Biomedical subjects

T Katayama

Publications and source records attributed to T Katayama.

655 records · Page 37Linked to original sources

PubMedDate not supplied

Acrobeads test using monoclonal antibody-coated immunobeads: clinical application.

Acrosome-reacted sperm was examined in infertile and fertile males using the Acrobeads test. The Acrobeads score was significantly higher in fertile males than in infertile males with normal or abnormal seminal findings (p < .005 and p < .001, respectively). The Acrobeads score was 2 or more in all the fertile males, but 62% of the infertile males with abnormal seminal findings (p < .02). Therefore, a disturbance in the acrosome reaction is suggested to be one of the causes of decreased fertility potential. A high correlation was found between Acrobeads score and sperm motility (r = .46, p < .001) or motile sperm concentration (r = .51, p < .0001). The Acrobeads test, the results of which reflect the acrosome condition of the sperm, is useful in the assessment of sperm function related to fertility.

Acrosome↗

PubMedDate not supplied

Effect of varicocele on fertility potential: comparison between impregnating and nonimpregnating groups.

Sixty patients with varicocele, complaining of infertility, underwent a corrective operation. Eighteen (30%) of the 60 patients impregnated their mates (impregnating group). Sperm concentration significantly increased after the operation in the impregnating group (p < .05), but not in the patients who failed to impregnate their mates (nonimpregnating group). Sperm motility was not altered after the operation in these two groups. Sperm velocity significantly increased postoperatively in the impregnating group (p < .05), while linearity was not altered in either group. Sperm swelling significantly increased postoperatively in the impregnating group (p < .05), but not in the nonimpregnating group. Preoperative sperm parameters were not different between the two groups. Varicocele may disturb sperm function including sperm motion besides spermatogenesis and may impair the fertility potential. However, a prospective large-scale controlled study is required to obtain a definitive conclusion.

Adult↗

PubMedDate not supplied

[A case of the cerebellar atrophy and pseudo-hyperchloremia as a clue to diagnose chronic bromvalerylurea intoxication].

A 36-year-old woman was admitted to our hospital because of walking disturbance and dizziness. Her neurological examination showed psychiatric disturbance, truncal and limb ataxia, wide-based gait. She has taken 3 g of bromvalerylurea daily. The laboratory test revealed elevated levels of serum chloride (201 mEq/L) and bromide (105 mg/dl), and decreased (-43 mEq/L) anion gap. Brain MRI revealed atrophy of upper part of the vermis and cerebellar hemisphere, and widening of the primary fissure and dilatation of the forth ventricle. Bromvalerylurea, which is an easily available sedative, should be still noted as a cause of cerebellar ataxia. Increased level of serum chloride was useful information for early diagnosis of chronic bromide intoxication.

Adult↗

PubMedDate not supplied

Hypolipidemic action of phytic acid (IP6): prevention of fatty liver.

Until recently, most published reports on phytic acid (myo-inositol hexaphosphoric acid, IP6) have focused on the possible decreased mineral bioavailability. Because myo-inositol is known to function as a lipotropic factor, studies in my laboratory were conducted to investigate whether dietary IP6 also reduces excessive liver lipids. Male Wistar rats were fed sucrose or corn starch diets, supplemented with myo-inositol or IP6 for 12-14 days. Equimolar myo-inositol and IP6 similarly depressed the rises in hepatic levels of lipids and in hepatic activities of lipogenic enzymes due to sucrose feeding. However, dietary myo-inositol and phytate did not prevent orotic acid-induced hepatic lipid accumulation, which is known to be caused by severe inhibition of hepatic lipoprotein secretion. These results suggest that myo-inositol and phytate might both protect against fatty liver resulting from elevated hepatic lipogenesis.

Animals↗

PubMedDate not supplied

Modification of apoptosis-inducing activity of gallic acid by saliva.

Gallic acid, a structural unit of tannin, induced apoptotic cell death, characterized by nuclear condensation and caspase activation in human oral tumor cell lines (HSC-2, HSG). Agarose gel electrophoresis demonstrated that gallic acid produced large DNA fragments in these cells, as well as in T-cell leukemia (MOLT-4) and erythroleukemia (K-562) cells, whereas it induced internucleosomal DNA fragmentation in human myelogenous leukemic cell lines (HL-60, ML-1, U-937, THP-1). This indicates that induction of internucleosomal DNA fragmentation or production of large DNA fragments depends on both target cells and inducers. Addition of total saliva dose-dependently reduced the cytotoxicity induction by gallic acid. These data suggested that the biological action of gallic acid might be modified by physiological fluids.

Antioxidants↗

PubMedDate not supplied

Correlation between the hypoosmotic swelling test and various sperm function tests.

OBJECTIVE: To compare the hypoosmotic swelling test (HOST) with other sperm function assays. MATERIALS AND METHODS: The HOST was performed on 105 patients with idiopathic infertility. The results were compared with those of various other sperm function tests, including the sperm penetration assay (SPA), triple-stain technique for evaluating acrosomal reactions, and the in vitro cervical mucus penetration test (Penetrak). RESULTS: A strong positive correlation was observed between percent sperm motility and the percent swollen sperm, between the motile sperm concentration and sperm swelling, and between sperm swelling and penetration values. Only a weak correlation was detected between sperm swelling and the percent sperm penetration. However, most of the cases with a sperm swelling percentage of 50% or more had good sperm penetration into ova. CONCLUSIONS: The HOST was useful to evaluate sperm tail function and was found valuable as a screening test prior to the SPA.

Adult↗

PubMedDate not supplied

Comparison of resazurin test results with various sperm parameters.

The resazurin reduction test was performed on semen samples from 83 untreated infertile and 9 pregnancy-confirmed fertile males, and the results were compared with other sperm parameters. The highest correlations with resazurin grade were found for motile sperm concentration (r = 0.65, P < 0.001), per cent motility (r = 0.58, P < 0.01), and percentage of swollen sperm (r = 0.57, P < 0.01). There were also significant but weak correlations between resazurin grade and both sperm concentration and linearity. There was a significant difference in the rate of positive tests between samples from normozoospermic and fertile males, and samples from patients exhibiting abnormal semen parameters (P < 0.001). These findings indicate that the resazurin test may be able to predict those males who may be fertile despite an abnormal semen analysis, and therefore, the test may be useful in the evaluation of the infertile patient as a part of a comprehensive male fertility assessment.

Adult↗