Search PubMed⌕ Search

Biomedical subjects

T Johnsson

Publications and source records attributed to T Johnsson.

51 records · Page 3Linked to original sources
PubMed1976

Immunoelectroosmophoresis for detection of reo-like virus: methodology and comparison with electron microscopy.

In two separate studies, faecal samples were collected from children with acute gastroenteritis; the samples were tested both by electron microscopy and immunoelectroosmophoresis with a view to detect reo-like virus. In 94 per cent and 100 per cent of samples positive by electron microscopy in the first and second material, respectively, a precipitin line was found after electrophoresis, using a guinea pig antiserum against a purified bovine virus. No false positives were detected by this method.

Child↗
PubMed1976

Occurrence of reo-like calf viruses in young children with acute gastroenteritis. Diagnoses established by electron microscopy and complement fixation, using the reo-like virus as antigen.

In the course of a six-month-study of acute gastroenteritis in children of ages up to six years, a reo-like virus was found in 54 per cent of the faecal specimens obtained at an early stage of the disease, using electron microscopy as screening test. By means of a concentrated complement fixation antigen, composed of a related calf diarrhoea virus cultivated in tissue culture, the rise in titre was found to be significant in 96 per cent of the patients whose faeces contained the reo-like virus. Antibodies were present in the remaining 4 per cent without rise in titre. In 10 per cent of the cases with gastroenteritis infection was caused by adenovirus or Salmonella. A probable aetiological agent was found in 71 per cent of the patients. It applies to 33 per cent of all cases caused by the reo-like virus that they were nosocomial infections.

Acute Disease↗
PubMed1975

Demonstration by immunoelectro-osmophoresis of precipitating antibodies to a purified rubella virus antigen.

The nonionic detergent Triton X-100 was used to extract antigens of rubella virus from infected tissue culture cells. Three virus-specific antigens were demonstrated by crossed immunoelectrophoresis by using a pool of human gamma globulin as antiserum. The most dominant of these antigens were purified by ion-exchange chromatography on diethylaminoethyl-cellulose. This antigen was of glucoprotein nature and had slow electrophoretic motility and low binding capacity to diethylaminoethyl-cellulose. Thus, it seems likely that the antigen is identical with the precipitating antigen of rubella virus designated b-antigen or tro-osmophoresis with precipiting antibody in sera obtained from patients recovering from acute postnatal rubella. The precipitin reaction that could be correlated to the hemaglutination-inhibition titers of the same sera appeared 12 days after onset of the disease and remained positive for several years.

Animals↗
PubMed1974

Evaluation of a solid phase radioimmunoassay (Ausria-125) for the detection of hepatitis B antigen (Australia antigen) in two clinical materials.

A solid-phase radioimmunoassay (Ausria-125) was compared to an immunoelectro-osmophoresis method in detecting hepatitis-B antigen (HBAg). The radioimmunoassay detected HBAg in approximately 230 times higher dilutions than the immunoelectroosmophoresis method. In cases of acute hepatitis HBAg could be detected for about 70% longer after the onset of jaundice using radioimmunoassay compared with immunoelectroosmophoresis. Of 138 patients with acute hepatitis, who were HBAg-negative by immunoelectroosmophoresis, 23 (17%) were positive by radioimmunoassay. The specificity of the positive results with this test was investigated by neutralization tests with human antiserum to HBAg.

Animals↗