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Biomedical subjects

T Kachi

Publications and source records attributed to T Kachi.

At least 73 records · Page 4Linked to original sources
PubMed1986

The physiology of orthostatic tremor.

This article describes the physiological findings in a patient with orthostatic tremor. The tremor primarily affected the legs, was alternating between antagonist muscle groups, and had a remarkably rapid frequency of 16 Hz. It was present only during certain postures and appeared to be of central origin. A tremor of the same frequency was also recorded in the arms during particular movements, but was cocontracting. We suggest that orthostatic tremor may be generated by spontaneous oscillation in those central structures responsible for organizing the motor programs for standing.

Electromyography↗
PubMed1986

Electrophysiology of the corticomotoneurone pathways in patients with movement disorders.

The corticomotoneurone pathways were examined in 21 patients with movement disorders, using the technique of percutaneous electrical stimulation of the motor cortex. Conduction in these pathways was assessed by measuring the latency to onset of electromyographic activity in the muscles of the upper limb after cortical stimulation. In all patients [five with primary (idiopathic) torsion dystonia and two with secondary (symptomatic) hemidystonia, seven with Huntington's disease, four with essential tremor, and three with Parkinson's disease] central motor conduction was normal. This and other evidence suggests that the origin of the disorder of movement in these conditions lies in the delivery of abnormal motor commands to a normal corticomotoneuronal system.

Adult↗
PubMed1985

Quantitative ultrastructural analysis of differences in exocytosis number in adrenomedullary adrenaline cells of golden hamsters related to time of day, pinealectomy, and intracellular region.

This research analyzed differences mainly in the incidence of exocytotic figures in adrenaline cells (A-cells) in pinealectomized (PX), sham-operated (SPX), and non-operated (NO) adult male golden hamsters, with the aim of determining whether these parameters change with the time of day and following pinealectomy, and whether intracellular regional differences exist in such changes. Animals acclimated to a standardized light:dark (LD) 12:12 photoperiod were sacrificed at 11 h after the onset of light (L-11h) and 1 h after the onset of darkness (D-1h) (8 animals/group/time) at 28 days postoperation. The adrenal medullas were examined and analyzed morphometrically by electron microscopy. The number of exocytoses per unit length (NEL) and the exocytosis index (a rough index of the number of exocytoses per cell) were measured in PF (perivascular-space-facing) and non-PF plasma membranes. NEL increased from L-11h (NO: 0.040 +/- 0.010, mean +/- SE) to D-1h (0.078 +/- 0.012) in all three experimental groups (ANOVA: P less than 0.005), showing over fourfold higher levels in PF than in non-PF membranes. NEL in PF membranes in PX animals showed higher levels than those in NO and SPX animals (P less than 0.025), but in non-PF membranes, no differences owing to time of day or surgery were seen. Exocytosis indices were (1) higher at D-1h than at L-11h in all three experimental groups (P less than 0.005), (2) similar in PF and non-PF membranes in control groups, and (3) higher in PF membranes in the PX group than in either non-PF membranes or PF membranes in control groups. In conclusion, the exocytosis number in A cells changes in relation to time of day, rising in early dark phase, and its rise following pinealectomy can be seen only in PF membranes.

Adrenal Medulla↗
PubMed1985

Writing tremor: its relationship to benign essential tremor.

Nine patients with tremor on writing and one patient with tremor only on swinging a golf club were investigated. None of the patients had any other neurological symptoms or signs. The frequency of the tremor ranged from 5 to 6 Hz. Rapid passive supination or pronation of the forearm by a torque motor evoked a short burst of alternating tremor in seven patients. The tremor was improved by alcohol or propranolol in six patients. These characteristics of writing tremor (and of other isolated action tremors) suggest that it is a variant of benign essential tremor.

Adult↗
PubMed1984

Duration of the first agonist EMG burst in ballistic arm movements.

In normal subjects, fast arm movements are accomplished by a diphasic or triphasic EMG activation of the agonist and antagonist muscles. The duration of the first agonist burst (Ag1) has been said to be constant for movements of different size, whilst its amplitude is variable. Previous studies focused on relatively small movements (10-40 degrees). We have studied the behaviour of the Ag1 duration over the full physiological range of wrist and elbow flexion movements in normal subjects. The results showed that the principle of invariance of Ag1 duration was true when small movements of about 15-30 degrees were studied, but when larger movements were made burst length increased. A similar increase in Ag1 burst duration also was seen during movements performed against a load and in contractions made with fatigued muscles. Changes in duration of the Ag1 burst appear to be part of the normal mechanism for increasing the impulsive force provided in rapid contractions.

Adult↗
PubMed1984

Quantitative cytological analysis of functional changes in adrenomedullary chromaffin cells in normal, sham-operated, and pinealectomized rats in relation to time of day: I. Nucleolar size.

Adrenaline (A)- and noradrenaline (N)-cells in juxtacortical and central areas of adrenal medullas of nonoperated, sham-operated, and pinealectomized male rats (n = 125) were investigated by quantitative electron and light microscopy. Animals were killed at eight time points during a standardized 24-h, light-dark (12:12) cycle 14 d after surgery. Diameters of nucleoli (n = 9,600) and the nucleolar margination rate were studied chiefly by light microscopy. Major findings include the following: Twenty-four-hour changes occurred in nucleolar diameters in adrenomedullary chromaffin cells in nonoperated animals, with the peak in the late dark phase (X +/- SD, A-cells: 1.29 +/- 0.06 micron; N-cells; 1.23 +/- 0.05 micron) and the minimum in the mid-light (A-cells: 1.16 +/- 0.04 micron) or early dark (N-cells: 1.14 +/- 0.03 micron) phase. These changes were markedly dampened (diminished) in sham-operated animals. Since this dampening of time-of-day changes after sham-operations was not seen in A-cells after pinealectomy, mediation by the gland of this dampening is suggested. Pinealectomized animals showed 24-h changes in nucleolar diameters, but these changes differed from those of nonoperated controls in their temporal and phase relations. For example, there was phase advance in A-cells in the juxtacortical area and desynchronization in N-cells. Furthermore, rhythm amplitude was slightly increased in A-cells and slightly decreased in N-cells after pinealectomy. A- and N-cells differed from each other consistently and significantly in the quantitative morphology of their nuleoli and nuclei, and slightly in the patterns of their time-of-day changes in nucleolar size. Nucleolar size usually showed a tendency for a regional gradient, decreasing slightly from periphery toward the center. These observations contribute to our understanding of the chronobiology and functional relations of adrenomedullary chromaffin cells, and of the effects of pinealectomy and sham operations upon this system.

Adrenal Medulla↗
PubMed1984

Seasonal changes in glycogen level and size of pinealocytes of the white-footed mouse, Peromyscus leucopus: a semiquantitative histochemical study.

Glycogen level in and size of pinealocytes of the feral, white-footed mouse Peromyscus leucopus, were studied by a semiquantitative histochemical method to determine whether seasonal changes exist in them under natural conditions, what temporal pattern they exhibit, and whether 24-hour changes in these parameters exist in different seasons, as shown in the laboratory dd-mice. Marked seasonal changes were seen in both glycogen levels and nuclear densities (ANOVA p less than 0.005). The size of pinealocytes at 09:00 to 10:00 showed one peak (and nadir) seasonal change, with the smallest size in winter (December and February) and a larger size in warmer seasons, with the maximum value in July. Glycogen level in pinealocytes at 09:00 to 10:00 showed bimodal seasonal changes, with lower levels in fall and spring and higher levels in winter and summer. In fall, a circadian trend in glycogen level in pinealocytes was seen, with a higher level at the end of the light period. In winter, the glycogen levels were very high at 09:00, 13:00, 17:00, and 21:00 examined and showed dampening of time-of-day differences. On the other hand, the size of pinealocytes followed a time-of-day change (P less than 0.005), being largest at 13:00 and smallest at 21:00. Thus, marked changes in quantitative structure and chemical activities, suggesting changes in functional activity, in pinealocytes were noted especially in severe, cold winter.

Acclimatization↗
PubMed1980

Adrenal and hypothalamic dopamine-beta-hydroxylase activity in the hamster: evidence for species-distinctive day-night and post-pinealectomy differences in activity.

Dopamine-beta-hydroxylase (DBH) activities were measured in the adrenal gland and hypothalamus of the golden hamster (Mesocricetus auratus) by means of a sensitive radioenzymatic method. Groups of unoperated control (C), sham pinealectomized (SPX), and pinealectomized (PX) adult males were sacrificed shortly before (09:00 h) and after (11:00 h) the onset of darkness of the daily photoperiod of LD 12:12 (lights on 22:00 h to 10:00 h). In neither adrenal gland nor the hypothalamus were there any day-night changes in the C groups. Pinealectomy led to no significant differences in hypothalamic DBH activity either in light or in dark. However, intracranial surgery resulted in increased DBH activity in the adrenal gland, evident in both light and dark. Although this increase was greatest in the PX groups, intracranial surgery in SPX groups also showed a trend of increase in adrenal DBH, evident even one month after surgery. Such an increase, however, was not found in hypothalamic DBH in the same SPX groups. In both the adrenal gland and the hypothalamus, copper-sensitive endogenous inhibitors of DBH did not show any notable change in concentration, either in relation to the daily light-to-dark change or as a consequence of intracranial surgery. These and related results suggest that important interspecies variations exist in DBH activities in relation to diurnal changes, and also in control or modulation by pineal and other factors.

Adrenal Glands↗
PubMed1980

The invagination complex in nerve endings on adrenomedullary adrenaline cells: quantitative ultrastructural description, and analysis of changes with time-of-day and their modification by sham-surgery and pinealectomy.

This work describes a special ultrastructural feature, the invagination complex (IC), involving the plasma membrane of nerve endings on adrenomedullary adrenaline cells. Quantitative characteristics of the IC and their changes were studied in 122 male albino (Holtzman strain) rats in 3 surgical groups: normal (non-operated, NO) sham-operated (SO) and pinealectomized (PX), all maintained in a standardized daily photoperiod (light : dark 12 : 12 h). Animals were decapitated 14 days postsurgery and at 8 specific times during the light : dark cycle. Left adrenal glands were removed, dn strain) rats in 3 surgical groups: normal (non-operated, NO) sham-operated (SO) and pinealectomized (PX), all maintained in a standardized daily photoperiod (light : dark 12 : 12 h). Animals were decapitated 14 days postsurgery and at 8 specific times during the light : dark cycle. Left adrenal glands were removed, dn strain) rats in 3 surgical groups: normal (non-operated, NO) sham-operated (SO) and pinealectomized (PX), all maintained in a standardized daily photoperiod (light : dark 12 : 12 h). Animals were decapitated 14 days postsurgery and at 8 specific times during the light : dark cycle. Left adrenal glands were removed, dissected and prepared for electron microscopy. In section profiles the diameter of each IC was usually 0.12-0.40 micrometers, and the depth 0.2-1.0 micrometers. They were frequently seen to be located near the synaptic complex (or the active zone). Coated pits, about 50 nm wide and 60 nm deep, often opened near the bottom of the invaginations of the IC. In NO animals, relative number and depth of the ICs showed daily rhythmic changes with minimal values about 1 h after onset and maximal (acrophase) values 3--5 h later (P less than 0.02 to less than 0.005, depending on index or measure). These changes occurred 3--5 h earlier, but less apparently, in SO animals, and appeared to be more greatly modified and dampened in PX animals. Two-way analysis of variance (ANOVA) and t-tests applied to 11 kinds of indices derived from counts and measurements of the ICs, support the differences between surgical groups, at least in many instances (P less than 0.05 to less than 0.001). It is concluded that the IC is a characteristic and dynamic feature of the nerve terminals and that it may possibly have a role in such phenomena as recycling of synaptic vesicles or related membrane constituents. It is also concluded that significant time-of-day and neuroendocrine effects are demonstrable in these structures, and that the time-of-day effects shown in these chronic studies have importance in the design of acute experiments designed to further test the functional relations and importance of ICs.

Adrenal Medulla↗
PubMed1979

An experimental study on histamine H2-receptor antagonist on calcium, gastrin and histamine induced gastric acid secretion in rat.

The suppressive effect of histamine H2-receptor antagonist, cimetidine, on gastric secretion was investigated in Ghosh-Schild rat. The study above was done in basal state under infusing normal saline (1 ml/h) and stimulated state by histamine-di-chloride (3.5 mg/kg-h), tetragastrin (50 mcg/kg-h) or calcium chloride (4 mg/kg-h). Dose related increase of cimetidine (1.7, 3.5, 7.0 and 14.0 mg/kg-h) were observed and correlated with the degree of inhibition of acid secretion. Cimetidine had a potent inhibitory activity on either basal and stimulated acid output by the agents above. Basal acid secretion was completely abolished by 3.5 mg/kg-h of cimetidine to the level of anacidity. The degree of inhibition by the same dose of cimetidine was different among the agents used as stimulant on acid secretion and it followed in the order of calcium, gastrin and histamine subsequently. This study indicated that histamine H2-receptor participated the gastric secretion induced by either gastrin or calcium other than histamine itself. This fact indicated the important role of endogenous histamine in gastric secretion induced by calcium and gastrin.

Animals↗
PubMed1979

Daily rhythmic changes in synaptic vesicle contents of nerve endings on adrenomedullary adrenaline cells, and their modification by pinealectomy and sham operations.

Nerve endings on adrenaline cells in male rat adrenal medullas were investigated by quantitative electron microscopy, with sampling at 8 times during a standard light: dark (12:12) photoperiod. Number (N) per unit area of 2 vesicle types (small clear vesicle, SCV; large granular vesicle, LGV) and % of LGV per total vesicle N were determined. Normal (non-operated) animals showed a circadian rhythm (p less than 0.005) in mean SCVN and one with nearly opposite phase relations in % LGVN. Doubly sham-operated animals had a similar rhythm in %LGVN but diminished circadian change in SCVN. Pinealectomized animals had a circadian pattern in SCVN, but this differed from that of normals in having a 2nd peak, a delay or phase shift of the primary peak, and an increased amplitude of the rhythmic changes.

Adrenal Medulla↗
PubMed1979

Demonstration of circadian rhythm in granular vesicle number in pinealocytes of mice and the effect of light: semi-quantitative electron microscopic study.

Adult (70-100 days) male dd-mice were killed at 7 a.m., 1 p.m., 7 p.m. and 1 a.m. under controlled daily photoperiods (L: 7 a.m.-7 p.m.) and temperature, and at 6, 12 and 18 hours after exposure to continuous lighting which started at 7 p.m. Montage photographs were made from electron micrographs at x8200 based on mesh hole areas, using either 150 or 300 meshes. The number of granular vesicles per unit area was counted (GV Score). The GV Score in distal, mid and proximal portions of the body of the pineal gland showed marked 24 hour change, with high values in the late light period and low values in the dark period. The GV Score in the Golgi region showed a 24 hour change with a smaller amplitude. A peak level was attained at mid-light period and low values during a dark period. Additional lighting inhibited the nocturnal decrease in the GV Score in both the whole cytoplasm and the Golgi region, resulting in a slight increase. It is concluded that the number of granular vesicles in mouse pinealocytes (and in their Golgi regions) follows a 24 hour rhythm, and that the nocturnal decrease in the vesicle number depends upon darkness.

Animals↗
PubMed1978

Hypothalamic dopamine-beta-hydroxylase activity: fluctuations with time of day and their modifications by intracranial surgery, adrenalectomy, and pinealectomy.

Hypothalamic and plasma dopamine-beta-hydroxylase [DBH, EC 1.14.2.1] activites were measured by a coupled radioenzymatic method. Animals representing five experimental groups [intact controls, adrenalectomized, pinealectomized, adrenalectomized pinealectomized, doubly sham-operated] were killed and sampled at 8 times through the 24-hr daily cycle, 15 days postoperation, and at 50-52 days of age. Hypothalamic DBH in intact control animals had statistically significant fluctuations in relation to time of day. These changes were lost or dampened in groups that had had intracranial surgery and were characteristically shifted by adrenalectomy, either alone or with pinealectomy. Plasma DBH fluctuations in the same animals resembled those in hypothalamus in some features (e.g., peak near mid-dark; shift in daily maxima and minima after adrenalectomy) and differed in others [e.g., no effect of intracranial surgery or of sham operation; adrenalectomized pinealectomized animals resembled the solely pinealectomized]. Although temporal patterns in hypothalamic DBH activity thus differed in the experimental animal groups, the daily means of hypothalamic DBH activity were similar.

Adrenalectomy↗
PubMed1977

Experimental carcinoma of the biliary tract induced in dogs by N-ethyl-N'-nitro-N-nitrosoguanidine.

The present study was designed to produce the experimental carcinoma of the biliary tract in dogs. Tube cholecystostomy was constructed in 8 mongrel dogs and 5-10 ml of 0.7-1.0 mg/ml solution of N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG) was administered through the tube every day for the maximum period of 180 days. As the results: The experiment had to be cut off in 7 dogs (5 dogs: The tube was inadvertently pulled out. 2 dogs: died of general weakness). Pathological changes were observed in one dog given ENNG for 180 days and sacrificed at 372 days after the beginning of the experiment. Macroscopically, scattered foci of flat elevation of the mucosa were observed in the entire mucosal surface of common bile duct and a tiny polypoid lesion at the terminal protion. A tiny polypoid projection was adenocarcinoma confined to the mucosa, and areas of flat elevation showed marked hyperplasia of mucosa with partial atypical proliferation. No remarkable findings were noted in other organs.

Adenocarcinoma↗