Search PubMed⌕ Search

Biomedical subjects

T Kamata

Publications and source records attributed to T Kamata.

At least 235 records · Page 13Linked to original sources
PubMed1981

Role of prostaglandin E2 on gastric ulcers induced by non-steroidal anti-inflammatory compounds (NOSAC).

We investigated the effect of indomethacin and aspirin on the prostaglandin E2 (PGE2) levels in rat gastric mucosa. In untreated controls, the PGE2 level was 1085 +/- 80 ng/g in the fundic- and 2435 +/- 339 ng/g in the antral mucosa; it was markedly reduced by indomethacin (6 or 20 mg/kg) in a dose-related manner prior to the formation of gastric ulcers. Aspirin (200 mg/kg) also decreased the mucosal PGE2 content. The simultaneous infusion of 5 microgram/kg 16, 16-dimethyl PGE2 and 20 mg/kg indomethacin inhibited the formation of gastric ulcers. Our experimental results suggested that the indomethacin- or aspirin-induced gastric mucosal lesions are due to a deficiency of endogenous mucosal PGE2.

Animals↗
PubMed1981

[Role of complement for the sensitivity of sperm immobilization test (author's transl)].

For detecting the sperm immobilizing antibody in the sera of infertile women with unknown cause, Isojima's sperm immobilization test was widely used, but there was great difference on the percentage of positive results among several laboratories. For analysis of factors influencing the sensitivity of the test, source and amount of complement were studied, and the following results were obtained. 1. Guinea pig serum which contained more than 200 CH50 as hemolytic titer was best for this test. 2. Rat and rabbit sera which contained much lower CH50 than guinea pig indicated relatively good sensitivity for the test. 3. Human serum which contained 34.9 CH50/ml and mouse serum which contained less than 5 CH50/ml, both could not work on sperm immobilization test. The human serum (AB) which was concentrated to the level of guinea pigs serum as CH50 did not work well on sperm immobilization test as guinea pig serum. 4. When the complement from same source was used, the positive correlation between CH50 (hemolytic titer) and 50% sperm immobilization titer (SI50) was present, but if the complement source was different, there was no correlation between CH50 and SI50. 5. It was proved that more than 10 CH50 of guinea pig serum was necessary for sperm immobilization test to obtain the most sensitive results.

Animals↗
PubMed1981

[Comparative studies on doses of complement for detection of sperm immobilizing antibody in serum, cervical mucus and seminal plasma (author's transl)].

In Isojima's sperm immobilization test, the complement is one of the most important factors which influence on sensitivity of the test. Previously we reported that more than 10 CH50 of guinea pig serum complement was necessary in the reaction to obtain the best result for measuring the sperm immobilizing antibody in the serum. This time, the influences of various test specimens on complement activities for the sperm immobilization test were studied and the necessary amounts of complement to detect the sperm immobilizing antibody in serum, cervical mucus and seminal plasma were compared. In the sperm immobilization test, the following results were obtained: 1. The amount of complement (guinea pig serum) added was 11.9 CH50, and after 60 minutes incubation at 32 degrees C in veronal buffer (VB2+), 2.0 +/- 0.3 (M +/- SD) of CH50 was consumed. 2. When human serum, cervical mucus and seminal plasma were added to the reaction as specimens, the complement consumptions (M +/- SD) were more 0.2 +/- 0.3 CH50, 0.2 +/- 0.2 CH50 and 3.3 +/- 0.8 CH50 than that of VB2+ buffer respectively. 3. When the washed human spermatozoa were added, the consumed amounts of complement (M +/- SD) were dose dependent; 3.5 +/- 0.7 CH50 for 100 X 10(4) spermatozoa, 7.3 +/- 0.3 CH50 for 200 X 10(4) spermatozoa and 15.7 +/- 2.2 CH50 for 400 X 10(4) spermatozoa. 4. In our sperm immobilization test, the net consumptions of complement (M +/- SD) were 5.0 +/- 0.7 CH50 for specimen of serum, 4.9 +/- 0.8 CH50 for specimen of cervical mucus and 11.1 +/- 1.2 CH50 for specimen of seminal plasma. 5. Complement doses necessary to obtain the most sensitive sperm immobilization tests were 10 CH50 for both serum and cervical mucus specimens, and 15 CH50 for seminal plasma specimen.

Animals↗
PubMed1979

Studies on hemorrhagic tendency in cirrhosis of the liver.

Recently it has been paid attention whether the liver diseases might induce the disseminated intravascular coagulation (DIC) or not, although it has well known that liver cirrhosis contribute to the hemorrhagic diathesis. In this paper, we studied on the hemorrhagic tendency and hemostatic tests in the patients with liver cirrhosis in order to clarify the coagulation and fibrinolytic activity. We can conclude that the hemorrhagic diathesis occurs due to the complex of decreases in platelet number, platelet functions, clotting factors, and increased fibrinolytic activity. The increased fibrinolytic activity may be primary and the frequency of clinically important DIC is quite low.

Adult↗
PubMed1979

Etiology of cholesterol gallstones.

Formation of pure cholesterol stones is initiated by an excessive intake of highly purified carbohydrates, a large intake of animal fats and a restricted intake of vegetable fibers. When the protein content of the lithogenic diet was reduced, mixed or combined stones were formed in golden hamsters. These experimentally, dietarily produced gallstones had compositions and fine structures similar to those of human gallstones. Some mentions were made on black stones which were found in the aged golden hamsters fed with lithogenic diets.

Animals↗