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Biomedical subjects

T J Muckle

Publications and source records attributed to T J Muckle.

At least 37 records · Page 2Linked to original sources
PubMed1978

Electrophoretic bimorphism of serum albumin in the presence of indocyanine green.

The presence of indocyanine green during extended traditional electrophoresis and immunoelectrophoresis of serum is associated with bimorphism of albumin. This occurs over a range of dye--albumin molar ratios an order or more greater than was obtained in similar phenomena described previously. The bimorphism seems not to be dose dependent beyond a certain point, and the two albumins so separated show tinctorial differences. The phenomenon has been observed to apparently the same degree in all normal sera tested, and may represent a means of distinguishing ligand-loaded and ligand-light serum albumin.

Blood Protein Electrophoresis↗
PubMed1978

Suntan localization of varicella exanthem.

The case history of a Caucasian child in whom the exanthem of varicella appeared to be related only, and directly, to the darkness of skin suntan is presented and discussed.

Chickenpox↗
PubMed1977

The "post-heparin pattern" in routine serum protein immunoelectrophoresis.

For some time following intravenous administration of heparin to non-fasting normal subjects, routine immunoelectrophoretic preparations show characteristic changes in serum lipoproteins. These alterations are of two types: (1) marked anodal mobility increase, the alpha-2 (beta) component locating in the alpha-1 region while the alpha-1 appears as a pre-albumin; (2) conformational alterations of the immunoprecipitin arcs comprising elongation of the alpha-2 (beta) arc, and splitting of the alpha-1 arc into two distinct entities. Familiarity with these patterns should minimize the possibility of interpretative errors with specimens from patients receiving heparin.

Blood Protein Electrophoresis↗
PubMed1977

Characteristics of the immune response in a patient with Whipple's disease.

A patient with Whipple's disease has been studied to examine the effect of antibiotic therapy on the immune status of the patient, and the specific immune response to a cell wall deficient form of an alpha-haemolytic streptococcus (alpha HS) isolated from this patient. T lymphocyte numbers were reduced, and cutaneous anergy was present. Autoantibodies directed against smooth muscle and mitochondria were detected. These abnormal parameters became normal following antibiotic therapy. The specific immune response to the alphaHS was characterised by IgA antibody and lymphocyte sensitisation. The latter was detected as antigen-inducedd lymphocyte stimulation and antigen-induced leucocyte inhibition factor (LIF) production. Antibiotic therapy was associated with a fall in antibody titre and reduced LIF production. No defect in neutrophil function was found. These results are most consistent with the postulates that (i) immunological abnormalities in Whipple's disease are secondary to infection and (ii) the primary abnormality is an unusual pathogenic bacterium.

Adult↗
PubMed1977

Influence of oleic acid on serum lipoprotein-X in vitro.

Lipoprotein-X is no longer detectable in serum by either the agar gel-electrophoresis/polyanion precipitation technique or immunoelectrophoresis with specific antiserum after in vitro addition of oleic acid. Evidence is presented which indicates that this ostensible loss is due not to its destruction, but rather to altered electrophoretic mobility. The findings suggest an explanation for the well known post-heparin "disappearance" of lipoprotein-X, and indicate that caution may be needed in the interpretation of such lipoprotein-X testing of sera from subjects with increased concentrations of lysolipids in their blood.

Adult↗
PubMed1976

Changes in the immunoelectrophoretic characteristics of serum alpha-I-lipoprotein induced by various dyes.

The effect of 8 dyes on the electrophoretic mobility of normal human serum alpha-1 lipoprotein has been examined. The two characteristics of increase in anodal mobility, and conformational changes of the immunoelectrophoretic precipitin arc suggesting polymorphism, were found to vary independently among the dyes tested. The results suggest that dye induced electrophoretic mobility alteration may represent a useful tool for examination of heterogeneity of alpha-1 lipoprotein.

Coloring Agents↗
PubMed1975

Isolation and characterization of an aetiological agent in Whipple's disease.

A cell wall deficient form of an alpha-haemolytic streptococcus was grown from a prolonged monolayer cell culture of a lymph node taken from a patient with Whipple's disease. Serological cross reactivity was shown between the organism and the material within Whipple's disease macrophages positive for diastase-resistant periodic acid-Schiff (D./P.A.S.). In vitro studies characterized the organism as a facultative intracellular parasite which caused the accumulation within cells of D./P.A.S.-positive material. These results suggest that a pathogenic bacterium is the essential aetiological agent and that the culture of Whipple's disease tissues in hypertonic media may have practical value.

Adult↗
PubMed1975

Progressive combined amyloid-elastic stain.

A progressive staining technique is described that will permit demonstration of elastic structures and amyloid deposits in the same section. It is satisfactory for animal as well as human tissues, and for both pericollagen and perirecticulin types of amyloidosis.

Amyloid↗