.

Dogs were subjected to volume expansion with physiological saline and 5% glucose solution. During the infusion Na excretion was significantly increased only when saline was given. Maintenance of the volume expansion with 5% glucose solution immediately following saline infusion was ineffective in preventing the decline of Na excretion rate after the cessation of saline loading. Although the indices of hemodilution such as plasma total protein concentration and peripheral venous hematocrit were reduced along with natriuresis, these values also correlated with the total amount of Na retained during saline infusion. And this amount of retained Na has also a good correlation with the natriuresis. It is concluded that hemodilution is not the essential cause of natriuresis in the present volume expansion experiments, and that the amount of Na retained during saline loading determines the Na excretion rate in some unknown manner. The implication of regression coefficient between Na retention and Na excretion is discussed as a characteristic value in the Na homeostatic mechanism of the organism.

Animals↗