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Biomedical subjects

T Kawata

Publications and source records attributed to T Kawata.

At least 289 records · Page 16Linked to original sources
PubMed1975

Immunochemical characterization of cell wall protein antigen purified from the cell wall autolysate of Clostridium botulinum type A.

The cell wall protein antigen was solubilized from the isolated cell walls of Clostridium botulinum type A by autolysis and purified by diethylaminoethyl-cellulose column chromatography followed by gel filtration on Sephadex G-150. The two fractions showed a high degree of the serological activity and produced a main fused precipitin line in immunodiffusion tests against the homologous antiserum. The fact that antigenic fractions contained various kinds of amino acids but no detectable amounts of amino sugars or carbohydrates suggests that the antigens were principally composed of proteins. The protein antigen possessed multiple antigenic components in immunoelectrophoresis. As serological activity, the antigen was heat-stable and resistant to tryptic digestion but sensitive to the actions of pronase, nagarse or pepsin. The protein antigen appeared to be responsible for the common antigenicity among the proteolytic strains of C. botulinum.

Antigens, Bacterial↗
PubMed1975

Colony-forming ability of bone marrow cells of W anaemic mice on macrophage layer formed in peritoneal cavity of mice.

The colony-forming ability of haematopoietic cells of W anaemic mice was examined on the macrophage layer formed in the peritoneal cavity of mice. Bone marrow cells of W anaemic mice formed a considerable number of colonies on the macrophage layer, notwithstanding they did not form any colonies in the spleen of the same recipients. As the colony-forming ability of the bone marrow cells was not reduced by the incubation with 3-H-thymidine, most of the cells which formed colonies on the macrophage layer seemed to stay in G0 state. The interrelationship between the spleen colony-forming cells, the macrophage-layer colony-forming cells, and in vitro colony-forming cells was discussed.

Anemia, Macrocytic↗
PubMed1975

Factors which influence development of macrophage-layer and spleen colonies in mice.

Irradiated mice infused with bone marrow cells developed colonies both in the spleen and on the macrophage layer of the cellulose acetate membrane which had been inserted into their peritoneal cavity. Factors influencing the ratio of spleen to macrophage-layer colonies were examined. The ratio of the colonies depended on the condition of the recipients rather than the condition of the injected cells provided there was no marked histoincompatibility between donors and recipients.

Animals↗
PubMed1975

Range of xenogeneic donors whose hematopoietic cells can form colonies on macrophage layer of mice.

The colony-forming ability of hematopoietic cells derived from 18 species of rodents was examined on the mouse macrophage layer which formed on a cellulose acetate membrane inserted into the peritoneal cavity of mice. Bone marrow cells of 12 xenogeneic species made a considerable number of colonies on the mouse macrophage layer. However, there was no parallelism between the colony-forming ability of the cells and donor's taxonomic relationship to the mouse.

Animals↗