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T Kanno

Publications and source records attributed to T Kanno.

At least 433 records · Page 24Linked to original sources
PubMed1988

K channels of human alveolar macrophages.

The activation of macrophages has been reported to be associated with Ca-activated K permeability change. In order to study this permeability change in human alveolar macrophages, we examined alveolar macrophages electrophysiologically at a single channel level. We observed two types of Ca-activated K channel currents having conductances of 218 +/- 2 and 32 +/- 0.6 picosiemens in symmetrical 154 mmol/L KCl solutions. The characteristics, such as voltage dependency and Ca sensitivity, as well as channel conductance, were different between these two types of channel currents. Quinine (a blocker of Ca-activated K conductance), 0.5 mmol/L, reduced these channel currents by 45 +/- 8% and 31 +/- 8%. Quinine, 0.5 mmol/L, also inhibited chemiluminescence and leukotriene B4 release by 82 +/- 6 to 88 +/- 3% and 88 +/- 2%, respectively. These results suggest the presence of two types of Ca-activated K channels, which may be related to the release of inflammatory mediators from human alveolar macrophages.

Calcium↗
PubMed1988

Isoenzymes of alkaline phosphatase in germinoma cells.

Utilizing 18 germinomas, the characteristics of isoenzymes of alkaline phosphatase (ALP) in germinoma cells were examined by light and electron microscopic enzymohistochemical and immunohistochemical techniques and biochemical methods including inhibition tests, electrophoresis and electrosyneresis. ALP in germinoma cells was generally located on the cell surface. In a few germinoma cells, however, ALP was seen not only on the cell membrane but also in the endoplasmic reticulum. Enzymohistochemically as well as biochemically, ALP in germinoma cells was little sensitive to L-phenylalanine, moderately sensitive to L-homoarginine and somewhat resistant to heat in its inhibition tests. Immunohistochemical examinations and electrosyneresis showed the presence of the placental type of ALP in germinoma cells and biochemical analyses revealed that the heat-stable component of ALP in germinoma cells was consistent with the D-variant of the placental type of ALP. Therefore, germinoma cells possessed mixed isoenzymes of ALP, consisting mainly of the liver/bone type and a small amount of D-variant of the placental type on the cell surface. The prominent expression of ALP in germinoma cells may be due to the enhanced expression of gonadal genes active in the germinoma genome.

Adult↗
PubMed1988

Optical absorbance changes induced by CCK-8 under limited O2 supply in isolated perfused rat pancreas.

Optical absorbance spectra were recorded simultaneously and continuously in the isolated rat pancreas arterially perfused at various flow rates. This was done to explain how optical absorbance changes corresponding to parallel reduction of cytochromes aa3, b, and cc1 were observed in perfused pancreas stimulated with high concentration of an exocrine secretagogue such as cholecystokinin-(26-33) (CCK-8). With perfusion flow rate between 1.5 and 3.0 ml/min, there were no optical absorbance changes corresponding to cytochrome reduction, but these optical absorbance changes occurred when the perfusion flow rate was decreased to 1.0 ml/min. These optical absorbance changes were not observed during exocrine secretion stimulated by CCK-8 (30 pM, 100 pM, and 1 nM) at the perfusion flow rate of 3.0 ml/min. Transient but slight changes in optical absorbances, which correspond to reduction of cytochromes, were observed in the glands perfused at the flow rate of 2.0 ml/min when secretion was stimulated by 1 nM CCK-8. When the perfusion flow rate was decreased to 1.0-1.5 ml/min, these optical absorbance changes corresponding to reduction of cytochromes occurred in glands stimulated by CCK-8 (30 pM, 100 pM, and 1 nM). Optical absorbance changes corresponding to reduction of mitochondrial cytochromes during secretion stimulated with CCK-8 may indicate local hypoxia in the perfused organ.

Animals↗
PubMed1988

Phosphorus nuclear magnetic resonance in isolated perfused rat pancreas.

Phosphorus nuclear magnetic resonance spectroscopy was applied to measure phosphorus energy metabolites in isolated perfused rat pancreas. The gland was perfused with a modified Krebs-Henseleit solution at room temperature (25 degrees C). 31P resonances of creatine phosphate (PCr), ATP, ADP, inorganic phosphate (Pi), and phosphomonoesters (PMEs) were observed in all the preparations of pancreas. In different individual preparations, the resonance of PCr varied, but those of ATP were almost the same. The initial levels of PCr and ATP in individual preparations, however, remained almost unchanged during perfusion with the standard solution for 2 h. When the perfusion was stopped, the levels of ATP and PCr decreased, while the levels of PME and Pi increased. At that time, the Pi resonance shifted to a higher magnetic field, indicating that the tissue pH decreased. On reperfusion, the tissue levels of phosphorus compounds and the tissue pH were restored to their initial resting levels. Continuous infusion of 0.1 microM acetylcholine caused marked and sustained increases in the flow of pancreatic juice and protein output. During the stimulation the tissue levels of phosphorus compounds remained unchanged, while the tissue pH was decreased slightly (0.05 pH unit).

Adenine Nucleotides↗
PubMed1988

[Evaluation of transcatheter arterial embolization in terms of serum alpha-fetoprotein doubling time in hepatocellular carcinoma].

We measured alpha-fetoprotein doubling time (T2AFP) in 51 patients with hepatocellular carcinoma treated by transcatheter arterial embolization (TAE). We also studied vascular invasion and the survival rate, and looked for correlations. For six patients with T2AFP less than or equal to 30 days after TAE, the time until the finding of vascular invasion by ultrasonography and angiography was 8.5 +/- 3.9 months; for seven patients with T2AFP greater than or equal to 31 days after TAE, it was 17.6 +/- 5.2 months (p less than 0.01). Of 45 patients for whom T2AFP was measured after TAE, 9 were in group A with T2AFP greater than or equal to 61 days, 18 were in group B with an intermediate value, and 18 were in group C with T2AFP less than or equal to 30 days. The two-year survival rate after the first TAE was 55.6% in group A, 42.5% in group B, and 6.0% in group C. The longest survival period was 51 months in group A, 30 months in group B, and 28 months (patient still alive) in group C. In the 18 patients for whom T2AFP was measured before and after TAE, the T2AFP was markedly decreased after TAE in 7 (39%). The pattern of change in T2AFP after TAE was not clearly correlated with the decrease in AFP or the T2AFP value before TAE.

Carcinoma, Hepatocellular↗
PubMed1988

[The role of antineoplastic solution in transcatheter hepatic arterial embolization--in terms of survival rates affecting anticancer agents together with gelatin sponge].

In a multicentered randomized prospective study, hepatic arterial embolization using gelatin sponge (GS) alone or together with anticancer agents was performed in a total of 112 patients with hepatocellular carcinoma to ascertain the clinical availability of the anticancer agents combined with gelatin sponge. Envelopes, each of which contained a note mentioning one of the following three kinds of regimens, were allocated at random to the participating institutes: 1) GS + mitomycin C (MMC) 10 mg + adriamycin (ADR) 20 mg (group A, n = 45), 2) GS + MMC 10 mg + ADR 20 mg + 200 mg/day of oral 5-fluorouracil (5-FU) (group B, n = 36), and 3) GS + 200 mg/day of 5-FU (group C, n = 31). The envelopes so allocated were opened just prior to embolization, and the treatment was carried out as instructed. Survival rates were estimated according to the method of Kaplan-Meier for comparative analysis. The survival rate was significantly higher in group A than in group C on days 465 (p less than 0.05). It was also significantly higher in group B than in group C on days 279, 558 and 651 (p less than 0.05) as well as on days 372 and 465 (p less than 0.01). These observations suggest that this embolization using anticancer agents such as MMC and ADR, compared with the embolization using gelatin sponge alone, may exert a life-prolonging effect in patients with hepatocellular carcinoma.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1988

[Effects of neurostimulation on the reversibility of neuronal function: experience of treatment for vegetative status].

UNLABELLED: Developments in the diagnostic procedures, microsurgical techniques, intensive care, and the social rescue system have lowered the mortality in cerebrovascular diseases, head injuries and other emergencies of neurology and neurosurgery. However, this decreased mortality has given rise to a new social and medical problem i.e. vegetative status, which is an increase in the number of people who live on with mild or severe residual neurological deficits. During the last two years, we have used dorsal column stimulation (DCS) for improving spasticity of hemiplegics. While doing so, we incidentally found an improvement in the EEG in some of the cases. This led us to use dorsal column stimulation in cases of vegetative status. In this paper, we describe methodology and our experience with ten cases of vegetative status. All cases satisfied the definition of "vegetative status" and had received medical treatment for more than 3 months prior to DCS without showing any clinical improvement. A neurostimulator made by "Medtronic Neuro" was used. The neurostimulator was implanted in epidural space, in midline, at C-2 level under general anaesthesia. Video recording of clinical conditions, EEG, rCBF and catecholamine metabolism in CSF at regular intervals were monitored to see the efficacy of this treatment. RESULT: 1) There was an improvement in the EEG in nine cases out of ten cases after neurostimulation. This was in the form of appearance of more wide spread alpha-waves, and was noticed between 3 to 14 days after starting the neurostimulation. 2) Four of these cases showed good clinical improvement. In the other four cases there was slight clinical improvement.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1988

Results of transtrochanteric rotational osteotomy for nontraumatic osteonecrosis of the femoral head.

From 1976 to 1985, transtrochanteric rotational osteotomy was applied to 58 hips of nontraumatic necrosis of the femoral head. Of these, 52 hips were available for the present study. The follow-up periods ranged from one to ten years (average, 5.1 years). Satisfactory results were obtained in 69%, as there was no evidence of progressive collapse of the head. Hips that had had no collapse or slight collapse before osteotomy had a significantly better prognosis, compared with those that had marked collapse of the head. The extent of the lesion as depicted on the preoperative lateral roentgenogram also influenced the eventual function. Patient satisfaction was significantly greater when these lesions were less than 70% (31/37), compared with greater than 70% (5/15).

Adult↗
PubMed1987

Cytochrome reduction induced by increase in extracellular potassium and by electrical stimulation in isolated perifused neurohypophysis of guinea pig under mild hypoxic conditions.

Parallel reduction of cytochromes, a(a3), b and c + c1 was recorded under mild hypoxic conditions when the neurohypophysis was electrically stimulated and extracellular KCl concentration was increased. The electrically stimulated reduction was abolished by tetrodotoxin and by replacement of NaCl with LiCl. The reduction was not influenced by removal of extracellular Ca2+, or by application of a Ca ionophore, A23187. These results show that cytochrome reduction correlates with K+-influx in the resting state, and with Na+-influx followed by activation of the neurosecretory terminals contained in the neurohypophysis.

Animals↗
PubMed1987

The treatment of dural AVM by embolization with aron alpha (ethyl-2-cyanoacrylate).

The authors report four cases of dural arteriovenous malformation (AVM) treated by a modified technique of embolization. Three cases had posterior fossa dural AVM and one had bilateral supratentorial dural AVM. The authors think that the best way to treat dural AVM is to close its nidus completely. This could be achieved by a safe and easy technique of embolization. The material used for embolization should intimately adhere to the vascular network and should not become fragmented. We used aron alpha (Ethyl-2-cyanoacrylate). This is a biological glue and it sets in a solid membranous form instantaneously on coming into the contact with an ionic medium. It does not get fragmented. Aron alpha was injected into the nidus of the AVM through a cannula inserted only into the main feeding artery in four cases of dural AVM reported here. Postoperative angiography showed complete obliteration of the AVM's, they did not fill either from the treated artery or from the untreated feeding arteries. The technique is easy and safe. There was no operative or postoperative complication. All the patients have remained asymptomatic to date, i.e. 2, 2, 6 and 7 1/2 years after the procedure.

Adult↗
PubMed1987

An experimental model of communicating hydrocephalus in C57 black mouse.

The authors report a new experimental model for the study of hydrocephalus. Hydrocephalus was produced in C57 black mouse by transplanting human glioma cultured cells. 20% of the animals developed hydrocephalus while the incidence of spontaneous hydrocephalus is only 1%. The transplanted cells disappeared within 72 hours. The mouse developed progressive hydrocephalus. There was no evidence of blockage of CSF pathways. The only abnormal microscopic finding was a scattered collection of lymphoid cells in some of the animals, which may be a hypersensitivity reaction to the cells. A similarly hypersensitive reaction to the foreign maternal protein which may enter the foetal circulation is suggested as the cause of hydrocephalus in some of the cases of congenital hydrocephalus.

Animals↗