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T Kadota

Publications and source records attributed to T Kadota.

178 records · Page 10Linked to original sources
PubMed1976

Coated-vesicle shells, particle/chain material, and tubulin in brain synaptosomes. An electron microscope and biochemical study.

Coated vesicles (CVs), plain synaptic vesicles (PSVs), and nonvesicular flocculent material were isolated from synaptosomes and examined with goniometry and high-resolution electron microscopy after either negative staining or various biochemical procedures. The flocculent material (i.e. the presynaptic matrix material except CV shells) is largely composed of particulate or elongated (chainlike) structures; some of this material (here referred to as particle/chain material) is attached to PSVs. The results obtained were: (a) the proteinaceous properties of the CV coat (also referred to as CV shell) and the particle/chain material were demonstrated with chymotrypsin; (b) the CV shell, studied with various negative-staining techniques, differs from the particle/chain material since it has no 3-4-nm globular subunits and reacts differently to alkaline pH; (c) the particle/chain material consists of aggregates of 3-4-nm globular subunits, four of which yield 8-10-nm fine particles; and these particles can be further aggregated into chains 8-10 nm wide and up to 30-60 nm long showing a "hollow" core; (d) vinblastine sulfate induced ringlike or helical crystalloid precipitates closely resembling the vinblastine-induced microtubule crystals reported in the literature, but vinblastine had no effect on either the CV shell material or the particle/chain material.

Animals↗
PubMed1973

Isolation of coated vesicles, plain synaptic vesicles, and flocculent material from a crude synaptosome fraction of guinea pig whole brain.

Two vesicular fractions and one nonvesicular fraction were prepared from crude synaptosomes by differential centrifugation and salting out with ammonium sulfate. Fraction 1 contained a mixture of coated vesicles, material thought to be derived from breakdown of the coats (shell fragments), and plain synaptic vesicles. Fraction 2 contained a mixture of plain synaptic vesicles and flocculent material. Fraction 3 contained flocculent material only. Fractions 1 and 3 were partially purified by passage through a Sephadex column. Fraction 3 contained no shell fragments but contained finer flocculent material which, it is suggested, is composed of unit particles either occurring singly or linked together into chainlike or amorphous aggregates. Each unit particle appears to have four subunits and is here referred to as a tetrasome. Tetrasomes sometimes appear to be attached to the surfaces of the plain synaptic vesicles. Also, it is possible that aggregates of tetrasomes form part of the structure of the presynaptic dense projections.

Animals↗
PubMedDate not supplied

Pneumatosis cystoides intestinalis: CT findings.

Two patients with primary pneumatosis cystoides intestinalis are presented. In both cases, the sigmoid colon was involved and there were no major complications. On computed tomography (CT), at lung window settings, the gas cysts in the colonic wall were clearly delineated. CT was useful in confirming the intramural gas cysts in patients with primary pneumatosis cystoides intestinalis.

Colon, Sigmoid↗
PubMedDate not supplied

MR features of various histological grades of small hepatocellular carcinoma.

Twenty small hepatocellular carcinomas (HCCs) < 20 mm in diameter in 18 patients, detected by MRI, were surgically resected and histologically confirmed. Quantitative analysis of signal intensity on MR images was performed with 14 homogeneous lesions. In the five grade I HCCs, T1-weighted SE sequences provided significantly higher mean tumor-liver contrast and contrast-to-noise ratio (C/N) than T2-weighted SE sequences (p < 0.01). However, in the other nine HCCs (grade I + II or greater), T2-weighted SE sequences had significantly higher mean tumor-liver contrast and C/N values than T1-weighted SE sequences (p < 0.001). The T2-weighted SE sequences appear superior to T1-weighted SE sequences for grade I + II or greater HCC detection, whereas T1-weighted SE sequences are valuable in the detection of grade I HCCs.

Aged↗